Life Sciences Commons^™

The Effects Of Estradiol And Thyroxine On The Plasma And Liver Proteins Of The Thyroidectomized Water Snake, Albert D. Morrongiello

Master's Theses

The effects of thyroidectomy, estradiol treatment, and thyroxine treatment on plasma and liver protein concentrations in the water snake, Natrix fasciata fasciata (Linnaeus, 1766), were investigated. There was a significant lowering of liver protein concentrations following thyroidectomy. This deficit could be replaced with thyroxine alone, but could not be replaced by treatment of thyroidectomized snakes with estradiol alone or with a combination of estradiol and thyroxine.

The plasma resolved into nine plasma proteins when electrophoresed in polyacrylamide gels. Although thyroidectomized snakes treated with estradiol only or a combination of estradiol and thyroxine showed enhancement in two protein fractions, the significance …

Ddt Inhibition Of (Na+, K+)-Activated Atpase In Gill Tissue Of Fundulus Heteroclitus, Nancy Mccutcheon Slate

Master's Theses

The DDT inhibition of (Na+, K+) - activated ATPase in gill preparations of the euryhaline teleost, Fundulus heteroclitus, was measured. The (Na+, K+) -activated ATPase was found to be sensitive to DDT, with 74.6% of the enzyme's specific activity inhibited by 20 ppm DDT. The (Mg++)-activated ATPase was also sensitive to inhibition bv DDT but not to the extent of the (Na+, K+) - activat ed ATPase . Due to previous work which showed that DDT antagonized valinomycin-induced K+ conductance in a synthetic membrane, the effect of K+ on DDT inhibition was examined kinetically. The Km for K+ in gill …

Isolation And Identification Of The Toxic Principle In The Tissues Of The Ectoproct Lophopodella Carteri (Hyatt), Donald Coleman Smith

Master's Theses

The tissue of Lophopodella carteri (Hyatt) was isolated, and the agent toxic to fish present in the tissues was extracted. A chloroform-soluble component and a water-soluble component were separated from the filtrate of the homogenized tissues. Each component was tested for toxicity. The chloroform-soluble component was found to be extremely toxic. The water-soluble component was originally non-toxic, but after several months of freezing and defrosting the tissue, this component became toxic.

The chloroform-soluble component was purified by thin-layer chromatography. Several lipid classes were obtained, of which one was toxic. This toxic class was further purified by thin-layer chromatography resulting in …

Spacing And Orientation Of The Siphons Of The Tunicate, Styela Plicata, Alfred Page Chestnut

Master's Theses

The spacing and orientation of sipons of Styela plicata (Lesueur)in swift and slow currents were examined. Within aggre­ crates, the siphons were spaced farther apart in slow currents than in swift currents. The siphons were not horizontally oriented but were vertically oriented in slow currents. The adaptive values of spacing and orientation under different currents are discussed.

An Electrophoretic Comparison Of The Gill Plasma Membrane Proteins Of Freshwater And Saltwater-Adapted Killifish, Fundulus Heteroclitus, Earl Dean Hammit

Master's Theses

A study was undertaken to compare proteins present in the gill membranes of freshwater and salt­ water adapted killifish, Fundulus heteroclitus. For this purpose, a method for the preparation or gill plasma membranes was developed. Polyacrylarnide gel electrophoresis indicated that the two types of gills possessed many membrane proteins in common. However, the freshwater adapted group possessed a single protein not found n the saltwater adapted group and the latter contained a protein not found in the freshwater adapted group.

Quantitative Growth Relationships Of Microcoleus Vaginatus (Gom.) Ex Drouet And Alcaligenes Sp. In A Sucrose-Salts Medium, James Rexford Brownell

Master's Theses

The blue-green alga, Microcoleus vaginatus (Gom.) ex Drouet 1968, and a bacterium, Alcaligenes sp., collected from the same habitat, were cultured together and separately in mineral salts media with and without sucrose. Growth of axenic M. vaginatus was stimulated by sucrose. In association with the bacterium in sucrose-containing media algal growth was suppressed, while in inorganic media it was stimulated. Factors involved in this algal-bacterial system probably included competition for the carbon source, changes in pH, and accumulation of cellular waste and lytic products.

The Role Of The Adrenal Gland In The Survival Of White Rats Subjected To The Parotid Toxin Of The Toad, Bufo Marinus, Mark A. Green

Master's Theses

Six groups of white labor atory rats were utilized in this experiment. A sham-operated group and an adrenalectomized group were used as controls. Four other groups were adrenalectomized and given replacement therapy of cortisone, deoxycorticosterone acetate, epinephrine, and norepinephrine. All groups were then given injections of toad toxin from the parotid glands of Bufo marinus. Their heart and breathing rates were recorded for 60 minutes.

All of the untreated adrenalectomized rats showed altered heart and breathing rates and were killed by the toxin. Cortisone and nor ­ epinephrine aided the adrenalectomiz ed rats to survive. but cortisone was the …

A Survey Of Fresh-Water Oligochaeta And Their Commensal Ciliates From The Richmond, Virginia Area, Elsa Queen Falls

Master's Theses

The distribution of North American aquatic oligochaetes and the ciliates inhabiting their guts have received very limited attention. For this reason, the present study was initiated to survey fresh ­water oligochaetes of the families Aeolosomatidae, Naididae, Opistocystidae, and Lumbriculidae in four bodies of water of the Richmond, Virginia area and to determine the incidence of commensal ciliates within the gut .

Copulation In Two Species Of Tetraopes: Behavior, Mechanics And Morphology (Coleoptera, Cerambycidae), Thomas John Pilat

Master's Theses

No abstract provided.

The Formation And Constriction Of The Trapping Rings In The Nematophagous Fungus Dactylaria Brochopaga Dreschler, Walter Thomas Rudek

Master's Theses

No abstract provided.

Effect Of Nutrients On The Growth Of Cyanophyta Species And Their Ability To Develop Virus Infection Under Different Physiological Conditions, Vithalbhai Lavjibhai Sheladia

Master's Theses

No abstract provided.

Electron Microscope Study Of The Infection Process Of Blue-Green Algae Virus, Thomas A. Rudd

Master's Theses

No abstract provided.