Life Sciences Commons^™

Role Of Msaa Gene In Regulation Of The Msaabcr Operon And Biofilm Development In Staphylococcus Aureus, Ahmed Alzuway

Master's Theses

Staphylococcus aureus is an important human pathogen that causes wide variety of diseases ranging from chronic biofilm associated infection to acute life threatening infection such as bacteremia, pneumonia, osteomyelitis, or endocarditis, despite the progress with antibiotics used in the treatment of bacterial infections. Furthermore, increased use of prosthetic and indwelling devices in modern medical practices has led to increased infections due to S. aureus. Treating S. aureus infections have become difficult owing to its ability to resist most of the antibiotics; this problem is further exacerbated by ability of MRSA strains to form biofilms. Emergence of community-acquired methicillin resistance …

Effects Of Water Parameters On Container Mosquito (Diptera: Culicidae) Oviposition And Performance, Stephanie Sue Schelble

Master's Theses

Water body parameters have a considerable effect on the communities that develop within them. In small container habitats like tires, the depth, surface area, and volume effect the development and success of the mosquito communities. This study investigated the choices of the adult female mosquitoes, Aedes albopictus and Culex quinquefasciatus, between different depths and surface areas. In addition, larval performance was determined under differing depth and larvae densities. Oviposition studies showed that Ae. albopictus had a preference for deeper habitats (χ²= 14.2902, p= 0.0139) but did not prefer any surface areas (χ²= 7.2321, p= 0.0649) though …

Self-Assembled Gold Nanoplexes For Cancer-Targeted Sirna Delivery, Yongliang Shi

Master's Theses

Through layer-by-layer method, the authors have constructed three Au nanoplexes: AuPEI/RNA/PEI, AuPEI/RNA/PEI-mPEG, and AuPEI/NA/PEI-PEG-FA. All the nanoplexes are characterized by UV-vis spectrometry, DLS, and zeta potential. The surface density of the first layer PEI and second layer RNA were also determined. Moreover, the Au nanoplexes can protect siRNA from RNase degradation and are stable in cell culture medium.

siGLuc-ppp, ssRNA₈₀, and siF17 were delivered by the assembled Au nanoplexes, and the results were analyzed by GLuc assay, TB assay, and Luciferase assay. In terms of RNA delivery, the Au nanoplexes AuPEI/RNA/PEI exhibited excellent gene silencing efficiency (or causing …

The Role Of Carotenoid Cleavage Dioxygenase 4 In Flower Color Of The Allopolyploid Brassica Napus, Leanne Denice Fogg

Master's Theses

Allopolyploids are formed by interspecific hybridization and whole genome duplication, with the resulting organism contains multiple distinct subgenomes in one nucleus. Subgenomic interactions result in massive genetic and epigenetic reconstruction, contributing to variable phenotypic traits noted in newly formed allopolyploids. To better understand these mechanisms in the context of molecular biology, evolution, and plant breeding, plant biologists study the model organism Brassica napus (farmed as canola or oilseed rape). With white-flowering and yellow-flowering progenitors, flower color phenotype of B. napus presents an opportunity to examine subgenomic interactions. CAROTENOID CLEAVAGE DIOXYGENASE 4 (CCD4) is known to play a major …

Proliferation, Migration, And Survival Of Cells In The Telencephalon Of The Ball Python, Python Regius, Thomas B. Bales

Master's Theses

Reptiles exhibit neurogenesis throughout the brain during adulthood. However, very few studies have quantified telencephalon-wide neurogenesis in adulthood, and no studies have performed these investigations in snakes. Quantifying neurogenesis in the adult snake is essential to understanding class-wide adult neurogenesis and providing insight into the evolution of this trait. The thymidine analog 5-bromo-2’-deoxyuridine (BrdU) was used to quantify cell proliferation, migration, and survival in the ball python (Python regius). First, to determine the proper dose of BrdU for injection we subcutaneously injected 50mg/kg, 100mg/kg, and 250mg/kg into 15 adult male P. regius. We found the 250mg/kg dose …

Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes

Master's Theses

The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual examination of suspicious regions …

Effectiveness Of A Football Over Helmet Padding System In Reducing Peak Acceleration Of The Head And Severity Index 2014, Nicole Cuccurullo

Master's Theses

The purpose of this study was to determine if: (a) a football helmet equipped with the Guardian Cap meets the National Operating Committee on Standards for Athletic Equipment (NOCSAE) football helmet standards and (b) if the Severity Indexes and peak accelerations produced during the NOCSAE impact tests were smaller for a football helmet equipped with the Guardian Cap over-helmet padding system than for the same helmet without the Guardian Cap. A total of 54 drop impact tests were completed, 27 on the football helmet alone and 27 on the football helmet equipped with the Guardian Cap. Tests were completed on …

Assessing Rodent Species Counts And Diversity In The Not-Grazed Montana De Oro State Park And The Rotationally Grazed Pecho Ranch, Nancy Marie Lemos

Master's Theses

The available data examining the influence of rotational grazing on rodent responses are limited. This study investigated how rotational livestock grazing practices influence small mammal rodent abundances and species diversity. We looked for evidence of variation in the occurrence and/or numbers of certain rodent species among three plant community types (grassland, shrubland, grass-shrub mix), managed with or without grazing. We used Sherman live traps over a total of eight trapping sessions. We totaled 486 trapnights for each of the six plant community and grazing management combinations. The three plant community types were identified by visual cover.

Out of the eight …

Monarch Butterfly (Danaus Plexippus) Tree Preference And Intersite Movement At California Overwintering Sites, Jessica Lynn Griffiths

Master's Theses

Managing Monarch butterfly overwintering groves: making room among the eucalyptus

Proper management and conservation of the coastal California overwintering sites used by western Monarch butterflies (Danaus plexippus L.) is critical for continued use of these sites by monarchs. Many management efforts are currently concentrating on eucalyptus-only sites because of the prevailing notion that monarchs prefer eucalyptus over native tree species. Yet, whether a preference exists or not has never been tested. Herein, we test the “eucalyptus preference” hypothesis with data from five overwintering sites comprised of blue gum eucalyptus (Eucalyptus globulus) and at least one other native tree …

The Role Of Sirtuin Inhibitors On The Proteomic Responses Of The Mussels Mytilus Galloprovincialis And Mytilus Trossulus To Menadione Induced Oxidative Stress, Hayley C. Chilton

Master's Theses

Global climate change imposes physiological constraints on marine ecosystems that can alter the distribution of intertidal organisms. In one such instance, the native cold-adapted mussel Mytilus trossulus is being replaced along its southern range by the invasive warm-adapted Mytilus galloprovincialis. These blue mussels occur throughout rocky intertidal zones where they are subjected to greatly varying environmental conditions known to induce oxidative stress. We hypothesize that while under acute stress, related Mytilus congeners undergo a shift in redox potential from NADH-fueled respiratory pathways to pathways producing NADPH as a way to decrease the production of reactive oxygen species (ROS) and …

Optimization Of Growth Parameters For Algal Regrowth Potential Experiments, Chadwick D. Boggess

Master's Theses

Water cost is one of the key deterrents in making algal biofuels a feasible alternative to traditional petroleum fuels. Research into recycling of algal media was conducted in a laboratory setting at the California Polytechnic State University in San Luis Obispo. A growth assay was created to allow for algal cultures, specifically Ward’s Chlorella sp., to proliferate in sealed culture vessels. Bold’s Basal Medium (BBM) was selected as the media due to its extensive use with freshwater algal strains. However, this medium can limit algal growth through nutrient limitation and it became necessary to augment the original recipe. 50mM …

Investigation Of Chromosome Size Effect On The Rate Of Crossovers In The Meiotic Yeast Saccharomyces Cerevisiae, Lanie Maria Galland

Master's Theses

Meiosis is a specialized type of cell division characterized by a single round of DNA replication and two rounds of chromosome segregation, ultimately resulting in four haploid cells. During meiosis I, chromosomes align and reciprocal recombination results in the formation of a crossover, creating the tension required to properly segregate homologs during the first round of meiosis.

Two mechanisms involved in regulating the occurrence of crossing over are assurance and interference. Crossover assurance describes the phenomenon that at least one crossover will form between each pair of homologous chromosomes during prophase I. Crossover interference, on the other hand, describes the …

An Examination Of An Environmental Change In Beverages Available To High School Students In Santa Maria, California On Their Consumption Behavior, Susan Eileen Klucker

Master's Theses

Hypothesis: "By an environmental change in high school vending machines, making water available, students will choose water over the sugar sweetened sodas."

This document highlights a case study analysis of vendor-provided refill data for forty-five beverage vending machines at two campus sites in one high school district in 2003 and 2004. The innovative study and publicized negotiated soda contract of a 50:50 (healthy to unhealthy) beverage ratio stipulation became the “Win-Win-WEAN” compromise, in which exposure to healthier beverage options for students might prove to provide the same income opportunity for the school district.

An overview of the political …

Photosynthetic Thermal Tolerance And Recovery To Short Duration Temperature Stress In Desert And Montane Plants: A Comparative Study, David William Gallagher

Master's Theses

• Climate change models predict an increase in frequency and amplitude of extreme weather events, including heat waves. To better predict how the composition and distribution of plant assemblages might respond to these changes in temperature, it is important to understand how species currently respond to these extremes. Photosynthetic thermal tolerance (T₂₅)and photosynthetic recovery (RT₂₅) were quantified in 27 species. We also studied the relationships between T₂₅, RT₂₅ and leaf mass per area (LMA). Leaf temperature was also monitored in the field.
• Leaves used in this study were collected from two distinct …

The Quadmill: Effects Of Eccentric Training Vs. Concentric Training On Lower Extremity Power And Anaerobic Capacity 2014., John Crosby

Master's Theses

Training focuses on the concentric action of muscle during exercise for most exercise programs, but eccentric training may yield greater results. The Quadmill^TM is a unique piece of training equipment that focuses on training the quadriceps muscle eccentrically. Purpose: to determine if using the Quadmill^TM could increase power and anaerobic capacity to the same level, if not greater, than a standard concentric focused lower body training protocol. Participants were 44 undergraduate college students (24 Male 20 Female) Design: participants placed into three equal groups (Quadmill^TM, Lifting, Control). The two experimental groups (Quadmill^TM and Lifting) underwent …

Physical Activity And Nutritional Behavior Of Overweight College Students Trying To Lose Weight 2014, Santino Vallejos M.S.

Master's Theses

Although there are numerous benefits to increased physical activity and proper dietary behaviors, the decreased participation in physical activity and poor dietary behaviors has emerged as a significant problem within the college student population. The focus of this research study was to identify the relationship between physical activity and dietary behavior to weight loss behaviors within an undergraduate student sample of a small, comprehensive northeastern university. College-aged students (age range: 17 to 27 years old) completed the American College Health Association’s (ACHA) National College Health Assessment II (NCHA) during the years 2009 and 2012. According to the results, there were …

"Can’T Touch This": Empowering Children With Severe Food Allergy In The Residential Camp Setting 2014, Alexsandra Dubin

Master's Theses

The purpose of this project was to create an empowerment program for children with severe food allergy (SFA) in a residential camp setting. Despite recent advancements in research, there is no cure for SFA, thus there is a need for programs to help children manage their allergies and cope with the ramifications of their condition. Quality of life is a major concern for children with SFA, as the constant fear of exposure to an allergen can lead to high levels of anxiety. This is particularly noteworthy because, barring exposure to an allergen, these children are otherwise healthy and symptom free. …

Factors Affecting The Coefficient Of Friction Of Soccer Goalie Gloves 2014, Candice Hofmann

Master's Theses

The purpose of this study was to compare the coefficients of static friction of five different pairs of soccer goalie gloves with one soccer ball and to compare the effects of four different glove conditions on the coefficients of static friction. Specifically, the coefficients of static friction were determined and compared for one size 5 soccer ball (Select) with five different pairs of goalie gloves (Nike Vapor Grip 3, Select 88, Uhlsport Aqua, Uhlsport Soft and Uhlsport Hard) and four different glove conditions (new, wet, saliva and dirty). Surrogate wooden fingers were inserted into each glove and the glove was …

Resident Benthic Fishes Of Artificial Reefs In The Mississippi Sound: Effects Of Habitat Relief And Subregion, Claire Louise Matten

Master's Theses

One of the fundamental questions of artificial reef research concerns the capability of these manmade structures to promote secondary production. Many researchers have questioned whether artificial reefs increase the production of fish biomass, or simply aggregate existing fish biomass. Most previous research has focused on production of transient fish biomass, because of the high recreational and commercial value of these species. Establishing a link between transient fish production and artificial reef primary and secondary production has proved difficult. Therefore, the goal of this study was to examine the productivity of benthic fishes resident to artificial reefs in the Mississippi Sound, …

Taxonomy, Diversity, And Distribution Patterns Of Portunid Crab Megalopae In The Northern Gulf Of Mexico During Fall Of 2003, Carley Rain Knight

Master's Theses

The field of zooplankton biology contributes to more accurate stock assessments as well as to a greater understanding of the marine food web. However, adequate information for the invertebrate component of zooplankton is lacking compared to the ichthyoplankton component. In this thesis, identification of Portunidae (Crustacea: Decapoda) megalopae collected during the fall of 2003 from a NOAA SEAMAP cruise revealed 7 species and 11 morphs with 90% of the total density comprised of Callinectes sapidus, Achelous gibbesii, Callinectes similis, Achelous spinicarpus, and Achelous sp.I. Keys and detailed descriptions are provided along with photographs and morphological drawings for each morph to …

Three-Dimensional Reconstruction Of Marine Clay Nano- And Microstructure By Transmission Electron Microscopy: Analysis Of Fabric And Pore Network, Jessica Rae Gardner Douglas

Master's Theses

Three-dimensional reconstructions of marine fine-grained sediment open a new dimension for studying nano- and microscale organo-clay fabric important to improving and advancing organic matter (OM) sequestration and fluid flow dynamics modeling. I created 3-D reconstructions of clay fabric and pore pathways from serial sections and photographic mosaics obtained using transmission electron microscopy. These reconstructions show aggregations of clay domains, surrounding OM, and pore networks. I examined a Model sample (laboratory consolidated) with 1% OM and a Natural sample (polychaete fecal pellet) with high levels of OM. Three-dimensional reconstructions were segmented into 300 nm cube subsamples to make highly localized qualitative …

Propagation Of Oligomeric Α-Synuclein And Amyloid-Β: Implications For Parkinson's And Alzheimer's Diseases, Matthew Stephen Planchard

Master's Theses

The aggregation of amyloidogenic proteins is a critical event in the pathology of a variety of neurodegenerative diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). The proteins α-synuclein (αS) and amyloid-β (Aβ) are involved in the formation of amyloid lesions observed in PD and AD, respectively. Both PD and AD exhibit a significant amount of co-pathology in clinical settings, and the αS and Aβ proteins have been shown to interact in vitro. Recent experimental consensus has shown oligomeric species to be significant, if not primary, sources of toxicity in these diseases. …

Evaluation Of Red Alder Mortality In The Little Creek Watershed Following The 2009 Lockheed Fire, Dylan Robert Theobald

Master's Theses

Five hundred eighty red alder along a 2.16 km portion of the Little Creek riparian zone were assessed for mortality following the 2009 Lockheed Fire near Davenport, California. The study area was divided into burn severity zones and every red alder within the riparian zone was observed and assessed for mortality. Height, diameter at breast height (DBH), and age were collected on selected trees. An estimation of red alder large woody debris (LWD) input to Little Creek since a 2010 LWD survey (Smith, 2010) was calculated using average red alder tree dimensions applied to Smalian’s formula (MLNRO, 2011). The mean …

Territorial Behavior And Cortical Brain Plasticity In Adult Male Sceloporus Occidentalis, Daniel R. Pfau

Master's Theses

The hippocampus is a brain region that can undergo tremendous plasticity in adulthood. The hippocampus is related to the formation of spatial memories in birds and mammals. In birds, plasticity in the hippocampus occurs when formation of such memories is directly relevant to survival or reproduction, such as for breeding or food caching. In reptiles, the homologues to the hippocampus are the dorsal and medial cortices (DC and MC). In several lizard, snake and turtle species, these structures have been related to spatial memory. Experimental investigations indicate that differences in DC volume are related to space use associated with differing …

The Ecological Effects Of Cattle Grazing On Reptiles And Small Mammals In A San Joaquin Valley Grassland, Michael William Tom

Master's Theses

Livestock grazing is a common and extensive land use practice in the United States occurring in a wide range of habitat types. As such, livestock grazing has the potential to alter ecosystem structure, function and community composition. The primary component (Chapter 1) of this thesis examined the effects of cattle grazing in a San Joaquin Valley grassland on two target taxa: reptiles and small mammals. The study took place on the Chimineas Unit of the Carrizo Ecological Reserve, San Luis Obispo County, California during Fall 2009 and Spring 2010. These taxa were sampled on matched pairs of two grazed and …

Effects Of Urbanization On Sediment Microbial Communities In Lotic Ecosystems, Bradley Drury

Master's Theses

The world is becoming increasingly urbanized, with the majority of the world's population now living in urban areas. Urbanization has the potential to significantly alter lotic ecosystems and the services they provide. Benthic microbial communities are key components of lotic ecosystems due to their contributions to primary production and nutrient cycling. Two types of human inputs associated with urbanization that may impact benthic microbial communities in lotic ecosystems are the input of wastewater treatment effluent and the input of emerging contaminants, including pharmaceuticals and personal care products. This work examines the effects of treated WWTP effluent on benthic microbial communities …

Functional Characterization Of A Novel Thioredoxin Domain-Containing Protein Of The Malaria Parasite Plasmodium, Rachel Kooistra

Master's Theses

A novel thioredoxin domain-containing protein of the malaria parasite Plasmodium was identified and found to be conserved among eukaryotes. This protein belongs to the phosducin-like family of proteins (PhLPs), and was therefore assigned the name PhLP1, since it is the first phosducin-like protein to be identified in Plasmodium. PhLPs have been found to have various roles in G-protein signaling, cell cycle progression, and protein folding. However, the biochemical mechanism by which PhLPs perform their function is unknown. Here is described the cloning and biochemical characterization of both PhLP1 and its human homolog TXNDC9. Both purified PhLP1 and TXNDC9 showed enzymatic …

Role Of The Intestinal Microbiota In Gut Barrier Dysfunction Following Burn Injury, Zachary Earley

Master's Theses

Burn injury represents a major medical problem with half a million cases requiring medical attention and 4,000 deaths reported annually. Sepsis and multiple organ failure remain the leading causes of death following injury, and may be brought on by bacterial infections or toxins. The gastrointestinal tract contains approx. 100 trillion microbes; therefore, the indigenous commensal microbiota may play a role in leading to these complications or infections in burn patients. The overall objective of this project is to identify a potential mechanism whereby changes in gut bacteria may lead to intestinal inflammation or bacterial translocation--key factors which may lead to …

Environmental Drivers Of Leaf Breakdown Rate In An Urban Watershed, Ashley Rachelle Cook

Master's Theses

Leaf litter breakdown is a critical ecosystem process in urban streams, but environmental conditions in urban streams may generate confounding effects on breakdown rates. Reduced abundance of macroinvertebrate shredders may slow breakdown, but rates may increase if high nutrient concentrations stimulate microbial decomposers and if flooding enhances leaf fragmentation. We used the litter bag technique to measure the relative importance of multiple environmental drivers on breakdown of eastern cottonwood (Populus deltoides) leaves at 5 sites throughout the North Branch of the Chicago River watershed. Sites spanned a gradient of urbanization, but no specialized macroinvertebrate shredders were present at any sites. …

Proteomic Analysis Of Mammary Epithelial Cell Development, Alyssa Kay Conly

Master's Theses

In this set of studies, a proteomic approach was used to investigate the protein profile of the mammary epithelial cell (MEC) through different stages of mammary development. The HC11 cell line was used to investigate protein changes between undifferentiated and differentiated MEC, which represent the pregnant and lactating states of the cells. This comparison revealed an interesting differential expression profile underscoring many recognized processes that occur in differentiated MECs, while others unveiled differences between MEC differentiation in vitro and in vivo. Primary MEC were also isolated from virgin, pregnant, and primiparous quiescent mice to compare the virgin state of the …