Life Sciences Commons^™

Transcriptomic Analysis Of Honey Bees Exposed To Organosilicone, Matthew Brent Thompson

Undergraduate Honors Capstone Projects

Honey bees play an important role in agriculture and the decline of honey bee populations worldwide has generated concern. While the application of pesticides in agricultural settings is often implicated in the deterioration of honey bee population health, pesticide applications contain more than just pesticides; they also contain adjuvants that may have detrimental effects to bee health. One known effect of one type of adjuvant is the suppression of immunity-related genes and consequent increase of viral load in larvae. We investigate the effects of one class of adjuvant, organosilicone surfactants (OSS), on adult honey bee health. In a laboratory based …

A Review Of Osteoarthritis, Madelin Brooke Session

Undergraduate Honors Capstone Projects

Osteoarthritis is a degenerative joint disease which plagues many older animals and humans. It is a disease that is characterized by the degeneration of joint cartilage, inflammation, as well as chronic pain and stiffness that results from this disorder. Unfortunately, most of the treatments for this painful and chronic disease mostly involve pain management and temporary relief strategies. These strategies usually include pain medication, non-steroidal anti-inflammatory drugs, injectable lubricants, as well as surgical techniques. However, because these treatments are just temporary fixes meant for pain management and to improve quality of life they must continue throughout the remainder of the …

A Qualitative Research Approach To Understanding Challenges That May Inhibit Optimal Usage Of Automatic Milking Systems In Northern Utah, Jessica Christensen

Undergraduate Honors Capstone Projects

Dairy robotics, i.e., Automatic Milking Systems (AMS), is a relatively new field, one that has great promise to optimize efficiency, production, and animal welfare of dairy cattle. However, despite quantitative research findings that indicate AMS success, dairy farmers still face challenges integrating AMS into their production systems. During the fall of 2018, interviews were conducted with northern Utah dairy farmers regarding their robotic systems. The respondent data was analyzed to reveal repeated problems with the robots. This analysis was then used to direct research in order to propose solutions to the farmers' AMS challenges. Conclusions were then summarized in an …

The Influence Of Time Spent In Beginning And End-State Postures On Grasp Choice, Rachel Modersitzki

Undergraduate Honors Capstone Projects

The choice to end comfortably often requires adoption of uncomfortable beginning states, demonstrating that a sequence of movement is planned in advance of movement onset. Many factors may influence the choice of comfortable end-state postures including the greater precision and speed afforded by postures at joint angle mid-ranges (Short & Cauraugh, 1999). There has been no evaluation of the hypothesis that postures are chosen based on minimizing time spent in postures. Typically, the time spent in beginning and end-states are not explicitly constrained, but the end-state posture is likely held for the longer amount of time due to greater precision …

Evaluation Of Antiviral Agents In Two Mouse Models Of Rna Virus Infections, John Hyrum Mcclatchy

Undergraduate Honors Capstone Projects

The first model we are evaluating is human influenza virus in mice. Influenza virus is a negative sense RNA virus, which the CDC estimates causes between 9.2 million and 35.6 million illnesses each year in the United States.^[1] In the current influenza season, H3N2 viruses have been the predominant subclade of influenza A virus in circulation.^[2] Development of a mouse model for influenza H3N2 virus infection has been difficult. In these studies, we endeavored to mouse-adapt influenza A/Hong Kong/4801/2014 H3N2 virus by serial passaging in AG129 mice. This virus strain was chosen as it overlaps in serotype with …

The Vestibular Contribution To Balance Control In Older Adults During Locomotion And Stair Negotiation, Megan Elwood

Undergraduate Honors Capstone Projects

Background: Stability is known to decrease as we age, but currently we know very little about how the body's balance system, the vestibular system, contributes to balance control in older adults, particularly while walking and climbing stairs. The purpose of this study was to take the first step in understanding vestibular contribution to balance control during locomotion and stair negotiation, and how this changes with age.

Methods: Ten young adults and six older adults ascended and descended a nine-step staircase 78 times and walked on a treadmill for 10 minutes to complete a total of 300 complete steps in …

The Effect Of Gender And Socioeconomic Status On Concussion Reporting Behavior Among Ncaa Student-Athletes, Joshua Anthony Hansen

Undergraduate Honors Capstone Projects

Concussions are traumatic brain injuries that result from "brain shaking" that can occur during any situation that transmits force to the head. Concussions are defined as a clinical syndrome characterized by immediate and transient post-traumatic impairment of neural functions which lead to a complex grouping of both psychological and physiological symptoms (McCrory, et al., 2013). As knowledge of the long-term implications of these injuries grows, concussions are becoming more of a major health concern worldwide. One subset of concussion classifications, sports-related concussions, is receiving an increasing amount of attention from both scientists and health-care practitioners. It is estimated that more …

The Role Of Trailing Behavior In Conspecific Mating In Thamnophis Elegans And Thamnophis Sirtalis, Eleanor Watson

Undergraduate Honors Capstone Projects

In many gartersnake species, successful mating depends on the ability of males to follow pheromone trails left by females. The populations we investigated (Thamnophis sirtalis and Thamnophis elegans, closely related sympatric species) overwinter together and simultaneously emerge. Although, mating occurs concurrently, there is no evidence of hybridization. Therefore, we sought to investigate the mechanisms that allow male snakes to differentiate between heterospecific and conspecific females to ensure mating success. Behavior studies were conducted by presenting male snakes with extracted scents of conspecific females, heterospecific females, and conspecific males. We measured male preference by number of investigatory tongue flicks …

Effect Of Wood Chips As A Component Of Soilless Media On Growth And Nutrition Of Food And Ornamental Crops, Kristen Bullough

Undergraduate Honors Capstone Projects

Peat is the central component of the soil-less media mix in all greenhouse crop production but it is expensive because it is harvested in Canada and shipped to greenhouses across North America. Wood chips provide a local, low-cost alternative to peat, but observations by growers indicate potential growth reductions from the addition of wood to peat-based media. Here I report the effects of the addition of wood chips to peat-based media. The study included four treatments: two controls (peat/vermiculite: 50/50 and 75/25) and two treatments with wood chips ' (peat/wood chips: 50/50 and 75/25) with three species (sunflowers, soybeans, and …

Managing Dietary Protein In Cattle As A Means Of Reducing Ammonia Emissions To Improve Air Quality, Ashley Houston

Undergraduate Honors Capstone Projects

Particulate matter pollution has become a subject of great concern across the globe. Emissions data has revealed that the agricultural sector is making large contributions to particulate matter through ammonia emissions. Beef and dairy cattle are responsible for producing nearly 50% of annual ammonia emissions in the United States. These animals are often fed amounts of dietary protein that exceed recommendations, resulting in increased excretion of urea and ammonia. These compounds combine with nitrogen oxides in the atmosphere to form PM_2.5: particulate matter measuring less than 2.5 microns in diameter. Research has shown that through proper dietary management …

Maternal Vitamin D Deficiency And Early Childhood Health Outcomes Including Autism Development, Kelsey L. Girardelli

Undergraduate Honors Capstone Projects

Many studies have shown that vitamin D deficiency during pregnancy is associated with a variety of adverse maternal and pediatric outcomes. Disease outcomes that have been observed in pregnant women who are vitamin D deficient include increased risk of C-section, preeclampsia, bacterial vaginosis, and gestational diabetes. In children born to deficient mothers, increased rates of childhood asthma, type 1 diabetes, low birthweight, and autism spectrum disorder (ASD) have been observed. Although there is much evidence to support these correlations, much is yet to be understood regarding the etiology of these outcomes. This paper specifically examines the relationships between risk factors …

Cache Valley Resident Exposure To Pm2.5, Kristina Krepinski

Undergraduate Honors Capstone Projects

Air pollution poses one of the largest environmental risks to human health, and greatly contributes to increased mortality within populations. Of the different types of pollutants, fine particulate matter (PM_2.5) has the most adverse health effects. Long-term exposure to PM_2.5 is known to have serious health outcomes; however, evidence has indicated that even short-term exposure to moderate concentrations of PM_2.5 is detrimental to human health. While PM_2.5 does contribute to various respiratory conditions by affecting lung function, it also significantly affects the cardiovascular system. Elevated PM_2.5 exposure increases risk for cardiovascular disease, congestive heart …

Dietary Supplementation With Tart Cherries For Prevention Of Inflammation-Associated Colorectal Cancer In Mice, Ashlie Hunter

Undergraduate Honors Capstone Projects

The cherry fruit is a nutrient-dense food with comparatively low caloric content and significant amounts of key nutrients and bioactive food chemicals. Much of the health benefit of cherries is attributed to their high amounts of anthocyanins, which have anti-oxidant and anticancer properties that contribute to changes in cell signaling pathways involved in inflammation, carcinogenesis and angiogenesis. In this project, we aimed to determine whether dietary supplementation with tart cherries prevents colon tumor development in mice consuming a Western diet compared to a prudent diet. Previously, our research team developed the Total Western Diet (TWD) for mice to emulate typical …

The Effects Of Hazing On Urban Coyotes, Dakota Reed

Undergraduate Honors Capstone Projects

Urban expansion has rapidly increased over the last few decades. Over half of the human population now lives in urban areas (United Nations 2014). This has resulted in many carnivores being forced to adapt and find ways to use urban resources to survive (Bateman and Fleming 2012). While larger carnivores usually end up locally extinct, some medium sized predators like red fox (Vulpes vulpes), coyotes (Canis latrans), and raccoons (Procyon lotor) thrive in urban environments (Bateman and Fleming 2012). Having these carnivores living among humans can have both positive and negative consequences for the …

Relationships Of Beef Cattle Temperament With Feedlot Performance, Jeffrey James Briscoe

Undergraduate Honors Capstone Projects

This study determined relationships between beef cattle temperament and their feedlot performance. Thirty-five Angus cross-bred steers and heifers (18 steers and 17 heifers) were placed in the Utah State University feedlot to take part in the feed trial. Cattle temperament was measured by a chute score (CS, ranging from 1 = calm and not moving to 5 = rearing and violently struggling) and flight speed. Flight speed was measured by two observers to determine how long the animal took to travel 12 feet from the exit of the squeeze chute. Feedlot performance was assessed by body weight (BW) measurements, average …

The Role Of Orexin Receptors In Diurnal Variations In Learning And Memory, Jacob Christian Blotter

Undergraduate Honors Capstone Projects

The brain's ability to learn and remember is a topic of extensive debate and research. Mammals share many similarities, including the way in which information from the outside world is processed and stored. Mammalian circadian rhythms have long been thought to be essentially involved with these processes. Orexin, a neurotransmitter in the brain, plays a crucial role in arousal and circadian rhythm. This study is designed to explore the brain's ability to process and store information at different times of the circadian period, and to explore the role of orexin by comparing the performance of normal (wild-type) mice and abnormal …

Factors Of Profitable Field Crop Selection, Jacob Thomas Briscoe

Undergraduate Honors Capstone Projects

Methods of crop selection are varied, depending on a variety of factors, including resources, climate, soil type, and potential marketability of the crop. This study utilizes a theoretical farm of one thousand farmable acres to estimate the costs and returns as well as the resources associated with cultivating, planting, irrigating, harvesting and selling the crop products. The theoretical farm is situated in southwest Idaho in any of the counties of Ada, Canyon, Elmore, Owyhee and Payette. The crops grown are typical of the area; the crops examined are field corn (Zea mays), alfalfa (Medicago sativa) and …

Spa47 Is An Oligomerization - Activated Type Three Secretion System (T3ss) Atpase From Shigella Flexneri, Jamie Lee Kingsford

Undergraduate Honors Capstone Projects

Gram-negative pathogens often use conserved type three secretion systems (T3SS) for virulence. The Shigella type three secretion apparatus (T3SA) penetrates the host cell membrane and provides a unidirectional conduit for injection of effectors into host cells. The protein Spa4 7 localizes to the base of the apparatus and is speculated to be an ATPase that provides the energy for T3SA formation and secretion. Here, we developed an expression and purification protocol, producing active Spa47 and providing the first direct evidence that Spa47 is a bona fide ATPase. Additionally, size exclusion chromatography and analytical ultracentrifugation identified multiple oligomeric species of Spa47 …

The Influence Of Prior Motor Actions On Motor Planning In Children With Autism Spectrum Disorder, Daisha Lynn Cummins

Undergraduate Honors Capstone Projects

Studies have found children with Autism Spectrum Disorder (ASD) have a rigidity of motor plans and difficulties planning and executing movements. Children with ASD also exhibit repetitive behaviors such as nail biting and rocking back and forth. When planning for movements, repetitive behaviors might manifest as difficulty in formulating new or switching between different motor plans. In typically developing individuals, actions are often influence by recently performed motor actions. We sought to determine if this influence was stronger in children with ASD.

A rotation motor task was created to evaluate the rigidity of motor planning of five children with ASD …

Using Accelerometer Data To Remotely Assess Predation Activity Of Arctic Wolves, Heather Shipp

Undergraduate Honors Capstone Projects

Arctic wolves (Canis lupus arctos) play an important role in ecosystems located in the far northern regions of the world; however, unlike the gray wolves in Yellowstone National Park, little information is available about High Arctic wolves and their impacts on prey populations. This research uses data received from two GPS radio-collared Arctic wolves located in the Fosheim Peninsula on Ellesmere Island. Each radio-collar was programmed to record a position every 30-60 minutes, as well as the wolfs activity movement (forwards - backwards and left - right), which was generated by an accelerometer housed within the radio-collar. This …

The Effect Of Pure Infrared Light On The Growth Of Rhodospirrilum Rubrum, Jordan Lee Wilkes

Undergraduate Honors Capstone Projects

Scientists who study aquatic ecosystems quickly notice a diversity of pathways that different microbes and organisms can use to metabolize nutrients found in common ponds or pools. Competition for vital resources, such as light and inorganic minerals, allow only certain organisms to grow in certain niches within these ecosystems. Rhodospirillum rubrum is a gram negative, photosynthetic bacteria that competes for light within aquatic ecosystems in order to survive. R. rubrum is believed to specifically absorb light for photosynthesis at wavelengths in the range of infrared light. It was found that R. rubrum indeed can grow in "dark", anaerobic environments by …

Non-Suitable Habitat A Cause For Declining Bobolink Populations In Northern Utah, Bethany Q. Unger

Undergraduate Honors Capstone Projects

Bobolink, Dolichonyx oryxivories, populations are declining in Utah. I characterized the habitat conditions of known bobolink nesting sites in Utah and compared these conditions to those for nest sites in Wisconsin where bobolinks are abundant. My habitat assessment included identifying vegetation species, vegetation cover, pH, temperature, and precipitation at each site location. Vegetation cover different between Utah and Wisconsin nest sites. Precipitation varied for both locations with no correlation between water availability and bobolink presence. One possible driver for the reduction in bobolinks throughout Utah is the drastic increase in temperature. Other possible external factors include livestock grazing, edge distance, …

Effect Of Aggregation At A Winter Feeding Station On Intestinal Parasite Load In Elk (Cervus Canadensis), Morgan Jaromilla Hughes

Undergraduate Honors Capstone Projects

Winter feeding stations are used throughout the western US to reduce elk depredation of crops and haystacks on private lands. Many of the unintended effects of such artificial congregation remain unexamined, but generally, across species, locally increased host densities result in increased parasite loads. This adds physiological stress to individual animals and in game species such as elk it could reduce their value to sportsmen. Through laboratory analyses of fresh samples, we recorded nematode egg densities in elk feces collected during two periods ( early and late) in the supplementary feeding season. Mean nematode egg density remained fairly constant in …

Dna Methylation Analysis Of Lin28a & Hand1 In Electrostimulated Genetically Unmodified Porcine Fibroblast Cells Grown In Vitro, Sara E. Calicchia

Undergraduate Honors Capstone Projects

Pluripotent (undifferentiated) cells are important for their applications in regenerative medicine. However, gene-based nuclear reprogramming of adult somatic cells is a slow and inefficient process, and poses some risk to recipient patients. Therefore, a major goal within the field of stem cell biology is to find a way to accomplish adult somatic cell de-differentiation using small chemical modulators of gene expression or other non-genomic mechanisms. Pilot studies in our laboratory have suggested that cells exposed to silver ions generated by the passage of a weak electrical current through a silver wire show morphological and gene expression changes reminiscent of de-differentiation. …

Developing A Portable System For Measuring Human Motor Learning, Karen Elizabeth Tew

Undergraduate Honors Capstone Projects

Point-to-point reaching is a commonly used paradigm in the field of human motor control. By studying how people move their arms from one location in space to another, researchers have gained insight into how the central nervous system controls and learns skilled movement. Many experimental methods that are designed to study reaching are not portable. This makes it difficult for researchers to access certain clinical populations with limited mobility or motor dysfunction. We have addressed this issue by developing a point-to-point reaching system that can capture key movement variables (e.g. speed and accuracy) yet is portable and inexpensive. We have …

Group Housing And Social Stress In Side-Blotched Lizards (Uta Stansburiana), Marilize Van Der Walt

Undergraduate Honors Capstone Projects

Stress in regards to an animal's social housing environment is well studied in mammals; however there are few studies examining this in reptiles and the results are thus far unclear. For example, behavioral evidence shows adverse effects from individual housing in juvenile chameleons, however physiological measures in whiptail lizards show adverse effects from group housing. Because some reptiles appear to be affected negatively by their social housing environment while others are impacted positively, if we can discover the reason for these differences, we will be able to implement the most effective housing regimes for reptiles. In this study, we tested …

Sources Of Uncertainty In Stream Nutrient Sampling Below A Point Source, Alexandria Hayden Campbell

Undergraduate Honors Capstone Projects

The goal of this study was to determine what aspects of sampling and sample storage could lead to uncertainty when taking samples in a stream below a point source. Sources of uncertainty studied were the locations where the samples were taken to assess if nutrients were adequately mixed within a cross-section, different filtration techniques, dilution errors, analytical uncertainty, and freezing time. Bootstrapping analyses were used to determine whether mixing and dilution errors led to uncertainty, while one-way ANOVAs were used to evaluate filtration techniques and storage time. Sample spikes to determine percent recovery of nutrients and repeat sample analyses are …

An Overview Of Batrachochytrium Dendrobatidis In Utah, With A Focus On Boreal Toads And Their Changing Conservation Status, Samantha A. Beirne

Undergraduate Honors Capstone Projects

The Boreal Toad (Anaxyrus boreas) has disappeared from a large portion of its range in southern Utah and it has been questioned whether Batrachochytrium dendrobatidis (Bd, also known as chytrid) has played a role in its disappearance. The role of chytrid in range contraction of Boreal Toads and other amphibians in Utah is unknown. The primary objective of this project is to determine if any historic Boreal Toad specimens have chytrid to determine its arrival in Utah. If any old specimens test positive, the secondary goal of this study is to determine if there is a relationship between …

Quantifying Non-Game Fish Sampling Biases And Demographics To Better Understand The Role Of Fish In Pelican Diet And Distribution At Strawberry Reservoir, Ut, Jamie Reynolds

Undergraduate Honors Capstone Projects

Predation and competition are natural ecological processes, though these interactions occasionally cause concern among humans when ecosystem services are involved (e.g., popular fisheries in highly managed systems). The population of American white pelican (Pelecanus erythrorhynchos) at Strawberry Reservoir (Utah) has increased dramatically in the last decade, as have the populations of Utah sucker (Catostomus ardens) and Utah chub (Gila atraria). Anglers and managers are concerned that predation by pelicans and competition from non-game fish species are negatively impacting the reservoir's prized Bonneville cutthroat trout (Oncorhynchus clarkii utah) fishery. My project focused on …

The Effects Of Zno Nanoparticles On Egg, Larva, And Adult Rough-Skinned Newts (Taricha Granulosa), Austin Reid Spence

Undergraduate Honors Capstone Projects

The objective of this study was to examine the effects of zinc oxide (ZnO) nanoparticles on egg, larva, and adult rough-skinned newts, Taricha granulosa. To date, little research has been done to investigate the potentially detrimental effects of nanoparticles on amphibians, especially salamanders and newts (caudates). Chronic toxicity was tested on eggs and larvae, and acute toxicity was tested on eggs, larvae, and adults. For eggs, chronic exposure to ZnO nanoparticles caused higher mortality at 10.0 and 100.0 mg/L compared to 0.0, 0.1, and 1.0 mg/L. When given an acute exposure (24h) to nanoparticles at a late incubation stage, …