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Full-Text Articles in Life Sciences
Cloning And Expression For The Future Characterization Of The Air2 Protein, Emily Sue Frampton
Cloning And Expression For The Future Characterization Of The Air2 Protein, Emily Sue Frampton
Undergraduate Honors Capstone Projects
Air2 is a eukaryotic protein involved in multiple biological processes including protein-protein interactions as well as RNA binding. Air2 plays a critical role in RNA quality control and also helps regulate post-translational modification of various proteins. Although previous studies have revealed information regarding Air2's roles within a cell, the molecular and structural basis for Air2 function is unclear. Using a codon-optimized version of the Air2 gene, various constructs were created that improved the expression and solubility of Air2. Additionally a co-expression complex of Air2 with a PRMTI mutant, K13S, was made to obtain the Air2 protein with a native binding …
Ability Of A Specific Nuclear Transport Protein Complexed To The Pegfp Reporter Gene To Increase The Efficiency Of Transgenic Blastocyst Production, Emily Swank
Undergraduate Honors Capstone Projects
In the production of transgenic animals, one of the most common methods employed is the microinjection of DNA into the pronucleus of the embryo. This method was used to produce the first transgenic animal, a mouse (Gordon et al., 1981). Five years later, the first transgenic farm animal experiments were conducted. These studies involved the insertion of genes into pigs and sheep to increase growth (Hammer et al., 1985). Since that time many other transgenic animals including rabbits, sheep, pigs, and cattle have been produced.
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Undergraduate Honors Capstone Projects
Because of the damaging effects of bluetongue disease on the livestock industry, interest exists in developing a means of inducing passive immunity in infected animals. Antibodies against VP2, the polypeptide responsible for immunoantigenicity in bluetongue virus (BTV), have been found to elicit neutralizing antibodies; however, production of this purified antigen is very costly and thus impractical for commercial purposes. Total viral protein, prepared by a simple, rapid, highly reproducible SDS/KCl method, was injected into rabbits and found to produce antibodies which are useful in immunoblots. However, plaque neutralization assays failed to detect any neutralization activity by these antibodies. Denaturation of …