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Full-Text Articles in Life Sciences
Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse
Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse
Undergraduate Honors Capstone Projects
Cloning is an important technique in functional genomics, particularly genetics and molecular biology. Cloned genes can be used to facilitate protein expression; this is an essential step in understanding gene function. In traditional cloning, the gene of interest is amplified and tagged with a restriction enzyme sequence and ligated to the digested open vector. However, the GATEWAY™ Cloning Technology provides a speedy and more efficient route to insert the gene of interest in the desired vector, known as Destination vectors or Expression vectors, using site-specific recombination sites and recombinase enzymes.1 Once DNA segments from the gene of interest are …
Cloning And Expression For The Future Characterization Of The Air2 Protein, Emily Sue Frampton
Cloning And Expression For The Future Characterization Of The Air2 Protein, Emily Sue Frampton
Undergraduate Honors Capstone Projects
Air2 is a eukaryotic protein involved in multiple biological processes including protein-protein interactions as well as RNA binding. Air2 plays a critical role in RNA quality control and also helps regulate post-translational modification of various proteins. Although previous studies have revealed information regarding Air2's roles within a cell, the molecular and structural basis for Air2 function is unclear. Using a codon-optimized version of the Air2 gene, various constructs were created that improved the expression and solubility of Air2. Additionally a co-expression complex of Air2 with a PRMTI mutant, K13S, was made to obtain the Air2 protein with a native binding …