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Full-Text Articles in Life Sciences

Properties Of Microbes In Natural Fire Burn Soils, Holly Anderson Aug 2010

Properties Of Microbes In Natural Fire Burn Soils, Holly Anderson

Undergraduate Honors Capstone Projects

Soil underlying a natural fire develops a hydrophobic soil sub-layer. This hydrophobicity decreases with time although the mechanisms are unresolved, but are thought to be biotic and abiotic. Some of the compounds accounting for hydrophobicity are polycyclic aromatic hydrocarbons (PAHs) of which pyrene is an example.

Bacteria that grew on pyrene were isolated from burned soils at two sites in Utah in order to analyze the biotic microbial degradation of the hydrophobic soil sub-layer. The two sites were Wood Camp (Logan, UT) and Milford Flats (Central UT). Identifications of the genera of nine isolated bacteria were made through l6S rRNA …


Acyl Homoserine Lactone Signaling In Pseudomonas Chlororaphis 06, Anna Hegsted May 2010

Acyl Homoserine Lactone Signaling In Pseudomonas Chlororaphis 06, Anna Hegsted

Undergraduate Honors Capstone Projects

Pseudomonas chlororaphis (PcO6), a rhizosphere bacterium, is important to agriculture because of its use as a biological control against fungus and other pests. This biological control correlates with production of phenazines; a process regulated by acyl homoserine lactones (AHSLs). Long chain AHSLs might also have an affect on the production of phenazines. My research showed that PcO6 produced long chain AHSLs and that production is affected by nutrition with more long chain AHSLs produced in rich medium than minimal medium. The nutritional studies and findings with different mutants of PcO6 altered in phenazine production suggest that control of these antifungal …


Genetic And Biochemical Studies Of Plasmid Pir52-1 In Lactobacillus Helveticus, Cody Alexander Tramp May 2010

Genetic And Biochemical Studies Of Plasmid Pir52-1 In Lactobacillus Helveticus, Cody Alexander Tramp

Undergraduate Honors Capstone Projects

Lactobacillus helveticus is a species of lactic acid bacteria. These bacteria, which produce lactic acid as a major product of carbohydrate metabolism, are used industrially to produce cheese and other fermented dairy products (Ebringer et al., 2008). Many species of lactic acid bacteria also possess probiotic characteristics and when ingested potentially confer increased immune function, regulate gut microbiota, and improve digestion in the host (Reid, 2008). Genetic studies of these probiotic effects and other characteristics of L. helveticus and related species have been hindered by the lack of stably replicating plasmid vectors. Plasmid vectors for several Lactobacillus species have been …


Lifespan Of Prokaryote Model Organism Escherichia Coli K-12, Hyrum Gillespie May 2010

Lifespan Of Prokaryote Model Organism Escherichia Coli K-12, Hyrum Gillespie

Undergraduate Honors Capstone Projects

Bacteria are amazingly resilient organisms, in that they have evolved and adapted to the many extreme environments of Earth (from salt, to pH, to temperature extremes etc.). The bacterial lifespan and death rates are as important as its growth rates in these extreme environments. Bacteria would be useful to determine the effects of age on single cells, but because bacteria reproduce asexually by binary cell fission (clonal replication), calculating the lifespan has proven elusive. Without the determination of a lifespan, age studies using bacteria have limited application. Further, it has been proposed that organisms whose somatic cell line is not …