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Full-Text Articles in Life Sciences
The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch
The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch
Undergraduate Honors Capstone Projects
Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Undergraduate Honors Capstone Projects
The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …
The Role Of Plant Hormones In Grass Tiller Development, Marit Snow
The Role Of Plant Hormones In Grass Tiller Development, Marit Snow
Undergraduate Honors Capstone Projects
Two bunchgrass species commonly found in the Intermountain West, Agropyron spicatum and A. desertorum, are morphologically similar but respond to defoliation very differently. A. desertorum produces more daughter tillers and is more hardy than A. spicatum. Hormones were applied to experimental plants to observe changes in tillering rates, growth rates, and response of plants to the stress of exogenous hormone application. Gibberellic acid treatments were related to increased tiller height in both species, but also a decrease in daughter tiller production in A. desertorum and an increase in senescence in A. spicatum. Kinetin increased senescence in A. desertorum. Auxin showed …