Life Sciences Commons^™

Characterization Of Neutrophil Extracellular Traps In Naked Mole-Rats: A Step Towards Cancer Resistance, Thomas Abraham Smith

Undergraduate Honors Capstone Projects

Cancer is the second leading cause of death in the United States, exceeded only by heart disease. One of every five deaths in the United States is due to cancer. A growing area of research involves the analysis of cancer resistant traits in other species to understand their biological mechanisms and eventually make translations to human cancer research and clinical treatment. Because of their remarkable cancer resistance, the naked mole-rat (NMR) is a prime subject for this research, and various studies have already suggested that the immune mechanisms of the NMR might be harnessed for human cancer therapies^1-4,7. …

Protein Nutrition And Immunity In Male Bumblebees (Bombus Impatiens), Carson Stoker

Undergraduate Honors Capstone Projects

Male bumble bees play a vital but understudied role in their colonies. They are essential to colony reproduction and success, despite not emerging until the end of the colony's life cycle. Even so, the biology of male bumble bees is not well understood, which leaves our understanding of colony health incomplete. This knowledge gap is important because bumblebee populations are declining, which threatens the ecosystems and industries which rely on them for pollination. This study aimed to understand how two understudied factors of bumble bee health—diet and immunity—are related in males. Pollen, a bee’s main source of protein, has been …

Applying The Extended Parallel Process Model To Climate Change Communication, Mikenna Renee Debruin

Undergraduate Honors Capstone Projects

Whereas over 99% of the scientific community believes in the concept of anthropogenic climate change, lay support is still lagging behind. I suggest three key factors to lagging lay support: 1) complexity, 2) manufactured scientific controversy, and 3) doom-and-gloom framing. Because of these factors, individuals are less willing believe in the prevalence of human-induced climate change, nonetheless do something about it. The Extended Parallel Process Model (EPPM), however, presents a model to assuage eco-anxiety and counter psychological distance from the problem. The EPPM, proposed by Kim Witte in the 1990s, combines appeals to threat and appeals to efficacy to contextualize …

Working Like A Dog: Literary Analysis Of Multifactorial Service Dog Outcomes., Sarah Brinck

Undergraduate Honors Capstone Projects

What makes a service dog successful? Whether it is genetics or the environment they are raised in, the definition of a successful service dog is complicated. Organizations that set out to train service dogs depend on successful outcomes to continue operating. That makes it particularly important to determine and understand the primary factors that impact the success or failure of dogs bred and/or trained for service dog programs. Given the complexity of the requirements for effective service dogs, it can be costly for an individual or an organization to neglect any factors that contribute to success. Individual components of what …

A Music Composition Through The Use Of Animal Sounds, Andy Nguy

Undergraduate Honors Capstone Projects

The knowledge on animals has been studied over many years by researching and understanding animal behavior and creativity with music. Bioacoustics shows a great deal when it comes to collecting sounds through many principles for sound data collection. With saved recording of animals, music, speeches, and so much more, it has impacted the way music is created through technology. Music production has been advancing in many creative ways. The foundation of sound manipulation is musique concrète. The project uses these concepts of audio recording and digital sounds to produce a composition that includes animal sounds.

Antimicrobial Assay Of Sagebrush Roots, Lauren Rachel (Moody) Mcfadden

Undergraduate Honors Capstone Projects

Plants produce secondary metabolites for various functions, one of which is antibacterial activities. Sagebrush has historically been used by Native Americans for different medicinal purposes, suggesting that it may have secondary metabolites that would have medicinal values, including antimicrobial activities, and can be a natural source for antibiotics. This study aims to carry out the antimicrobial activity of Sagebrush root extract against a handful of bacteria. We tested the antimicrobial activity of root extract of Sagebrush against six bacteria such as Bacillus subtilis, Bacillus cereus, E. coli DHSα (Lab cloning strain), Agrobacterium tumefaciens GV3101 (Lab cloning strain), Pseudomonas …

Estimating Cattle Density Using Wildlife Cameras, Emily Bonebrake

Undergraduate Honors Capstone Projects

Quantifying the abundance and distribution of animal populations is critical for effective wildlife research and management. Due to their cost-effectiveness, wildlife cameras have become an increasingly popular tool for estimating population densities. Previously, this technique relied on ‘capture-recapture’ models that utilized re-sightings of individually marked animals, but in recent years methods have been developed to estimate the population densities of unmarked animals. One such method is the random encounter and staying time (REST) technique, which does this by assuming that the cumulative time animals stay within the view of the camera scales linearly with the number of individuals. This allows …

Effect Of Basal Diet And Black Raspberry Supplementation On Gene Biomarkers Of "Leaky Gut" In A Mouse Model Of Colitis-Associated Colorectal Cancer, Eliza Catherine Stewart

Undergraduate Honors Capstone Projects

Colorectal cancer is currently the second leading cause of cancer death in the world. The risk of developing colorectal cancer increases significantly in individuals who suffer from colitis, which is inflammation of the colon lining, seen in Irritable Bowel Disease. The term “leaky gut” is often used to describe increased intestinal permeability and is closely related to colitis. Leaky gut is associated with dysbiosis of the gut microbiota, which can occur through diet. This dysbiosis leads to prolonged inflammation and affects genes that encode for proteins involved in the tight junctions between cells. Compromised intestinal integrity allows for translocation of …

A Study On The Impact Of Diet On Unified Fire Authority Firefighter Performance, Marin Easton

Undergraduate Honors Capstone Projects

Objective: This project aimed to examine the impact of nutrition knowledge, dietary intake, and physical activity on firefighters’ performance on their routine physical assessment (RPA).

Methodology: Members of the Unified Fire Authority, the largest fire agency in Utah with more than 400 sworn firefighters serving 15 communities representing 440,000 residents, were invited via email to participate in this cross-sectional study. Participants (N=92) completed a 40- question survey that assessed specialized diet adherence, dietary intake, nutrition knowledge (using questions from the General Nutrition Knowledge Questionnaire;1 point for each correct answer [26 points possible]), regular physical activity (International Physical Activity Questionnaire), and …

Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse

Undergraduate Honors Capstone Projects

Cloning is an important technique in functional genomics, particularly genetics and molecular biology. Cloned genes can be used to facilitate protein expression; this is an essential step in understanding gene function. In traditional cloning, the gene of interest is amplified and tagged with a restriction enzyme sequence and ligated to the digested open vector. However, the GATEWAY™ Cloning Technology provides a speedy and more efficient route to insert the gene of interest in the desired vector, known as Destination vectors or Expression vectors, using site-specific recombination sites and recombinase enzymes.¹ Once DNA segments from the gene of interest are …

Utah Tick Surveillance An Animated Public Service Announcement, Keith Wilson

Undergraduate Honors Capstone Projects

As the United States’ population grows, we develop more land into housing and recreate farther into wilderness areas, consequentially encountering ticks with increasing frequency. As the climate continues to change, tick population distributions are also changing, influencing our population’s exposure to tick-borne diseases. Lyme disease, a tick-borne disease named after Lyme, Connecticut, is one of the fastest growing emerging diseases in North America, and the most prevalent vector-borne infection in the United States. There are two species of tick in North America, Ixodes scapularis and Ixodes pacificus, known to be carriers of the causative agent of Lyme disease, a …

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preference, Hailey Hatch

Undergraduate Honors Capstone Projects

Floral microbes are an overlooked aspect of the extended floral phenotype. Through altering floral nectar chemistry, they can mediate interactions between flowers, pollinators, and other floral microbes, with significant implications for plant and pollinator health. Interactions between floral microbes and pollinators are critically important to understand, as pollinators provide important ecosystem services in both natural and agriculture systems. Here, I explored how floral nectar traits affected both evolution and competition within the floral yeast Metschnikowia reukaufii, the floral bacterium Bacillus subtilis, and other microbes isolated from Brassica rapa nectar, an important plant model system and oilseed crop. To …

The Evolution And Unraveling Of The American Eugenics Movement, Anneka Walton

Undergraduate Honors Capstone Projects

While the evolution of American eugenics surrounding the time of Nazi Germany has been well-documented, the periods before the explosion of American eugenics and the result of the movement in the wake of the Holocaust are not so well known. Many historically-based works have described the path and growth of eugenics from the time the term was coined to the public acknowledgement of World War II and the point where Americans ended eugenic euthanasia and slowed eugenic sterilizations. There are also many works within the realm of disability history that document these events from the more common social viewpoint. This …

Environmental Racism In A Growing City: Investigating Demographic Shifts In Salt Lake City's Polluted Neighborhoods, Emma Nathel Jones

Undergraduate Honors Capstone Projects

Studies investigating the spatial distribution of environmental hazards have repeatedly demonstrated the existence of environmental racism -- the disproportionate impact of environmental hazards on communities of color. We aim to contribute to research on environmental racism by asking how relationships between race and hazard exposure change over time. Our study area, Salt Lake City (SLC), UT, USA is one of the largest cities in the intermountain west and is expected to see continued population growth. SLC was 99% white from 1860-1950. 2019 census estimates indicate that SLC is becoming more racially diverse with 35.6% of the population identifying as racial …

Effects Of Sex And Autism On Oxytocin Receptors In The Substantia Nigra Of The Human Brain, Kip Dooley

Undergraduate Honors Capstone Projects

Oxytocin, a hormone present in the mammalian brain, has been shown to be a vital component of social function in animals and may have a role in the social deficits associated with Autism Spectrum Disorder in humans. Based on previous studies from our lab, there are oxytocin receptors in the human substantia nigra, a basal ganglia structure in the midbrain that is important in both movement and reward pathways. The substantia nigra contains two subsections that are defined by the neurotransmitters they contain: the pars compacta, which is dopaminergic, and the pars reticulata, which is GABAergic. By localizing oxytocin receptors …

Choice Of End State Comfort Based On Time Spent At The Beginning State And The Precision Requirement Of The End State, Tucker Gregory Gamble

Undergraduate Honors Capstone Projects

People choose actions based on many different variables. In particular, choice of posture while grasping an object typically depends upon several factors including the time spent in that posture, what postures were held prior to choosing that posture, and the precision required by the posture. The purpose of this study was to test a trade-off between choice of end-state comfort based on time spent in a posture at the beginning-state and the precision requirement of the end-state. A comfortable grasp is classified when a person has a full grasp on an object with their thumb pointing up. This posture also …

Autism & Autoimmunity, Clarissa Nelson

Undergraduate Honors Capstone Projects

My research was for the Honors Capstone, and consisted of familiarizing myself with the vast amount of research in the fields of autism and autoimmunity and how the two may be connected, then going on to design a new project to help acquire more information where these two fields overlap. The official abstract I have written for this new project goes as follows: Our proposed research project is focused primarily in the fields of autism and autoimmunity, and hopes to uncover more evidence that these two fields are related. We believe that autism is an autoimmune disease, and our project …

Emergent Seedling Spatial Patterns Following Insect Seed Predation In A Simulated Population, Justin Tirrell

Undergraduate Honors Capstone Projects

Tropical vertebrate species have faced increasing pressures from hunters, causing many to become locally extinct. I used an agent-based model (NEDD) to investigate the influence of dispersal and insect seed predation on seedling survival. Statistical dispersal kernels were used to simulate the dispersal of seeds. The NEDD model generates survival and spatial data from parameter sets, which were chosen based on a Latin-Hypercube experimental design. Spatial point analysis was performed on the output data to identify trends in spatial clustering patterns as the parameter space was changed. The results of this investigation suggest that there is a positive association between …

Intra- And Interspecific Secondary Metabolite Variation Between Fruit And Leaf Tissues In The Hyperdiverse Psychotria Genus, Cole A. Carlson

Undergraduate Honors Capstone Projects

Secondary metabolites are chemical compounds that are considered to mediate a variety of plant interactions with their environment and are not involved in basic metabolism. Recently, there has been an interest in understanding the function and allocation of these metabolites in fruit tissues. In contrast to leaves, the chemistry in fruit tissue mediates exclusive interactions with seed dispersers that directly affect plant fitness and are under different evolutionary selective pressures. Only a few studies outline the patterns of chemistry between fruit and leaf tissues. This study aims to understand how secondary metabolites in two species of the hyperdiverse congeneric genus …

Exposure Of Phlebotomus Argentipes To Alpha-Cypermethrin, Permethrin, And Ddt Using Cdc Bottle Bioassays To Assess Insecticide Susceptibility, Jacob Rex Andersen

Undergraduate Honors Capstone Projects

Background: Insecticide resistance for sand flies is a concern since sand flies are vectors for Leishmania spp. parasites which cause leishmaniasis affecting millions of people each year. The CDC bottle bioassay is used to assess resistance by comparing known insecticide diagnostic doses and diagnostic times from an insecticide-susceptible population. The objective of this study was to determine diagnostic doses and diagnostic times for α-cypermethrin and the lethal dose for 50% and 90% mortality for α-cypermethrin, permethrin, and DDT for Phlebotomus argentipes.

Methods: The CDC bottle bioassays were performed in 1,000 mL glass bottles with 15-25 sand flies from a laboratory …

The Response Of Male Bumblebees To A Putative Queen Pheromone, Xavier K. Haemmerle

Undergraduate Honors Capstone Projects

Queen pheromones are chemical signals produced by the dominant reproductive female in many species of eusocial insects. These pheromones are vital for maintaining a reproductive division of labor. Two evolutionary scenarios may describe the origin of queen pheromones. Sensory exploitation describes a scenario where the pheromone is produced to take advantage of a preexisting sensory bias in a population. An alternative scenario is that the recipient of the pheromone has an adapted response to a preexisting chemical signal. There is a growing body of evidence that cuticular hydrocarbons that act as queen pheromones are co-opted from ancient fertility signals that …

Determining The Need For Meal Preparation Education In First-Year University Students, Alicia Kunzler

Undergraduate Honors Capstone Projects

Background: In previous research, college students reported low confidence and varying skill in meal preparation ability. This study evaluates first-year university students to determine the acceptability of meal preparation programming for this population.

Objective: To assess university freshmen's skill level, confidence, and interest in food budgeting, meal planning, and cooking techniques to determine potential interventions.

Methods: First-year university students (n=265; 58 men, 205 women) were recruited through Facebook and email invitations to complete a 50-item survey. Survey categories included skills/confidence in food budgeting (8), meal planning (12), and cooking techniques 12); class interest (4), student background information (9), and general …

Effects Of Reproductive Maturity On Neuroplasticity In Male Bumble Bees (Bombus Impatiens), Karlee Eck

Undergraduate Honors Capstone Projects

Within the span of an insect's lifetime, new synaptic connections between neurons are made in response to age and experience. This neuroplasticity leads to anatomical changes within individual brain regions that represent investment in different brain functions. The neuroplasticity of female bumble bees has been well studied, but almost nothing is known about how the male bee brain responds to life experiences. Due to the complex sensory requirements of mate finding and copulation for males, neuroplasticity is likely to be an important component of reproduction. These sensory requirements include olfactory and optic inputs, which are processed in the calyces of …

Young Germ Cell Depleted Ovaries In Post-Reproductive Mice And Its Effects On Immune Function, Mckenna Walters

Undergraduate Honors Capstone Projects

It has previously been shown that young, cycling ovarian transplantation in aged female mice increased the general health and life span in regard to their post-reproductive health. It has further been hypothesized that this enhancement of health is directly influenced by the ovarian somatic cells. To address this hypothesis, transplants of young germ cell depleted and germ cell containing ovaries were performed on female mice. The purpose of this study is to continue to discern the reproductive influence on aging health, specifically in the area of immunological well-being. Control group mice were separated by age and treatment mice were subsequently …

Analyzing Fear Free Veterinary Practices And Their Effectiveness In Managing Client And Patient Fear, Anxiety, And Stress, And The Profitability Of These Techniques, Heather Hamblin

Undergraduate Honors Capstone Projects

Fear-Free veterinary techniques are a new approach to caring for patients and clients in the veterinary clinic that have gained a great deal of popularity and traction. However, many veterinary professionals are left to question whether the utilization of these practices is resulting in a big enough change in client and patient stress levels to make it worth implementing these methods. This research looks into the views of veterinarians and clients on the effectiveness of these practices, and the competitive advantage of having a fear-free certified clinic from a business point of view.

The Effect Of A Surfactant Seed Coating On The Germination And Growth Of Three Native Bulrushes, Anders Hart

Undergraduate Honors Capstone Projects

Great Salt Lake (GSL) wetlands provide vital ecosystem services, including habitat for migratory birds. Alkali bulrush (Bolboschoenus maritimus), hardstem bulrush (Schoenoplectus acutus), and three-square bulrush (Schoenoplectus americanus) play an important role in providing these services, but invasion by Phragmites australis has reduced the extent these species in GSL wetlands. Restoring these native bulrushes following Phragmites removal is a primary goal for GSL managers. However, climate change and increasing human water demands upstream may alter the hydropattem of GSL wetlands, leading to lower soil moisture availability and potentially inhibiting germination and establishment of these species. …

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Lactic-Acid Producing Bacterial Strains To Identify Synbiotic Combinations, Michaela Brubaker

Undergraduate Honors Capstone Projects

Dietary interventions with probiotic lactic acid bacteria (LAB) and prebiotics, complex dietary fibers that promote LAB growth, may favorably shift the gut microbiome to reduce colorectal cancer risk. Our primary hypothesis was that the LAB strains NCFM Lactobacillus acidophilus and HNO 19 Bifidobacterium lactis would thrive in the presence of agave, green banana, black raspberry, baobab fruit, or pomegranate peel whole food powders by fermenting their oligosaccharide (OS) components into lactic acid end products. LAB strains were cultured in media with no carbohydrate, purified OS, or one of the whole food powders. LAB strains cultured with agave appeared to grow …

Percussive Behavior In The Southern Resident Killer Whale (Orcinus Orca) Population At Lime Kiln Point State Park, Rylee Jensen

Undergraduate Honors Capstone Projects

The endangered Southern Resident Killer Whale (Orcinus orca, SRKW) is an iconic species in the Pacific Northwest. Although many ecological aspects of this population have been studied, including population dynamics, genealogy, diet, and habitat-use patterns, why SRKW perform above-surface "percussive" behavior such as breaching, cartwheeling, pectoral fin slapping, tail lobbing, and dorsal fin slapping remains unclear. In the present study, a) individual percussive behavioral data was recorded during the summer of 2016 to evaluate trends by age and sex class, and b) an existing long-term data set on SRKW was analyzed to compare the relationship between the seasonal …

Evaluating The Potential Of Repurposing Commercially Available Drugs For The Treatment Of Viral Infections, Brennan Connor Mcewan

Undergraduate Honors Capstone Projects

Viral infections that are often overlooked as common seasonal illnesses such as influenza can rapidly become a public threat. They threaten society as new, more dangerous strains of these common viruses emerge and as strains develop resistance to current vaccines and antiviral treatments (Kochanek, Murphy, Xu, & Tejada-Vera, 2014). To combat this, the development of antiviral treatments with novel mechanisms of action is essential. Repurposing drugs instead of developing new drugs can save years of development time and hundreds of millions of dollars (DiMasi, Hansen, & Grabowski, 2003). To support the effort to discover drugs with unique mechanisms of action, …

Manipulation Of Ovarian Function Significantly Influenced Glucose Metabolism In Cba/J Mice, Kyleigh Ann Tyler

Undergraduate Honors Capstone Projects

Menopause is associated with a decline in overall health in women. One health aspect impacted is glucose metabolism. As women experience menopause, their metabolism declines dramatically. The current study addressed the influence of ovarian somatic cells on the improvement of metabolic health through transplantations of young, germ cell-depleted ovaries. The purpose of this study is to expand the understanding of female reproductive health on metabolism. Control mice were grouped by age and treatment mice were age-matched. Treatment mice were placed into one of three groups: 1) mice received germ cell-depleted ovaries, 2) mice received germ cell-containing ovaries, and 3) mice …