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Articles 691 - 720 of 2496

Full-Text Articles in Life Sciences

Effects Of Silicon And Beneficial Bacteria On Sheath Blight Of Rice And The Microbial Community Of Rice Rhizosphere, Jhonson Leonard Jul 2019

Effects Of Silicon And Beneficial Bacteria On Sheath Blight Of Rice And The Microbial Community Of Rice Rhizosphere, Jhonson Leonard

LSU Master's Theses

Sheath blight (ShB), caused by Rhizoctonia solani Kühn, is an economically important soil-borne disease of rice (Oryza sativa) that can cause up to 50 % yield losses. Application of fungicides is the most effective and commonly used method for managing sheath blight. However, the pathogen can develop new resistant strains upon continuous exposure to a fungicide. Moreover, the use of inorganic fungicides is not eco-friendly. The utilization biological control agents (BCAs) can be a more sustainable and safe means of managing ShB. To develop alternate strategies for ShB management, I am exploring the effect of the silicon (Si) …

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Agricultural Education Teachers' Perceptions And Use Of Environmental Education In Louisiana Schools, Olivia M. Soler Jul 2019

Agricultural Education Teachers' Perceptions And Use Of Environmental Education In Louisiana Schools, Olivia M. Soler

LSU Master's Theses

The purpose of this study was to describe the status of environmental education (EE) in Louisiana high school agriculture classrooms. A census of Louisiana Agriscience Teacher Association (LATA) members was conducted to understand their perceptions, attitudes, and barriers regarding EE implementation. Icek Ajzen’s Theory of Planned Behavior was utilized as the theoretical framework. Data were collected through an online survey research method and analyzed using descriptive statistics. Results suggest that most LATA members incorporate EE into their curriculum relatively sparingly, perceive EE to be beneficial for students, and are in need of funding to incorporate EE into their curriculum as …

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Temporal And Spatial Dynamics Of Competitive Parapatry In Chewing Lice, Mark S. Hafner Jul 2019

Temporal And Spatial Dynamics Of Competitive Parapatry In Chewing Lice, Mark S. Hafner

Faculty Publications

We synthesize observations from 1979 to 2016 of a contact zone involving two subspecies of pocket gophers (Thomomys bottae connectens and T. b. opulentus) and their respective chewing lice (Geomydoecus aurei and G. centralis) along the Rio Grande Valley in New Mexico, U.S.A., to test predictions about the dynamics of the zone. Historically, the natural flood cycle of the Rio Grande prevented contact between the two subspecies of pocket gophers. Flood control measures completed in the 1930s permitted contact, thus establishing the hybrid zone between the pocket gophers and the contact zone between their lice (without hybridization). Since that time, …

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Louisiana Agriculture Summer, 2019, Lsu Agcenter Jul 2019

Louisiana Agriculture Summer, 2019, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

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Development, Validation, And Testing Of The Eating And Food Literacy Behaviors Questionnaire With Young Adult University Students, Kwadernica Rhea Jul 2019

Development, Validation, And Testing Of The Eating And Food Literacy Behaviors Questionnaire With Young Adult University Students, Kwadernica Rhea

LSU Doctoral Dissertations

The Eating and Food Literacy Behaviors Questionnaire (EFLBQ) was developed, and its validity, reliability, and ability to measure construct changes was established. Items related to the originally proposed domains of food literacy were included in the initial questionnaire, however five, new components were discovered. The second version of the EFLBQ was tested with 257 young adult university students using exploratory factor analysis, and a five-factor model (R2=57.4%) was returned. Cronbach’s alpha coefficients were calculated (health and nutrition=0.89, taste=0.72, food preparation=0.77, planning /decision-making=0.64, and convenience=0.63). A confirmatory factor analysis, with 923 new responses provided evidence of an adequate fit …

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Effects Of Virus Infection And Volatiles On Aphid Virus Vector Behavior On Sweetpotato, John Lawrence Dryburgh Jul 2019

Effects Of Virus Infection And Volatiles On Aphid Virus Vector Behavior On Sweetpotato, John Lawrence Dryburgh

LSU Doctoral Dissertations

Sweetpotato is affected by a wide variety of viruses worldwide, which can cause yield losses of up to 90%. Many of these viruses are transmitted by aphids in a non-persistent manner. Non-persistent viruses are acquired and transmitted within minutes, and thus conventional control, such as insecticides, are ineffective. Altering aphid movement and feeding behavior may affect the rate of virus transmission. One potential method to alter aphid behavior is volatile organic compounds (VOC), including volatiles emitted by virus infected plants, plant hormones, and commercial control agents. Aphid movement near sweetpotato fields was monitored to determine trends throughout the growing season. …

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In Vitro And In Vivo (Mouse Model) Investigation Of The Efficacy Of Probiotic Bacteria Lactobacillus Casei And Lactobacillus Rhamnosus Incorporated In Tomato Juice, Bennett Atta Dzandu Jul 2019

In Vitro And In Vivo (Mouse Model) Investigation Of The Efficacy Of Probiotic Bacteria Lactobacillus Casei And Lactobacillus Rhamnosus Incorporated In Tomato Juice, Bennett Atta Dzandu

LSU Doctoral Dissertations

Fruit and vegetable production in Ghana were estimated at 371,811 tons in 2017 with tomatoes ranked among the top four most cultivated vegetables. The majority of the production goes to waste due to spoilage and lack of processing. Probiotics have recently gained much attention owing to claims about their potential health benefits. However, the inability of certain probiotics to survive gastric conditions remain a potential setback. Tomatoes can be processed into value added products including those fortified with probiotics. Such value-added products can be used as protectant for the delivery of probiotics to improve gut health. The objective of this …

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Iron-Sulfur Cluster Biosynthesis In Methanogens, Cuiping Zhao Jun 2019

Iron-Sulfur Cluster Biosynthesis In Methanogens, Cuiping Zhao

LSU Doctoral Dissertations

Methanogens live in a syntrophic consortium with bacteria, taking advantage of the metabolic abilities of their syntrophic partners to overcome energetic barriers and break down compounds that they cannot digest by themselves. Interspecies electron transfer, which is a major type of microbial communication in syntrophic processes, improves methanogenesis and anaerobic oxidization of methane (AOM) processes involved in syntrophic consortia. These processes have a significant impact on the global carbon cycle. Most of the essential enzymes involved in methanogenesis are iron-sulfur proteins. Iron-sulfur clusters are one of the oldest and most versatile cofactors present in all domains of life. To date, …

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Characterization Of The Integrated Signaling Network Of Burkholderia Glumae For The Regulation Of Virulence-Related Function In The Bacterial Pathogenesis Of Rice Plants, Tiago De Paula Lelis Jun 2019

Characterization Of The Integrated Signaling Network Of Burkholderia Glumae For The Regulation Of Virulence-Related Function In The Bacterial Pathogenesis Of Rice Plants, Tiago De Paula Lelis

LSU Doctoral Dissertations

Bacterial panicle blight of rice (BPB) caused by Burkholderia glumaeis a major bacterial disease of rice in many rice-producing areas worldwide. Managing the disease is still very difficult due to the lack of effective methods. B. glumaestrains show phenotypic and genomic variations; however, little variation was detected in the known virulence-related genes. In the present study, the complete genome of B. glumae257sh-1, a natural avirulent strain, was sequenced and analyzed. Several spontaneous mutations in the regulatory genes of these avirulent strains were identified. Presumably, the accumulation of these mutations can have a negative effect on the normal …

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The Role Of Atp-Sensitive Inward Rectifier Potassium Channels In The Regulation Of Reactive Oxygen Species In The Western Honey Bee, Apis Mellifera L., Christopher J. Fellows Jun 2019

The Role Of Atp-Sensitive Inward Rectifier Potassium Channels In The Regulation Of Reactive Oxygen Species In The Western Honey Bee, Apis Mellifera L., Christopher J. Fellows

LSU Master's Theses

Colonies of managed honey bees are annually being lost at an unsustainable rate, partly due to reduced immunocompetence that leads to acute viral outbreaks and mortality. To aid in restoring honey bee health despite the myriad of environmental stressors, this thesis focuses on identifying novel physiological pathways that can mitigate virus-mediated mortality through increased immune function. Previous work has demonstrated that a family of potassium ion channels, termed KATP channels, mediate the survival of honey bees during infection from a model virus, suggesting KATP channels may drive antiviral immunity. Interestingly, these channels have been linked to the regulation …

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Cogongrass (Imperata Cylindrica (L.) Beauv.) In Louisiana: Cause And Consequence, Lorissa A. Radunzel-Davis Jun 2019

Cogongrass (Imperata Cylindrica (L.) Beauv.) In Louisiana: Cause And Consequence, Lorissa A. Radunzel-Davis

LSU Master's Theses

Cogongrass (Imperata cylindrica (L.) Beauv.), an invasive species from East Asia, is found worldwide and is problematic in several countries. In the United States, it grows primarily in the Southeast, reducing biodiversity by growing in dense patches and potentially causing mortality and reducing value of native and planted pinelands due to a high burning temperature. Using Lee Memorial Forest, a Louisiana State University AgCenter property in Washington Parish as a study site, this thesis explores cogongrass in Louisiana with emphasis on soil microbes and soil legacy effects on native plant species. Cogongrass populations at Lee Memorial Forest were more …

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Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sa Jun 2019

Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sa

LSU Doctoral Dissertations

The Janus Kinase - Signal Transducer and Activator of Transcription (JAK-STAT) signaling pathway transduces several signals crucial for development and homeostasis. Suppressors of cytokine signaling (SOCS) proteins control JAK-STAT signaling via a negative feedback loop. The transcription factor STAT5 is known to play a significant role in fat cell development and function, and several studies suggest that acetylation may affect STAT5 transcriptional activity. To test this hypothesis, we treated 3T3-L1 adipocytes with growth hormone (GH) to activate STAT5 in the presence or absence of histone deacetylase (HDAC) inhibitors. STAT5 acetylation levels were low in adipocytes and mostly unchanged by the …

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In Vitro Evaluation Of Ovarian Cancer Tumorigenesis As A Function Of Quinone Oxidoreducatse-1 And Cell Phenotype, Milcah S. Jackson Jun 2019

In Vitro Evaluation Of Ovarian Cancer Tumorigenesis As A Function Of Quinone Oxidoreducatse-1 And Cell Phenotype, Milcah S. Jackson

LSU Doctoral Dissertations

In vitro multicellular spheroids are attractive model systems for assessing genetic and epigenetic changes that occur in diseased tissues. Understanding how such alterations in gene and subsequent protein expression affect disease progression and metastasis, drug resistance, and recurrence is of great interest in cancer research. In this regard, examining expression and activity of proteins, such as those with cytoprotective ability that are overexpressed in cancer cells, in addition to cell phenotype (i.e., stem-like, epithelial, mesenchymal, or mixed), are two ways to evaluate genetic and epigenetic changes. Moreover, determining the impact that cytoprotective proteins and cell phenotype have on tumor formation …

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Spatiotemporal Impact Of Snow On Underwater Photosynthetically Active Radiation In Taylor Valley, East Antarctica, Madeline E. Myers Jun 2019

Spatiotemporal Impact Of Snow On Underwater Photosynthetically Active Radiation In Taylor Valley, East Antarctica, Madeline E. Myers

LSU Master's Theses

The role of snow on underwater photosynthetically active radiation (UW PAR) in the McMurdo Dry Valleys (MDVs) has been understudied due to lack of a detailed snowfall record. Research has shown that a relationship between snow cover and UW PAR exists, but the extent has never been evaluated in great detail. Although annual snowfall values in the MDVs are low (3 to 50 mm water equivalent annually), trends of increasing snowfall on the continent under future warming conditions could lead to an increased role for snow in regulating UW PAR (and associated primary productivity). Here, I discuss evidence from the …

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Matrix Recharge In A Shrink-Swell Floodplain Forest Soil, Savannah R. Morales Jun 2019

Matrix Recharge In A Shrink-Swell Floodplain Forest Soil, Savannah R. Morales

LSU Master's Theses

Despite the global distribution of fine-grained Vertisols, the hydrology of these floodplain soils is still not well understood. Vertisols shrink and swell depending on soil moisture leading to a range of soil pore sizes, from large macropore cracks to smaller micropores, and consequently a range of hydraulic conductivities. Despite the plethora of research indicating the importance of both flooding and soil moisture in floodplain ecosystems, the specific role that flooding plays in soil moisture recharge has been less widely studied and remains of interest. Blue food dye and deuterated water were used as tracers to determine the role of the …

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High-Performance Computing Frameworks For Large-Scale Genome Assembly, Sayan Goswami Jun 2019

High-Performance Computing Frameworks For Large-Scale Genome Assembly, Sayan Goswami

LSU Doctoral Dissertations

Genome sequencing technology has witnessed tremendous progress in terms of throughput and cost per base pair, resulting in an explosion in the size of data. Typical de Bruijn graph-based assembly tools demand a lot of processing power and memory and cannot assemble big datasets unless running on a scaled-up server with terabytes of RAMs or scaled-out cluster with several dozens of nodes. In the first part of this work, we present a distributed next-generation sequence (NGS) assembler called Lazer, that achieves both scalability and memory efficiency by using partitioned de Bruijn graphs. By enhancing the memory-to-disk swapping and reducing the …

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Influence Of Hydration Status On Running Performance And Makers Of Psychological And Physiological Stress In High School Cross Country Runners, Joshua Granger Jun 2019

Influence Of Hydration Status On Running Performance And Makers Of Psychological And Physiological Stress In High School Cross Country Runners, Joshua Granger

LSU Master's Theses

Adequate hydration is an important attribute to an individual’s health and wellbeing. This is highlighted in athletic populations, who are at great risk for hypohydration, regardless of age, sex, and sport. The rampant hypohydration observed in athletes is present at all levels of competition, from professional athletes, with access to substantial monetary and nutrition support to division 3 and recreational athletes who often face monetary challenges. This trend continues to trickle down to lower levels of competition, such as high school, where athletes often practice and compete in dehydrated states. Practicing in these hypohydrated states can cause health issues such …

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Maximizing And Modeling Malonyl-Coa Production In Escherichia Coli, Tatiana Thompson Silveira Mello Jun 2019

Maximizing And Modeling Malonyl-Coa Production In Escherichia Coli, Tatiana Thompson Silveira Mello

LSU Master's Theses

In E. coli, fatty acid synthesis is catalyzed by the enzyme acetyl-CoA carboxylase (ACC), which converts acetyl-CoA into malonyl-CoA. Malonyl-CoA is a major building block for numerous of bioproducts. Multiple parameters regulate the homeostatic cellular concentration of malonyl-CoA, keeping it at a very low level. Understanding how these parameters affect the bacterial production of malonyl-CoA is fundamental to maximizing it and its bioproducts. To this end, competing pathways consuming malonyl-CoA can be eliminated, and optimal nutritional and environmental conditions can be provided to the fermentation broth. Most previous studies utilized genetic modifications, expensive consumables, and high-cost quantification methods, making …

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The Respiratory Burst Oxidase Homolog D (Rbohd) Cell And Tissue Distribution In Potato-Potato Virus Y (Pvyntn) Hypersensitive And Susceptible Reactions, Rodrigo A. Valverde Jun 2019

The Respiratory Burst Oxidase Homolog D (Rbohd) Cell And Tissue Distribution In Potato-Potato Virus Y (Pvyntn) Hypersensitive And Susceptible Reactions, Rodrigo A. Valverde

Faculty Publications

The respiratory burst oxidase homolog D (RbohD) acts as a central driving force of reactive oxygen species signaling in plant cells by integrating many different signal transduction pathways in plants, including incompatible interactions with pathogens. This study demonstrated the localization and distribution of RbohD in two types of potato-potato virus Y (PVY) interactions: Compatible and incompatible (resistant). The results indicated a statistically significant induction of the RbohD antigen signal in both interaction types. In the hypersensitive response (resistant reaction) of potato with a high level of resistance to the potato tuber necrotic strain of PVY (PVYNTN), RbohD localization followed by …

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The Use Of Geospatial Modeling And Novel Diagnostics To Detect And Map Risk Factors Of Soil-Transmitted Helminths In Feira De Santana, Brazil, Ryan Harry Avery May 2019

The Use Of Geospatial Modeling And Novel Diagnostics To Detect And Map Risk Factors Of Soil-Transmitted Helminths In Feira De Santana, Brazil, Ryan Harry Avery

LSU Doctoral Dissertations

Soil-transmitted helminth (STH) infections impact billions of people worldwide. The traditional STH control approach is a morbidity control strategy implementing mass drug administration (MDA) programs targeting school-aged children (SAC). In Brazil, this control strategy has decreased STH prevalence to less than 20% in most of the country and providing an opportunity to transition from the morbidity control program and towards a surveillance and response system geared towards STH elimination. Surveillance and response systems geared towards elimination require the implementation higher accuracy diagnostics to detect infection in low-transmission communities, surveillance of entire households, high-resolution modeling at the household-habitat scale, and targeted …

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Sodium Reduction Through Salt Substitute Mixtures, Visual Cues, And Salt Level Statements, And Its Effects On Consumer Perception: A Case Of Barbecue Sauce, Jose Ramon Alonso Marenco May 2019

Sodium Reduction Through Salt Substitute Mixtures, Visual Cues, And Salt Level Statements, And Its Effects On Consumer Perception: A Case Of Barbecue Sauce, Jose Ramon Alonso Marenco

LSU Doctoral Dissertations

Cardiovascular diseases are the leading causes of death in the USA and associated with excessive sodium (Na) intake. Thus, reducing the Na intake is vital to alleviate health concerns. One serving of the average Barbecue sauce (BBQs) may contribute around 15%-20% of the daily Na allowance (2,300 mg). No research has evaluated salt substitutes or visual cues on salty taste perception of BBQs, thus it was the aim of this research. Consumer studies followed a Balanced Incomplete Block (BIB) designs. For Study I, BBQs prototypes followed a 3-components (NaCl : KCl : Glycine) mixture design, with 38% - 87% less …

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A Case-Study Approach To Investigate Transmission, Co-Infection, And Clinical Sequelae During Epidemics Of Dengue And Ebola Virus Disease, Jennifer Elizabeth Giovanni May 2019

A Case-Study Approach To Investigate Transmission, Co-Infection, And Clinical Sequelae During Epidemics Of Dengue And Ebola Virus Disease, Jennifer Elizabeth Giovanni

LSU Doctoral Dissertations

From within their ecologic niches, zoonotic viruses emerge from animal reservoirs into the edges and centers of human habitation to exploit opportunities for unabated transmission within immunologically–naïve populations. Our understanding of where, in whom, and how these viruses emerge is under direct challenge, driving the evolution of modern infectious disease epidemiology within a rapidly-connected global community. The studies presented herein are based on analyses of both aggregate and case-level data, which, we argue, provide unique insight into the complexities of transmission, co-infection, and clinical sequelae occurring within, and arising from, epidemics of emerging zoonotic viruses. In Chapter II, we investigate …

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Role Of The Drosophila Beaf Protein In Chromatin Domain Insulator And Promoter Function, Mukesh Maharjan May 2019

Role Of The Drosophila Beaf Protein In Chromatin Domain Insulator And Promoter Function, Mukesh Maharjan

LSU Doctoral Dissertations

Proper folding of eukaryotic genomes is required to allow correct interactions between different parts of chromosomes. Precise and timely interactions among different parts of a chromosome allow proper functioning inside a nucleus, including gene regulation, DNA replication and DNA repair. Eukaryotic regulatory elements that facilitate folding and interactions include enhancers, promoters and insulator elements. Insulator elements and their binding proteins play an important role in regulating correct chromatin structure and function. The Drosophila melanogaster special chromatin structure (scs’) is one such insulator. The Boundary Element Associated Factor (BEAF) binds to scs’. BEAF is a 32 kDa protein that has two …

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Physiological Characterization Of Inward Rectifier Potassium (Kir) Channels In Insect Neural Systems, Rui Chen May 2019

Physiological Characterization Of Inward Rectifier Potassium (Kir) Channels In Insect Neural Systems, Rui Chen

LSU Master's Theses

A complete understanding of the physiological pathways critical for proper function of the insect nervous system is still lacking. The recent development of potent and selective small-molecule modulators of insect inward rectifier potassium channels has enabled the interrogation of the physiological role and toxicological potential of Kir channels within various insect tissue systems. Therefore, we aimed to highlight the physiological and functional role of neural Kir channels in the central nervous system, muscular system, and neuromuscular system through pharmacological and genetic manipulations. Our data provide significant evidence that Drosophila neural systems rely on the inward conductance of K+ ions for …

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Hsv-1 Live-Attenuated Vaccine Strain Vc2 Confers Complete Protection In Mice Against Ocular Immunipathogenesis By The Human Clinical Strain Hsv-1 (Mckrae), Shan Krishnan Naidu May 2019

Hsv-1 Live-Attenuated Vaccine Strain Vc2 Confers Complete Protection In Mice Against Ocular Immunipathogenesis By The Human Clinical Strain Hsv-1 (Mckrae), Shan Krishnan Naidu

LSU Doctoral Dissertations

Herpes simplex virus type-1 (HSV-1) can cause severe ocular infection and blindness. We have previously shown that the HSV-1 VC2 vaccine strain is protective in mice and guinea pigs against genital herpes infection following vaginal challenge with HSV-1 or HSV-2. In this study, we evaluated the efficacy of VC2 intramuscular vaccination in mice against herpetic keratitis following ocular challenge with the lethal human clinical strain HSV-1(McKrae). VC2 vaccination in mice produced superior protection and morbidity control in comparison to its parental strain HSV-1(F). Specifically, after HSV-1(McKrae) ocular challenge, all VC2- vaccinated mice survived, while 30% of the HSV-1(F)- vaccinated and …

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Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata May 2019

Antimicrobial Properties Of Different Chitosans Against Gram-Negative And Gram-Positive Foodborne Pathogens In Food Products, Nancy Katherine Rubio Zapata

LSU Doctoral Dissertations

Chitosan is a polymer derived of the deacetylation of chitin that is one of the most abundant material in nature. Chitosan solutions applied as edible coatings, and dipping solutions have shown positive results in the extension of shelf life on seafood and meat products. The purpose of this study was to evaluate the antimicrobial activities of a newly invented high molecular weight water-soluble (HMWWS) chitosan against selected Gram-negative (Salmonella Typhimurium, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae) and Gram-positive (Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) foodborne pathogens (initial inoculation of 6.5 Log). Chitosans with …

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Improving The Food Safety Of Louisiana Strawberry Industry, Jose L. Brandao Delgado May 2019

Improving The Food Safety Of Louisiana Strawberry Industry, Jose L. Brandao Delgado

LSU Doctoral Dissertations

Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes have been linked to foodborne outbreaks in produce. The most recent outbreaks in produce have been associated with irrigation water due to infiltration of well water or water run-off from contaminated sources. The Food Safety Modernization Act (FSMA) requires all irrigation water to be safe for use on produce, as a strategy to reduce foodborne illnesses. A surfactant modified zeolite (SMZ) filtration system could provide produce farmers with a sustainable low-cost system for high-quality and safe irrigation water. The objective of this study was to evaluate the effectiveness of hexadecyltrimethylammonium …

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Changes In Salivary Antimicrobial Protein Concentrations In Response To Maximal Exercise In Collegiate Swimmers, Katelyn Hardin May 2019

Changes In Salivary Antimicrobial Protein Concentrations In Response To Maximal Exercise In Collegiate Swimmers, Katelyn Hardin

Honors Capstones

No abstract provided.

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Identification Of Blood Biomarkers Of Mild Traumatic Brain Injury In Collegiate Football Players, Amy Turner May 2019

Identification Of Blood Biomarkers Of Mild Traumatic Brain Injury In Collegiate Football Players, Amy Turner

Honors Capstones

No abstract provided.

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Zinc Toxicity And Iron-Sulfur Cluster Biogenesis In Escherichia Coli, Huangen Ding May 2019

Zinc Toxicity And Iron-Sulfur Cluster Biogenesis In Escherichia Coli, Huangen Ding

Faculty Publications

While zinc is an essential trace metal in biology, excess zinc is toxic to organisms. Previous studies have shown that zinc toxicity is associated with disruption of the (4Fe-4S] clusters in various dehydratases in Escherichia coli. Here, we report that the intracellular zinc overload in E. coli cells inhibits iron-sulfur cluster biogenesis without affecting the preassembled iron-sulfur clusters in proteins. Among the housekeeping iron-sulfur cluster assembly proteins encoded by the gene cluster iscSUA-hscBA-fdx-iscX in E. coli cells, the scaffold IscU, the iron chaperone IscA, and ferredoxin have strong zinc binding activity in cells, suggesting that intracellular zinc overload inhibits iron-sulfur …

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