Life Sciences Commons^™

Crop Updates 2006 - Lupins And Pulses, Peter White, Mark Seymour, Amelia Mclarty, Wayne Parker, Ian Pritchard, Bob French, Rodger Beermier, Bevan Buirchell, Mark Sweetingham, Jon Clements, Geoff Thomas, Roger Jones, Sofia Sipsas, John Quealy, Leigh Smith, Gordon Francis, Huaan Yang, Jeffrey Boersma, Margaret Pallotta, Chengdao Li, Krishnapillai Sivasithamparam, M. Nelson, H. Phan, S. Ellwood, P, Moolhuijzen, M. Bellgard, J. Hane, A. Williams, J. Fos-Nyarko, B. Wolko, M. Książkiewicz, M. Cakir, M. Jones, M. Scobie, C. O'Lone, S. J. Barker, R. Oliver, W. Cowling, Laurie Maiolo, Martin Harries, Jo Walker, Murray Blyth, Stephen Cosh, Dirranie Kirby, Kedar Adhikari, Patrizia Guantini, Katie Bell, Ken Adcock, Ciara Beard, Anne Smith, Tim Maling, Art Diggle, Debbie Thackray, Kadambot Siddique, Ping Si, Huaan Yang, Vijay Jayasena, Florence Kartawinata, Ranil Coorey, Ken Dods, Kerry Regan, Tanveer Khan, Jenny Garlinge, Rod Hunter, Pam Burges, Eric Armstrong, Glen Riethmuller, Greg Shea, Moin Salam, Jean Galloway, Pip Payne, Bill Macleod, Brenda Coutts, Pooran Gaur, Heather Clarke, Stuart Morgan, Alan Harris, Ted Knights, Kristy Hobson, Chris Matthews, Colin Hanbury, Tim Pope, Mike Baker, Leanne Young, Syed Muhammad Nasar-Abbas, Julie Plummer, D. Harris, Phill Chambers, Michael Materne, Dennis Van Gool, Huyen Phan, Simon Ellwood, J. Hane, Angela Williams, R. Ford, S. Thomas, R. Oliver, Rory Coffey, Chris Poole, Theo Pfaff, Judith Lichtenzveig, Lars Kamphuis, Nola D'Souza, Emma Groves, Karam Singh

Crop Updates

This session covers sixty six papers from different authors:

2005 LUPIN AND PULSE INDUSTRY HIGHLIGHTS

1. Lupin Peter White, Department of Agriculture

2. Pulses Mark Seymour, Department of Agriculture

3. Monthly rainfall at experimental sites in 2005

4. Acknowledgements Amelia McLarty EDITOR

5. Contributors

6. Background Peter White, Department of Agriculture

2005 REGIONAL ROUNDUP

7. Northern agricultural region Wayne Parker, Department of Agriculture

8. Central agricultural region Ian Pritchard and Bob French, Department of Agriculture

9. Great southern and lakes Rodger Beermier, Department of Agriculture

10. South east region Mark Seymour, Department of …

Crop Updates 2006 - Farming Systems, Wayne Pluske, Bill Bowden, Craig Scanlan, Stephen Davies, Chris Gazey, Peter Tozer, Derk Bakker, Louise Barton, David Gatter, Renee Buck, Daniel Murphy, Christoph Hinz, Bill Porter, Meredith Fairbanks, Nicolyn Short, Ian Foster, James Fisher, Doug Abrecht, Mario D'Antuono, Tracey M. Gianatti, Paul Carmody, Frank D'Amden, Rick Llewellyn, Michael Burton, Caroline Peek, Nadine Eva, Chris Carter, Megan Abrahams, Andrew Blake, Paul Blackwell, Sylvian Pottier, Michael Robertson, Greg Lyle, Lisa Brennan, Tony J. Vyn, Simon Teakle, Peter Norris, Jeff Russell, James Fisher, Roy Murray-Prior, Deb Pritchard, Mike Collins, Greg Hamilton, Rob Hetherington, Andrew Van Burgel, Cliff Spann

Crop Updates

This session covers nineteen papers from different authors:

SOIL AND NUTRIENT MANAGEMENT

1. Inve$tigating fertili$er inve$tment, Wayne Pluske, Nutrient Management Systems

2. KASM, the potassium in Agricultural System Model,Bill Bowden and Craig Scanlan, DAWA Northam and UWA, School of Earth and Geographical Sciences

3. Long term productivity and economic benefits of subsurface acidity management from surface and subsurface liming, Stephen Davies, Chris Gazey and Peter Tozer, Department of Agriculture

4. Furrow and ridges to prevent waterlogging, Dr Derk Bakker, Department of Agriculture

5. Nitrous oxide emissions from a cropped soil in Western Australia, Louise Barton^{1 …}

Crop Updates 2006 - Oilseeds, Graham Walton, Fiona Martin, Anne Wilkins, Nathan Hancock, Matthew Nelson, Marieclaire Castello, Linda Thompson, Anouska Cousin, Guijun Yan, Wallace Cowling, Moin Salam, Bill Mcloud, Ravjit Khangura, Jean Galloway, Art Diggle, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Terry Rose, Qifu Ma, Dave Eksteen

Crop Updates

This session covers thirteen papers from different authors:

1. INTRODUCTION, Graham Walton, CONVENOR, Department of Agriculture

2. The performance of new TT canola varieties in National Variety Testing (NVT) WA, Fiona Martin, Research Agronomist, Agritech Crop Research

3. Comparison of TT Canola Varieties in Oilseeds WA Trials – 2005, Collated by G.H. Walton, Department of Agriculture, WA, from a collaboration between Oilseeds WA, Seed Companies, Agronomists and Growers

4. An overview of the potential for a Biofuels Industry in Western Australia, Anne Wilkins and Nathan Hancock, Department of Agriculture

5. Retrieval of fertile progeny from interspecific …

Crop Updates 2006 - Cereals, Roger Jones, Nichole Burges, Mohommad Amjad, Ben Curtis, Wal Anderson, Darren Chitty, Brianna Peake, Harm Van Rees, Cherie Reilly, James Hunt, Dean Holzworth, Zvi Hochman, Allan Peake, Neal Dalgliesh, Stephen Van Rees, Trudy Mccann, Peter Carberry, Raj Malik, Kelly Winfield, Brenda Shackley, Judith Devenish, Darshan Sharma, Steve Penny Jr, Christine Zaicou-Kunesch, Mike Bolland, Ross Brennan, Paul Damon, Zed Rengel, Jeremy Lemon, Perry Poulton, Nick Poole, Brooke Thompson, Stephen Loss, Jeff Russell, Angie Roe, James Eyres, Narelle Simpson, Ron Mctaggart, Lionel Martin, Dave Allen, Hossein Golzar, Manisha Shankar, Robert Loughman, John Majewski, Donna Foster, Jamie Piotrowski, Nicole Harry, Geoff Thomas, Kith Jayasena, Svetlana Micic, Paul Matson, Tony Dore, Thomas (Ben) Biddulph, Daryl Mares, Julie Plummer, Tim Setter, Garren Knell, Steve Curtin, Wade Longmuir, Nigel Metz, Tina Botwright Acuña, Len Wade, Cindy Mills, Peter Stone

Crop Updates

This session covers twenty nine papers from different authors:

PLENARY

1. The 2005 wheat streak mosaic virus epidemic in New South Wales and the threat posed to the Western Australian wheat industry, Roger Jones and Nichole Burges, Department of Agriculture

SOUTH COAST AGRONOMY

2. South coast wheat variety trial results and best options for 2006, Mohammad Amjad, Ben Curtis and Wal Anderson, Department of Agriculture

3. Dual purpose winter wheats to improve productivity, Mohammad Amjad and Ben Curtis, Department of Agriculture

4. South coast large-scale premium wheat variety trials, Mohammad Amjad and Ben Curtis, Department of Agriculture …

Chlorella Virus Mt325 Encodes Water And Potassium Channels That Interact Synergistically, Sabrina Gazzarrini, Ming Kang, Svetlana Epimashko, James L. Van Etten, Jack Dainty, Gerhard Thiel, Anna Moroni

James Van Etten Publications

Fast and selective transport of water through cell membranes is facilitated by water channels. Water channels belonging to the major intrinsic proteins (MIPs) family have been found in all three domains of life, Archaea, Bacteria, and Eukarya. Here we show that Chlorella virus MT325 has a water channel gene, aqpv1, that forms a functional aquaglyceroporin in oocytes. aqpv1 is transcribed during infection together with MT325 kcv, a gene encoding a previously undescribed type of viral potassium channel. Coexpression of AQPV1 and MT325-Kcv in Xenopus oocytes synergistically increases water transport, suggesting a possible concerted action of the two channels in …

Farnesol-Induced Apoptosis In Aspergillus Nidulans Reveals A Possible Mechanism For Antagonistic Interactions Between Fungi, Camile P. Semighini, Jacob M. Hornby, Raluca Dumitru, Kenneth W. Nickerson, Steven D. Harris

Department of Plant Pathology: Faculty Publications

The dimorphic fungus Candida albicans secretes farnesol, which acts as a quorum-sensing molecule and prevents the yeast to mycelium conversion. In this study we examined the effect of farnesol in the filamentous fungus Aspergillus nidulans. We show that externally added farnesol has no effect on hyphal morphogenesis; instead, it triggers morphological features characteristic of apoptosis. Additional experiments suggest that mitochondria and reactive oxygen species (ROS) participate in farnesol-induced apoptosis. Moreover, the effects of farnesol appear to be mediated by the FadA heterotrimeric G protein complex. Because A. nidulans does not secrete detectable amounts of farnesol, we propose that it …

Registration Of Twelve Grain Sorghum Genetic Stocks Near-Isogenic For The Brown Midrib Genes Bmr-6 And Bmr-12, Jeffrey F. Pedersen, Deanna L. Funnell, John J. Toy, A.L. Oliver, R.J. Grant

Department of Plant Pathology: Faculty Publications

Twelve grain sorghum [Sorghum bicolor (L.) Moench] genetic stocks, N599 to N610, (Reg. no. GS-128–GS-139, PI 639709–PI 639720) near-isogenic to their wild-type counterparts for the brown midrib genes bmr-6 and bmr-12 were developed jointly by the USDA-ARS, and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and were released in May 2005.

Registration Of ‘Atlas Bmr-12’ Forage Sorghum, Jeffrey F. Pedersen, Deanna L. Funnell, John J. Toy, A.L. Oliver, R.J. Grant

Department of Plant Pathology: Faculty Publications

Atlas bmr-12 forage sorghum [Sorghum bicolor (L.)Moench] (Reg, no. CV-136, PI 636763) was developed jointly by the USDA, ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and was released in January 2005.

Registration Of Seven Forage Sorghum Genetic Stocks Near-Isogenic For The Brown Midrib Genes Bmr-6 And Bmr-12, Jeffrey F. Pedersen, Deanna L. Funnell, John J. Toy, A.L. Oliver, R.J. Grant

Department of Plant Pathology: Faculty Publications

Seven forage sorghum [Sorghum bicolor (L.) Moench] genetic stocks, N592 to N598, (Reg. no. GS-121–GS-127, PI639702– PI639708) near-isogenic to their wild-type counterparts for the brown midrib genes bmr-6 and bmr-12 were developed jointly by the USDA-ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and were released in January 2005.

Chlorella Viruses, Takashi Yamada, Hideki Onimatsu, James L. Van Etten

James Van Etten Publications

Chlorella viruses or chloroviruses are large, icosahedral, plaque‐forming, double‐stranded‐DNA— containing viruses that replicate in certain strains of the unicellular green alga Chlorella. DNA sequence analysis of the 330‐kbp genome of Paramecium bursaria chlorella virus 1 (PBCV‐1), the prototype of this virus family (Phycodnaviridae), predict ∼366 protein‐encoding genes and 11 tRNA genes. The predicted gene products of ∼50% of these genes resemble proteins of known function, including many that are completely unexpected for a virus. In addition, the chlorella viruses have several features and encode many gene products that distinguish them from most viruses. These products include: (1) multiple …

Carbon-To-Nitrogen Ratio And Carbon Loading Of Production Media Influence Freeze-Drying Survival And Biocontrol Efficacy Of Cryptococcus Nodaensis Oh 182.9, Shouan Zhang, David A. Schisler, Michael J. Boehm, Patricia J. Slininger

Department of Plant Pathology: Faculty Publications

Fusarium head blight (FHB), caused by Gibberella zeae, is a devastating disease of wheat worldwide. Cryptococcus nodaensis OH 182.9 is an effective biocontrol agent for this disease. Development of a dried product of OH 182.9 would have potential advantages of ease of handling, favorable economics, and acceptance by end users. Isolate OH 182.9 was grown for 48 and 72 h in semi-defined complete liquid (SDCL) medium with carbon-to-nitrogen (C/N) ratios of 6.5:1, 9:1, 11:1, 15:1, and 30:1, and in SDCL C/N 30:1 media with varied carbon loadings of 7, 14, 21, and 28 g/liter. Total biomass production and cell …

A Highly Efficient Transient Protoplast System For Analyzing Defence Gene Expression And Protein–Protein Interactions In Rice, Songbiao Chen, Lizen Tao, Lirong Zeng, Miguel E. Vega-Zanchez, Kenji Umemura, Guo-Liang Wang

Center for Plant Science Innovation: Faculty Publications

The transient assay system based on mesophyll or cultured cell-derived protoplasts has been exploited in several plant species and has become a powerful tool for rapid gene functional analysis and biochemical manipulations. However, the system has not been widely used in rice owing to the difficulties in large-scale isolation of viable rice protoplasts from leaves or suspension-cultured cells. Here, we describe a significantly improved method to isolate a large number of protoplasts from stem and sheath tissues of both young and mature plants. High-level coexpression of multiple constructs and efficient suppression of exogenous and endogenous genes were observed in the …

First Report Of Sudden Death Syndrome Of Soybean Caused By Fusarium Solani F. Sp. Glycines In Nebraska, A. D. Ziems, L. J. Giesler, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

During August of 2004, soybean (Glycine max (L.) Merr.) plants exhibiting symptoms typical of sudden death syndrome (SDS) caused by Fusarium solani (Mart.) Sacc. f. sp. glycines (= Fusarium virguliforme Akoi, O’Donnell, Homma, & Lattanzi) (1) were observed in Nemaha and Pierce counties in eastern Nebraska. Leaf symptoms ranged from small chlorotic spots to prominent interveinal necrosis on plants at R5-R6 growth stages. Taproots of symptomatic plants were plated on potato dextrose agar (PDA) amended with hymexazol, ampicillin, and rifampicin (HAR).

Influence Of Lysobacter Enzymogenes Strain C3 On Nematodes, J. Chen, W. H. Moore, Gary Yuen, D. Kobayashi, E.P. Caswell-Chen

Department of Plant Pathology: Faculty Publications

Chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. Here, the influence of Lysobacter enzymogenes strain C3 on Caenorhabditis elegans, Heterodera schachtii, Meloidogyne javanica, Pratylenchus penetrans, and Aphelenchoides fragariae is described. Exposure of C. elegans to L. enzymogenes strain C3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched juveniles after 2 d. Hatch of H. schachtii eggs was about 50% on a lawn of L. enzymogenes strain C3 on agar as compared to 80% on a lawn of E. coli …

First Report Of Sudden Death Syndrome Of Soybean Caused By Fusarium Solani F. Sp. Glycines In Nebraska, A. D. Ziems, L. J. Giesler, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

During August of 2004, soybean (Glycine max (L.) Merr.) plants exhibiting symptoms typical of sudden death syndrome (SDS) caused by Fusarium solani (Mart.) Sacc. f. sp. glycines (= Fusarium virguliforme Akoi, O'Donnell, Homma, & Lattanzi) (1) were observed in Nemaha and Pierce counties in eastern Nebraska. Leaf symptoms ranged from small chlorotic spots to prominent interveinal necrosis on plants at R5-R6 growth stages. Taproots of symptomatic plants were plated on potato dextrose agar (PDA) amended with hymexazol, ampicillin, and rifampicin (HAR). Resulting fungal isolates grew slowly and developed masses of blue macroconidia, characteristic of F. solani f. sp. glycines …

Avenacin Production In Creeping Bentgrass (Agrostis Stolonifera) And Its Influence On The Host Range Of Gaeumannomyces Graminis, S. L. Thomas, P. Bonello, P. E. Lipps, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Avenacinase activity has been shown to be a key factor determining the host range of Gaeumannomyces graminis on oats (Avena sativa). G. graminis var. avenae produces avenacinase, which detoxifies the oat root saponin avenacin, enabling it to infect oats. G. graminis var. tritici does not produce avenacinase and is unable to infect oats. G. graminis var. avenae is also reported to incite take-all patch on creeping bentgrass (Agrostis stolonifera). It is unknown whether creeping bentgrass produces avenacin and if the avenacin-avenacinase interaction influences G. graminis pathogenicity on creeping bentgrass. The root extracts of six creeping bentgrass …

Fungicide Sensitivity Of Sclerotinia Homoeocarpa From Golf Courses In Ohio, Young-Ki Jo, Amy L. Niver, Joseph W. Rimelspach, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Managing dollar spot, the most common and chronic disease on intensively cultivated turfgrass, relies on the judicious use of fungicides. The heavy use of fungicides has led to the development of isolates of Sclerotinia homoeocarpa insensitive to several classes of fungicides, including benzimidazoles, demethylation-inhibitors, and dicarboximides. In vitro fungicide sensitivity assays using single discriminatory concentrations of thiophanate-methyl, propiconazole, and iprodione were developed in this study for evaluating field efficacy of these fungicides and the prevalence of fungicide insensitivity within S. homoeocarpa isolated from golf courses throughout Ohio. Discriminatory concentrations for these fungicides were determined to be: thiophanate-methyl = 1,000 μg …

Divergence And Mosaicism Among Virulent Soil Phages Of The Burkholderia Cepacia Complex, Elizabeth J. Summer, Carlos F. Gonzalez, Morgan Bomer, Thomas Carlile, Addie Embry, Amalie M. Kucherka, Jonte Lee, Leslie Mebane, William C. Morrison, Louise Mark, Maria D. King, John J. Lipuma, Anne K. Vidaver, Ry Young

Department of Plant Pathology: Faculty Publications

We have determined the genomic sequences of four virulent myophages, Bcep1, Bcep43, BcepB1A, and Bcep781, whose hosts are soil isolates of the Burkholderia cepacia complex. Despite temporal and spatial separations between initial isolations, three of the phages (Bcep1, Bcep43, and Bcep781, designated the Bcep781 group) exhibit 87% to 99% sequence identity to one another and most coding region differences are due to synonymous nucleotide substitutions, a hallmark of neutral genetic drift. Phage BcepB1A has a very different genome organization but is clearly a mosaic with respect to many of the genes of the Bcep781 group, as is a defective prophage …

Assessing Heterodera Glycines-Resistant And Susceptible Cultivar Yield Response, P. A. Donald, P. E. Pierson, S. K. St. Martin, P. R. Sellers, G. R. Noel, A. E. Macguidwin, J. Faghihi, V. R. Ferris, C. R. Grau, D. J. Jardine, H, Melakeberhan, T. L. Niblack, W. C. Stienstra, G. L. Tylka, T. A. Wheeler, D. S. Wysong

Department of Plant Pathology: Faculty Publications

The soybean cyst nematode Heterodera glycines (SCN) is of major economic importance and widely distributed throughout soybean production regions of the United States where different maturity groups with the same sources of SCN resistance are grown. The objective of this study was to assess SCN-resistant and -susceptible soybean yield responses in infested soils across the northcentral region. In 1994 and 1995, eight SCN-resistant and eight SCN-susceptible public soybean cultivars representing maturity groups (MG) I to IV were planted in 63 fields, either infested or noninfested, in 10 states in the north-central United States. Soil samples were taken to determine initial …

Sentinel Nematodes Of Land-Use Change And Restoration In Tallgrass Prairie, T. C. Todd, T. O. Powers, P.G. Mullin

Department of Plant Pathology: Faculty Publications

Changes in land use and the associated changes in land cover are recognized as the most important component of human-induced global change. Much attention has been focused on deforestation, but grasslands are among the most endangered ecosystems on Earth. The North American tallgrass prairie is a dramatic example, exhibiting a greater than 95% decline in historical area. Renewed interest in prairie conservation and restoration has highlighted the need for ecological indicators of disturbance and recovery in native systems, including the below ground component. The tallgrass prairie differs from the agricultural systems that have replaced it in having greater diversity and …

First Report Of Leaf Spot Of Smooth Bromegrass Caused By Pithomyces Chartarum In Nebraska, C. Eken, C. C. Jochum, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

Smooth bromegrass (Bromus inermis Leyss.) is the most common perennial grass species cultivated for forage in North America. During late fall of 2004, smooth bromegrass plants in Lincoln, NE were observed to have brown lesions on leaf midveins that were several centimeters long. Symptomatic leaves were surface disinfested for 1 min in 2% NaOCl and incubated at 25°C on potato dextrose agar (PDA) and water agar. The fungus, Pithomyces chartarum (Berk. & Curt) Ellis, was isolated consistently and identified on the basis of morphological characteristics (1). Colonies were effused and black on PDA. Conidiophores measured 3.5 to 8 …

Bioinformatics-Enabled Identification Of The Hrpl Regulon And Type Iii Secretion System Effector Proteins Of Pseudomonas Syringae Pv. Phaseolicola 1448a, Monica Vencato, Fang Tian, James R. Alfano, C. Robin Buell, Samuel Cartinhour, Genevieve A. Declerck, David S. Guttman, John Stavrinides, Vinita Joardar, Magdalen Lindeberg, Philip A. Bronstein, John W. Mansfield, Christopher R. Myers, Alan Collmer, David J. Schneider

Department of Plant Pathology: Faculty Publications

The ability of Pseudomonas syringae pv. phaseolicola to cause halo blight of bean is dependent on its ability to translocate effector proteins into host cells via the hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To identify genes encoding type III effectors and other potential virulence factors that are regulated by the HrpL alternative sigma factor, we used a hidden Markov model, weight matrix model, and type III targeting-associated patterns to search the genome of P. syringae pv. phaseolicola 1448A, which recently was sequenced to completion. We identified 44 high-probability putative Hrp promoters upstream of genes encoding the …

Regulation Of Hyphal Morphogenesis And The Dna Damage Response By The Aspergillus Nidulans Atm Homolog Atma, Iran Malavazi, Camile P. Semighini, Marcia Regina Von Zeska Kress, Steven D. Harris, Gustavo H. Goldman

Department of Plant Pathology: Faculty Publications

Ataxia telangiectasia (A-T) is an inherited disorder characterized by progressive loss of motor function and susceptibility to cancer. The most prominent clinical feature observed in A-T patients is the degeneration of Purkinje motor neurons. Numerous studies have emphasized the role of the affected gene product, ATM, in the regulation of the DNA damage response. However, in Purkinje cells, the bulk of ATM localizes to the cytoplasm and may play a role in vesicle trafficking. The nature of this function, and its involvement in the pathology underlying A-T, remain unknown. Here we characterize the homolog of ATM (AtmA) in the filamentous …

A Position Paper On The Electronic Publication Of Nematode Taxonomic Manuscripts, Eyualem Abebe, James G. Baldwin, Byron Adams, Duane Hope, Scott Gardner, Robin Huettel, Peter Mullin, Thomas O. Powers, Jyotsna Sharma, Weimin Ye, William K. Thomas

Department of Plant Pathology: Faculty Publications

Several nematode species have now attained ‘model organism’ status, yet there remain many niches in basic biological inquiry for which nematodes would be ideal model systems of study. However, furthering the model system approach is hindered by lack of information on nematode biodiversity. The shortage of taxonomic resources to inventory and characterize biodiversity hinders research programs in invasion biology, ecosystem functioning, conservation biology, and many others. The disproportion between numbers of species to be described and numbers of available taxonomic specialists is greater for Nematoda than for any other metazoan phylum. A partial solution to the taxonomic impediment is the …

Sequencing Of Aspergillus Nidulans And Comparative Analysis With A. Fumigatus And A. Oryzae, James E. Galagan, Sarah E. Calvo, Christina Cuomo, Li-Jun Ma, Jennifer R. Wortman, Serafim Batzoglou, Su-In Lee, Meray Baştürkmen, Christina C. Spevak, Vladimir Kapitonov, Jerzy Jurka, Claudio Scazzocchio, Mark Farman, Jonathan Butler, Seth Purcell, Steven D. Harris, Gerhard H. Braus, Oliver Draht, Silke Busch, Christophe D'Enfert, Christiane Bouchier, Gustavo H. Goldman, Deborah Bell-Pedersen, Sam Griffiths-Jones, John H. Doonan, Jaehyuk Yu, Kay Vienken, Arnab Pain, Michael Freitag, Eric U. Selker, David B. Archer, Miguel Á. Peñalva, Berl R. Oakley, Michelle Momany, Toshihiro Tanaka, Toshitaka Kumagai, Kiyoshi Asai, Masayuki Machida, William C. Nierman, David W. Denning, Mark Caddick, Michael Hynes, Mathieu Paoletti, Reinhard Fischer, Bruce Miller, Paul Dyer, Matthew S. Sachs, Stephen A. Osmani, Bruce W. Birren

Department of Plant Pathology: Faculty Publications

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso, and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence …

Seagrass–Pathogen Interactions: ‘Pseudo-Induction’ Of Turtlegrass Phenolics Near Wasting Disease Lesions, Latina Steele, Melanie Caldwell, Anne Boettcher, Tom Arnold

Biology Faculty Publications

Marine protists of the genus Labyrinthula cause the seagrass wasting disease, which is associated with regional die-offs of eelgrass Zostera marina and also infects turtlegrass Thalassia testudinum . The ability of seagrasses to resist pathogen attack is determined by multiple factors, which are poorly understood. One factor hypothesized to influence seagrass disease resistance is the presence of (poly)phenolic natural products such as caffeic acid, which inhibits the growth of L. zosterae in in vitro laboratory bioassays. This hypothesis has been supported by reports of pathogen-induced phenolic accumulations in eelgrass Z. marina. To test the response of T. testudinum to …

Complete Deletion Of Wheat Streak Mosaic Virus Hc-Pro: A Null Mutant Is Viable For Systemic Infection, Drake C. Stenger, Roy C. French, Frederick E. Gildow

Department of Plant Pathology: Faculty Publications

A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.

First Report Of Crown And Stem Rot Of Orchid (Orchis Palustris) Caused By Sclerotinia Minor, C. Eken, S. Ercişli, A. Eşitken, E. Demirci, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

Orchis palustris Jacq. is a wild orchid native to wetlands in eastern Anatolia. During June of 2003, near Erzurum, Turkey, a decline of this orchid was observed in several meadows that had been irrigated for forage production. Stems were chlorotic, wilted, and collapsed. There was a soft, watery rot at the crowns and lower stems. White mycelium and black sclerotia formed on necrotic stem and crown tissues. The fungus was isolated from sclerotia on potato dextrose agar (PDA) and identified as Sclerotinia minor Jagger on the basis of small sclerotia (0.5 to 2.5 mm long) scattered throughout the colonies (2). …

Reclassification Of Xanthomonas Campestris Pv. Citri (Ex Hasse 1915) Dye 1978 Forms A, B/C/D, And E As X. Smithii Subsp. Citri (Ex Hasse) Sp. Nov. Nom. Rev. Comb. Nov., X. Fuscans Subsp. Aurantifolii (Ex Gabriel 1989) Sp. Nov. Nom. Rev. Comb. Nov., And X. Alfalfae Subsp. Citrumelo (Ex Riker And Jones) Gabriel Et Al., 1989 Sp. Nov. Nom. Rev. Comb. Nov.; X. Campestris Pv Malvacearum (Ex Smith 1901) Dye 1978 As X. Smithii Subsp. Smithii Nov. Comb. Nov. Nom. Nov.; X. Campestris Pv. Alfalfae (Ex Riker And Jones, 1935) Dye 1978 As X. Alfalfae Subsp. Alfalfae (Ex Riker Et Al., 1935) Sp. Nov. Nom. Rev.; And ‘‘Var. Fuscans’’ Of X. Campestris Pv. Phaseoli (Ex Smith, 1987) Dye 1978 As X. Fuscans Subsp. Fuscans Sp. Nov., Norman W. Schaad, Elena Postnikova, George H. Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Bacterial canker of citrus is a serious disease of citrus worldwide. Five forms of the disease have been described, cankers “A”, “B”, “C”, “D”, and “E”. Although considerable genetic diversity has been described among the causal agents of the five forms of citrus canker and supports multiple taxons, the causal agents currently are classified as pathovars citri (“A”), aurantifolii (“B/C/D”) and citrumelo (“E”) of a single species, Xanthomonas campestris pv. citri (or X. axonopodis pv. citri).To determine the taxonomic relatedness among strains of X. campestris pv. citri, we conducted DNA–DNA relatedness assays, sequenced the 16S-23S intergenic spacer (ITS) …

Aspergillus Nidulans UvsbAtr And ScaaNbs1 Genes Show Genetic Interactions During Recovery From Replication Stress And Dna Damage, Marcia Regina Von Zeska Kress Fagundes, Camile P. Semighini, Iran Malazavi, Marcela Savoldi, Joel Fernandes De Lima, Maria Helena De Souza Goldman, Steven D. Harris, Gustavo Henrique Goldman

Department of Plant Pathology: Faculty Publications

The ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsB- ^ATR and the Mre11 complex (scaA^NBS1). We demonstrate that there is an epistatic relationship between uvsB^ATR, the homolog of the ATR/MEC1 gene, and scaA^NBSI, the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaA^NBS1 expression during recovery from replication stress depends on uvsB^ATR. In addition, we also show that the formation of UvsC foci during recovery …