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Articles 10741 - 10770 of 11636

Full-Text Articles in Life Sciences

Sequence And Annotation Of The 288-Kb Atcv-1 Virus That Infects An Endosymbiotic Chlorella Strain Of The Heliozoon Acanthocystis Turfacea, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, James Hartigan, Artur J.P. Pfitzner, Ella Hoffart, James L. Van Etten Jan 2007

Sequence And Annotation Of The 288-Kb Atcv-1 Virus That Infects An Endosymbiotic Chlorella Strain Of The Heliozoon Acanthocystis Turfacea, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, James Hartigan, Artur J.P. Pfitzner, Ella Hoffart, James L. Van Etten

James Van Etten Publications

Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3.83, that is a symbiont in the heliozoon A. turfacea. The 288,047-bp ATCV-1 genome is the first virus to be sequenced that infects Chlorella SAG 3.83. ATCV-1 contains 329 putative protein-encoding and 11 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands and intergenic space is minimal. Thirty-four percent of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. For example, these …


Chlorella Viruses Contain Genes Encoding A Complete Polyamine Biosynthetic Pathway, Sascha Baumann, Adrienne Sander, James Gurnon, Giane M. Yanai-Balser, James L. Van Etten, Markus Piotrowski Jan 2007

Chlorella Viruses Contain Genes Encoding A Complete Polyamine Biosynthetic Pathway, Sascha Baumann, Adrienne Sander, James Gurnon, Giane M. Yanai-Balser, James L. Van Etten, Markus Piotrowski

James Van Etten Publications

Two genes encoding the putative polyamine biosynthetic enzymes agmatine iminohydrolase (AIH) and N-carbamoylputrescine amidohydrolase (CPA) were cloned from the chloroviruses PBCV-1, NY-2A and MT325. They were expressed in Escherichia coli to form C-terminal (His)6-tagged proteins and the recombinant proteins were purified by Ni2+- binding affinity chromatography. The biochemical properties of the two enzymes are similar to AIH and CPA enzymes from Arabidopsis thaliana and Pseudomonas aeruginosa. Together with the previously known virus genes encoding ornithine/arginine decarboxlyase (ODC/ADC) and homospermidine synthase, the chloroviruses have genes that encode a complete set of functional enzymes that synthesize the rare polyamine homospermidine from arginine …


Characterization Of Bacteriophages Of Pseudomonas Syringae Pv. Tomato, Sara E. Prior, Amanda J. Andrews, Russell O. Nordeen Jan 2007

Characterization Of Bacteriophages Of Pseudomonas Syringae Pv. Tomato, Sara E. Prior, Amanda J. Andrews, Russell O. Nordeen

Journal of the Arkansas Academy of Science

Bacteriophages from supernatants of the plant pathogenic bacteria Pseudomonas syringae pv. tomato (P. tomato) were isolated, enriched, and purified by density block centrifugation in cesium chloride (CsCl) step gradients. The DNA from purified phage was isolated and digested with the restriction endonucleases EcoRl or HindIII. Three different DNA fingerprint patterns were determined indicating 3 unique phage isolates. Genome sizes of the phage ranged from 40 to 52 kilobases (kB). Buoyant densities of phage particles in CsCI varied from 1.36 to 1.51 glml. Electron microscopy revealed a single morphological type with an elongated polyhedral head and a long tail indicating the …


Genetic Diversity In Populations Of Xanthomonas Campestris Pv. Campestris In Cruciferous Weeds In Central Coastal California, A. Ignatov, A. Sechler, E. L. Schuenzel, I. Agarkova, B. Oliver, A. K. Vidaver, N. W. Schaad Jan 2007

Genetic Diversity In Populations Of Xanthomonas Campestris Pv. Campestris In Cruciferous Weeds In Central Coastal California, A. Ignatov, A. Sechler, E. L. Schuenzel, I. Agarkova, B. Oliver, A. K. Vidaver, N. W. Schaad

Department of Plant Pathology: Faculty Publications

Xanthomonas campestris pv. campestris (X. campestris) infects a large number of cruciferous plants, including weeds. California has one of the largest and most diverse populations of wild cruciferous plants in the world. Although considerable information is available on the genetic diversity of X. campestris in commercial crop plants, nothing is known about the diversity in strains infecting weeds. To assess the genetic diversity among strains of X. campestris in weeds in noncultivated and cultivated areas, strains of the pathogen were isolated from populations of cruciferous weeds growing in coastal valley crop-production sites and from remote nonproduction sites along the …


Expression Analysis Of Rice Defense-Related Genes In Turfgrass In Response To Magnaporthe Grisea, Young-Ki Jo, Guo-Liang Wang, Michael J. Boehm Jan 2007

Expression Analysis Of Rice Defense-Related Genes In Turfgrass In Response To Magnaporthe Grisea, Young-Ki Jo, Guo-Liang Wang, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Magnaporthe grisea (anamorph = Pyricularia grisea) causes blast on rice (Oryza sativa) and gray leaf spot on turfgrass. Gray leaf spot is a serious disease on St. Augustinegrass (Stenotaphrum secundatum), perennial ryegrass (Lolium perenne), and tall fescue (Festuca arundinacea). Virulence assays performed in this study revealed that M. grisea collected from rice could also cause disease on St. Augustinegrass and tall fescue. One rice isolate, Che86061, caused similar disease reactions on susceptible cultivars of rice and St. Augustinegrass and an incompatible interaction on resistant cultivars of both species. To explore whether …


Genetic Analysis Of A Novel Alaska Barley Yellow Dwarf Virus In The Family Luteoviridae, N.L. Robertson, Roy C. French Jan 2007

Genetic Analysis Of A Novel Alaska Barley Yellow Dwarf Virus In The Family Luteoviridae, N.L. Robertson, Roy C. French

Department of Plant Pathology: Faculty Publications

A new plant virus belonging to the family Luteoviridae and isolated from diseased oat (Avena sativa L.) plants was discovered in Alaska in 2003. Even though plants with red/orange leaves were indicative of barley yellow dwarf disease, they were not reactive to specific antibodies corresponding to barley yellow dwarf virus (BYDV)-MAV, -PAV, -SGV, and cereal yellow dwarf virus-RPV from enzyme-linked immunosorbent assays (ELISA). An alternative RT-PCR assay that incorporated Shu-F/Yan-R primers for detection of BYDV-MAV, -PAS, -PAV, and SGV was effective in producing ~830-nt fragments that contained genomic sequences to the 3’-terminus of the polymerase gene (ORF 2), the …


Structure And Biosynthesis Of Heat-Stable Antifungal Factor (Hsaf), A Broad-Spectrum Antimycotic With A Novel Mode Of Action, Fengan Yu, Kathia Zaleta-Rivera, Xiangcheng Zhu, Justin Huffman, Jeffrey C. Millet, Steven D. Harris, Gary Yuen, Xing-Cong Li, Liangcheng Du Jan 2007

Structure And Biosynthesis Of Heat-Stable Antifungal Factor (Hsaf), A Broad-Spectrum Antimycotic With A Novel Mode Of Action, Fengan Yu, Kathia Zaleta-Rivera, Xiangcheng Zhu, Justin Huffman, Jeffrey C. Millet, Steven D. Harris, Gary Yuen, Xing-Cong Li, Liangcheng Du

Department of Plant Pathology: Faculty Publications

A screen for antifungal compounds from Lysobacter enzymogenes strain C3, a bacterial biological control agent of fungal diseases, has previously led to the isolation of heat-stable antifungal factor (HSAF). HSAF exhibits inhibitory activities against a wide range of fungal species and shows a novel mode of antifungal action by disrupting the biosynthesis of a distinct group of sphingolipids. We have now determined the chemical structure of HSAF, which is identical to that of dihydromaltophilin, an antifungal metabolite with a unique macrocyclic lactam system containing a tetramic acid moiety and a 5,5,6-tricyclic skeleton. We have also identified the genetic locus responsible …


Nucleic Acids Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin Nov 2006

Nucleic Acids Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin

Department of Plant Pathology: Faculty Publications

The present invention relates to isolated nucleic acid molecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtoD2 protein of Pseudomonas syringae pv. syringae DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid mol ecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to colonization …


Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour Nov 2006

Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour

Department of Plant Pathology: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 is a model pathogen of tomato and Arabidopsis that uses a hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS) to deliver virulence effector proteins into host cells. Expression of the Hrp system and many effector genes is activated by the HrpL alternative sigma factor. Here, an open reading frame-specific whole-genome microarray was constructed for DC3000 and used to comprehensively identify genes that are differentially expressed in wild-type and ΔhrpL strains. Among the genes whose differential regulation was statistically significant, 119 were upregulated and 76 were downregulated in the wild-type compared with the ΔhrpL …


Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde Oct 2006

Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde

Department of Plant Pathology: Faculty Publications

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewater samples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analyzed for microbial content as compared to soil without wastewater contamination in the neighborhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand, etc., were examined. The wastewater samples from both abattoirs were highly contaminated; Agege abattoir …


Genetic Characterization And Diversity Of Rathayibacter Toxicus, Irina V. Agarkova, A. K. Vidaver, E. N. Postnikova, I. T. Riley, N. W. Schaad Oct 2006

Genetic Characterization And Diversity Of Rathayibacter Toxicus, Irina V. Agarkova, A. K. Vidaver, E. N. Postnikova, I. T. Riley, N. W. Schaad

Department of Plant Pathology: Faculty Publications

Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and human toxin that is often fatal to livestock and has a history of occurring in unexpected circumstances. DNA of 22 strains of R. toxicus from Australia were characterized using amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE). AFLP analysis grouped the 22 strains into three genetic clusters that correspond to their geographic origin. The mean similarity between the three clusters was 85 to 86%. PFGE analysis generated three different banding patterns that enabled typing the strains …


Emended Classification Of Xanthomonad Pathogens On Citrus, Norman W. Schaad, Elena Postnikova, George Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver Oct 2006

Emended Classification Of Xanthomonad Pathogens On Citrus, Norman W. Schaad, Elena Postnikova, George Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

In the paper by Schaad et al. [24] on reclassification of several xanthomonads, nomenclatural errors were made. The name Xanthomonas smithii subsp. citri proposed for the former taxon X. campestris pv. citri ( = X. axonopodis pv. citri) is illegitimate. Following the reinstatement of X. citri (ex Hasse 1915) Gabriel et al. [9] as a validly published name, Young et al. [34] wrote that the reinstatement of this epithet was based on a description that was inadequate in terms of modern practice for the purpose of formal classification. This report was subsequently summarized by the International Committee on …


Transcriptome Analysis Of Aspergillus Nidulans Exposed To Camptothecin-Induced Dna Damage, Iran Malazavi, Marcela Savoldi, Sonia Marla Zingaretti Di Mauro, Carlos Frederico Martins Menck, Steven D. Harris, Maria Helena De Souza Goldman, Gustavo Henrique Goldman Oct 2006

Transcriptome Analysis Of Aspergillus Nidulans Exposed To Camptothecin-Induced Dna Damage, Iran Malazavi, Marcela Savoldi, Sonia Marla Zingaretti Di Mauro, Carlos Frederico Martins Menck, Steven D. Harris, Maria Helena De Souza Goldman, Gustavo Henrique Goldman

Department of Plant Pathology: Faculty Publications

We have used an Aspergillus nidulans macroarray carrying sequences of 2,787 genes from this fungus to monitor gene expression of both wild-type and uvsBATR (the homologue of the ATR gene) deletion mutant strains in a time course exposure to camptothecin (CPT). The results revealed a total of 1,512 and 1,700 genes in the wild-type and uvsBATR deletion mutant strains that displayed a statistically significant difference at at least one experimental time point. We characterized six genes that have increased mRNA expression in the presence of CPT in the wild-type strain relative to the uvsBATR mutant strain: fhdA …


Dna Molecules And Polypeptides Of Pseudomonas Syringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 7,102,059 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski Sep 2006

Dna Molecules And Polypeptides Of Pseudomonas Syringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 7,102,059 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski

Department of Plant Pathology: Faculty Publications

One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can …


Cloning Of Nt.Cviqii Nicking Endonuclease And Its Cognate Methyltransferase: M.Cviqii Methylates Ag Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, Shuang-Yong Xu Sep 2006

Cloning Of Nt.Cviqii Nicking Endonuclease And Its Cognate Methyltransferase: M.Cviqii Methylates Ag Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, Shuang-Yong Xu

Department of Plant Pathology: Faculty Publications

Chlorella virus NY-2A has a large, highly methylated dsDNA genome (45% of the cytosines are 5-methylcytosine and 37% of the adenines are N6-methyladenine). Here, we report the cloning, expression, and characterization of the NY-2A-encoded CviQII nicking-modification (N-M) system. The nicking endonuclease, Nt.CviQII, recognizes R ↓ AG (R = A or G, ↓ indicating cleavage site) sequences and cleaves the phosphodiester bond 5′ to the adenosine. Because of the difficulty in cloning and expressing the wild-type Nt.CviQII, C-terminal truncation mutants were generated and full-length Nt.CviQII was reconstructed by intein-mediated peptide ligation. The truncation mutants and the reconstructed full-length Nt.CviQII …


Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano Sep 2006

Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and translocated into plant cells by the P. syringae pv. tomato DC3000 TTSS. A DC3000 hrpJ mutant, UNL140, was greatly reduced in its ability to cause disease symptoms and multiply in Arabidopsis thaliana …


Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist Sep 2006

Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2apol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B33' lacked the coat protein gene and negative-strand RNA promoter. B35' lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B33' and B35' transcription was repressed and Ura+ yeast cells were selected. All Ura+ cells …


Sheep Updates 2006 - Part 2, Darryl Smith, Kathryn Kemper, David Rutley, R. R. Woolaston, D. J. Brown, K. D. Atkins, A. E. Casey, A. J. Ball, David Hopkins, David Stanley, Leonie Martin, Arthur Gilmour, Remy Van De Ven, Geoff Duddy, Steve Semple, David Kessell, Andrew Thompson Jul 2006

Sheep Updates 2006 - Part 2, Darryl Smith, Kathryn Kemper, David Rutley, R. R. Woolaston, D. J. Brown, K. D. Atkins, A. E. Casey, A. J. Ball, David Hopkins, David Stanley, Leonie Martin, Arthur Gilmour, Remy Van De Ven, Geoff Duddy, Steve Semple, David Kessell, Andrew Thompson

Sheep Updates

This session covers six papers from different authors:

GENETICS

1. Novel selection traits - what are the possible side effects?, Darryl Smith, Kathryn Kemper, South Australian Research and Development Institute, David Rutley, University of Adelaide.

2. Genetic Changes in the Australian Merino since 1900, Sheep Genetics Australia Technical Committee, R.R. Woolaston Pullenvale, Queensland, D.J. Brown, Animal Genetics and Breeding Unit*, University of New England, K.D. Atkins, A.E. Casey, NSW Department of Primary Industries, A.J. Ball, Meat and Livestock Australia, University of New England

3. Influence of Sire Growth Estimated Breeding Value (EBV0 on Progeny Growth, David Hopkins, David Stanley, Leonie …


Nested Deletion Analysis Of Wheat Streak Mosaic Virus Hc-Pro: Mapping Of Domains Affecting Polyprotein Processing And Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Roy C. French Jun 2006

Nested Deletion Analysis Of Wheat Streak Mosaic Virus Hc-Pro: Mapping Of Domains Affecting Polyprotein Processing And Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Roy C. French

Department of Plant Pathology: Faculty Publications

A series of in-frame and nested deletion mutations which progressively removed 5′-proximal sequences of the Wheat streak mosaic virus (WSMV) HC-Pro coding region (1152 nucleotides) was constructed and evaluated for pathogenicity to wheat. WSMV HC-Pro mutants with 5′- proximal deletions of 12 to 720 nucleotides systemically infected wheat. Boundary sequences flanking the deletions were stable and unaltered by passage through plants for all deletion mutants except HCD12 (lacking HC-Pro codons 3–6) that exhibited strong bias for G to A substitution at nucleotide 1190 in HC-Pro codon 2 (aspartic acid to asparagine). HC-Pro mutants with 5′-proximal deletions of up to 720 …


Functional Characterization Of Aspergillus Nidulans Homologues Of Saccharomyces Cerevisiae Spa2 And Bud6, Aleksandra Virag, Steven D. Harris Jun 2006

Functional Characterization Of Aspergillus Nidulans Homologues Of Saccharomyces Cerevisiae Spa2 And Bud6, Aleksandra Virag, Steven D. Harris

Department of Plant Pathology: Faculty Publications

The importance of polarized growth for fungi has elicited significant effort directed at better understanding underlying mechanisms of polarization, with a focus on yeast systems. At sites of tip growth, multiple protein complexes assemble and coordinate to ensure that incoming building material reaches the appropriate destination sites, and polarized growth is maintained. One of these complexes is the polarisome that consists of Spa2, Bud6, Pea2, and Bni1 in Saccharomyces cerevisiae. Filamentous hyphae differ in their development and life style from yeasts and likely regulate polarized growth in a different way. This is expected to reflect on the composition and …


An Assessment Model For Rating High-Threat Crop Pathogens, N. W. Schaad, J. Abrams, L. V. Madden, R. D. Frederick, D. G. Luster, V. D. Damsteegt, A. K. Vidaver May 2006

An Assessment Model For Rating High-Threat Crop Pathogens, N. W. Schaad, J. Abrams, L. V. Madden, R. D. Frederick, D. G. Luster, V. D. Damsteegt, A. K. Vidaver

Department of Plant Pathology: Faculty Publications

Natural, accidental, and deliberate introductions of nonindigenous crop pathogens have become increasingly recognized as threats to the U.S. economy. Given the large number of pathogens that could be introduced, development of rapid detection methods and control strategies for every potential agent would be extremely difficult and costly. Thus, to ensure the most effective direction of resources a list of high-threat pathogens is needed. We address development of a pathogen threat assessment model based on the analytic hierarchy process (AHP) that can be applied worldwide, using the United States as an illustrative example. Previously, the AHP has been shown to work …


New Outbreaks Of Bacterial Wilt Of Dry Bean In Nebraska Observed From Field Infections, R. M. Harveson, H. F. Schwartz, A. K. Vidaver, Patricia A. Lambrecht, K. L. Otto May 2006

New Outbreaks Of Bacterial Wilt Of Dry Bean In Nebraska Observed From Field Infections, R. M. Harveson, H. F. Schwartz, A. K. Vidaver, Patricia A. Lambrecht, K. L. Otto

Department of Plant Pathology: Faculty Publications

Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens was one of the more problematic diseases of dry bean (Phaseolus vulgaris L.) throughout the irrigated High Plains (Colorado, Nebraska, and Wyoming) in the 1960s and early 1970s, but has not been observed since that time. However, in August of 2003, plants exhibiting wilting and irregular, interveinal necrotic foliar lesions surrounded by a bright yellow border were found in three dry bean fields (market class Great Northern) in Scotts Bluff County, Nebraska. During 2004, plants exhibiting identical symptoms were additionally found occurring in more than 40 dry bean fields in western …


Association Of Plant Color And Pericarp Color With Colonization Of Grain By Members Of Fusarium And Alternaria In Near-Isogenic Sorghum Lines, Deanna L. Funnell, Jeffrey F. Pedersen Apr 2006

Association Of Plant Color And Pericarp Color With Colonization Of Grain By Members Of Fusarium And Alternaria In Near-Isogenic Sorghum Lines, Deanna L. Funnell, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

White sorghum (Sorghum bicolor) grain from tan plants is more desirable for human or animal consumption. Colonization by Fusarium and Alternaria spp. was assessed for near-isogenic lines differing in wound response (purple or tan) and pericarp color (red or white) in field-grown grain and in greenhouse-grown plants. Seeds were screened on a semi-selective medium for Alternaria and Fusarium. Significantly fewer fungal colonies were obtained from tan plants with white seed, and fewer numbers of Alternaria colonies were obtained from white seed, regardless of plant color, from an irrigated field, while there were no differences in fungal composition …


Phycodnaviruses: A Peek At Genetic Diversity, David Dunigan, Lisa A. Fitzgerald, James L. Van Etten Apr 2006

Phycodnaviruses: A Peek At Genetic Diversity, David Dunigan, Lisa A. Fitzgerald, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The family Phycodnaviridae encompasses a diverse collection of large icosahedral, dsDNA viruses infecting algae. These viruses have genomes ranging from 160 to 560 kb. The family consists of six genera based initially on host range and supported by sequence comparisons. The family is monophyletic with branches for each genus, but the phycodnaviruses have evolutionary roots that connect with several other families of large DNA viruses, referred to as the nucleocytoplasmic large DNA viruses (NCLDV). The genomes of members in three genera in the Phycodnaviridae have recently been sequenced and the purpose of this manuscript is to summarize these data. The …


Proceedings Of The 33rd Annual Meeting, Southern Soybean Disease Workers (March 8-9, 2006, Jackson, Tennessee), Michael E. Schmidt, Ray Schneider, Daniel H. Poston, Jason Bond, Steve R. Koenning Mar 2006

Proceedings Of The 33rd Annual Meeting, Southern Soybean Disease Workers (March 8-9, 2006, Jackson, Tennessee), Michael E. Schmidt, Ray Schneider, Daniel H. Poston, Jason Bond, Steve R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern Soybean Disease Workers 2005 treasury report

Southern United States Soybean Disease Loss Estimates for 2005. Compiled by SR Koenning

Virulence in Phytopbthora sojae Isolates to Soybeans with Rps8 Resistance. DA Smith, TS Abney, and JG Shannon

New Sources of Resistance to SCN in Soybean. PR Arelli

Soybean Disease Management in Louisiana. B Padgett, MA Purvis, and BW Garber

Efficacy and Profitability of Foliar Fungicide in the Absence of Soybean Rust. M Newman and W Percell

Fungicide and Insecticide Combinations for Enhancing Soybean Health and Yield. RP Mulrooney and RW Taylor

Overview of Soybean Rust Monitoring in the US. …


Reaction Of Sorghum Lines Genetically Modified For Reduced Lignin Content To Infection By Fusarium And Alternaria Spp., Deanna L. Funnell, Jeffrey F. Pedersen Mar 2006

Reaction Of Sorghum Lines Genetically Modified For Reduced Lignin Content To Infection By Fusarium And Alternaria Spp., Deanna L. Funnell, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Two genes conferring the brown midrib (bmr) trait had been backcrossed into six elite sorghum lines, resulting in reduced lignin in the bmr lines when compared with the wild-type parent. Seed and leaf tissue from field-grown plants, planted at two locations, were screened for Alternariu spp. and Fusarium spp. on semi-selective media. The results suggest that bmr lines do not have increased susceptibility to colonization by Alternariu spp. However, significantly fewer colonies of Fusarium spp., including Fusarium moniliforme, were recovered from seed of reduced lignin lines from two genetic backgrounds. That the bmr trait in some genetic backgrounds might …


Potassium Ion Channels Of Chlorella Viruses Cause Rapid Depolarization Of Host Cells During Infection, Florian Frohns, Anja Kasmann, Detlef Kramer, Britta Schafer, Mario Mehmel, Ming Kang, James L. Van Etten, Sabrina Gazzarrini, Ana Morino, Gerhard Thiel Mar 2006

Potassium Ion Channels Of Chlorella Viruses Cause Rapid Depolarization Of Host Cells During Infection, Florian Frohns, Anja Kasmann, Detlef Kramer, Britta Schafer, Mario Mehmel, Ming Kang, James L. Van Etten, Sabrina Gazzarrini, Ana Morino, Gerhard Thiel

Department of Plant Pathology: Faculty Publications

revious studies have established that chlorella viruses encode K+ channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these channels to Cs+ to determine if the membrane depolarization observed during virus infection is caused by the activities of these channels. Infection of Chlorella NC64A with four viruses caused rapid membrane depolarization of similar amplitudes, but with different kinetics. Depolarization was fastest after infection with virus SC-1A (half time [t1/2], about 9 min) and slowest with virus NY-2A (t1/2, about 12 min). Cs+ inhibited …


Polarity In Plants - Annual Plant Reviews, Volume 12, Steven D. Harris Mar 2006

Polarity In Plants - Annual Plant Reviews, Volume 12, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Polarity is a fundamental property of all cells. The molecular mechanisms that underlies the establishment and maintenance of cellular polarity have been intensively studied in animal cells and yeast-like fungi. These studies reveal an emerging general principle whereby positional information is relayed to the cytoskeleton and other morphogenetic functions by conserved signaling pathways. Comparatively less attention has been directed toward the molecular basis of polarized growth in plant cells and tissues. This volume, Polarity in Plants, provides a comprehensive review of the topic that should satisfy both hardcore aficionados of plant cell and developmental biology as well as curious investigators …


Bacillus Species Nrrl B-30212 For Reducing Fusarium Head Blight In Cereals, David A. Schisler, Naseem L. Khan, Michael J. Boehm Feb 2006

Bacillus Species Nrrl B-30212 For Reducing Fusarium Head Blight In Cereals, David A. Schisler, Naseem L. Khan, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Four yeasts (NRRL Y-30213, NRRL Y-30214, NRRL Y-30215, and NRRL Y-30216) and 1 bacterium (NRRL B-30212) have been identified as being superior antagonists capable of suppressing Fusarium head blight (head scab) in cereals, particularly in wheat and barley. Fusarium head blight is primarily caused by the fungus Gibberella zeae (anamorph=Fusarium graminearum).


Crop Updates 2006 - Weeds, Alexandra Douglas, Thomas M. Wolf, Harm Van Rees, Bill Gordon, Peter Newman, Glenn Adam, Aik Lee, Siew Lee, Katherine Hollaway, Brad Rayner, John Peirce, Rick Llewellyn, Frank D'Emden, Michelle Owen, Stephen Powles, Michael Walsh, Emma Glasfurd, Kathryn Steadman, David Ferris, Bevan Addison, Aik Cheam, Dave Nicholson, Ruben Vargas, Shahab Pathan, Abul Hashem, Nerys Wilkins, Catherine Borger, Bob French, Kari-Lee Falconer, Martin Harries, Chris Matthews, Vikki Osten, Harnohinder Dhammu, David Nicholson, Richard Quinlan, Debbie Allen, Mark Seymour, Pam Burgess, Owen Coppen, Chris Roberts, Christiaan Valentine, A. K. Basandrai, W. J. Macleod, John Moore, Neil Rothnie, Russell Speed, John Andrew Simons, Ted Spadek, Daya Patabendige, Michael Renton, Sally Peltzer, Art Diggle Feb 2006

Crop Updates 2006 - Weeds, Alexandra Douglas, Thomas M. Wolf, Harm Van Rees, Bill Gordon, Peter Newman, Glenn Adam, Aik Lee, Siew Lee, Katherine Hollaway, Brad Rayner, John Peirce, Rick Llewellyn, Frank D'Emden, Michelle Owen, Stephen Powles, Michael Walsh, Emma Glasfurd, Kathryn Steadman, David Ferris, Bevan Addison, Aik Cheam, Dave Nicholson, Ruben Vargas, Shahab Pathan, Abul Hashem, Nerys Wilkins, Catherine Borger, Bob French, Kari-Lee Falconer, Martin Harries, Chris Matthews, Vikki Osten, Harnohinder Dhammu, David Nicholson, Richard Quinlan, Debbie Allen, Mark Seymour, Pam Burgess, Owen Coppen, Chris Roberts, Christiaan Valentine, A. K. Basandrai, W. J. Macleod, John Moore, Neil Rothnie, Russell Speed, John Andrew Simons, Ted Spadek, Daya Patabendige, Michael Renton, Sally Peltzer, Art Diggle

Crop Updates

This session covers thirty seven papers from different authors:

1. ACKNOWLEDGEMENTS, Alexandra Douglas, CONVENOR – WEEDS DEPARTMENT OF AGRICULTURE

SPRAY TECHNOLOGY

2. Meeting the variable application goals with new application technology, Thomas M. Wolf, Agriculture and Agri-Food Canada, Saskatoon Research Centre

3. Spray nozzles for grass weed control, Harm van Rees, BCG (Birchip Cropping Group)

4. Boom sprayer setups – achieving coarse droplets with different operating parameters, Bill Gordon, Bill Gordon Consulting

5. Complying with product label requirements, Bill Gordon, Bill Gordon Consulting

6. IWM a proven performer over 5 years in 33 focus paddocks, Peter Newman and …