Life Sciences Commons^™

Plant Virus Hc-Pro Is A Determinant Of Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Frederick E. Gildow, Kempton M. Horken, Roy C. French

Department of Plant Pathology: Faculty Publications

The eriophyid mite transmitted Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) shares a common genome organization with aphid transmitted species of the genus Potyvirus. Although both tritimoviruses and potyviruses encode helper component-proteinase (HC-Pro) homologues (required for nonpersistent aphid transmission of potyviruses), sequence conservation is low (amino acid identity, ~16%), and a role for HC-Pro in semipersistent transmission of WSMV by the wheat curl mite (Aceria tosichella [Keifer]) has not been investigated. Wheat curl mite transmissibility was abolished by replacement of WSMV HC-Pro with homologues of an aphid transmitted potyvirus (Turnip mosaic virus), …

Pseudomonas Syringae Type Iii Chaperones Shco1, Shcs1, And Shcs2 Facilitate Translocation Of Their Cognate Effectors And Can Substitute For Each Other In The Secretion Of Hopo1-1, Ming Guo, Scott T. Chancey, Fang Tian, Zhengxiang Ge, Yashitola Jamir, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The Pseudomonas syringae type III secretion system (TTSS) translocates effector proteins into plant cells. Several P. syringae effectors require accessory proteins called type III chaperones (TTCs) to be secreted via the TTSS. We characterized the hopO1-1, hopS1, and hopS2 operons in P. syringae pv. tomato DC3000; these operons encode three homologous TTCs, ShcO1, ShcS1, and ShcS2. ShcO1, ShcS1, and ShcS2 facilitated the type III secretion and/or translocation of their cognate effectors HopO1-1, HopS1, and HopS2, respectively. ShcO1 and HopO1-1 interacted with each other in yeast two-hybrid and coimmunoprecipitation assays. Interestingly, ShcS1 and ShcS2 were capable of substituting for …

Mutagenesis Of Β-1,3-Glucanase Genes In Lysobacter Enzymogenes Strain C3 Results In Reduced Biological Control Activity Toward Bipolaris Leaf Spot Of Tall Fescue And Pythium Damping-Off Of Sugar Beet, Jeffrey D. Palumbo, Gary Y. Yuen, C. Christine Jochum, Kristin Tatum, Donald Y. Kobayashi

Department of Plant Pathology: Faculty Publications

Lysobacter enzymogenes produces extracellular lytic enzymes capable of degrading the cell walls of fungi and oomycetes. Many of these enzymes, including β-1,3-glucanases, are thought to contribute to the biological control activity expressed by several strains of the species. L. enzymogenes strain C3 produces multiple extracellular β-1,3-glucanases encoded by the gluA, gluB, and gluC genes. Analysis of the genes indicates they are homologous to previously characterized genes in the related strain N4-7, each sharing >95% amino acid sequence identity to their respective counterparts. The gluA and gluC gene products encode enzymes belonging to family 16 glycosyl …

Green Fluorescent Protein Is Lighting Up Fungal Biology, J.M. Lorang, R.P. Tuori, J.P. Martinez, T.L. Sawyer, R.S. Redman, J.A. Rollins, T. J. Wolpert, K.B. Johnson, R. J. Rodriguez, M. B. Dickman, L. M. Ciuffetti

Department of Plant Pathology: Faculty Publications

Prasher (42) cloned a cDNA for the green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria in 1992. Shortly thereafter, to the amazement of many investigators, this gene or derivatives thereof were successfully expressed and conferred fluorescence to bacteria and Caenorhabditis elegans cells in culture (10, 31), followed by yeast (24, 39), mammals (40), Drosophila (66), Dictyostelium (23, 30), plants (28, 49), and filamentous fungi (54). The tremendous success of GFP as a reporter can be attributed to unique qualities of this 238- amino-acid, 27-kDa protein which absorbs light at maxima of 395 and 475 nm and emits light …

'Paclims': A Component Lim System For High-Throughput Functional Genomic Analysis, Nicole Donofrio, Ravi Rajagopalon, Douglas Brown, Stephen Diener, Donald Windham, Shelly Nolin, Anna Floyd, Thomas Mitchell, Natalia Galadima, Sara Tucker, Marc J. Orbach, Gayatri Patel, Mark Farman, Vishal Pampanwar, Cari Soderlund, Yong-Hwan Lee, Ralph A. Dean

Plant Pathology Faculty Publications

BACKGROUND: Recent advances in sequencing techniques leading to cost reduction have resulted in the generation of a growing number of sequenced eukaryotic genomes. Computational tools greatly assist in defining open reading frames and assigning tentative annotations. However, gene functions cannot be asserted without biological support through, among other things, mutational analysis. In taking a genome-wide approach to functionally annotate an entire organism, in this application the approximately 11,000 predicted genes in the rice blast fungus (Magnaporthe grisea), an effective platform for tracking and storing both the biological materials created and the data produced across several participating institutions was required.

RESULTS: …

Proposed Guidelines For A Unified Nomenclature And Phylogenetic Analysis Of Type Iii Hop Effector Proteins In The Plant Pathogen Pseudomonas Syringae, Magdalen Lindeberg, John Stavrinides, Jeffrey H. Chang, James R. Alfano, Alan Collmer, Jeffrey L. Dangl, Jean T. Greenberg, John W. Mansfield, David S. Guttman

Department of Plant Pathology: Faculty Publications

Pathovars of Pseudomonas syringae interact with their plant hosts via the action of Hrp outer protein (Hop) effector proteins, injected into plant cells by the type III secretion system (TTSS). Recent availability of complete genome sequences for a number of P. syringae pathovars has led to a significant increase in the rate of effector discovery. However, lack of a systematic nomenclature has resulted in multiple names being assigned to the same Hop, unrelated Hops designated by the same alphabetic character, and failure of name choices to reflect consistent standards of experimental confirmation or phylogenetic relatedness. Therefore, specific experimental and bioinformatic …

Impact Of Reduced Lignin On Plant Fitness, Jeffrey F. Pedersen, Kenneth P. Vogel, Deanna L. Funnell

Department of Plant Pathology: Faculty Publications

Lignin content of crop plants has been reduced by traditional plant breeding, natural and induced mutations, and insertion of transgenes. The effects of these genes and associated lower lignin content have been examined in terms of agricultural fitness or with regard to economically harvestable yields of useful plant products, or, in the case of some perennial species, survivability over multiple years. In general, crop yields are depressed by significant reductions in lignin content. Other negative effects observed in plants with lowered lignin contents include lodging and reduction of long-term survival of some perennial species. However, the interactions of genes involved …

Crop Updates 2005 - Geraldton, David Stephens, Nicola Telcik, Cameron Weeks, Richard Quinlan, Tony Harman, Wal Anderson, Vivien A. Vanstone, Sean J. Kelly, Helen F. Turner, Mena C. Gilchrist, Ciara Beard, Geoff Thomas, Robert Loughman, Kith Jayasena, Manisha Shenkar, Michael Walsh, Mechelle Owen, Stephen Powels, Rob Grima, Andrew Blake, Bill Bowden, Mike Collins, Luigi Moreschi, Douglas Hamilton, Russell Speed, Neil Rothnie, John Andrew Simons, Ted Spadek, John Moore, Graham Walton, Nadine Eva, Bindi Webb, Damian Shepherd, David Forrester, Peter Tozer, Roy Butler, Keith Croker, Nathan Windebank

Crop Updates

This session covers seventeen papers from different authors

1. 2005 Seasonal Outlook, David Stephens and Nicola Telcik, Department of Agriculture

2. Horses for Courses – using the best tools to manage climate risk, Cameron Weeks, Mingenew-Irwin Group / Planfarm and Richard Quinlan, Planfarm Agronomy

3. Global influences driving Australian agriculture, Tony Harman, Australian Government Department of Agriculture, Fisheries and Forestry

4. Wheat yield and quality improvements – where have they come from and can we have more? Wal Anderson, Department of Agriculture

5. Rotations for nematode management, Vivien A. Vanstone, Sean J. Kelly, Helen F. Hunter and Mena C. Gilchrist …

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Proceedings Of The 32nd Annual Meeting, Southern Soybean Disease Workers (March 2-3, 2005, Scottsdale, Arizona), Ray Schneider, Boyd Padgett, Michael Schmidt, Jason Bond, Stephen R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern Soybean Disease Workers 2004 treasury report

Southern United States Soybean Disease Loss Estimates for 2004. Compiled by SR Koenning

Initial Discovery of Asian Soybean Rust in Louisiana in 2004 and Lessons for 2005. RW Schneider, CA Hollier, and HK Wbitam

Efficacy of myclobutanil for control of Asian soybean rust in Brazil (2004). MT Iarnauti, NA Alvarenga, JA Rosseto, GR Tofoli, BD Olson, DG Ouimette, and DC Ruen.

Flutriafol - a triazole fungicide giving strong curative action and persistence of effect against Asian soybean rust. T Baker and R Noon Cherninova

Experience in Use of Syngenta Fungicides for Controlling …

Registration Of Common Bacterial Blight Resistant Pinto Bean Germplasm Line Abcp-8, N. Mutlu, P.N. Miklas, J. R. Steadman, A. K. Vidaver, Dale T. Lindgren, J. Reiser, D.P. Coyne, M. A. Pator-Corrales

Department of Plant Pathology: Faculty Publications

Pinto bean (Phaseolus vulgaris L.) germplasm line ABCP-8 (Reg. no. GP-237, PI 635118) was developed by the University of Nebraska Agricultural Research Division in cooperation with USDA-ARS and released in 2004. This line was bred specifically for enhanced resistnace to common bacterial blight [caused by Xanthomonas campestris pv. phaseoli (Smith) Dye]. Pinto and other dry bean market classes (dark red kidney, great northern, navy, etc.) grown in the U.S.A. east of the continental divide are often attacked by common bacterial blight, a seed-transmitted disease that causes up to 40% yield loss in susceptible cultivars as well as reduction of …

Crop Updates 2005 - Cereals, Darshan Sharma, Brenda Shackley, Mohammad Amjad, Christine M. Zaicou-Kunesch, Wal Anderson, D. Tennant, Steve Penny Jr, Ben Curtis, Veronika Reck, Richard Richards, Gavin Knell, Steve Curtin, David Sermon, Alan Bedggood, T. B. Biddulph, T. L. Setter, J. A. Plummer, D. J. Mares, H. Khabaz-Saberi, I. Waters, G. Mcdonald, Harmohinder Dhammu, David Nicholson, Chris Roberts, Stephen Loss, Ross Brennan, Kithsiri Jayasena, Eddy Pol, Simon Teakle, Rob Loughman, Ciara Beard, Blakely Paynter, K. Tanaka, G. Poulish, A. Smith, Paul Damon, Zed Rengel, Lionel Martin, Narelle Hill, Ron Mctaggart, Ray Tugwell, Geoff J. Thomas, Manisha Shankar, John Majewski, D. Foster, H. Golzar, J. Piotrowski, Rob Grima, Jeff Russell, Angie Roe, Vivien A. Vanstone, Sean Kelly, Helen Hunter, Mena C. Gilchrist, C. R. Newman, Svetlana Micic, Phil Michael, Gabrielle Coupland, Ern Kostas, Mohammed Hamza, Tina Botwright Acuña, Len Wade, Ingrid Richardson

Crop Updates

This session covers thirty six papers from different authors:

WHEAT AGRONOMY

1. Optimum sowing time of new wheat varieties in Western Australia, Darshan Sharma, Brenda Shackley, Mohammad Amjad, Christine M. Zaicou-Kunesch and Wal Anderson, Department of Agriculture

2. Wheat varieties updated in ‘Flowering Calculator’: A model predicting flowering time, B. Shackley, D. Tennant, D. Sharma and C.M. Zaicou-Kunesch, Department of Agriculture

3. Plant populations for wheat varieties, Christine M. Zaicou-Kunesch, Wal Anderson, Darshan Sharma, Brenda Shackley and Mohammad Amjad, Department of Agriculture

4. New wheat cultivars response to fertiliser nitrogen in four major agricultural regions of Western Australia, Mohammad Amjad, …

Crop Updates 2005 - Lupins And Pulses, Peter White, Martin Harries, Ian Pritchard, Rodger Beermier, Mark Seymor, David Syme, Bevan Buirchell, Kedar Adhikari, Mark Sweetingham, Geoff Thomas, Jon Clements, Shao Fang Wang, Ping Si, David Bowran, Huaan Yang, Mike Baker, Wayne Parker, Bob French, Damien Owen D'Arcy, Bill Bowden, Craig Scanlan, Jeff Russell, Angie Roe, Greg Shea, Chris Matthews, Shahab Pathan, Abul Hashem, Sofia Sipsas, John Quealy, Roger Jones, Clive Francis, Colin Smith, Gordon Francis, Tanveer Khan, Kerry Regan, Jenny Garlinge, Rod Hunter, Greg Mussell, Glen Riethmuller, Moin Salam, Jean Galloway, Larn Mcmurray, Kambot Siddique, Dirranie Kirby, Murray Blyth, Fucheng Shan, Heather Clarke, Madeleine Wouterlood, Hans Lambers, Erik Veneklaas, Peter Smith, Gae Plunkett, Tim Pope, Janine Croser, Julia Wilson, Leanne Young, Michael Materne, Colin Hanbury, Dennis Van Gool, Noel Schoknecht, Richard Bell, Wendy Vance, Kedar Adhikari, Mohammad Nuruzzaman, Erik Veneklaas, Mike Bolland, Stuart Pearse, Geoff Thomas, Ken Adcock, Bill Mcleod, Pip Payne, Alan Harrod, Glenda Smith, Rohan Prince

Crop Updates

This session covers sixty five papers from different authors:

1. 2004 LUPIN AND PULSE INDUSTRY HIGHLIGHTS, Peter White Department of Agriculture

2. BACKGROUND, Peter White Department of Agriculture

2004 REGIONAL ROUNDUP

3. Northern Agricultural Region, Martin Harries, Department of Agriculture

4. Central Agricultural Region, Ian Pritchard, Department of Agriculture

5. Great Southern and Lakes, Rodger Beermier, Department of Agriculture

6. Esperance Port Zone, Mark Seymour, Department of Agriculture, and David Syme, The Grain Pool of WA

LUPIN AND PULSE PRODUCTION AGRONOMY AND GENETIC IMPROVEMENT

7. Lupin, Martin Harries, Department of Agriculture …

Crop Updates 2005 Oilseeds, John Duff, Douglas Hamilton, Graham Walton, Hasan Zaheer, Neil Harris, Heather Cosgriff, Margaret C. Campbell, Bill Crabtree, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Russel Speed, Neil Rothnie, John Andrew Simons, Ted Spadeck, John Moore, Brenda Coutts, Roger Jones, Neil Harris, Moin Salam, Ravjit Khangura, Art Diggle

Crop Updates

This session covers fifteen papers from different authors:

1. ACKNOWLEDGEMENTS, Douglas Hamilton, FARMING SYSTEMS DEVELOPMENT OFFICER

CROP AGRONOMY AND NUTRITION

2. Canola workshop at Crop Updates 2005, Oilseeds WA, John Duff, EXECUTIVE OFFICER OILSEEDS WA

3. Comparison of IT and TT canola varieties in geographic zones of WA, 2003-4, Graham Walton and Hasan Zaheer, Department of Agriculture

4. Farmer scale canola variety trials in WA, 2004, Graham Walton, John Duff, Neil Harris and Heather Cosgriff, Oilseeds WA

5. Oilseed crops for industrial uses, Margaret C. Campbell, Centre for Legumes in Mediterranean Agriculture (CLIMA), Graham Walton,Department of Agriculture

6. …

Crop Updates 2005 - Farming Systems, David Stephens, Nicola Telcik, Ross Kingwell, Wayne Pluske, Bill Bowden, Mike Collins, Frances Hoyle, D. V. Murphy, N. Milton, M. Osman, L. K. Abbott, W. R. Cookson, S. Darmawanto, Bill Crabtree, Geoff Anderson, Darren Kidson, Ross Brennan, Nick Drew, Craig Scanlan, Lisa Sherriff, Bob French, Reg Lunt, Jeff Russell, Angie Roe, Ian Maling, Matthew Adams, George Yan, Mohammad Hamza, Glen Riethmuller, Wal Anderson, Angela Loi, Phil Nichols, Clinton Revell, David Ferris, Phil Ward, Andrea Hills, Sally-Anne Penny, David Hall, Michael Robertson, Don Gaydon, Tress Walmsley, Caroline Peek, Megan Abrahams, Paul Raper, Richard O'Donnell, Trevor Lacey, Meredith Fairbanks, David Tennant, Cameron Weeks, Richard Quinlan, Alexandra Edward, Chris Carter, Doug Hamilton, Peter Tozer, Renaye Horne, Tracey Gianatti, Paul Carmody, Ian Foster, Michele John, Ross George, Imma Farré, Ian Kininmonth, Dennis Van Gool, Neil Coles, Bill Porter, Louise Barton, Richard Harper, Peter Ritson, Tony Beck, Chris Mitchell, Michael Hill, Fiona Barker-Reid, Will Gates, Ken Wilson, Rob Baigent, Ian Galbally, Mick Meyer, Ian Weeks, Traci Griffin, D. Rodriguez, M. Probust, M. Meyers, D. Chen, A. Bennett, W. Strong, R. Nussey, I Galbally, M. Howden

Crop Updates

This session covers forty four papers from different authors:

PLENARY

1. 2005 Outlook, David Stephens and Nicola Telcik, Department of Agriculture

FERTILITY AND NUTRITION

2. The effect of higher nitrogen fertiliser prices on rotation and fertiliser strategies in cropping systems, Ross Kingwell, Department of Agriculture and University of Western Australia

3. Stubble management: The short and long term implications for crop nutrition and soil fertility, Wayne Pluske, Nutrient Management Systems and Bill Bowden, Department of Agriculture

4. Stubble management: The pros and cons of different methods, Bill Bowden, Department of Agriculture, Western Australia and Mike Collins, …

Dna Molecules And Polypeptides Of Pseudomonas Siringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 6,852,835 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski

Department of Plant Pathology: Faculty Publications

One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can …

Polarisome Meets Spitzenkö̈Rper: Microscopy, Genetics, And Genomics Converge, Steven D. Harris, Nick D. Read, Robert W. Roberson, Brian Shaw, Stephan Seiler, Mike Plamann, Michelle Momany

Department of Plant Pathology: Faculty Publications

The impact of filamentous fungi on human welfare has never been greater. Fungi are acknowledged as the most economically devastating plant pathogens (1) and are attaining increasing notoriety for their ability to cause life-threatening infections in humans (57, 71), and fungal products sustain a billion dollar manufacturing industry (70). The tools available to study filamentous fungi are more sophisticated than ever and include the complete annotated genome sequences of multiple filamentous fungi (12), resources being made available through various functional genomics projects, and advanced bioimaging methods, including high-resolution live-cell imaging (20, 32) and electron tomography (19, 50). The increasing impact …

Mutual Interference Between Genomic Rna Replication And Subgenomic Mrna Transcription In Brome Mosaic Virus, Valery Z. Grdzelishvili, Hernan Garcia-Ruiz, Tokiko Watanabe, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane- associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for genomic RNA3 and sgRNA syntheses. To begin elucidating their relations, we examined the interaction of RNA3 replication and sgRNA transcription in Saccharomyces cerevisiae expressing 1a and 2a, which support the full RNA3 replication cycle. Blocking sgRNA transcription stimulated RNA3 replication by up to 350%, implying that sgRNA transcription inhibits RNA3 replication. Such inhibition was independent of the …

No Political Interference In Us Agricultural Grants, Anne Vidaver

Department of Plant Pathology: Faculty Publications

I write on behalf of several former chief scientists in charge of the US Department of Agriculture’s National Research Initiative (NRI) competitive grants program. We wish to clarify part of your Editorial “A chance for growth” (Nature 432, 257; 2004). The Editorial could be interpreted as suggesting that the department’s competitive peer-reviewed research programs are influenced by political interests. Such interests have played a part in dictating the general areas in which to conduct research, but as chief scientists in the competitive programs area, we did not observe interference with the peer-review process itself.

The Glycosphingolipid Globotriaosylceramide In The Metastatic Transformation Of Colon Cancer, Olga Kovbasnjuk, Rakhilya Mourtazina, Boris Baibakov, Thomas Wang, Christian Elowsky, Michael A. Choti, Anne Kane, Mark Donowitz

Department of Plant Pathology: Faculty Publications

The most devastating aspect of cancer is the emergence of metastases. Thus, identification of potentially metastatic cells among a tumor cell population and the underlying molecular changes that switch cells to a metastatic state are among the most important issues in cancer biology. Here we show that, although normal human colonic epithelial cells lack the glycosphingolipid globotriaosylceramide (Gb₃), this molecule is highly expressed in metastatic colon cancer. In addition, a subpopulation of cells that are greatly enriched in Gb₃ and have an invasive phenotype was identified in human colon cancer cell lines. In epithelial cells in culture, …

The Hrpk Operon Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes Two Proteins Secreted By The Type Iii (Hrp) Protein Secretion System: Hopb1 And Hrpk, A Putative Type Iii Translocator, Tanja Petnicki-Ocwieja, Karin V. Van Dijk, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant cells for pathogenicity. The P. syringae TTSS is encoded by hrp-hrc genes that reside in a central region of a pathogenicity island (Pai). Flanking one side of this Pai is the exchangeable effector locus (EEL). We characterized the transcriptional expression of the open reading frames (ORFs) within the EEL of P. syringae pv. tomato DC3000. One of these ORFs, PSPTO1406 (hopB1) is expressed in the same transcriptional unit as hrpK. …

The Structure Of A T=169d Algal Virus, Pbcv-1, At 15Å Resolution, X. Yan, V. Bowman, N. H. Olson, James Gurnon, James L. Van Etten, Michael G. Rossmann, T. S. Baker

James Van Etten Publications

Paramecium bursaria chlorella virus 1 (PBCV-1) (genus Chlorovirus, family Phycodnaviridae), infects certain unicellular, exsymbiotic, chlorella-like green algae [1]. The PBCV-1 virion (~1x109 Da) has a linear dsDNA genome (330kbp) and contains at least 100 different proteins [1, unpublished data]. A published cryo-electron microscopy and three-dimensional (3D) image reconstruction of PBCV-1 (26Å resolution) revealed that the outer icosahedral glycoprotein capsid, with a maximum diameter of 1,900Å along the five-fold axis, consists of 1680 trimeric capsomers (trimer) and 12 pentameric capsomers (pentamer) [2]. These capsomers are arranged on a T=169d (h=7, k=8) quasi-equivalent lattice. The trimers and pentamers are organized into 20 …

Functional Implication Of The Trna Genes Encoded In The Chlorella Virus Pbcv-L Genome, Da Young Lee, Michael V. Graves, James L. Van Etten, Tae-Jin Choi

James Van Etten Publications

The prototype Chlorella virus PBCV-l encodes 11 tRNA genes and over 350 protein-encoding genes in its 330 kbp genome. Initial attempts to overexpress the recombinant A189/192R protein, a putative virus attachment protein, in E. coli strain BL21(DE3) SI were unsuccessful, and multiple protein bands were detected on Western blots. However, the full-length A189/192R recombinant protein or fragments derived from it were detected when they were expressed in E. coli BL21 CodonPlus (DE3) RIL, which contains extra tRNAs. Codon usage analysis of the a1891192r gene showed highly biased usage of the AGA and AUA codons compared to genes encoded by E. …

Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins

Department of Plant Pathology: Faculty Publications

A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil samples. No regulated pest species were recovered during this survey. Meloidogyne juveniles extracted from soil were identified by mitochondrial and 18S ribosomal molecular markers. Eighty-two DNA sequences representing the two marker regions for Meloidogyne species were submitted to GenBank to facilitate evaluation of marker variability. Sufficient 18S variation was observed among some Meloidogyne species to aid in identification; however, nucleotide sequence from …

Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Taxonomy: Chlorella viruses are assigned to the family Phycodnaviridae, genus Chlorovirus, and are divided into three species: Chlorella NC64A viruses, Chlorella Pbi viruses and Hydra viridis Chlorella viruses. Chlorella viruses are large, icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorella-like green algae. The type member is Paramecium bursaria chlorella virus 1 (PBCV-1).

Physical properties: Chlorella virus particles are large (molecular weight ~1 × 10⁹ Da) and complex. The virion of PBCV-1 contains more than 100 different proteins; the major capsid protein, Vp54, comprises ~40% of the virus protein. Cryoelectron microscopy and three-dimensional image reconstruction of PBCV-1 virions …

Proline Suppresses Apoptosis In The Fungal Pathogen Colletotrichum Trifolii, Changbin Chen, Martin B. Dickman

Department of Plant Pathology: Faculty Publications

The role of reactive oxygen species (ROS) in cell communication, control of gene expression, and oxygen sensing is well established. Inappropriate regulation of ROS levels can damage cells, resulting in a diseased state. In Colletotrichum trifolii, a fungal pathogen of alfalfa, the mutationally activated oncogenic fungal Ras (DARas) elevates levels of ROS, causing abnormal fungal growth and development and eventual apoptotic-like cell death but only when grown under nutrient-limiting conditions. Remarkably, restoration to the wild-type phenotype requires only proline. Here, we describe a generally unrecognized function of proline: its ability to function as a potent antioxidant and inhibitor of …

Genome Sequences Of Agropyron Mosaic Virus And Hordeum Mosaic Virus Support Reciprocal Monophyly Of The Genera Potyvirus And Rymovirus In The Family Potyviridae, Roy C. French, D.C. Stenger

Department of Plant Pathology: Faculty Publications

Assignment of mite-transmitted species to the genus Rymovirus (family Potyviridae) has changed several times, and the status of the genus has been questioned. To address this issue, complete genome sequences of the rymoviruses Agropyron mosaic virus (AgMV) and Hordeum mosaic virus (HoMV) were determined. AgMV (9540 nucleotides) and HoMV (9463 nucleotides) each encode a single polyprotein with proteinase cleavage sites demarcating protein products characteristic of monopartite species of the family Potyviridae. Of the described species of Potyviridae, AgMV and HoMV are most closely related to each other (68.5% nucleotide and 71.6% amino acid sequence identity) and equidistant …

Morphogenesis In Germinating Fusarium Graminearum Macroconidia, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Fusarium graminearum (teleomorph Gibberella zeae) is a significant pathogen of wheat and corn. F. graminearum forms multicellular macroconidia that play an important role in dissemination of the disease. The spatial pattern of morphogenesis in germinating macroconidia is described. Germ tubes preferentially emerge from the apical cells in a bipolar pattern that appears to be common to filamentous fungi. Chitin deposition occurs at two locations: the spore apices and cortical regions of macroconidial cells that subsequently produce a germ tube. The spatial pattern of morphogenesis requires the presence of functional microtubules, which may be responsible for the transport of key …

Fundamental Contribution Of Β-Oxidation To Polyketide Mycotoxin Production In Planta, Lori A. Maggio-Hall, Richard A. Wilson, Nancy P. Keller

Fungal Molecular Plant-Microbe Interactions

Seed contamination with polyketide mycotoxins, including aflatoxin (AF) and sterigmatocystin (ST) produced by Aspergillus spp., is an agricultural, economic, and medical issue worldwide. Acetyl-CoA, the fundamental building block of all known fungal polyketides, is generated by a large number of biochemical pathways, including β-oxidation of fatty acids and glycolysis of sugars. We present several lines of evidence to support a major role for seed fatty acids in formation of AF and ST in A. flavus, A. parasiticus, and A. nidulans. Aspergillus strains exhibiting canonical signs of oleic acid–induced peroxisome proliferation, including increased catalase activity, β-oxidation gene expression, and …

Chlorella Virus Marburg Topoisomerase Ii: High Dna Cleavage Activity As A Characteristic Of Chlorella Virus Type Ii Enzymes, Jennifer S. Dickey, Tae-Jin Choi, James L. Van Etten, Neil Osheroff

Department of Plant Pathology: Faculty Publications

Although the formation of a covalent enzyme-cleaved DNA complex is a prerequisite for the essential functions of topoisomerase II, this reaction intermediate has the potential to destabilize the genome. Consequently, all known eukaryotic type II enzymes maintain this complex at a low steady-state level. Recently, however, a novel topoisomerase II was discovered in Paramecium bursaria chlorella virus-1 (PBCV-1) that has an exceptionally high DNA cleavage activity [Fortune et al. (2001) J. Biol. Chem. 276, 24401-24408]. If robust DNA cleavage is critical to the physiological functions of chlorella virus topoisomerase II, then this remarkable characteristic should be …

Expression Of The Tobacco Β- 1,3-Glucanase Gene, Pr-2d, Following Induction Of Sar With Peronospora Tabacina, Deanna L. Funnell, Christopher B. Lawrence, Jeffrey F. Pedersen, Christopher L. Schardl

Department of Plant Pathology: Faculty Publications

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic P-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to …