Life Sciences Commons^™

Virion Composition Of Wheat Streak Mosaic Virus (Wsmv) And Its Use As An Expression Vector, Sourav Pal

Department of Plant Pathology: Dissertations, Theses, and Student Research

Wheat (Triticum aestivum L.) is the principal cereal grown in the United States, whose production ranks third in the world. Bacterial leaf streak (BLS) disease caused by Xanthomonas translucens pv. undulosa (xtu) is an emerging threat to wheat production in the Great Plains region of the United States of America. In this study, we engineered sequences encoding several plant-based antimicrobial peptides (AMP) were engineered into the WSMV genome and examined their effect on BLS disease in wheat infected by WSMV harboring AMP sequences. Out of nine AMPs, wheat expressing Ace-AMP1, PDF13, TK-AMP-D1, and TK-AMP-D3 produced resistance against the …

B.R. Wells Arkansas Rice Research Studies 2023, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 49.0% of total U.S. rice production and 49.6% of the total acres planted to rice in 2023. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

Arkansas Corn And Grain Sorghum Research Studies 2023, Jason Kelley, Travis Faske

Arkansas Agricultural Experiment Station Research Series

e 2023 edition of the Arkansas Corn and Grain Sorghum Research Studies Series includes research results on topics pertaining to corn and grain sorghum production, including weed, disease, and insect management; economics; irrigation; agronomics; soil fertility; mycotoxins; cover crop management; and research verification program results. Our objective is to capture and broadly distribute the results of research projects funded by the Arkansas Corn and Grain Sorghum Board. The intended audience includes producers and their advisors, current investigators, and future researchers. The Series serves as a citable archive of research results.

Assessment Of Foam Decontamination In Tomato Washing Process, Basim M. Alohali

Dissertations and Doctoral Documents from University of Nebraska-Lincoln, 2023–

The work of the dissertation investigated the efficacy of foam-based decontamination treatments to enhance microbial safety in Roma tomatoes, specifically targeting natural microflora and Salmonella contamination. Growing concerns over foodborne illnesses linked to fresh tomatoes and the limitations of conventional decontamination techniques, such as organic matter built-up and limited microbial reduction, are addressed. The primary objectives were to characterize the physiochemical properties of foams formed using various concentrations of Tween 20 (T20) and sodium dodecyl sulfate (SDS) with and without the addition of sodium hypochlorite (NaOCl) and peracetic acid (PAA), compare the efficacy of foam and traditional dump tank (DT) …

Post-Translational Regulation Of A Bidomain Glycerol-3-Phosphate Dehydrogenase Catalyzing Glycerol Synthesis Under Salinity Stress In Chlamydomonas Reinhardtii, Itzela Cruz-Powell, Binita Subedi, Yeongho Kim, Daniela Morales-Sánchez, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty and Staff Publications

Core chlorophytes possess glycerol-3-phosphate dehydrogenases (GPDs) with an unusual bidomain structure, consisting of a glycerol-3-phosphate phosphatase (GPP) domain fused to canonical GPD domains. These plastid-localized enzymes have been implicated in stress responses, being required for the synthesis of glycerol under high salinity and triacylglycerols under nutrient deprivation. However, their regulation under varying environmental conditions is poorly understood. C. reinhardtii transgenic strains expressing constitutively bidomain GPD2 did not accumulate glycerol or triacylglycerols in the absence of any environmental stress. Although the glycerol contents of both wild type and transgenic strains increased significantly upon exposure to high salinity, cycloheximide, an inhibitor of …

Undergraduate Instruction In Plant Pathology: In-Class Group Activities Improve Teamwork Skills In Semester-Long Projects, Teddy Garcia-Aroca

UNL Faculty Course Portfolios

PLPT 210: Plant Pathogens and Disease is an introductory-level undergraduate course offered by the Department of Plant Pathology at University of Nebraska-Lincoln. The course covers the basic principles of plant pathology, fundamental aspects of pathogen biology, and common management strategies. The course is offered every spring, and the enrollment is ~40 students per semester. Most students enrolled in the course are sophomores, juniors, to a lesser extent seniors from the Agronomy and Horticulture majors, Plant and Landscapes Systems, and Environmental Sciences. The course consists of lectures and in-class activities, and students are evaluated through quizzes, individual and in-group homework assignments, …

Genomes Of Multicellular Algal Sisters To Land Plants Illuminate Signaling Network Evolution, Xuehuan Feng, Jinfang Zheng, Iker Irisarri, Huihui Yu, Bo Zheng, Zahin Ali, Sophie De Vries, Jean Keller, Janine M. R. Fürst-Jansen, Armin Dadras, Jaccoline M. S. Zegers, Tim P. Rieseberg, Amra Dhabalia Ashok, Tatyana Darienko, Maaike J. Bierenbroodspot, Lydia Gramzow, Romy Petroll, Fabian B. Haas, Noe Fernandez-Pozo, Orestis Nousias, Tang Li, Elisabeth Fitzek, W. Scott Grayburn, Nina Rittmeier, Charlotte Permann, Florian Rümpler, John M. Archibald, Günter Theißen, Jeffrey P. Mower, Maike Lorenz16, Henrik Buschmann, Klaus Von Schwartzenberg, Lori Boston, Richard D. Hayes, Chris Daum, Kerrie Barry, Igor V. Grigoriev, Xiyin Wang, Fay-Wei Li, Stefan A. Rensing, Julius Ben Ari, Noa Keren, Assaf Mosquna, Andreas Holzinger, Pierre-Marc Delaux, Chi Zhang, Jinling Huang, Marek Mutwil6,, Jan De Vries, Yanbin Yin

Center for Plant Science Innovation: Faculty and Staff Publications

Zygnematophyceae are the algal sisters of land plants. Here we sequenced four genomes of filamentous Zygnematophyceae, including chromosome-scale assemblies for three strains of Zygnema circumcarinatum. We inferred traits in the ancestor of Zygnematophyceae and land plants that might have ushered in the conquest of land by plants: expanded genes for signaling cascades, environmental response, and multicellular growth. Zygnematophyceae and land plants share all the major enzymes for cell wall synthesis and remodifications, and gene gains shaped this toolkit. Co-expression network analyses uncover gene cohorts that unite environmental signaling with multicellular developmental programs. Our data shed light on a molecular …

Momkk1 And Moatg1 Dichotomously Regulating Autophagy And Pathogenicity Through Moatg9 Phosphorylation In Magnaporthe Oryzae, Yun Kong, Pusheng Guo, Jiayun Xu, Jiaxu Li, Miao Wu, Ziqi Zhang, Yifan Wang, Xinyu Liu, Leiyun Yang, Muxing Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang

School of Medicine Faculty Publications

Autophagy is a central biodegradation pathway critical in eliminating intracellular cargo to maintain cellular homeostasis and improve stress resistance. At the same time, the key component of the mitogen-activated protein kinase cascade regulating cell wall integrity signaling MoMkk1 has an essential role in the autophagy of the rice blast fungus Magnaporthe oryzae. Still, the mechanism of how MoMkk1 regulates autophagy is unclear. Interestingly, we found that MoMkk1 regulates the autophagy protein MoAtg9 through phosphorylation. MoAtg9 is a transmembrane protein subjected to phosphorylation by autophagy-related protein kinase MoAtg1. Here, we provide evidence demonstrating that MoMkk1-dependent MoAtg9 phosphorylation is required for phospholipid …

Up-Regulation Of Non-Photochemical Quenching Improves Water Use Efficiency And Reduces Whole-Plant Water Consumption Under Drought In Nicotiana Tabacum, Benjamin Turc, Seema Sahay, Jared Haupt, Talles De Oliveira Santos, Geng Bai, Katarzyna Glowacka

Center for Plant Science Innovation: Faculty and Staff Publications

Water supply limitations will likely impose increasing restrictions on future crop production, underlining a need for crops that use less water per mass of yield. Water use efficiency (WUE) therefore becomes a key consideration in developing resilient and productive crops. In this study, we hypothesized that it is possible to improve WUE under drought conditions via modulation of chloroplast signals for stomatal opening by up-regulation of non-photochemical quenching (NPQ). Nicotiana tabacum plants with strong overexpression of the PsbS gene encoding PHOTOSYSTEM II SUBUNIT S, a key protein in NPQ, were grown under differing levels of drought. The PsbS-overexpressing lines lost …

The Emerging Role Of Non-Coding Rnas (Ncrnas) In Plant Growth, Development, And Stress Response Signaling, Amit Yadav, Jyotirmaya Mathan, Arvind Kumar Dubey, Anuradha Singh

Center for Plant Science Innovation: Faculty and Staff Publications

Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play a crucial regulatory role in plant biology, surpassing the essential functions of RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent an emerging class of regulators, operating directly in the form of small interfering RNAs (siRNAs), microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). These ncRNAs exert control at various levels, including transcription, posttranscription, translation, and epigenetic. Furthermore, they interact with each other, contributing to a variety of biological processes and mechanisms associated with stress resilience. …

Autophagy And Cell Wall Integrity Pathways Coordinately Regulate The Development And Pathogenicity Through Moatg4 Phosphorylation In Magnaporthe Oryzae, Pusheng Guo, Yurong Wang, Jiayun Xu, Zhixiang Yang, Ziqi Zhang, Jinyi Qian, Jiexiong Hu, Ziyi Yin, Leiyun Yang, Muxing Liu, Xinyu Liu, Gang Li, Haifeng Zhang, Ryan Rumsey, Ping Wang, Zhengguang Zhang

School of Medicine Faculty Publications

Autophagy and Cell wall integrity (CWI) signaling are critical stress-responsive processes during fungal infection of host plants. In the rice blast fungus Magnaporthe oryzae, autophagy-related (ATG) proteins phosphorylate CWI kinases to regulate virulence; however, how autophagy interplays with CWI signaling to coordinate such regulation remains unknown. Here, we have identified the phosphorylation of ATG protein MoAtg4 as an important process in the coordination between autophagy and CWI in M. oryzae. The ATG kinase MoAtg1 phosphorylates MoAtg4 to inhibit the deconjugation and recycling of the key ATG protein MoAtg8. At the same time, MoMkk1, a core kinase of CWI, also phosphorylates …

Watch Out For Putnam Scale, Diaspidiotus Ancylus, Alfred Daniel Johnson, Muhammad Ahmed,, Karla . M. Addesso

Extension Publications

No abstract provided.

Vascular Streak Dieback An Emerging Threat To The Redbud Nursery Production In The Southeastern United States, Fulya Baysal-Gurel, Prabha Liyanapathiranage

Extension Publications

No abstract provided.

Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips, Salomón Aguilar, David Allen, Alfonso Alonso, Kristina J. Anderson-Teixeira, Matthew E. Baker, Jennifer L. Baltzer, Pulchérie Bissiengou, Mariana Bonfim, Norman A. Bourg, Warren Y. Brockelman, David F.R.P. Burslem, Li Wan Chang, Yang Chen, Jyh Min Chiang, Chengjin Chu, Keith Clay, Susan Cordell, Mary Cortese, Jan Den Ouden, Christopher Dick, Sisira Ediriweera, Erle C. Ellis, Anna Feistner, Amy L. Freestone, Thomas Giambelluca, Christian P. Giardina, Gregory S. Gilbert, Fangliang He, Jan Holík, Robert W. Howe, Walter Huaraca Huasca, Stephen P. Hubbell, Faith Inman, Patrick A. Jansen, Daniel J. Johnson, Kamil Kral, Sabrina E. Russo, Et Al.

Center for Plant Science Innovation: Faculty and Staff Publications

One mechanism proposed to explain high species diversity in tropical systems is strong negative conspecific density dependence (CDD), which reduces recruitment of juveniles in proximity to conspecific adult plants. Although evidence shows that plant-specific soil pathogens can drive negative CDD, trees also form key mutualisms with mycorrhizal fungi, which may counteract these effects. Across 43 large-scale forest plots worldwide, we tested whether ectomycorrhizal tree species exhibit weaker negative CDD than arbuscular mycorrhizal tree species. We further tested for conmycorrhizal density dependence (CMDD) to test for benefit from shared mutualists. We found that the strength of CDD varies systematically with mycorrhizal …

A Global-Temporal Analysis On Phytophthora Sojae Resistance-Gene Efficacy, Austin G. Mccoy, Richard R. Belanger, Carl A. Bradley, Daniel G. Cerritos-Garcia, Vinicius C. Garnica, Loren Giesler, Pablo E. Grijalba, Eduardo Guillin, Maria A. Henriquez, Yong Min Kim, Dean K. Malvick, Rashelle L. Matthiesen, Santiago X. Mideros, Zachary A. Noel, Alison E. Robertson, Mitchell G. Roth, Clarice L. Schmidt, Damon L. Smith, Adam H. Sparks, Darcy E.P. Telenko, Vanessa Tremblay, Owen Wally, Martin I. Chilvers

Department of Plant Pathology: Faculty Publications

Plant disease resistance genes are widely used in agriculture to reduce disease outbreaks and epidemics and ensure global food security. In soybean, Rps (Resistance to Phytophthora sojae) genes are used to manage Phytophthora sojae, a major oomycete pathogen that causes Phytophthora stem and root rot (PRR) worldwide. This study aims to identify temporal changes in P. sojae pathotype complexity, diversity, and Rps gene efficacy. Pathotype data was collected from 5121 isolates of P. sojae, derived from 29 surveys conducted between 1990 and 2019 across the United States, Argentina, Canada, and China. This systematic review shows a loss of efficacy of …

Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The benefit of unmanned aircraft systems and image processing methods in agronomic research across numerous crops has been well documented as has the importance of wheat, Triticum aestivum L., on the global food supply. Hence there is great interest in digital solutions applied to aspects of wheat breeding. A major trait of importance to winter wheat breeders in higher latitudes is winter survival, which can result in poor yield and performance if lines do not survive extreme cold. Scoring winter survival is most commonly based on visual score of 0% to 100% with the higher percentage conveying higher winter survival …

Arkansas Soybean Research Studies 2022, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2022 when compared to the other soybean-producing states in the U.S. The state represented 3.04% of the total U.S. soybean production and 3.64% of the total acres planted in soybean in 2022. The 2022 state soybean average yield was 52.0 bushels per acre, tying the previous state yield record of 52 bushels per acre set in 2021. The top five soybean-producing counties in 2022 were Mississippi, Crittenden, Phillips, Poinsett, and Arkansas (Table 1). These five counties accounted for over 35.7% of the …

The Pseudomonas Syringae Pv. Tomato Dc3000 Effector Hopd1 Interferes With Cellular Dynamics Associated With The Function Of The Plant Immune Protein Atnhr2b, Luis Francisco Marín-Ponce, Catalina Rodríguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas

Department of Plant Pathology: Faculty Publications

The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes disease in tomato, in the model plant Arabidopsis thaliana, and conditionally in Nicotiana benthamiana. The pathogenicity of Pst DC3000 is mostly due to bacterial virulence proteins, known as effectors, that are translocated into the plant cytoplasm through the type III secretion system (T3SS). Bacterial type III secreted effectors (T3SEs) target plants physiological processes and suppress defense responses to enable and support bacterial proliferation. The Pst DC3000 T3SE HopD1 interferes with plant defense responses by targeting the transcription factor NTL9. This work shows that HopD1 also targets …

Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty and Staff Publications

MicroRNAs ( miRNAs ) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex ( MC ) . Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 ( DCL1 ) and SERRATE ( SE ) and directly binds the stem- loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript ( pri-miRNAs ) with MC are reduced. …

Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Crop Variety Improvement Program.

The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests were conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location and cultural practice information accompany each …

Dynamic Reconfiguration Of Switchgrass Proteomes In Response To Rust (Puccinia Novopanici) Infection, Nathan A. Palmer, Sophie Alvarez, Michael J. Naldrett, Anthony Muhle, Gautam Sarath, Serge J. Edmé, Satyanarayana Tatineni, Robert B. Mitchell, Gary Yuen

Department of Plant Pathology: Faculty Publications

Switchgrass (Panicum virgatum L.) can be infected by the rust pathogen (Puccinia novopanici) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 …

Overcoming Genetic Paucity Of Camelina Sativa: Possibilities For Interspecific Hybridization Conditioned By The Genus Evolution Pathway, Rostyslav Y. Blume, Ruslan Kalendar, Liang Guo, Edgar B. Cahoon, Yaroslav B. Blume

Center for Plant Science Innovation: Faculty and Staff Publications

Camelina or false flax (Camelina sativa) is an emerging oilseed crop and a feedstock for biofuel production. This species is believed to originate from Western Asian and Eastern European regions, where the center of diversity of the Camelina genus is located. Cultivated Camelina species arose via a series of polyploidization events, serving as bottlenecks narrowing genetic diversity of the species. The genetic paucity of C. sativa is foreseen as the most crucial limitation for successful breeding and improvement of this crop. A potential solution to this challenge could be gene introgression from Camelina wild species or from …

A Facile Agrobacterium‑Mediated Transformation Method For The Model Unicellular Green Algae Chlamydomonas Reinhardtii, Truyen N. Quach, Shirley J. Sato, Mark R. Behrens, Paul N. Black, Concetta C. Dirusso, Heriberto D. Cerutti, Tom Elmo Clemente

Center for Plant Science Innovation: Faculty and Staff Publications

A reliable and simple Agrobacterium-mediated transformation system for the unicellular green algae model organism Chlamydomonas reinhardtii has been developed. The protocol has been successfully employed with both neomycin phosphotransferase II (nptII) and the phleomycin resistance (bleI) genes coupled with the selective agents paromomycin and zeocin, respectively. A set of binary vectors were assembled that carry the selectable marker cassettes under control either of the Rbcs2 alone or fused to the HSP270A leader sequence, PsaD, or ß-tubulin2 promoters. The corresponding T-DNA elements also harbored a cassette with a codon-optimized version of yellow fluorescence protein (YFP) under …

Methionine Biosynthesis Enzyme Momet2 Is Required For Rice Blast Fungus Pathogenicity By Promoting Virulence Gene Expression Via Reducing 5mc Modification, Huimin Li, Pengcheng Mo, Jun Zhang, Zhuoer Xie, Xinyu Liu, Han Chen, Leiyun Yang, Muxing Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang

School of Medicine Faculty Publications

The emergence of fungicide resistance severely threatens crop production by limiting the availability and application of established fungicides. Therefore, it is urgent to identify new fungicidal targets for controlling plant diseases. Here, we characterized the function of a conserved homoserine O-acetyltransferase (HOA) from the rice blast fungus Magnaporthe oryzae that could serve as the candidate antifungal target. Deletion of the MoMET2 and MoCYS2 genes encoding HOAs perturbed the biosynthesis of methionine and S-adenyl methionine, a methyl group donor for epigenetic modifications, and severely attenuated the development and virulence of M. oryzae. The ΔMomet2 mutant is significantly increased in 5-methylcytosine (5mC) …

Genetic Improvement Of Tocotrienol Content Enhances The Oxidative Stability Of Canola Oil, Min Deng, Hao Chen, Wei Zhang, Edgar B. Cahoon, Yongming Zhou, Chunyu Zhang

Center for Plant Science Innovation: Faculty and Staff Publications

Background: Tocotrienols and tocopherols, which are synthesized in plastids of plant cells with similar functionalities, comprise vitamin E to serve as a potent lipid-soluble antioxidant in plants. The synthesis of tocopherols involves the condensation of homogentisic acid (HGA) and phytyl diphosphate (PDP) under the catalysis of homogentisate phytyltransferase (HPT). Tocotrienol synthesis is initiated by the condensation of HGA and geranylgeranyl diphosphate (GGDP) mediated by homogentisate geranylgeranyl transferase (HGGT). As one of the most important oil crops, canola seed is regarded as an ideal plant to efficiently improve the production of vitamin E tocochromanols through genetic engineering approaches. However, only …

B. R. Wells Arkansas Rice Research Studies 2022, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 50.1% of total U.S. rice production and 49.8% of the total acres planted to rice in 2022. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

Arkansas Corn And Grain Sorghum Research Studies 2022, Victor Ford, Jason Kelley, Nathan Mckinney Ii

Arkansas Agricultural Experiment Station Research Series

The 2022 edition of the Arkansas Corn and Grain Sorghum Research Studies Series includes research results on topics pertaining to corn and grain sorghum production, including weed, disease, and insect management; economics; sustainability; irrigation; post-harvest drying; soil fertility; mycotoxins; cover crop management; and research verification program results. Our objective is to capture and broadly distribute the results of research projects funded by the Arkansas Corn and Grain Sorghum Board. The intended audience includes producers and their advisors, current investigators, and future researchers. The Series serves as a citable archive of research results.

Reports in this publication are 2–3 year summaries. …