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Articles 91 - 120 of 366
Full-Text Articles in Biomedical Engineering and Bioengineering
Preparation Of Dual Fluorescence Agents-Loaded Nanoparticle And Its In Vitro And In Vivo Application, Hong-Hao Thi Vu
Preparation Of Dual Fluorescence Agents-Loaded Nanoparticle And Its In Vitro And In Vivo Application, Hong-Hao Thi Vu
Bioengineering Theses - Archive
There’re many existing imaging tools that composed of different imaging moieties to improve both resolution and sensitivity, however there’s not yet any imaging method that could be effectively utilized in both in vitro and in vivo study. To fill the gap, dual dyes-labeling fluorescent imaging nanoprobes was synthesized to simultaneous carry both visible fluorescent isothiocyanate (FITC) dye and near infra red (NIR) dye, 1,1′,3,3,3′,3′- hexamethylindotricarbocyanine iodide dye agent (HITC). For that, nanoparticles (NPs) was synthesized from 2-Hydroxyethyl methacrylate, styrene, acrylic acid and fluorescein-o-acrylate which contains visible FITC dye. The NPs were then labeled with HITC dye via physical entrapment to …
Detection Of Molecular Biomarkers Involved In The Induction Of Emt, Migration And Invasion, And Drug Resistance In Breast Cancer Cells In 3d Confinement, Tamara Nicole Hill
Detection Of Molecular Biomarkers Involved In The Induction Of Emt, Migration And Invasion, And Drug Resistance In Breast Cancer Cells In 3d Confinement, Tamara Nicole Hill
Bioengineering Dissertations - Archive
Standard-of-care regimes for cancer treatment have become more effective and sophisticated over the last decade due to research developments which facilitate specific targeting of treatments to certain cancers, and the ability to provide specialized care to cancer patients. However, cancer treatments overall still fall short of preventing spread of disease from the primary tumor to secondary sites where they settle and form new more aggressive tumors. It is this stage of disease progression which results in the majority of cancer deaths. The long-term goal of this research is to develop a system for the effective high throughput screening of chemotherapeutic …
Fabrication And In Vitro Characterization Of An Implant For The Detection Of Metastatic Cancer Cells, Carlos Martin Cantu
Fabrication And In Vitro Characterization Of An Implant For The Detection Of Metastatic Cancer Cells, Carlos Martin Cantu
Bioengineering Theses - Archive
Greater than 90% of cancer related deaths are due to metastasis (Hayes & Wicha, 2011). Since current conventional techniques detect the disease by the time that it has become systemic, our lab has developed a hydrogel based cancer trap that can attract metastatic cancer cells via cytokine release. However, for continual monitoring and accessibility to the gel, an implant is needed for delivery, retention, and retrieval of the gel. The research undergone in this thesis seeks to develop such a method. Namely, the objective was to develop a polylactide (PLA) based implant that is capable of delivering and retaining a …
Development Of Mussel Inspired Nanocomposite Adhesives For Biomedical Applications, Nikhil Pandey
Development Of Mussel Inspired Nanocomposite Adhesives For Biomedical Applications, Nikhil Pandey
Bioengineering Dissertations - Archive
Popular bioadhesives, such as fibrin, cyanoacrylate and albumin–glutaraldehyde-based materials, have been applied for clinical applications in wound healing, drug delivery, and bone and soft tissue engineering; however, their performances are limited by weak adhesion strength and rapid degradation. The long-term goal of this research is to develop a strong tissue adhering nanocomposite for tissue interfacing and wound healing applications. We begin by developing a mussel-inspired, nanocomposite–based, biodegradable tissue adhesive by blending poly (lactic-co-glycolic acid) (PLGA) or N-hydroxysuccinimide modified PLGA nanoparticles (PLGA-NHS) and polydopamine nanoparticles with mussel-inspired polymers. Adhesive strength measurement of the nanocomposites on porcine skin-muscle constructs revealed that the …
Nanoparticles For Gene Therapy And Protein Delivery To Induce Angiogenesis As An Alternative Treatment For Peripheral Arterial Disease, Linda Christellia Noukeu
Nanoparticles For Gene Therapy And Protein Delivery To Induce Angiogenesis As An Alternative Treatment For Peripheral Arterial Disease, Linda Christellia Noukeu
Bioengineering Dissertations - Archive
Peripheral arterial disease (PAD) is defined as a slow, progressive disorder of the lower extremity arterial vessels characterized by chronic narrowing that often results in occlusion and is associated with loss of functional capacity. Although the PAD occurrence rate is increasing in the elderly population, outcomes with current treatment strategies are suboptimal. Hence, there is an urgent need to develop new technologies that overcome limitations of traditional modalities for PAD detection and therapy. In this research, nanoparticles have been developed and used as carriers for various therapeutic agents to treat PAD. In particular, via protein/gene delivery, we developed and formulated …
An Enhancement Of Light Reflectance Spectroscopy As A Modality For The Detection Of Positive Surgical Margins In Radical Prostatectomy, Zengxing Pang
An Enhancement Of Light Reflectance Spectroscopy As A Modality For The Detection Of Positive Surgical Margins In Radical Prostatectomy, Zengxing Pang
Bioengineering Theses - Archive
Light Reflectance Spectroscopy (LRS) is an imaging method that has shown increasingly promising evidence in its ability to detect various forms of cancer tissues. Our previous studies have illustrated the LRS modality’s capacity to differentiate normal and cancerous prostate tissues, specifically in its effectiveness in distinguishing cancer tissues classified 4+3 and 4+4 in Gleason Score. Despite having shown sufficient sensitivity and specificity towards prostate cancer detection, the pre-existing imaging system remains impractical for its intended clinical application of detecting positive surgical margins during prostatectomy. Two of the biggest challenges in the current equipment include lack of mobility and slow data …
Investigation Of Functional Connectivity And Neurovascular Coupling In Different Vigilance / Breath-Hold States Of The Brain From Simultaneous Eeg-Fnirs Measurements, Olajide Babawale
Bioengineering Dissertations - Archive
Functional connectivity and neurovascular coupling are important phenomena that occur in the brain. Functional connectivity is the study of functional brain networks and their characteristics. While neurovascular coupling is the relationship or link between neuronal activity and corresponding cerebral blood flow in the brain. Up until now, both functional connectivity and neurovascular coupling have not been studied in different vigilance states of the brain from wakeful rest state to sleep state, using simultaneous EEG-fNIRS measurements. Also, the neurovascular coupling in different states of breath-holding has not been studied using simultaneous EEG-fNIRS measurements Therefore, my doctoral research utilized a whole-head simultaneous …
Creep Response And Adhesion Dynamics Of Glioblastoma Multiforme Cells During Migration In Confined Environment, Ishan Kamal Khan
Creep Response And Adhesion Dynamics Of Glioblastoma Multiforme Cells During Migration In Confined Environment, Ishan Kamal Khan
Bioengineering Dissertations - Archive
Glioblastoma multiforme (GBM) is the most common and malignant form of primary brain tumor. One of the major challenges facing the treatment of GBM tumors is the detachment and subsequent migration of peripheral cells. Migration of GBM cells take place in mechanically distinct confined microenvironment along the borders of white matter tracts. Studies using microfluidic devices mimicking the in-vivo microenvironment have shown that the migration characteristics are significantly altered with the change in kinematic state of the migrating cell. Several studies on different types of cells have consistently shown that a cancerous cell is more compliant than a normal cell …
Investigation Of 1064-Nm Laser Fluence Within Tissue Phantoms And Its In Vivo Effects On Human Brain Networks, Hasan Parvez
Investigation Of 1064-Nm Laser Fluence Within Tissue Phantoms And Its In Vivo Effects On Human Brain Networks, Hasan Parvez
Bioengineering Theses - Archive
For the past few decades there has been an increasing interest in the usage of near infrared (NIR) laser in the medical optics field. This is due to a number of studies conducted on different animal models utilizing NIR laser, which was reported to provide improvement in such cognitive functions as memory, decision making, and executive functions. The NIR laser produces no adverse effects in the human tissue, having a potential for treating different neurological disorders. However, there is limited knowledge related to the underlying mechanism of photon interaction with the human tissue. In this thesis, I tried to address …
Revealing Hemodynamic Response And Dynamic Changes In Functional Cortical Networks Using Functional Near-Infrared Spectroscopy (Fnirs), Jianwei Cao
Bioengineering Dissertations - Archive
Therapeutic interventions, such as physical therapy and non-invasive brain electrical stimulation can enhance the physical performance of persons with brain disorders. One often-used form of physical therapy named constraint-induced movement therapy (CIMT) improves motor control skills of children with cerebral palsy (CP) through forced intensive use of the affected extremity. A complementary method to physical rehabilitation methods for overcoming a deficit is to facilitate use of the affected brain areas by non-invasive brain electrical stimulation. One currently popular form of brain stimulation is transcranial direct current stimulation (tDCS), which has been shown to enhance both motor and language performance in …
Plasma Enhanced Chemical Vapor Deposition Coating Amorphous Silica Based Implants Enhancing Angiogenesis And Mitigating Toxic Oxidative Stress In Critical Size Bone Defects, Felipe Alves Monte
Plasma Enhanced Chemical Vapor Deposition Coating Amorphous Silica Based Implants Enhancing Angiogenesis And Mitigating Toxic Oxidative Stress In Critical Size Bone Defects, Felipe Alves Monte
Bioengineering Dissertations - Archive
The advances in bone tissue engineering search for a biomaterial that could efficiently and gradually replace the bone while the new tissue grows through the implanted structure. However, due to limitations mainly related to mechanical properties and challenges, such as the balance between tissue regeneration and material degradation, the coating of well-known inert materials still has been used in orthopedics and dental clinical practices. Bone defects are commonly related to deleterious oxidative stress induced by low oxygen levels and inflammation. Toxic oxidative stress can impair damaged tissue healing due to inhibiting new blood vessels and new bone formation. The interaction …
Silica Formation With Nanofiber Morphology Via Helical Display Of The Silaffin R5 Peptide On A Filamentous Bacteriophage, In-Wong Song, Hyojung Park, Jung Han Park, Hyunook Kim, Seong Hun Kim, Justyn Jaworski, Byoung-In Sang, Sung Yi
Silica Formation With Nanofiber Morphology Via Helical Display Of The Silaffin R5 Peptide On A Filamentous Bacteriophage, In-Wong Song, Hyojung Park, Jung Han Park, Hyunook Kim, Seong Hun Kim, Justyn Jaworski, Byoung-In Sang, Sung Yi
Bioengineering Faculty Publications - Archive
Biological systems often generate unique and useful structures, which can have industrial relevance either as direct components or as an inspiration for biomimetic materials. For fabrication of nanoscale silica structures, we explored the use of the silaffin R5 peptide from Cylindrotheca fusiformis expressed on the surface of the fd bacteriophage. By utilizing the biomineralizing peptide component displayed on the bacteriophage surface, we found that low concentrations (0.09 mg/mL of the R5 bacteriophage, below the concentration range used in other studies) could be used to create silica nanofibers. An additional benefit of this approach is the ability of our R5-displaying phage …
Dual-Drug Containing Core-Shell Nanoparticles For Lung Cancer Therapy, Jyothi Unnikrishna Menon, Aneetta Kuriakose, Roshni Iyer, Elizabeth Hernandez, Leah Gandee, Shanrong Zhang, Masaya Takahashi, Zhang Zhang, Debabrata Saha, Kytai Truong Nguyen
Dual-Drug Containing Core-Shell Nanoparticles For Lung Cancer Therapy, Jyothi Unnikrishna Menon, Aneetta Kuriakose, Roshni Iyer, Elizabeth Hernandez, Leah Gandee, Shanrong Zhang, Masaya Takahashi, Zhang Zhang, Debabrata Saha, Kytai Truong Nguyen
Bioengineering Faculty Publications - Archive
Late-stage diagnosis of lung cancer occurs ~95% of the time due to late manifestation of its symptoms, necessitating rigorous treatment following diagnosis. Existing treatment methods are limited by lack of specificity, systemic toxicity, temporary remission, and radio-resistance in lung cancer cells. In this research, we have developed a folate receptor-targeting multifunctional dual drug-loaded nanoparticle (MDNP) containing a poly(N-isopropylacrylamide)-carboxymethyl chitosan shell and poly lactic-coglycolic acid (PLGA) core for enhancing localized chemo-radiotherapy to effectively treat lung cancers. The formulation provided controlled releases of the encapsulated therapeutic compounds, NU7441 - a potent radiosensitizer, and gemcitabine - an FDA approved chemotherapeutic drug for lung …
An Optical Probe For Detecting Chondrocyte Apoptosis In Response To Mechanical Injury, Yihui Huang, Jun Zhou, Amirhossein Hakamivala, Jinglei Wu, Yi Hong, Joseph Borrelli Jr., Liping Tang
An Optical Probe For Detecting Chondrocyte Apoptosis In Response To Mechanical Injury, Yihui Huang, Jun Zhou, Amirhossein Hakamivala, Jinglei Wu, Yi Hong, Joseph Borrelli Jr., Liping Tang
Bioengineering Faculty Publications - Archive
Cartilage injury induced by acute excessive contact stress is common and mostly affects young adult. Although early detection of cartilage injury may prevent serious and lifelong arthritic complications, early detection and treatment is not possible due to the lack of a reliable detection method. Since chondrocyte injury and subsequent cell death are the early signs of cartilage injury, it is likely that cartilage cell apoptosis can be used to predict the extent of injury. To test this hypothesis, a near infrared probe was fabricated to have high affinity to apoptotic cells. In vitro tests show that this apoptosis probe has …
Metabolic, Hemodynamic And Electrophysiological Effects Of Transcranial Photobiomodulation (Tpbm) On The Human Brain, Xinlong Wang
Metabolic, Hemodynamic And Electrophysiological Effects Of Transcranial Photobiomodulation (Tpbm) On The Human Brain, Xinlong Wang
Bioengineering Dissertations - Archive
Photobiomodulation (PBM) refers to the use of red-to-near-infrared light to stimulate cellular functions for physiological or clinical benefits. Transcranial photobiomodulation (tPBM) is a noninvasive form of cerebral photobiomodulation that has been observed with various improvements in human cognitive functions. The mechanism of tPBM is assumed to rely on photon absorption by cytochrome c oxidase (CCO), the terminal enzyme in the mitochondrial respiratory chain that catalyzes the reduction of oxygen for energy metabolism. However, few studies have objectively investigated hemodynamic, metabolic and electrophysiological response of the human brain to tPBM. To address this gap, my dissertation research has focused on objective …
Understanding Key Genetic Mutations And Molecular Alterations On Increasing Migration, Viability, Drug Resistance Of Malignant Brain Tumor In Confinement, Loan Thuy Bui
Bioengineering Dissertations - Archive
There are more than 120 types of primary brain and central nervous system tumors that affect people of all ages. Glioblastoma multiforme (GBM), for example, is the most frequent type of brain cancers and also among the deadliest of all cancers due to its high invasiveness and resistance to radiation and chemotherapy. Recently, most genetic mutations of malignant brain tumors have been well characterized, but the focus on migrating cancers that directly cause invasion is still in its infancy. We have learned from the literature that most glioma cells infiltrate surrounding brain tissue via by the confined tracks, and fascinated …
Naturally-Derived Extracellular Matrix Materials For Meniscus Repair And Regeneration, Jinglei Wu
Naturally-Derived Extracellular Matrix Materials For Meniscus Repair And Regeneration, Jinglei Wu
Bioengineering Dissertations - Archive
Human meniscus plays critical roles in a knee joint by transmitting weight-bearing force, absorbing shock, and stabilizing the knee joint capsule. Meniscal injuries are mainly caused by sports-related activities and age-related degeneration, which significantly reduce the life quality of patients. The injured meniscus has a very limited self-healing capability due to its poorly vascularized nature, specifically in the inner area. Surgical interventions are required to treat the injured meniscus, but the current treatments are not satisfactory because the post-surgery complications are frequently observed. Therefore, tissue regeneration approaches are developed to repair and functionally restore injured menisci. In this thesis, we …
Techniques To Improve Ultrasound-Switchable Fluorescence Imaging, Jayanth Kandukuri
Techniques To Improve Ultrasound-Switchable Fluorescence Imaging, Jayanth Kandukuri
Bioengineering Dissertations - Archive
Novel approaches to the improvement of ultrasound-switchable fluorescence (USF) imaging—a relatively new imaging modality that combines ultrasound and optical imaging techniques—have been proposed for early cancer detection. In USF, a high-intensity focused ultrasound (HIFU) beam is used to induce temperature rise within its acoustic focal region due to which a thermo-sensitive USF contrast agent undergoes a switch in its state by increasing the output of fluorescence photons. By using an increase in fluorescence, one can isolate and quantify the fluorescence properties within the ultrasonic focal area. Therefore, USF is able to provide fluorescence contrast while maintaining ultrasound resolution in tissue. …
Change Of Brain Network Connectivity Among Different Human Vigilance States Investigated By Eeg, Yudhajit Das
Change Of Brain Network Connectivity Among Different Human Vigilance States Investigated By Eeg, Yudhajit Das
Bioengineering Theses - Archive
Understanding the brain network connectivity associated with human vigilance states, to investigate the basic neural processes that underlie complex higher-order cognitive operations and functional domains, has become an important topic in biological science research. Also, there is growing concern about the prevalence and effects of sleep deprivation. Given the increasingly fast-paced nature of our society, the focus of the researchers is not only to identify the changes occurring within the brain during sleeping stages, but also to extract meaningful psycho-behavioral reason behind such change patterns which can lead to an improved understanding of brain activity during sleep which may further …
A Liver-Kidney Biosubsystem, Brian J. Budnik
A Liver-Kidney Biosubsystem, Brian J. Budnik
Bioengineering Dissertations - Archive
Each year many patients die waiting for a transplant organ to become available. Increasing the available number of transplant organs would save lives. The donor pool could be expanded if organs which are considered to be of uncertain viability could be utilized for transplantation. Currently, when there is any doubt about the viability of the organ, it is discarded. This occurs because there has been no way to confirm its viability other than to transplant it and risk the life of the recipient. Thus, many organs that are in fact good are wasted. Some might not be fully viable but …
Development Of Cancer Trap For Capturing Cancer Cells That Metastasize In The Peritoneal Cavity: A Preliminary Study, Chuka I. Okpokwasili
Development Of Cancer Trap For Capturing Cancer Cells That Metastasize In The Peritoneal Cavity: A Preliminary Study, Chuka I. Okpokwasili
Bioengineering Theses - Archive
Our laboratory has recently developed an implantable device - cancer trap that can recruit and trap circulating cancer cells inside the bloodstream. Since several types of cancer cells can metastasize inside the peritoneal cavity, this study explores the possibility of using cancer traps to recruit esophageal and prostate cancer cells inside the peritoneum. Using prostate cancer and esophageal cancer cells as model cancers, this thesis shows that cancer traps can capture cancer cells inside the peritoneal space. EPO releasing traps was also shown to reduce the spread of prostate cancer as well as esophageal cancer compared to controls. Using multiple …
T-Cell Membrane Camouflaged Nanoparticles For Treatment Of Melanoma, Gizem Oter
T-Cell Membrane Camouflaged Nanoparticles For Treatment Of Melanoma, Gizem Oter
Bioengineering Theses - Archive
Melanoma is one of the most aggressive skin cancers. The American Cancer Society reports that every hour, one person dies from melanoma. While there are a number of treatments currently available for melanoma (e.g. surgery, chemotherapy, immunotherapy, radiation therapy), these therapies face several problems, including inadequate response rates, high toxicity, and severe side effects due to non-specific delivery of anti-cancer drugs. To improve therapeutic efficiency and reduce these limitations, a multifunctional nanoparticle has been developed. Specifically, poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were coated with a cellular membrane derived from the T cell hybridoma, 19LF6 endowed with a melanoma-specific anti-gp100/ …
Unravelling The Mysteries Of Cognitive Decline In Cancer Survivors Using Functional Near Infrared Spectroscopy, Dhivya Srinivasan
Unravelling The Mysteries Of Cognitive Decline In Cancer Survivors Using Functional Near Infrared Spectroscopy, Dhivya Srinivasan
Bioengineering Theses - Archive
In recent years, there has been an increased incidence of cognitive impairment in cancer survivors as a side-effect of chemotherapy. This troubling deterioration in cognitive ability is commonly termed as 'Chemo brain' in which patients have problems with working memory, executive function, decision-making, concentration, visuospatial skills and attention which can pose problems to get on with day-to-day life. Breast Cancer is primarily adopted as the model for studying effects of 'chemobrain'. A better understanding of neural correlates & hemodynamic changes related to cognitive deficits would help clinicians to design targeted treatments and cognitive rehabilitation interventions. Aim: The primary goal of …
Design And Development Of Real-Time Single And Dual-Wavelength Dcs Systems To Study Skeletal Muscle Blood Perfusion And Oxygen Metabolism, Chandan Ganesh Bangalore Yogananda
Design And Development Of Real-Time Single And Dual-Wavelength Dcs Systems To Study Skeletal Muscle Blood Perfusion And Oxygen Metabolism, Chandan Ganesh Bangalore Yogananda
Bioengineering Theses - Archive
The aim of this study was to utilize Diffused Correlation Spectroscopy (DCS) to design and develop real-time single and dual wavelength systems and assess the physiological impacts of exercises on blood perfusion. The study consisted of 4 parts. Part 1 was to Re-design and develop a real-time single wavelength DCS system, part 2 was to validate this system by a cross validation method with the help of a concurrent study alongside Doppler ultrasound. Part 3 was to design & develop a real-time dual wavelength DCS system and part 4 was to validate the dual wavelength DCS system. DCS is a …
Light Reflectance Spectroscopy For The Detection Of Positive Surgical Margin Of Prostate Cancer, Henry Thek Chan
Light Reflectance Spectroscopy For The Detection Of Positive Surgical Margin Of Prostate Cancer, Henry Thek Chan
Bioengineering Theses - Archive
During a radical prostatectomy in the operating room, there exists a certain degree of risks of a surgical positive margin of prostate cancer. Positive surgical margins are defined as cancer that has spread beyond the margin of the prostate and into the surrounding tissues. Surgeons will be able to utilized the concept of Light Reflectance Spectroscopy (LRS) in real-time during surgery to help detect these positive surgical margins and to provide the surgeon a quantitative decision to remove the excess surrounding tissues. This modality would essentially lower the risks of positive surgical margins and prevent the recurrence of cancer, effectively …
The Mechanisms And Biomedical Applications Of An Nir Bodipy-Based Switchable Fluorescent Probe, Bingbing Cheng, Venugopal Bandi, Shuai Yu, Francis D’Souza, Kytai T. Nguyen, Yi Hong, Liping Tang, Baohong Yuan, Sundaresan Gobalakrishnan, William Chi-Shing Cho, Jamal Zweit
The Mechanisms And Biomedical Applications Of An Nir Bodipy-Based Switchable Fluorescent Probe, Bingbing Cheng, Venugopal Bandi, Shuai Yu, Francis D’Souza, Kytai T. Nguyen, Yi Hong, Liping Tang, Baohong Yuan, Sundaresan Gobalakrishnan, William Chi-Shing Cho, Jamal Zweit
Bioengineering Faculty Publications - Archive
Highly environment-sensitive fluorophores have been desired for many biomedical applications. Because of the noninvasive operation, high sensitivity, and high specificity to the microenvironment change, they can be used as excellent probes for fluorescence sensing/imaging, cell tracking/imaging, molecular imaging for cancer, and so on (i.e., polarity, viscosity, temperature, or pH measurement). In this work, investigations of the switching mechanism of a recently reported near-infrared environment-sensitive fluorophore, ADP(CA)2, were conducted. Besides, multiple potential biomedical applications of this switchable fluorescent probe have been demonstrated, including wash-free live-cell fluorescence imaging, in vivo tissue fluorescence imaging, temperature sensing, and ultrasound-switchable fluorescence (USF) imaging. The fluorescence …
A Dual-Modality System For Both Multi-Color Ultrasound-Switchable Fluorescence And Ultrasound Imaging, Jayanth Kandukuri, Shuai Yu, Bingbing Cheng, Venugopal Bandi, Francis D’Souza, Kytai Truong Nguyen, Yi Hong, Baohong Yuan, Jamal Zweit
A Dual-Modality System For Both Multi-Color Ultrasound-Switchable Fluorescence And Ultrasound Imaging, Jayanth Kandukuri, Shuai Yu, Bingbing Cheng, Venugopal Bandi, Francis D’Souza, Kytai Truong Nguyen, Yi Hong, Baohong Yuan, Jamal Zweit
Bioengineering Faculty Publications - Archive
Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently developed ultrasound-switchable fluorescence (USF) imaging technology with the conventional ultrasound (US) B-mode imaging. This dual-modality system can simultaneously image tissue acoustic structure information and multi-color fluorophores in centimeter-deep tissue with comparable spatial resolutions. To conduct USF imaging on the same plane (i.e., x-z plane) as US imaging, we adopted two 90 -crossed ultrasound …
Lung Protection By Inhalation Of Exogenous Solubilized Extracellular Matrix, Jinglei Wu, Priya Ravikumar, Kytai Truong Nguyen, Connie C.W. Hsia, Yi Hong
Lung Protection By Inhalation Of Exogenous Solubilized Extracellular Matrix, Jinglei Wu, Priya Ravikumar, Kytai Truong Nguyen, Connie C.W. Hsia, Yi Hong
Bioengineering Faculty Publications - Archive
Decellularized extracellular matrix (ECM) contains complex tissue-specific components that work in concert to promote tissue repair and constructive remodeling and has been used experimentally and clinically to accelerate epithelial wound repair, leading us to hypothesize that lung-derived ECM could mitigate acute lung injury. To explore the therapeutic potential of ECM for noninvasive delivery to the lung, we decellularized and solubilized porcine lung ECM, then characterized the composition, concentration, particle size and stability of the preparation. The ECM preparation at 3.2 mg/mL with average particle size <3 μm was tested in vitro on human A549 lung epithelial cells exposed to 95% O2 for 24 hours, and in vivo by tracheal instillation or nebulization into the lungs of rats exposed intermittently or continuously to 90% O2 for a cumulative 72 hours. Our results showed that the preparation was enriched in collagen, reduced in glycosaminoglycans, and contained various bioactive molecules. Particle size was concentration-dependent. Compared to the respective controls treated with cell culture medium in vitro or saline in vivo, ECM inhalation normalized cell survival and alveolar morphology, and reduced hyperoxia-induced apoptosis and oxidative damage. This proof-of-concept study established the methodology, feasibility and therapeutic potential of exogenous solubilized ECM for pulmonary cytoprotection, possibly as an adjunct or potentiator of conventional therapy.
Multi-Modality Magnetic Resonance Imaging In Typical And Atypical Developmental Human Brain, Minhui Ouyang
Multi-Modality Magnetic Resonance Imaging In Typical And Atypical Developmental Human Brain, Minhui Ouyang
Bioengineering Dissertations - Archive
Human brain development is structurally and functionally a nonlinear process and even with the tremendous advancements in the field of neuroimaging based on Magnetic Resonance Imaging (MRI) several significant questions remain un-answered. Although brain change and adaption are part of a lifelong process, the earliest phases of maturation, especially from birth to early childhood, are perhaps the most dramatic and important. During this early developmental period, the structural and functional organization of the brain are continuously shaped by a combination of synaptogenesis, dendritic arborization, myelination and synaptic pruning. The current research aims to use more recently developed MRI techniques of …
Automated Voxel Classification Used With Atlas-Guided Diffuse Optical Tomography For Assessment Of Functional Brain Networks In Young And Older Adults, Lin Li, Mary Cazzell, Olajide Babawale, Hanli Liu
Automated Voxel Classification Used With Atlas-Guided Diffuse Optical Tomography For Assessment Of Functional Brain Networks In Young And Older Adults, Lin Li, Mary Cazzell, Olajide Babawale, Hanli Liu
Bioengineering Faculty Publications - Archive
No abstract provided.