Engineering Commons^™

Pathogenic Dna Detection Using Dna Hairpins: A Non-Linear Hybridization Chain Reaction Platform, Lance Novak, Tamara L. Kinzer-Ursem

The Summer Undergraduate Research Fellowship (SURF) Symposium

Currently, 3.2 billion people are at risk of being infected with malaria, with 1.2 billion of those being at high risk (>1 in 1000 chance of getting malaria in a year). Thus, there is a need for a biosensor that is highly sensitive, cost effective, and simple to use for point-of-care diagnosis. The biosensing platform, PathVis, has achieved this by measuring changes in fluid properties after a loop-mediated isothermal amplification (LAMP). LAMP is a DNA amplification system that requires enzymes and a temperature of 65degrees C. LAMP currently limits PathVis by being costly, requiring refrigeration, and difficult to design. …

Verification Of Tfit Code Numerical Method For Flow Excursion Simulation, Patrick S. Foster, Subash Sharma, Martin L. Bertodano

The Summer Undergraduate Research Fellowship (SURF) Symposium

This research is aimed towards accurately modeling and predicting the onset of the two-phase flow excursion instability using the code TFIT (Two Fluid Interfacial Temperature). In order to do this we first had to show that the numerical diffusion of the code’s finite difference equations could be reduced to an insignificant level by decreasing the mesh size.

Understanding and being able to accurately model flow excursion can help us understand how to prevent the potential negative effects of this instability. We are using a two-fluid model with physics-based closure relations. The results will be validated against the experimental data available …

Capsule-Based Dropwise Additive Manufacturing With Pharmaceutical Suspensions, Kaitlyn N. Buck, Andrew J. Radcliffe, Gintaras V. Reklaitis

The Summer Undergraduate Research Fellowship (SURF) Symposium

Current manufacturing of pharmaceutical products focuses on creating a standard dosage of the active pharmaceutical ingredient (API); however, dosages often need to be altered or customized to account for a patient’s age, weight, comorbidity, and other genetic factors. A potential method for dispensing precise dosages of API suspensions through dropwise addition is detailed in the following paper. By using a drop-on-demand printing rig, a series of suspensions comprised of varying volume fractions of a micron-scale API in a carrier fluid were printed, and individual drop volumes were analyzed using high-resolution imaging. From this, capsules with 1 mg dosages and 100 …

Examination Of Ordering Effects In Sound Evaluations, Yiyun Zhang, Patricia Davies, Daniel Carr

The Summer Undergraduate Research Fellowship (SURF) Symposium

It is difficult to predict sound annoyance responses because annoyance is an inherently subjective concept and the evaluation standard could be different from each person. Usually, annoyance models are functions of strengths of sound characteristics only, and these models can help engineers to improve sounds that machines make and minimize annoyance levels, but other factors can also influence annoyance ratings of products. The main goal of this research was to investigate ordering effects in sound evaluations when people are hearing a set of sounds in sequence. Of particular interest is how the response to a sound influences the response to …

A Chronically Implanted, Continuous Ph Monitoring System For Rats, Ryan B. Budde, Pedro P. Irazoqui

The Summer Undergraduate Research Fellowship (SURF) Symposium

Many body systems operate within a strict pH range, and any deviation can cause harm. pH measurement systems are used in many biomedical research fields. Measurement systems have been able to continuously record pH for a short period of time wirelessly, or over a long period of time with wires, but no system is currently capable of long term, wireless, continuous pH recording. This paper proposes a new pH measurement system that is capable of such measurement. The system is composed of inexpensive, micro-scale, and easy to manufacture pH sensitive and reference electrodes and a data acquisition and transmission module …

Buckling Of Cylindrical Shells Under Wind Loading, Tianlong Sun, Eyas Azzuni, Sukru Guzey

The Summer Undergraduate Research Fellowship (SURF) Symposium

Cylindrical shells such as aboveground steel storage tanks are widely used to store gases, chemicals and fuels. Stiffener rings are introduced to prevent cylindrical shells from buckling due to wind loads. The current top wind stiffener design rules used in the USA for such cylindrical storage tanks are specified in API 650. However, the design methodology of top stiffener rings is overly conservative. Therefore, relaxation of the design rules for the top stiffener is investigated in this study. The API 650 mandated top stiffener size was reduced to the same size as the mandated intermediate stiffener ring in this study. …

Pursuit Evasion With Multiple Pursuers : Capturing A Ground Vehicle On A Road Network With Multiple Drones, Blake Wilson, Shreyas Sundaram, Amritha Prasad

The Summer Undergraduate Research Fellowship (SURF) Symposium

Unmanned Aerial Vehicles (UAV) have many military and civilian applications, one of which is monitoring a given area (such as a road network) for threats. An important question in this application is to determine the latest time to dispatch UAVs for the guaranteed capture of threats attempting to travel through the network. In this work, we consider a pursuit evasion scenario with multiple pursuers (UAVs) trying to catch a single evader (ground vehicle), where information about the evader’s path is provided by ground sensors. In this scenario, we consider the problem of finding the maximum delay with which the pursuers …

Post-Earthquake Fire Assessment Of Steel Buildings, Yi Li, Rachel Chicchi, Amit H. Varma

The Summer Undergraduate Research Fellowship (SURF) Symposium

Resilience of structural systems after hazardous events is a crucial concern of building design. An abundance of research has focused on hazards such as seismic and fire separately. This project conducted a multi-hazard study on steel buildings considering both seismic and fire damage. A literature review of the behavior of steel-framed buildings due to fires after earthquakes, known as post-earthquake fires (PEF), is offered. The new PEF methodology, delivered in this study, starts with creating a three dimensional (3D) model of the examined steel building using the finite element method software, ABAQUS. Next, varying intensities of seismic and fire hazards …

Analysis Of The Flow Behaviors Of Corn Meal During Extrusion, Daniel N. Hauersperger, Martin R. Okos, Troy Tonner

The Summer Undergraduate Research Fellowship (SURF) Symposium

Food extrusion can be used to make many products we consume today, including pasta, cereals and more. The ability to predict the characteristics of the final product from an extruder using raw material characteristics and operating conditions is vital to the extrusion process. In order to answer this need, the flow behavior of corn meal was measured in a lab viscometer (off-line) and compared to the flow behaviors from an extruder (in-line) at three different moisture contents (32.5%, 35%, 37.5% wet basis). The extruder and product are heated through the friction of the corn meal passing through the barrel not …

Structure-Force Field Generator For Molecular Dynamics Simulations, Carlos M. Patiño, Lorena Alzate, Alejandro Strachan

The Summer Undergraduate Research Fellowship (SURF) Symposium

Atomistic and molecular simulations have become an important research field due to the progress made in computer performance and the necessity of new and improved materials. Despite this, first principle simulations of large molecules are still not possible because the high computational time and resources required. Other methods, such as molecular dynamics, allow the simplification of calculations by defining energy terms to describe multiple atom interactions without compromising accuracy significantly. A group of these energy terms is called a force field, and each force field has its own descriptions and parameters. The objective of this project was to develop a …

Modeling The Aqueous-Phase Copper Ion-Exchange Behavior Onto Ssz-13 Zeolites, Brian N. Bayer, Rajamani Gounder, Trevor M. Lardinois

The Summer Undergraduate Research Fellowship (SURF) Symposium

Copper-exchanged zeolites are utilized as catalysts for the selective catalytic reduction of nitrogen oxides, which are atmospheric pollutants found in diesel engine exhaust. The total amount of copper ions and the types of copper species (Cu(II) or Cu(II)OH) exchanged onto a zeolite can be varied. Copper is exchanged onto SSZ-13 (an aluminosilicate zeolite with the chabazite topology) during a process known as aqueous ion exchange, where the zeolite is mixed in a copper-containing solution. The distribution of copper on SSZ-13 is influenced by exchange conditions, including the molarity, temperature, and pH of the copper solution. The effect of exchange conditions …

Cylindrical Shell Based Phase Transforming Cellular Materials: Designing A Recoverable Energy Dissipating Material, Gordon F. Jarrold, David Restrepo, Nilesh Mankame, Pablo Zavattieri

The Summer Undergraduate Research Fellowship (SURF) Symposium

Energy dissipating materials are used in a variety of impact events to protect more important parts of a system; one example of this is a football player’s helmet protecting a brain. A major drawback to classic energy dissipating materials however is that they dissipate energy through plastic deformation, meaning that after a single use, permanent deformations will prevent the material from being reusable to the same capacity as initially. We have designed a 1D cellular material in which geometric phase transformations in cylindrical shell elements are the primary energy dissipating mechanism, allowing for recoverability after use while keeping high energy …

Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama

The Summer Undergraduate Research Fellowship (SURF) Symposium

Parkinson’s Disease (PD) is a common neurodegenerative disease with over 200,000 new cases each year. In general, the cause of the disease is unknown, but oxidative stress inside of neurons has been associated with the disease’s pathology for some time. Currently, techniques to study the onset of PD inside of neurons are limited. This makes treatments and causes difficult to discover. One solution to this has been fluorescent protein biosensors. In short, these proteins can be engineered to glow when a certain state is achieved inside a cell. The present research discusses the engineering of a genetically-encoded fluorescent protein (FP) …

Virtual-Source Based Accurate Model For Predicting Noise Behavior At High Frequencies In Nanoscale Pmos Soi Transistors, Vaibhav R. Ramachandran, Saeed Mohammadi, Sutton Hathorn

The Summer Undergraduate Research Fellowship (SURF) Symposium

Complementary Metal Oxide Semiconductor (CMOS) technology at the nanometre scale is an excellent platform to implement monolithically integratedsystems because of the low cost of manufacturing and ease of integration. Newly developed CMOS Silicon on Insulator (SOI) transistors that are currentlydeveloped are suitable for use in radio frequency circuits. They find applications in many areas such as 5G telecommunication systems, high speed Wi-Fi andairport body-scanners. Unfortunately, the models for CMOS SOI transistors that are currently used in these circuits are inaccurate because of their complexity.The models currently used require the optimization of more than 200 variables. This paper aims to accurately …

Effect Of Particle Interactions On Powder Flow Behavior, Ivana A. Penagos, Carolina P. Mora, Teresa Carvajal

The Summer Undergraduate Research Fellowship (SURF) Symposium

The study of powder flow behavior is essential for the development of processing technologies in many industries. In fact, powders have a major function in diverse types of manufacturing, such as pharmaceuticals, foods, chemicals, materials, minerals and cosmetics. This leads to an increasing demand for the development of reliable methods to assess powder flow problems in industry. This research intends to provide a general insight into how surface interactions and particle properties may alter powder flowability. The materials used for this study were lactose, starch, milk powder, cocoa and chocolate. These vary in their interparticle forces as well as on …

Finite Element Analysis And Biological Growth Realization Using Robot Swarms, Cody W. Dorbin, Adrian Buganza-Tepole

The Summer Undergraduate Research Fellowship (SURF) Symposium

Our understanding of growth and remodeling of biological systems has increased in the past two decades; however, this knowledge has not yet been used in human-designed systems or engineering applications. This project studies designing and building a network of robots that mimics the biological behavior of growth driven by cell-cell communication and control networks. The objective of this research is to harness the principles that govern tissue adaptation and morphogenesis, where peer-to-peer local communication determines global properties, to create human-made engineering systems with life-like capabilities. We used Arduino microcontrollers to control an individual robot in an expandable 3d-printed cuboid shell. …

Enhancing The Performance Of A Transportable Environmental Control Unit (Ecu) Operated In High-Temperature Climates, Quan Jun Sun, Ammar M. Bahman, Eckhard A. Groll

The Summer Undergraduate Research Fellowship (SURF) Symposium

Numerous people live or venture into environments, such as the Middle East, where the temperatures can skyrocket as high as 54°C. This leaves many air conditioners unable to operate efficiently. Although much research has been conducted for incorporating vapor injection processes into refrigeration systems, such as ones used in supermarkets, little has been researched regarding the application in extreme heat environments. While most basic air conditioning units do not require the addition of a vapor injection process along with an economizer, this project requires the air conditioning (AC) unit to operate under extreme high temperature conditions. The project investigates the …

Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang

The Summer Undergraduate Research Fellowship (SURF) Symposium

Fluorescence microscopy, which allows multiple-color imaging, plays an important role in observing structures inside cells with high specificity. The advent of super-resolution fluorescence microscopy, or nanoscopy techniques such as single-molecule switching nanoscopy (SMSN), has extended the application range of fluorescence microscopy beyond the diffraction limit, achieving up to 10-fold improvement in spatial resolution. At the same time, the recent development of expansion microscopy (ExM) allows samples to be physically expanded by 4-fold in the lateral dimensions providing another independent method to resolve structures beyond the diffraction limit. When combined, ExM-SMSN makes it possible to achieve another significant leap in resolution …

Bacterial Motility And Its Role In Biofilm Formation, Clayton J. Culp, Arezoo M. Ardekani, Adib Ahmadzadegan

The Summer Undergraduate Research Fellowship (SURF) Symposium

Bacterial biofilms are known to cause millions of dollars in damage in the medical industry per year via infection of central venous catheters, urinary catheters, and mechanical heart valves. Unfortunately, there are some characteristics of biofilm formation that are yet to be fully understood. Recently much work has been done to investigate the motility characteristics of bacteria with hopes of better understanding the phenomena of biofilm formation. Still, one of the least understood stages is bacterial attachment or adhesion, a process designed to anchor bacteria in an advantageous environment. Providing a better understanding of bacterial motility near solid interfaces will …

Metabolic Comparison Of Wild-Type And Transgenic Synechocystis Pcc 6803 Cyanobacteria, Ian A. Mcluckey, John A. Morgan, Joel Yu King Hing

The Summer Undergraduate Research Fellowship (SURF) Symposium

The Calvin-Benson (CBB) cycle is an essential part of nature. This phenomenon allows carbon molecules in carbon dioxide from the atmosphere to be converted into useful energy in the form of sugars. Cyanobacteria are single-celled organisms capable of utilizing energy from sunlight to drive this cycle and are also readily engineered. In hopes of improving this cycle, we compared a wild-type version of the Synechocystis PCC6803 cyanobacteria to an engineered version overexpressing the enzyme FBA (fructose-biphosphate aldolase), called 70 glpX, to deduce how the overexpressing strain is able to be more photosynthetically efficient. To do this, comparative metabolomics were done …

3d-Printed Microswimmers With Nanostructures For Color Tracking, Cara Koepele, Maria Guix, David Cappelleri

The Summer Undergraduate Research Fellowship (SURF) Symposium

Two-Photon Polymerization (TPP) is a fabrication technique based on the localized linking of photosensitive materials resulting from femtosecond – a quadrillionth of a second – exposure to a laser. Such materials are based on building blocks named monomers that combine under certain stimuli (i.e. light) to form chains or complex networks. Utilization of TPP as a method for micro-3D printing has expanded the field of microrobotics, which presents medical solutions for minimizing procedure invasiveness as well as increasing treatment and diagnosis accuracy. One of the challenges in achieving desired accuracies is designing trackable features onto a microrobot. With the capabilities …

Localized Blood Occlusion Generation In An In-Vitro Circulatory Catheter System, Ryan D. Harris, Qi Yang, Hyowon Lee

The Summer Undergraduate Research Fellowship (SURF) Symposium

Hydrocephalus is a debilitating neurological disorder that involves the accumulation of cerebrospinal fluid in a ventricle of the brain. The implantation of a catheter commonly treats hydrocephalus with drainage. These catheters have a short lifespan due to obstruction from biological materials. Shunt systems have an extremely high failure rate of more than 40% failed within 1 year and up to 85% failed within 10 years. Previously, polymer-based flexible implantable magnetic micro-actuators were developed to clean up the catheter by mechanical vibration. We have demonstrated clearing of bacteria attachment and are proceeding to examine clearing effects on larger clotting materials, such …

Identifying The Pathways And Experiences Of Asian Students' Engineering Major Choice, Christine Chern, Xinrui Xu, Joyce Main

The Summer Undergraduate Research Fellowship (SURF) Symposium

Asian students’ pathways and experiences in engineering are not well-studied. Understanding pathways and experiences can inform students, parents, educators, and policymakers how to better approach the engineering major choice process. This study identifies pathways and experiences of Asian students choosing to major in engineering. Eleven semi-structured interviews of Asian students at a major research institution were analyzed in NVivo using thematic analysis. In this study, 8 Asian students chose engineering as a field of study and subsequently chose an engineering major while 3 chose an engineering major directly. Students following either pathway shared catalyzing interests, such as math and science, …

Atomistic Simulations Of Novel Nanoscale Semiconductor Devices: Resistance Switches And Two-Dimensional Transistors, Joseph P. Anderson, Mahbubul Islam, David Guzman, Alejandro Strachan

The Summer Undergraduate Research Fellowship (SURF) Symposium

As transistors get smaller, we are achieving record levels of memory density. However, there is a limit to how small transistors can be made before their functionality breaks down. Thus alternatives to traditional transistor technology are needed. The two such technologies we examined are: resistance switching devices, which reversibly grow metal filaments through a dielectric, and two-dimensional transistors, which are capable of breaking through the scalability limit of traditional transistors. In order to design resistance switching devices which create filaments with some level of consistency, the dynamics of the filament formation need to be explored. Herein we model this process …

Modal Phonon Transport Across Interfaces By Non-Equilibrium Molecular Dynamics Simulation, Yang Zhong, Tianli Feng, Xiulin Ruan

The Summer Undergraduate Research Fellowship (SURF) Symposium

Phonons represent the quantization of lattice vibration, responsible for heat transfer in semiconductors and dielectrics. Phonon heat conduction across interfaces is crucially important for the thermal management of real-life devices such as smartphones, electric vehicles, and satellites. Although recent studies have broadly investigated spectral phonon contribution to lattice thermal conductivity, the mechanism of phonon modal transport across interfaces is still not well-understood. Previous models, including the acoustic mismatch model (AMM) and diffuse mismatch model (DMM), only consider elastic process while neglecting inelastic phonon contributions. Herein, we employ spectral Non-Equilibrium Molecular Dynamics Simulation (NEMD) to probe the temperature and heat flux …

Single Cell Epigenetics, Li F. Lin, Chongli Yuan, Oscar F. Sanchez-Medina

The Summer Undergraduate Research Fellowship (SURF) Symposium

Epigenetics refers to the chromatin modifications that can change the expression of phenotypes but does not alter the underlining DNA. These modifications include but are not limited to histone methylation, histone acetylation, and chromatin folding. Studies suggested that methylation can activate or silence the expression of genes; and that histone acetylation can alter the chromatin structure. The changes in chromatin structure can expose hidden transcriptional factor binding sides and thus affect the differentiation potential. Moreover, these histone modifications are affected by the introduction of different metabolites and cellular microenvironment. To achieve a better understanding of how metabolites and environmental chemical …

Study Of Persistent Slip Bands Formed By Low Cycle Fatigue On Nickel-Based Superalloys At Room Temperature, Anjola Uprety, Sae Matsunaga, Michael S. Titus Dr., Michael D. Sangid Dr., Alberto W. Mello Dr.

The Summer Undergraduate Research Fellowship (SURF) Symposium

The ability of Ni-based superalloys to withstand temperatures in excess of 1100⁰C makes them useful for applications in the hottest components in jet engines, gas turbines, and thrust engines. Increasing the efficiency of these gas turbine engines helps to reduce the fossil fuel consumption and the production of greenhouse gasses. A common mode of failure in these Ni-based superalloys is low cycle fatigue, in which narrow regions of high dislocation density, which are known as persistent slip bands (PSBs), can develop and lead to crack initiation. A detailed understanding of the formation and structure of PSBs has eluded …

A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem

The Summer Undergraduate Research Fellowship (SURF) Symposium

In excitatory neurons, the ability of a synaptic connection to strengthen or weaken is known as synaptic plasticity and is thought to be the cellular basis for learning and memory. Understanding the mechanism of synaptic plasticity is an important step towards understanding and developing treatment methods for learning and memory disorders. A key molecular process in synaptic plasticity for mammalian glutamatergic neurons is the exocytosis (delivery to the synapse) of AMPA-type glutamate receptors (AMPARs). While the protein signaling pathways responsible for exocytosis have long been investigated with experimental methods, it remains unreasonable to study the system in its full complexity …

The Effects Of Compaction On Reconstitution Characteristics Of Dairy Powder, Michelle P. Dixon, Rose Prabin Kingsly Ambrose, Dhananjay Ashok Pai

The Summer Undergraduate Research Fellowship (SURF) Symposium

Milk powders are becoming more ubiquitous within the food industry, and they offer consumers a convenient form for direct intake of dairy. The costs associated with dairy are reduced when products are converted to powder, which markedly increases shelf life at ambient temperatures. Creating compacts from powders further improves cost efficiency by reducing volume. However, milk powders typically possess poor rehydration properties, such as a slow dispersion rate and low solubility, and powder compaction additionally prolongs rehydration time, reducing consumer acceptability. Previous studies have shown that modifying dairy products before or during the drying process can improve rehydration properties, but …

Design A Continuously Tunable Microwave Filter With A Generalized Constant And Frequency Mapping Technique, Josh Shao, Runqi Zhang, Dimitrios Peroulis

The Summer Undergraduate Research Fellowship (SURF) Symposium

As the demand for agile and accurate communication increases, the fundamental of communication must be improved to support the needs. Current communication operates on an agreement, that each network company pays government to get licensed a range of frequency on the spectrum, and no others are legally allowed to use. This allows a limited number of users to operates at the same time. As the number of users increase, the spectrum become congested and user experience problem like dropping call. This problem can be solved, if users can be shifted between different licensed frequencies, depending on the capacity of the …