Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons™

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 5551 - 5580 of 6294

Full-Text Articles in Engineering

System For And Method Of Performing Evaluation Techniques On A Log Or Round Timber, Xiping Wang, Robert J. Ross, James A. Mattson, John Erickson, John W. Forsman, Earl A. Geske, Michael A. Wehr May 2006

System For And Method Of Performing Evaluation Techniques On A Log Or Round Timber, Xiping Wang, Robert J. Ross, James A. Mattson, John Erickson, John W. Forsman, Earl A. Geske, Michael A. Wehr

Michigan Tech Patents

A system for and method of evaluating a log. The system includes an analysis module having at least one input terminal connectable to the at least one input device. The at least one input terminal is operable to receive at least one signal representing at least one measured property of the log and at least one determined parameter of the log determined in response to an energy being applied to the log. The analysis module further includes a processor coupled to the at least one input terminal. The processor determines a predictive modulus of elasticity (MOE) of the log based …


2nd Annual Undergraduate Research Conference Abstract Book, University Of Missouri--Rolla Apr 2006

2nd Annual Undergraduate Research Conference Abstract Book, University Of Missouri--Rolla

Undergraduate Research Conference at Missouri S&T

No abstract provided.


2006 - The Eleventh Annual Symposium Of Student Scholars Apr 2006

2006 - The Eleventh Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Eleventh Annual Symposium of Student Scholars, held on April 7, 2006. Includes abstracts from the presentations and posters.


Santa Clara Magazine, Volume 47 Number 4, Spring 2006, Santa Clara University Apr 2006

Santa Clara Magazine, Volume 47 Number 4, Spring 2006, Santa Clara University

Santa Clara Magazine

8 - A DREAM OPPORTUNITY by Monte Lorenzet. Santa Clara University's sleep lab is one of just a handful of similar research facilities at undergraduate institutions. Students, alumni, faculty, and the research community are all benefiting from the fledgling lab.

10 - SCIENCE AND SOCIAL JUSTICE by Kim Kooyers. Social entrepreneurs use technology to address some of the world's urgent needs. Last November, some of these innovators were honored at the Tech Museum Awards, an international awards program.

14 - GIGANTES Y CABEZUDOS by Elizabeth Kelley Gillogly '93. In an intensive workshop featuring seasoned artists from Spain, SCU students explored …


Development Of Self-Assembled Monolayer-Based Cell Culture Platform Towards Fabrication Of A Three-Dimensional Bioreactor, Rajendra Kandoor Aithal Apr 2006

Development Of Self-Assembled Monolayer-Based Cell Culture Platform Towards Fabrication Of A Three-Dimensional Bioreactor, Rajendra Kandoor Aithal

Doctoral Dissertations

The extracellular matrix (ECM) plays an important role in regulating a number of cellular properties and functions like cell differentiation, cell synthesis and degradation, cell viability and proliferation, cell function, and cell aging. Surface modification of planar substrates with self-assembled monolayers (SAMs) is a promising technique to achieve stable ECMs.

In this work, substrates such as silicon (Si), gallium arsenide (GaAs) and indium tin oxide (ITO) substrates were modified with SAMS containing amino (-NH2), methyl (-CH3), thiol (-SH) and carboxylic (-COOH) end groups and characterized using contact angle measurements, surface infrared (IR) spectroscopy and atomic force microscopy (AFM). Different cell …


Automated Oxidase-Coupled Amperometric Microsensor With Integrated Electrochemical Actuation System For Continuous Sensing Of Saccharoids, Yadunandana Yellambalase, Jongwon Park, Chang-Soo Kim, Minsu Choi, Nohpill Park, Fabrizio Lombardi Apr 2006

Automated Oxidase-Coupled Amperometric Microsensor With Integrated Electrochemical Actuation System For Continuous Sensing Of Saccharoids, Yadunandana Yellambalase, Jongwon Park, Chang-Soo Kim, Minsu Choi, Nohpill Park, Fabrizio Lombardi

Electrical and Computer Engineering Faculty Research & Creative Works

Recent developments for biosensors have been mainly focused on miniaturization and exploratory use of new materials. It should be emphasized that the absence of a novel "in-situ self-calibration/diagnosis technique" that is not connected to an external apparatus is a key obstacle to the realization of a biosensor for continuous use with minimum attendance. To address this deficiency, a novel needle-type biosensor system with fully automated operations is being developed, in which a novel oxidase-coupled amperometric sensor with oxygen depleting/generating actuator is interfaced with an electrochemical instrument and a perfusion system. Labview virtual instrument has been also developed to oversee the …


Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • Limestone-Based Material for Arsenic Removal from Drinking Water, Chelsea Campbell and others, Dept of Chemistry, WKU
  • Opportunistic Water Education, Amanda Abnee Gumbert, Extension Associate for Environmental and Natural Resources Issues, UK
  • Ten-Year Solute Concentration Patterns in Two Streams of Contrasting Land-Use in Western Kentucky and Tennessee, Susan P. Hendricks, Hancock Biological Station, Murray State University
  • Pathogen TMDL Development using Load Duration Curves for Two Stream Segments in Rockcastle County, Kentucky, Joseph M. Ferguson, Kentucky DOW
  • Beargrass Creek Water Quality Tool and TMDLs, Ward Wilson and others, Tetra Tech
  • Pathogen and Sediment Transport in Muddy Creek …


Session 2d: Modeling, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 2d: Modeling, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • Simplification of Access to Hydrologic Data for Kentucky through an Online, Interactive GIS Tool, K. R. Odom and M. A. Ayers, USGS, Louisville
  • The Kentucky Watershed Modeling Information Portal’s User Needs Assessment, Data Matrix and Use Case, K. L. Schaffer, K. R. Odom, and others, FMSM and USGS
  • A Comparison of Manually and DEM Delineated Watersheds, Andrew Kellie, Jane Benson, and Mike Kemp, Dept of Industrial and Engineering Technology, Murray State University
  • Of Farm Ponds and Sinkholes: Automated Feature Extraction from Kentucky’s NAIP Imagery, Demetrio Zourarakis, Kentucky Division of Geographic Information


Session 2c: Planning, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 2c: Planning, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • US EPA’s New Guidance on Watershed-Based Plans for Restoration and Protection, Barry Tonning, Tetra Tech, Mount Sterling, KY
  • Louisville Water Company – Wellhead Protection Plan, Marsha L. Taylor Meyer, Louisville Water Company
  • Lessons Learned Reforesting the Bluegrass, H. David Gabbard, LFUCG, Lexington, KY
  • Low-Flow Characteristics of Streams in Kentucky, G.R. Martin, D.W. Evans, and K.R. Odom, USGS, Louisville


Session 2b: Sediments, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 2b: Sediments, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • The New Contractor EPSC Certification Program Developed by the City of Bowling Green Kentucky: Educating and Involving Contractors in NPDES Phase II Compliance, Jeff Lashlee, City of Bowling Green, Beth Chesson, CEC, Inc., and April Barker, AMEC Earth and Environmental, Nashville, TN
  • Kentucky Best Management Practices for Controlling Erosion, Sediment, and Pollutant Runoff from Construction Sites: Planning and Technical Specifications Manual, Richard Walker and Barry Tonning, Tetra Tech, Lexington
  • Sediment Monitoring Efforts in the Upper Green River Basin in Support of the Kentucky Conservation Reserve Enhancement Program, S.T. Kenworthy, Dept of Geography and Geology, WKU
  • Land-Use Fingerprinting …


Session 2a: Nonpoint Source, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 2a: Nonpoint Source, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • Little River Watershed Delineation and “Misbehaved” Karst Drainage, J.A. Ray and R.J. Blair, Kentucky Division of Water
  • Comprehensive Commonwealth Water Education Project, David Howarth, Keith Mountain, and Kristen Dunaway, Dept of Geography and Geosciences, U of L
  • Assessment of Water Quality Trends in the Upper Cumberland River Basin: Focus on Pathogen Impairment, Lindell Ormsbee and Ramesh Teegavarapu, KWRRI, UK
  • Watershed & Land Use Planning: A BMP Technology Transfer Project (A Case Study of the Dry Run Watershed Basin), Rachel Phillips, Brad Frazier, and Sandy Camargo, Georgetown/Scott County Planning Commission


Session 1d: Monitoring, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 1d: Monitoring, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • An Examination of Statewide Watershed Watch Project Monitoring Data for 2005 Using GIS, Ken Cooke, Kentucky Division of Water, Frankfort
  • Taking Watershed Watch to the Next Level: Grabbing the Attention of Local Elected Officials, Ken Cooke, Kentucky Division of Water, H. David Gabbard, LFUCG, Lexington
  • Water Quality Data from Citizen Monitoring in the Licking River Region Kentucky 1998-2005: Trends and Issues, M.F. Hult, Daniel Carter Beard Environmental Center, and B.C. Reeder, Center for Environmental Education, Morehead State University
  • Water Quality in the Upper Licking River Basin 2003-2005, Brian Reeder and others, Dept of Biological and Environmental …


Session 1c: Superfund, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 1c: Superfund, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • The University of Kentucky Superfund Basic Research Program: Overview and Examples of Research Projects, Bernhard Hennig, Leonidas Bachas, and Lindell Ormsbee, UK
  • Reductive Dechlorination of Toxic Organics by Bimetallic Nanoparticles in Polyacrylic Acid (PAA) Functionalized MF Membranes, Jian Xu and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
  • Chlorinated Organic Compounds Destruction by Modified Fenton Reaction Involving Immobilized Iron-Chelate, YongChao Li, Leonidas Bachas, and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
  • An Outreach Program Translates Basic Research for Superfund Communities to Improve Health through Nutrition, Lisa Gaetke and Sandra Bastin, Dept Nutrition and Food Science, …


Session 1b: Groundwater, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 1b: Groundwater, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • A Brief History and Current State of Groundwater Protection in Kentucky, James Webb, Beverly Oliver, and Peter Goodmann, Kentucky DOW, Groundwater Branch
  • Summary of Groundwater Quality Data in the Jackson Purchase Region, Kentucky, E.G. Beck, J.S. Dinger, and P.C. Inkenbrandt, KGS
  • Groundwater-Quality Assessment and Shallow Aquifer Model of Calloway County, Kentucky, P.C. Inkenbrandt, E.G. Beck, and J.S. Dinger, KGS
  • Suspended Sediment and Pathogen Transport in Two Inner Bluegrass Karst Ground-Water Basins, A.E. Fryar and others, Dept Earth and Environmental Sciences, UK
  • Locating and Mapping Domestic Water Wells in Marshall County, Kentucky, Wendy D. Langhi, Western …


Session 1a: Water Quality, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Session 1a: Water Quality, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

  • Use of Sunfish and Stoneroller Minnows as Sentinel Monitors of PCB Contamination in Freshwater Streams in Kentucky, D.J. Price and W.J. Birge, Dept of Biology, UK
  • Inferring Causes of Biological Impairment in Appalachian Streams: Watershed-Based Problem Formulation and Integration of Multiple Lines of Evidence, Jon Ludwig and others, Tetra Tech
  • Relative Importance of Water and Dietary Cadmium: Toxicity to Ceriodaphnia Dubia, Agus Sofyan and W.J. Birge, Dept of Biology, UK
  • The Graywater Story at Curtis Pike, Jack Kieffer and others, Appalachia - Science in the Public Interest, Mt. Vernon


Proceedings Of 2006 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky Mar 2006

Proceedings Of 2006 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was funded in part by the U.S. Environmental Protection Agency, with Clean Water Act, Section 319(h) grant money through the Kentucky Division of Water and the Kentucky Waterways Alliance, #C9994861-00.

Planning for this conference was conducted as part of the state water resources research annual program with the support and collaboration of the Department of Interior, U.S. Geological Survey and the University of Kentucky Research Foundation, under Grant Agreement No. 01HQGR0133.

The views and conclusions contained in this document are those of the abstract authors and should not be interpreted as necessarily representing the official policies, either expressed …


Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr. Mar 2006

Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr.

Theses and Dissertations

Chlorinated hydrocarbons and their degradation products are among of the most common organic groundwater contaminates in the United States. These compounds attack the central nervous system in animals and can affect the photosynthesis of plants. These compounds are also resistant to degradation in the environment and, because of this, pose a risk to any ecosystem in which they are present. This study identified the dominant microbial species in a constructed treatment wetland at Wright-Patterson AFB, Dayton, Ohio using 16S rRNA gene sequence analysis. Samples were taken from three different depths and during each of the four seasons. These samples were …


In Vitro Toxicity Of Aluminum Nanoparticles In Rat Alveolar Macrophages, Andrew J. Wagner Mar 2006

In Vitro Toxicity Of Aluminum Nanoparticles In Rat Alveolar Macrophages, Andrew J. Wagner

Theses and Dissertations

Nanomaterials, which are by definition in the 1 - 100 nanometer range, have numerous possible benefits to society, but currently there is a lack of data that characterizes these materials effects on human health and environment. In general nanomaterials are of interest to the Air Force because of their applications in electronics, sensors, munitions and energetic/reactive systems. Nanoparticles such as aluminum have been considered for enhancing propulsion in solid rocket fuel. To date, only a few studies have looked at the toxicological effects of direct exposure to nanoparticles, none with aluminum. It is important to increase the understanding of the …


Adaptive Evolution Of Chloroplast Genome Structure Inferred Using A Parametric Bootstrap Approach, Liying Cui, Jim Leebens-Mack, Li-San Wang, Jijun Tang, Linda Rymarquis, David B. Stern, Claude W. Depamphilis Feb 2006

Adaptive Evolution Of Chloroplast Genome Structure Inferred Using A Parametric Bootstrap Approach, Liying Cui, Jim Leebens-Mack, Li-San Wang, Jijun Tang, Linda Rymarquis, David B. Stern, Claude W. Depamphilis

Faculty Publications

Background
Genome rearrangements influence gene order and configuration of gene clusters in all genomes. Most land plant chloroplast DNAs (cpDNAs) share a highly conserved gene content and with notable exceptions, a largely co-linear gene order. Conserved gene orders may reflect a slow intrinsic rate of neutral chromosomal rearrangements, or selective constraint. It is unknown to what extent observed changes in gene order are random or adaptive. We investigate the influence of natural selection on gene order in association with increased rate of chromosomal rearrangement. We use a novel parametric bootstrap approach to test if directional selection is responsible for the …


Simulated Performances Of A Heat Pump System For Energy And Water Conservation In Open And Confined Greenhouse Systems, Ilhami Yildiz, D. P. Stombaugh Jan 2006

Simulated Performances Of A Heat Pump System For Energy And Water Conservation In Open And Confined Greenhouse Systems, Ilhami Yildiz, D. P. Stombaugh

BioResource and Agricultural Engineering

The purpose of this study was to determine and compare the performances of conventional and heat pump systems for energy and water conservation in open and confined greenhouse systems. Conventional gas furnaces and evaporative cooling, respectively, provided heating and cooling in the conventional system. In the heat pump systems, gas-fired heat pump units provided both heating and cooling. The greenhouse with heat pump units also had an option to be operated as a completely confined system, using one of the heat pump units as a dehumidifier. Average energy consumption in winter was 56.9, 23.5, and 11.3 MJ/day.m² in the conventional, …


Trees And Ice Storms: The Development Of Ice Storm–Resistant Urban Tree Populations (Second Edition), Richard J. Hauer, Jeffrey O. Dawson, Les P. Werner Jan 2006

Trees And Ice Storms: The Development Of Ice Storm–Resistant Urban Tree Populations (Second Edition), Richard J. Hauer, Jeffrey O. Dawson, Les P. Werner

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Severe ice storms occur every year in the United States and Canada, particularly in the midwestern and eastern regions of the United States. Along with fires and wind, ice storms are a frequent and major natural disturbance factor in eastern deciduous forests. Likewise ice storms are responsible for deaths and injuries of people and cause dramatic damage and tree loss to urban forests. Ice storms annually result in millions of dollars in loss, and potentially billions of dollars in losses for extreme and widespread ice storms. Damage to electric distribution systems, blocked roadways, and property damage from fallen trees and …


Dilthey And Simmel: A Reading From/Toward Buber's Philosophy Of History, Jules Simon Jan 2006

Dilthey And Simmel: A Reading From/Toward Buber's Philosophy Of History, Jules Simon

Selected Works Temporary Series

No abstract provided.


Hegels Familienbegriff – Vermittelt Durch Rosenzweig: Darstellung Einer Eigentuemlichen Geschichte, Jules Simon Jan 2006

Hegels Familienbegriff – Vermittelt Durch Rosenzweig: Darstellung Einer Eigentuemlichen Geschichte, Jules Simon

Selected Works Temporary Series

No abstract provided.


Dynamic Modeling Of Microclimate And Environmental Control Strategies In A Greenhouse Coupled With A Heat Pump System, Ilhami Yildiz, D. P. Stombaugh Jan 2006

Dynamic Modeling Of Microclimate And Environmental Control Strategies In A Greenhouse Coupled With A Heat Pump System, Ilhami Yildiz, D. P. Stombaugh

BioResource and Agricultural Engineering

The purpose of this study was to develop and validate a dynamic simulation model to be employed in accurate prediction of microclimate in a greenhouse as a function of dynamic environmental factors. The model has options to evaluate the effects of location, time of the year, orientation, single and double polyethylene glazings, conventional and heat pump heating and cooling systems, open and confined greenhouse systems, CO2 enrichment, variable shading, and the use of night curtains. Conventional gas furnace and evaporative cooling, respectively, provided heating and cooling in the conventional system. In the heat pump systems, gas-fired heat pump units …


Santa Clara Magazine, Volume 48 Number 3, Winter 2006, Santa Clara University Jan 2006

Santa Clara Magazine, Volume 48 Number 3, Winter 2006, Santa Clara University

Santa Clara Magazine

8 - WELCOME HOME, STEVE By Steven Boyd Saum. Basketball superstar Steve Nash '96 comes home to Santa Clara for a unique honor: a ceremony retiring his Bronco jersey. In a convocation address, he tells how Santa Clara changed his life. Now he wants the University to take its mission global.

12 - THE SCHOOL OF HOPE By Martha Ellen Stortz. Scholar and teacher Bill Spohn earned deep affection and the respect of the Santa Clara community in his years directing the Bannan Center for Jesuit Education. When he was stricken with cancer, he and his wife, Marty Stortz, looked …


Disinfection Of Swine Wastewater Using Chlorine, Ultraviolet Light And Ozone, John J. Macauley, Zhimin Qiang, Rao Y. Surampalli, Melanie R. Mormile, Craig D. Adams Jan 2006

Disinfection Of Swine Wastewater Using Chlorine, Ultraviolet Light And Ozone, John J. Macauley, Zhimin Qiang, Rao Y. Surampalli, Melanie R. Mormile, Craig D. Adams

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Veterinary antibiotics are widely used at concentrated animal feeding operations (CAFOs) to prevent disease and promote growth of livestock. However, the majority of antibiotics are excreted from animals in urine, feces, and manure. Consequently, the lagoons used to store these wastes can act as reservoirs of antibiotics and antibiotic-resistant bacteria. There is currently no regulation or control of these systems to prevent the spread of these bacteria and their genes for antibiotic resistance into other environments. This study was conducted to determine the disinfection potential of chlorine, ultraviolet light and ozone against swine lagoon bacteria. Results indicate that a chlorine …


Meaningful Labeling Of Integrated Query Interfaces, Eduard Dragut, Clement Yu, Weiyi Meng Jan 2006

Meaningful Labeling Of Integrated Query Interfaces, Eduard Dragut, Clement Yu, Weiyi Meng

Cyber Center Publications

The contents of Web databases are accessed through queries formulated on complex user interfaces. In many domains of interest (e.g. Auto) users are interested in obtaining information from alternative sources. Thus, they have to access many individual Web databases via query interfaces. We aim to construct automatically a well-designed query interface that integrates a set of interfaces in the same domain. This will permit users to access information uniformly from multiple sources. Earlier research in this area includes matching attributes across multiple query interfaces in the same domain and grouping related attributes. In this paper, we investigate the naming of …


Vertical Loads Due To Wheat On Obstructions Located On The Floor Of A Model Bin, Marek Molenda, Michael D. Montross, Sidney A. Thompson, Jozef Horabik Jan 2006

Vertical Loads Due To Wheat On Obstructions Located On The Floor Of A Model Bin, Marek Molenda, Michael D. Montross, Sidney A. Thompson, Jozef Horabik

Biosystems and Agricultural Engineering Faculty Publications

Tests were conducted in a model grain bin to evaluate the vertical loads acting on differently shaped obstructions embedded in wheat during filling, detention, and discharge. The bin had corrugated galvanized steel walls with a 1.83 m diameter and a flat bottom. All tests were conducted in a bin that was centrically loaded and unloaded. Three differently shaped obstructions (disc, cone, and cylinder) were tested; each had a circular base equivalent to 6% of the bin floor area. The obstructions were supported in the bin using a three-legged support structure. Each leg of the support structure rested on a load …


Design And Implementation Of A Storage Management Method For Content Distribution, Chang-Soo Kim, Yu-Hyeon Bak, Sang-Min Woo, Won-Jae Lee, Nunm-Ok Gee, Hag-Young Kim Jan 2006

Design And Implementation Of A Storage Management Method For Content Distribution, Chang-Soo Kim, Yu-Hyeon Bak, Sang-Min Woo, Won-Jae Lee, Nunm-Ok Gee, Hag-Young Kim

Electrical and Computer Engineering Faculty Research & Creative Works

The SMART system is a special purpose server developed by ETRI and designed for efficient streaming services over high speed networks. The SMART server has one or more special purpose NS (Network-Storage) card. The NS card has several disks that store multimedia contents. However all of the multimedia contents to be serviced cannot be stored at the server. In this paper, we will describe the storage management mechanism in design and implementation aspects. With this storage management mechanism, the SMART server can provide effectiveness in managing storage and distributing some contents from a source station to streaming service servers.


Nanoelectropulse-Driven Membrane Perturbation And Small Molecule Permeabilization, P. Thomas Vernier, Yinghua Sun, Martin A. Gundersen Jan 2006

Nanoelectropulse-Driven Membrane Perturbation And Small Molecule Permeabilization, P. Thomas Vernier, Yinghua Sun, Martin A. Gundersen

Bioelectrics Publications

Background
Nanosecond, megavolt-per-meter pulsed electric fields scramble membrane phospholipids, release intracellular calcium, and induce apoptosis. Flow cytometric and fluorescence microscopy evidence has associated phospholipid rearrangement directly with nanoelectropulse exposure and supports the hypothesis that the potential that develops across the lipid bilayer during an electric pulse drives phosphatidylserine (PS) externalization.

Results
In this work we extend observations of cells exposed to electric pulses with 30 ns and 7 ns durations to still narrower pulse widths, and we find that even 3 ns pulses are sufficient to produce responses similar to those reported previously. We show here that in contrast to …