Engineering Commons^™

Plasma Membrane Voltage Changes During Nanosecond Pulsed Electric Field Exposure, W. Frey, R. O. Price, P. F. Blackmore, R. P. Joshi, R. Nuccitelli, S. J. Beebe, K. H. Schoenbach, J. F. Kolb

Bioelectrics Publications

The change in the membrane potential of Jurkat cells in response to nanosecond pulsed electric fields was studied for pulses with a duration of 60 ns and maximum field strengths of similar to 100 kV/cm (100 V/cell diameter). Membranes of Jurkat cells were stained with a fast voltage-sensitive dye, ANNINE-6, which has a subnanosecond voltage response time. A temporal resolution of 5 ns was achieved by the excitation of this dye with a tunable laser pulse. The laser pulse was synchronized with the applied electric field to record images at times before, during, and after exposure. When exposing the Jurkat …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2005, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2005 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award in a single technical report that includes: 1) a synopsis of each research project supported during the period, 2) related reports and publications, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.

Diverse Effects Of Nanosecond Pulsed Electric Fields On Mammalian Cells, Jody Anne White

Theses and Dissertations in Biomedical Sciences

The continuing effort to manipulate cell-signaling pathways for therapeutic benefit has lead to the exploration of electric field effects on cells. Current electric field applications include electroporation of the plasma membrane for introduction of drugs, genes, or other macromolecules into cells. Modeling of how these pulsed electric fields affect cells depicts the cell as an excitable circuit. In this model, the electric fields, administered in short pulses to a cell, charge the plasma and internal membranes, which act as dielectric layers, and between these the cytoplasm acts as a conductive medium. The pulse lengths of this treatment are traditionally in …

The Journal Of Undergraduate Research: Volume 04

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 4.

Gis And 3d Analysis Applied To Sea Turtle Mortalities And Navigation Channel Dredging, Bradley A. Shellito, Keith B. Lockwood

Virginia Journal of Science

Between 2000 and 2003 there were an increased number of documented sea turtle mortalities related to hopper dredging in the channels of the Chesapeake Bay. A pilot study was undertaken to create a bathymetric surface and three-dimensional model of the Cape Henry Channel using Geographic Information Systems (GIS) as a visualization tool to examine sea turtle mortalities in relation to the dredging. In Fall 2003, the US Army Corps of Engineers dredged the Thimble Shoals Federal Navigation Channel, and a more refined model was developed using this data. This project examines the growing concerns over sea turtle mortality rates and …

Air Exchange Rate In A Horse Trailer During Road Transport, Joseph L. Purswell, Richard S. Gates, Laurie M. Lawrence, Jamey D. Jacob, Timothy S. Stombaugh, Robert J. Coleman

Biosystems and Agricultural Engineering Faculty Publications

Horses traveling by road commonly experience heat stress conditions and poor air quality, which may be caused by insufficient ventilation; however, there are few estimates of air exchange in a horse trailer during transport. Air exchange rate was measured at ten locations within a four-horse trailer (internal volume 18.5 m³) using tracer gas decay measurement to assess the adequacy of ventilation. Three vehicle speeds (13, 48, and 97 km h^-1) and three window configurations (all windows and roof vents closed, all windows open, all windows open and roof vents open forward) were tested with and without …

System Identification Of A Pasteurisation Process: A Comparison Of Direct And Indirect Methods, Niel Canty

Theses

The primary focus of this research is to identify a linear model for a direct steam injection (DSI) pasteurisation process which is a component of a large-scale pilot evaporation process widely used in the dairy industry. The pasteurisation process has been identified as a key component of the evaporation process. The author believes that no previously published work has focused on identifying models for a DSI pasteurisation process which makes this work an original contribution to the area. The pasteurisation process can be modelled as a single-input, single-output (SISO) process with a measurable disturbance. Thus two transfer functions were required …

Synthesising Life-Sciences And Control Engineering: An Outline Survey And A Practical Example, Aidan O'Dwyer

Conference papers

There is an increasing interest in life science related areas in the electrical and control engineering community [1]. One manifestation of this interest is the development of interdisciplinary programmes, such as the B.Sc. in Medical Physics and Bioengineering, offered by the Dublin Institute of Technology from September 2005. A significant amount of research work in control engineering has focused on aspects of human physiological system, for example blood glucose regulation [2], cardiovascular modelling and control [3], and limb control [4]. There is an increasing emphasis on interdisciplinary research; in recognition of this, the Institute of Electrical Engineers (IEE) has starting …

Bayer Ag V. Housey Pharmaceuticals: Protection For Biotechnological Research Tools Under Section 271(G) Found Wanting, Matthew Barthalow

The University of New Hampshire Law Review

[Excerpt] "Research tools, a subset of biotechnological inventions protected by process patents, are “tools that scientists use in the laboratory, including cell lines, monoclonal antibodies, reagents, animal models, growth factors, combinatorial chemistry and DNA libraries, clones and cloning tools (such as PCR), methods, laboratory equipment and machines.” Many companies base their business models on the ability to find pharmaceutical products using their proprietary drug discovery research tools. Research tools used for drug discovery ‘include bioinformatic methods for identifying the interaction of certain proteins and their association with disease, methods for confirming protein targets, screening assays to identify molecules active against …

Distribution Pattern Variability Of Granular Vrt Applicators, John P. Fulton, Scott A. Shearer, Stephen F. Higgins, Dennis Wayne Hancock, Timothy S. Stombaugh

Biosystems and Agricultural Engineering Faculty Publications

Granular applicators equipped with variable-rate technology (VRT) have gained popularity in recent years as a result of increased interest in variable-rate application. The purpose of this investigation was to characterize distribution patterns at varying rates for different granular applicators. Uniform-rate (UR) tests were conducted to assess the accuracy of variable-rate application from four granular applicators: two spinner-disc spreaders (A and B), and two pneumatic applicators (C and D). Pattern results indicated a consistent triangular pattern for spinner spreader B and consistent patterns for the pneumatic applicators (C and D). However, applicator D produced pattern variations at the center and right …

Rate Response Assessment From Various Granular Vrt Applicators, John P. Fulton, Scott A. Shearer, Stephen F. Higgins, Matthew J. Darr, Timothy S. Stombaugh

Biosystems and Agricultural Engineering Faculty Publications

Variable-rate technology (VRT) adds complexity to application equipment, thereby confounding the assessment of applicator performance. The intent of this investigation was to assess the rate response of various VRT granular applicators: two spinner spreaders (A and B), and two pneumatic applicators (C and D). Variable-rate (VR) tests were conducted to quantify the rate response characteristics (delay and transition times) for the applicators. A sigmoidal function was used to model the rate response for five of the six tests. Applicator A exhibited a linear response during decreasing rate changes. Results indicated that only applicator B demonstrated consistent delay and transition times, …

2005 Scholars And Artists Bibliography, Stephen D. Slane Dr., Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Dr, Steve Slane was the guest speaker.

Santa Clara Magazine, Volume 47 Number 2, Fall 2005, Santa Clara University

Santa Clara Magazine

6 - THE DRIVE TO CREATE by Elizabeth Kelley Gillogly '93. Albert Hoagland, an adjunct professor at SCU for more than 20 years, helped to build the first disk drive. Now is he working to preserve the history of magnetic disk storage.

10 - UNFINISHED MESSAGE by Toshio Mori. We share an excerpt from a collection of short stories by Mori, whose work highlights the plight of Japanese immigrants in the U.S. during World War II. The book is a part of the California Legacy Series, a partnership between SCU and Heyday Books.

14 - THE FAMILIAR STRANGER By Cynthia …

Structure Of Human Thymidylate Synthase Under Low-Salt Conditions, Leslie L. Lovelace, Wladek Minor, Lukasz Lebioda

Faculty Publications

Human thymidylate synthase, a target in cancer chemotherapy, was crystallized from PEG 3350 with 30 mM ammonium sulfate (AS) in the crystallization medium. The crystals are isomorphous with the high-salt crystals (~2.0 M AS) and the structure has been solved and refined (R = 22.6%, Rfree = 24.3%) at 1.8 Å resolution. The high- and low-AS-concentration structures are quite similar, with loop 181-197 is in the inactive conformation. Also, residues 95-106 and 129-135 (eukaryotic inserts region) show high mobility as assessed by poor electron density and high values of crystallographic temperature factors (residues 1-25 and 108-129 are disordered in both …

Sheep Updates 2005 - Part 2, Peter Tozer, Janette Drew, K. E. Kemper, M. L. Hebart, F. D. Brien, K. S. Jaensch, R. J. Grimson, D. H. Smith, A. M. M. Ramsay, J. S. Richards, K. D. Atkins, T. Thompson, W. K. Murray, J. R. L. Hall, Chris Carter, Peter Tozer, Mark Hyder, John Young, B. A. Mcgregor, Patricia Harper

Sheep Updates

This session covers seven papers from different authors: CONCURRENT SESSIONS - STRATEGIC MANAGEMENT 1.Finishing Pastoral Lambs, Peter Tozer, Patricia Harper, Janette Drew, Department of Agriculture Western Australia 2. Coating Improves Wool Quality under Mixed Farming Conditions, KE Kemper, ML Hebart, FD Brien, KS Grimson, DH Smith AMM Ramsay, South Australian Research and Development Institute 3. J. S. Richards, K.D. Atkins, T. Thompson, W. K. Murray, Australian Sheep Industry Co-operative Research Centre and NSW Department of Primary Industries, Orange Agricultural Institute, Forest Rd. Orange 4. Strategic Risk Management in the Sheep Industry, J.R.L. Hall, ICON Agriculture (JRL Hall & Co) 5. …

Santa Clara Magazine, Volume 47 Number 1, Summer 2005, Santa Clara University

Santa Clara Magazine

9 - A MOGUL IN THE MAKING By Larry Sokoloff J.D. '92 While working toward his business degree, SCU sophomore Tyler Dickman runs a multimillion-dollar business based in Florida.

10 - TOP OF THE CLASS: FOUNDATION HONORS INNOVATIVE SCU PROFESSORS By Kim Kooyers. This year's winners of the Louis and Dorina Brutocao Award for Teaching Excellence and the Brutocao Family Foundation Award for Curriculum Innovation are examples of the SCU's outstanding teaching scholars.

14 - A GLOBAL ETHIC: A CONVERSATION WITH HANS KUNG By Rita Beamish '74 Hans Kung, scholar, Roman Catholic priest, and author of some 50 books on …

Spatial Variation Of Protein, Oil, And Starch In Corn, Samuel G. Mcneill, Michael D. Montross, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

Significant spatial yield variations are known to exist in cornfields with different soil types, topsoil depth, and other variables. Similarly, variations might also be found among the highly valued chemical components (oil, protein, and starch) in corn kernels due to local differences in soil type, fertility, acidity/pH, organic matter, etc. This study quantified the spatial variability of protein, oil, and starch content of corn from two conventional cornfields and two high-oil cornfields. Whole ears were harvested by hand from 20 to 40 randomly selected locations within each field. A differential global positioning system (DGPS) receiver recorded the location of each …

Dynamic Matrix Composition In Engineered Cartilage With Stochastic Supplementation Of Growth Factors, Asit K. Saha, J. (Jagannath) Mazumdar, Sean S. Kohles

Mechanical and Materials Engineering Faculty Publications and Presentations

Dynamic extracellular matrix (ECM) synthesis is explored in a hypothesized engineered cartilage construct. Growth (α) and decay (β) rate parameters are developed from a previous engineered cartilage model. The presented mathematical model was constructed from the parameterized experimental data using a deterministic and stochastic examination of ECM synthesis based on a negative feedback control mechanism. A growth factor supplementation is incorporated in a probabilistic mathematical approach. The growth factor component modified an initial deterministic model through a Gaussian white noise fluctuation. As the primary constituents of ECM, the mathematical tool is intended to characterize the probable steady state distribution of …

Cellulose Utilization By Clostridium Thermocellum: Bioenergetics And Hydrolysis Product Assimilation, Yi-Heng P. Zhang, Lee R. Lynd

Dartmouth Scholarship

The bioenergetics of cellulose utilization by Clostridium thermocellum was investigated. Cell yield and maintenance parameters, Y(X/ATP)True = 16.44 g cell/mol ATP and m = 3.27 mmol ATP/g cell per hour, were obtained from cellobiose-grown chemostats, and it was shown that one ATP is required per glucan transported. Experimentally determined values for G(ATP)P-T (ATP from phosphorolytic beta-glucan cleavage minus ATP for substrate transport, mol ATP/mol hexose) from chemostats fed beta-glucans with degree of polymerization (DP) 2-6 agreed well with the predicted value of (n-2)/n [corrected] (n = mean cellodextrin DP assimilated). A mean G(ATP)(P-T) value of 0.52 +/- 0.06 was calculated …

Validating Surface Energy Balance Fluxes Derived From Airborne Remote Sensing, José Luis Chávez Egüez

Biological Engineering

Remote sensing-derived energy balance components were compared against measured eddy covariance energy balance terms using heat flux source area models to validate the airborne multispectral remote sensing procedure in the estimation of instantaneous and daily evapotranspiration rates.

A procedure was developed to generate raster layers of the footprint weights for weighting/integrating the different components of the energy balance model and obtain meaningful comparisons to similar energy balance terms measured at eddy covariance and/or Bowen ratio stations.

Soil heat flux and surface aerodynamic temperature models were studied in an effort to improve the remote sensing estimation of distributed evapotranspiration rates. Aerial …

Permeability Of Corn, Soybeans, And Soft Red And White Winter Wheat As Affected By Bulk Density, Michael D. Montross, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

Darcy’s law is a function of viscosity, permeability, and velocity and can be used to predict the airflow resistance in granular materials at low air velocities. Permeability also governs the magnitude of natural convection currents during periods of non-aerated grain storage. The permeability of corn, soybeans, soft white winter wheat, and soft red winter wheat were measured as a function of bulk density and moisture content. Air was passed through a column of grain and the flow rate and pressure drop measured. Bulk density and kernel density were also measured to determine the porosity of grain in the test column. …

Airflow Resistance Of Seeds At Different Bulk Densities Using Ergun's Equation, Marek Molenda, Michael D. Montross, Samuel G. Mcneill, Jozef Horabik

Biosystems and Agricultural Engineering Faculty Publications

Airflow resistance of grains and oilseeds has been extensively studied. Traditionally the data has been presented using Shedd’s curves. However, this assumes that airflow resistance is independent of grain depth. Grain undergoes compaction during storage that changes the bulk density, porosity, and therefore the airflow resistance. Ergun’s equation is a function of particle size and porosity of the granular material. Airflow resistance by Ergun’s equation was used to predict the pressure drop across a column of corn, soft white winter wheat, soft red winter wheat, and soybeans at three moisture content levels and two bulk densities. The maximum root mean …

Crop Yield Response To Precision Deep Tillage, Larry G. Wells, Timothy S. Stombaugh, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

Experimental precision deep tillage was applied at three sites in central Kentucky with relatively well-drained silt loam soils in no-till crop production. Fields were divided into 0.4 ha (1 ac) grid cells using DGPS mapping. Assessment of soil compaction by machinery traffic was made using multiple soil cone penetrometer measurements and expressed as cone index (CI). Corn, wheat, and soybean yields were depressed in grid cells with CI_avg ≥ 1.5 MPa (218 psi) prior to application of tillage treatments at sites 1 and 3, whereas at site 2, where most of the highest average CI values ranged from 1.44 …

Effect Of Fat And Casein Particles In Milk On The Scattering Of Elliptically Polarized Light, Czarena L. Crofcheck, Jeremie Wade, Janakiraman N. Swamy, Mustafa M. Aslan, M. Pinar Mengüç

Biosystems and Agricultural Engineering Faculty Publications

In this article, we present an experimental approach to determine the milk fat content using scattered light intensity profiles. The elements of the scattering (Mueller) matrix have been shown to provide valuable information about variation of the optical properties of scattering particles. The scattering behavior of fat and casein in terms of the scattering matrix elements was experimentally determined for milk with varying fat levels ranging from 0.05 wt% (skim) to 3.20 wt% (whole). Three of the scattering Mueller matrix elements, specifically S₁₁, S₁₂/S₁₁, and S₃₃/S₁₁, were found to be …

Glucose Oxidase (God)-Coupled Amperometric Microsensor With Integrated Electrochemical Actuation System, Jongwon Park, Chang-Soo Kim, Minsu Choi, Shanrui Zhang

Electrical and Computer Engineering Faculty Research & Creative Works

Recent developments for biosensors have been mainly focused on miniaturization and exploratory use of new materials. It should be emphasized that the absence of a novel "in-situ self-calibration/diagnosis technique" that is not connected to an external apparatus is a key obstacle to the realization of a biosensor for continuous use with minimum attendance. In order to address this issue, a novel solid-state glucose oxidase-coupled amperometric biosensor with integrated electrochemical actuation system has been designed and validated. There are two key components of the proposed glucose biosensor: solid-state GOD-coupled thin-lm amperometric sensing element and O₂ depleting/saturating built-in electrochemical actuator. The …

Microfluidics Fabrication And Testing For A Novel Biomems Cell Counting Device, Marly N. Dows

Honors Capstones

No abstract provided.

2005 - The Tenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Tenth Annual Symposium of Student Scholars, held on April 8, 2005. Includes abstracts from the presentations and posters.

Santa Clara Magazine, Volume 46 Number 4, Spring 2005, Santa Clara University

Santa Clara Magazine

8 - 'AN EXTRAORDINARY EVENT' By Elizabeth Kelley Gillogly '93. SCU Music Professor Hans Boepple is a gifted pianist and dedicated teacher.

10 - EMBRACING DIFFERENCES By Adam Breen. Senior Aaron Uchikura spent a summer helping children in Moscow orphanages.

12 - THE KOKO CONNECTION By Victoria Hendel De La O. Senior Tierra Wilson's perseverance landed her a job as a gorilla research assistant and caregiver.

16 - A ROTATION IN ETHICS By Rita Beamish '74. Students learn about the ethics of health care by shadowing doctors and nurses at O'Connor Hospital in San Jose.

Identification And Expansion Of Pancreatic Stem/Progenitor Cells., You-Qing Zhang, Marcie Kritzik, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Pancreatic islet transplantation represents an attractive approach for the treatment of diabetes. However, the limited availability of donor islets has largely hampered this approach. In this respect, the use of alternative sources of islets such as the ex vivo expansion and differentiation of functional endocrine cells for treating diabetes has become the major focus of diabetes research. Adult pancreatic stem cells /progenitor cells have yet to be recognized because limited markers exist for their identification. While the pancreas has the capacity to regenerate under certain circumstances, questions where adult pancreatic stem/progenitor cells are localized, how they are regulated, and even …

Excavated Tanks And Hillside Dams, Martyn Keen

Natural resources published reports

An excavated tank is an earth structure on near level land used to impound water, in which a percentage of the storage capacity is below ground level. It includes similar structures constructed on sloping land, referred to as 'hillside dams'. These are specific types of the group of water-impounding structures known as 'farm dams'.