Engineering Commons^™

Drug Loading And Release From Polypeptide Multilayer Nanofilms, Yang Zhong

Doctoral Dissertations

Polypeptides, linear macromolecules, are formed from amino acid residues by linkage of peptide bonds. Proteins are polypeptides too, with more complex conformations contributing to specific functionalities. Disulfide bonds are very important to maintain the structure and functions of proteins, which will form between two cysteine (Cys) residues under oxidizing circumstance.

Cys containing polypeptides are designed and synthesized by F-moc (9-Fluorenylmethyloxycarbonyl) chemistry. The number and position of Cys residues can be controlled by amino acid sequences design and following peptide synthesis, which is important to gain insights on the nature of polyelectrolyte multilayer film assembly and stability.

Both commercial and designed …

Resistance Of The Target Islet Tissue To Autoimmune Destruction Contributes To Genetic Susceptibility In Type 1 Diabetes., Natasha J. Hill, Aleksandr Stotland, Michelle Solomon, Patrick Secrest, Elizabeth Getzoff, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Type 1 diabetes occurs when self-reactive T lymphocytes destroy the insulin-producing islet beta cells of the pancreas. The defects causing this disease have often been assumed to occur exclusively in the immune system. We present evidence that genetic variation at the Idd9 diabetes susceptibility locus determines the resilience of the targets of autoimmunity, the islets, to destruction. Susceptible islets exhibit hyper-responsiveness to inflammatory cytokines resulting in enhanced cell death and increased expression of the death receptor Fas. Fas upregulation in beta cells is mediated by TNFR2, and colocalization of TNFR2 with the adaptor TRAF2 in NOD beta cells is altered. …

Growth Of Mc3t3-El Pre-Osteoblasts On Borate Containing Glasses & Growth Of Osteoblastic Cells On 13-93 Glass Scaffolds, Agatha Dwilewicz

Opportunities for Undergraduate Research Experience Program (OURE)

Three borate-based glasses, compositions designated 1B, 2B, and 3B, with B₂0₃ levels of 15, 31, and 46 mole percent (Table 1), respectively, were tested for their effects on the growth of MC3T3-El mouse pre-osteoblastic cells under physiological fluid conditions. Silicate type 45S5 glass was used as a control for comparison. Independently, the biocompatibility of recently developed silicate-based porous 13-93 bioactive glass scaffolds was tested; Saos-2 human osteosarcoma cell line was used. Tests performed for the two studies included: contact assays of cell growth at the glass interface, chemical analysis of borate released into the culture medium, quantitative …

Process Development For The Formation Of Post-Bonding Biorecognition Layers In Microfluidic Biosensors, Martin G. Perez, Phaninder R. Kanikella, James N. Reck, Chang-Soo Kim, Matthew O'Keefe

Electrical and Computer Engineering Faculty Research & Creative Works

Formation of the biorecognition layers within microfluidic sensor channels must be done after the completion of the channel structure since these layers cannot withstand the wafer bonding temperature. We propose a new post-bonding immobilization process to prepare the enzyme layers within microfluidic channels of electrochemical biosensors. An array of Pt vertical electrodes is electroplated using a SU-8 mold. The cured SU-8 is then removed by plasma etching to expose the Pt electrode and to define the fluidic channel cavity simultaneously. An array of enzyme posts is formed on the Pt surface by either electropolymerizing or photopolymerizing enzyme precursor solutions injected …

Self-Consistent Analyses For Potential Conduction Block In Nerves By An Ultrashort High-Intensity Electric Pulse, R. P. Joshi, A. Mishra, Q. Hu, K. H. Schoenbach, A. Pakhomov

Bioelectrics Publications

Simulation studies are presented that probe the possibility of using high-field (>100kV ∕ cm), short-duration (∼50ns) electrical pulses for nonthermal and reversible cessation of biological electrical signaling pathways. This would have obvious applications in neurophysiology, clinical research, neuromuscular stimulation therapies, and even nonlethal bioweapons development. The concept is based on the creation of a sufficiently high density of pores on the nerve membrane by an electric pulse. This modulates membrane conductance and presents an effective "electrical short" to an incident voltage wave traveling across a nerve. Net blocking of action potential propagation can then result. A continuum approach based …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2006, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2006 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award into a single techincal report that includes: 1) a synopsis of each student research enhancement project conducted during the period, 2) citations for related reports and presentations, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, 5) notable awards and achievements during the year, and 6) publications from prior projects.

The Journal Of Undergraduate Research: Volume 05

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 5.

Dust Emission From South Dakota Cattle Feedlots, Rick Weber

The Journal of Undergraduate Research

Dust and particle emissions (PM) from cattle feedlots is a concern for producers in order to maintain good relations with neighbors and also to comply with the EPA proposed PM_2.5 particulate emission rules. Dust at 2.5 microns, which cannot be seen by the naked eye, is a potential health hazard since it can be ingested into the lungs of humans. One of the proposed regulations of the Federal Clean Air Act is to apply the PM_2.5 standard to the agricultural sector. This research project examines the particulate matter dust emissions of various sizes in microns including 2.5, 10, …

Whole Genome Duplications And Contracted Breakpoint Graphs, Max A. Alekseyev, Pavel A. Pevzner

Faculty Publications

The genome halving problem, motivated by the whole genome duplication events in molecular evolution, was solved by El-Mabrouk and Sankoff in the pioneering paper [SIAM J. Comput., 32 (2003), pp. 754–792]. The El-Mabrouk–Sankoff algorithm is rather complex, inspiring a quest for a simpler solution. An alternative approach to the genome halving problem based on the notion of the contracted breakpoint graph was recently proposed in [M. A. Alekseyev and P. A. Pevzner, IEEE/ACM Trans. Comput. Biol. Bioinformatics, 4 (2007), pp. 98–107]. This new technique reveals that while the El-Mabrouk–Sankoff result is correct in most cases, it does not hold in …

Colored De Bruijn Graphs And The Genome Halving Problem, Max A. Alekseyev, Pavel A. Pevzner

Faculty Publications

Breakpoint graph analysis is a key algorithmic technique in studies of genome rearrangements. However, breakpoint graphs are defined only for genomes without duplicated genes, thus limiting their applications in rearrangement analysis. We discuss a connection between the breakpoint graphs and de Bruijn graphs that leads to a generalization of the notion of breakpoint graph for genomes with duplicated genes. We further use the generalized breakpoint graphs to study the Genome Halving Problem (first introduced and solved by Nadia El-Mabrouk and David Sankoff). The El-Mabrouk-Sankoff algorithm is rather complex, and, in this paper, we present an alternative approach that is based …

Economic Analysis Of Cellulase Production Methods For Bio-Ethanol, Jun Zhuang, Mary A. Marchant, Sue E. Nokes, Herbert J. Strobel

Biosystems and Agricultural Engineering Faculty Publications

The cost of cellulase enzymes has limited the feasibility of producing ethanol from fibrous biomass. Traditional submerged fermentation (SmF) was compared to an alternative method of producing cellulase, solid state cultivation (SSC). Results from an economic analysis indicated that the unit costs for cellulase enzyme production were $15.67 (The prices are all 2004 prices in this article, except otherwise stated. We deflated newer prices to 2004 prices using a deflation factor 0.9 per year and inflated older prices to 2004 prices using an inflation factor 1.1.) per kilogram ($/kg) and $40.36/kg, for the SSC and SmF methods, respectively, while the …

Bioelectric Effects Of Intense Nanosecond Pulses, Karl H. Schoenbach, Barbara Y. Hargrave, Ravindra P. Joshi, Juergen F. Kolb, Richard Nuccitelli, Christopher J. Osgood, Andrei G. Pakhomov, Michael W. Stacey, James R. Swanson, Jody A. White, Shu Xiao, Jue Zhang, Stephen J. Beebe, Peter F. Blackmore, E. Stephen Buescher

Bioelectrics Publications

Electrical models for biological cells predict that reducing the duration of applied electrical pulses to values below the charging time of the outer cell membrane (which is on the order of 100 ns for mammalian cells) causes a strong increase in the probability of electric field interactions with intracellular structures due to displacement currents. For electric field amplitudes exceeding MV/m, such pulses are also expected to allow access to the cell interior through conduction currents flowing through the permeabilized plasma membrane. In both cases, limiting the duration of the electrical pulses to nanoseconds ensures only nonthermal interactions of the electric …

Biotechnology, Life Sciences And Control Engineering:Some Synthesis Issues, Aidan O'Dwyer

Conference papers

The synthesis of biotechnology, life sciences and control engineering is receiving increasing interest. The purpose of this contribution (and the associated poster) is to raise awareness of some of this synthesis work.

Improved Parameterization To Invert Rayleigh-Wave Data For Shallow Profiles Containing Stiff Inclusions, Carlos Calderon-Macias, Barbara Luke

Civil and Environmental Engineering and Construction Faculty Research

Inversion of shear-wave velocity profiles from phase-velocity measurements of Rayleigh-wave energy for sites containing stiff layers can be erroneous if such layers are not characterized in the starting or reference model. Incorporation of a priori knowledge then is key for converging upon a realistic or meaningful solution. Resolving soil profiles in desert regions where stiff layers cemented with calcium carbonate are intermixed with softer, uncemented media is an application for which locating shallow stiff inclusions has important implications. Identification of the stiff layers is critical for foundation design and cost estimating of excavations. A parameterization that seems adequate for this …

Masking Material As A Form Of Food Storage In Tiarinia Cornigera And Micippa Platipes (Brachyura, Majidae), J. Kyomo

Tanzania Journal of Science

Masking or decorating, which means attaching materials to the body, was investigated in two spider crabs Tiarinia cornigera and Micippa platipesin the subtropical waters of Okinawa, Japan. This life strategy was compared between sites over a period of time. All crabs of different ages and sizes attached materials, in one combination or the other, on their bodies proportionate to their carapace sizes. Older or larger crabs had their carapaces more completely covered with the materials than young or smaller crabs. Similar materials were found at all sites and had equal selective precedences by crabs. Red algae, particularly Gelidiella sp, was …

Hydrologic Properties Of Pervious Concrete, Joe D. Luck, Stephen R. Workman, Stephen F. Higgins, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

Pervious concrete is concrete made by eliminating most or all of the fine aggregate (sand) in the concrete mix, which allows interconnected void spaces to be formed in the hardened product. These interconnected void spaces allow the concrete to transmit water at relatively high rates. The main objective of this project was to conduct research on the potential application of pervious concrete in agricultural settings, specifically for use in animal feed lots, manure storage pads, animal manure and bedding compost facilities, or floor systems in animal buildings. Laboratory tests were conducted on replicated samples of pervious concrete formed from two …

Enzyme–Microbe Synergy During Cellulose Hydrolysis By Clostridium Thermocellum, Yanpin Lu, Yi-Heng P. Zhang, Lee R. Lynd

Dartmouth Scholarship

Specific cellulose hydrolysis rates (g of cellulose/g of cellulase per h) were shown to be substantially higher (2.7- to 4.7-fold) for growing cultures of Clostridium thermocellum as compared with purified cellulase preparations from this organism in controlled experiments involving both batch and continuous cultures. This “enzyme–microbe synergy” requires the presence of metabolically active cellulolytic microbes, is not explained by removal of hydrolysis products from the bulk fermentation broth, and appears due to surface phenomena involving adherent cellulolytic microorganisms. Results support the desirability of biotechnological processes featuring microbial conversion of cellulosic biomass to ethanol (or other products) in the absence of …

2006 Scholars And Artists Bibliography, Daniel J. Simon, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library

Scholars and Artists Bibliographies

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Dr. Dan Simon was the guest speaker.

Santa Clara Magazine, Volume 48 Number 2, Fall 2006, Santa Clara University

Santa Clara Magazine

8 - TRUTH, LEGEND, AND JESSE JAMES By Ron Hansen M.A. '95. Jesse James' exploits made him a legend even in his own time. Now the author of the novel The Assassination of Jesse James by the Coward Robert Ford reveals what it takes to get beyond coloring book heroes and villains to understanding a charming psychopath and his killer. Plus insights into the making of the forthcoming film starring Brad Pitt and Casey Affleck.

14 - VIOLENCE IN THE MOVIES By Jim Shepard. Movies keep giving us more motion, more mayhem-which is exactly what we want. But what price …

Fisheries Research Contract Report No. 14 - Aquatic Animal Health Subprogram: Development Of A National Translocation Policy Using Abalone And Prawns As Templates For Other Aquatic Species (Frdc Project No. 2004/080) Final Report, Brian Jones, Fran Stephens

Fisheries Research Contract Reports

The use of scientifically based hazard identification, risk analysis and risk management is fundamental to managing unwanted effects. Hazards, such as quarantine incursions, will continue to occur despite the use of risk assessment methodologies but the frequency and severity of their occurrence will be reduced.

This was recognised by the Ministerial Council on Forestry, Fisheries and Aquaculture in 1999 when they published the "National policy for the translocation of live aquatic organisms".

This FRDC project provides a simple risk assessment methodology based on the Australian Standard for Risk Management (AS/NZS 4360: 2004) and one that is consistent with the Ministerial …

The Effects Of Ultrashort Pulsed Electric Fields On The Fluorescent Molecule Bodipy Fl C5 Ceramide, Rachael Sara Shevin

Electrical & Computer Engineering Theses & Dissertations

Nanosecond pulsed electric fields are able to manipulate internal cell organelles by taking advantage of the small but finite time required for charge to establish across a capacitive barrier. Pulsed electric fields also affect macromolecular structures such as enzymes and proteins. Application of a single nanosecond pulsed electric field decreases the fluorescence of a commercially available biological dye Bodipy FL C₅ Ceramide, used to observe the Golgi apparatus of living cells. The effect is seen when the fluorophore is exposed to an electric field both in vitro and when incorporated into the Golgi of a living Jurkat cell. Observation …

Oxidase-Coupled Amperometric Glucose And Lactate Sensors With Integrated Electrochemical Actuation System, Jongwon Park, Chang-Soo Kim, Minsu Choi

Electrical and Computer Engineering Faculty Research & Creative Works

Unpredictable baseline drift and sensitivity degradation during continuous use are two of the most significant problems of biosensors including the amperometric glucose and lactate sensors. Therefore, the capability of on-demand in situ calibration/diagnosis of biochemical sensors is indispensable for reliable long-term monitoring with minimum attendance. Another limitation of oxidase enzyme-based biosensors is the dependence of enzyme activity on the background oxygen concentration in sample solution. In order to address these issues, the electrolytic generation of oxygen and hydrogen bubbles were utilized 1) to overcome the background oxygen dependence of glucose and lactate sensors and 2) to demonstrate the feasibility of …

Santa Clara Magazine Volume 48 Number 1, Summer 2006, Santa Clara University

Santa Clara Magazine

8 - THE MAN BEHIND THE SOUND by Karen Crocker Snell. As a young music-loving soldier in the final days of World War II, John T. "Jack" Mullin '36 went to investigate a German recording device called a magnetophon. His resulting work in sound profoundly affected the field of recorded audio.

14 - THREE ROOMMATES IN PARIS By John Patrick Donnelly, S.J. It has been 500 years since Francisco Xavier and Pierre Favre were born, and 450 years since the death of Ignatius of Loyola. Quite a lot has happened since 1529 at the University of Paris, when the three …

Changing Maine, 1960-2010: Teaching Guide, Richard Barringer, New England Environmental Finance Center

Maine History & Policy Development

Unlike forty years ago, none of us is now certain what the future holds for Maine – except that it will be different. Maine has been transformed by the events of the recent decades. We have come into a new world, a new time – a new historical era, if you will. This new era, like previous eras in Maine history, will require of us new ways of thinking, new ways of understanding, new ways of organizing ourselves as a community of people, if the values and culture we share and cherish are to endure and flourish.

Molecular Modeling Of Proteins And Peptides Related To Cell Attachment In Vivo And In Vitro, Wanhua Zhao

Doctoral Dissertations

Polypeptides constitute half of the dry mass of the cell, they form the bulk of the extracellular matrix (ECM), and they are a common element of extra- and intracellular signaling pathways. There is increasing interest in the development of computational methods in polypeptide and protein engineering on all length scales. This research concerns the development of computational methods for study of polypeptide interactions related to cell attachment in vivo and in vitro.

Polypeptides are inherently biocompatible, and an astronomical range of unique sequences can be designed and realized in massive quantities by modern methods of synthesis and purification. These …

The Land Is In Your Hands : A Practical Guide For Owners Of Small Rural Landholdings In Western Australia, Department Of Agriculture And Food, Wa

Bulletins 4000 -

This Bulletin discusses various aspects of managing a small farm or property in Western Australia, including soil and land care, vegetation and plant control on farm holdings, water resource management, biosecurity, plant, animal and insect pest control and livestock management.

Development And Testing Of A Low-Cost Condensation Detection System, Michael D. Montross, George A. Duncan, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

A condensation sensing and control system was designed to detect condensation using a commercially available leaf wetness sensor (LWS). The leaf wetness sensor was a variable resistance grid-type that responded to moisture on the surface. A circuit was developed to compare the LWS voltage output to a user specified reference voltage, and operate a relay for possible switching of a humidity control device (for example a fan and/or heater). The condensation detection system operation was validated in an environmental chamber in the laboratory using a heat exchanger and water bath. Condensate was immediately detected when the plate was cooled below …

A Short Tutorial On Reconstructability Analysis, Martin Zwick

Complex Systems Faculty Publications and Presentations

This talk will present the basic ideas of Reconstructability Analysis. RA is a general modeling methodology developed in the systems community since the early 60s (pioneered by Ross Ashby) for exploratory (data mining, machine learning) and confirmatory modeling. RA is based in graph theory, set theory, information theory, and statistics. It is applicable to qualitative (nominal) and, via binning, to quantitative (continuous) data, with both statistical & non-statistical applications. It overlaps and augments more widely known methods, e.g., log-linear modeling and Bayesian networks.

Keywords: Reconstructability Analysis, Log-Linear Modeling, categorical data, multivariate statistics, exploratory modeling, confirmatory modeling

Dissociation Of Automatic And Strategic Lexical-Semantics: Functional Magnetic Resonance Imaging Evidence For Differing Roles Of Multiple Frontotemporal Regions, Brian T. Gold, David A. Balota, Sara J. Jones, David K. Powell, Charles D. Smith, Anders H. Andersen

Neuroscience Faculty Publications

Behavioral research has demonstrated three major components of the lexical-semantic processing system: automatic activation of semantic representations, strategic retrieval of semantic representations, and inhibition of competitors. However, these component processes are inherently conflated in explicit lexical-semantic decision tasks typically used in functional magnetic resonance imaging (fMRI) research. Here, we combine the logic of behavioral priming studies and the neurophysiological phenomenon of fMRI priming to dissociate the neural bases of automatic and strategic lexical-semantic processes across a series of three studies. A single lexical decision task was used in all studies, with stimulus onset asynchrony or linguistic relationship between prime and …

Desulfovibrio Desulfuricans G20 Tetraheme Cytochrome Structure At 1.5 A˚ And Cytochrome Interaction With Metal Complexes, Mrunalini Pattarkine, J J. Tanner, C A. Bottoms, Y H. Lee, Judy D. Wall

Faculty Works

The structure of the type I tetraheme cytochrome c3 from Desulfovibrio desulfuricans G20 was determined to 1.5 A˚ by X-ray crystallography. In addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. Only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate. Alterations in the overall conformation and thermostability of the metal-oxidized protein were investigated …