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Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang 2014 University of Nebraska – Lincoln

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …


The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet 2014 Oklahoma State University

The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The floodplains of many gravel-bed streams have a general stratigraphy that consists of a layer of topsoil covering gravel-dominated subsoil. Previous research has demonstrated that this stratigraphy can facilitate preferential groundwater flow through focused linear features, such as paleochannels, or gravelly regions within the vadose zone. These areas within the floodplain vadose zone may provide a route for interactions between the floodplain surface and alluvial groundwater, effectively extending the hyporheic zone across the floodplain during high stream stage. The objective of this research was to assess the structure and scale of texture heterogeneity within the vadose zone within the gravel …


Geotextile Tubes And Their Application To Dewatering, Kevin R. Morgan 2014 Georgia Southern University

Geotextile Tubes And Their Application To Dewatering, Kevin R. Morgan

College of Graduate Studies: Theses & Dissertations

Geotextile tubes are a proven cost-effective technology offering speed, efficiency, and minimal impact to the environment for dewatering of sediment or sludge from dredging operations. Common applications include: removal of solids from municipal water and waste water treatment, agricultural remediation, marine dredging operations, construction dewatering, and beach preservation and protection. Geotextile tubes are manufactured in a variety of configurations.

Geotextile tubes are used for a wide variety of environmental applications including dewatering and slurry containment. One of the most important design aspects of the tubes themselves is the ability to be flexible and expand and hold form under high pressure …


Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier 2014 University of Nebraska-Lincoln

Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Communications, in general, involve delivery of information from a source to a sink. At nano-scale, an example of a man-made communications involving interfacing with biological systems at intra-cellular level is non-viral gene delivery. From a telecommunications engineering perspective, important end-to-end parameters of such a system are: the endto- end delay, system capacity, and packet loss rate. There are neither known methods to estimate those parameters theoretically nor they are ready available from standard measurements. The paper provides estimates for those parameters based on the simulation of non-viral gene delivery system based on the queuing theory. The simulator used has been …


Development Of Cementitious Materials For Adhesion Type Applications Comprising Calcium Sulfoaluminate (Csa) Cement And Latex Polymer, Joshua V. Brien 2014 University of Kentucky

Development Of Cementitious Materials For Adhesion Type Applications Comprising Calcium Sulfoaluminate (Csa) Cement And Latex Polymer, Joshua V. Brien

Theses and Dissertations--Civil Engineering

The objective of this research was to develop high performing polymer modified calcium sulfoaluminate (CSA) cement materials for use in applications requiring superior adhesion characteristics. Little information is available describing interactions of CSA cement containing minor phase tri-calcium aluminate (C3A) with commonly used admixtures. Given the scarcity of information, a basic approach for developing cementitious materials was followed. The basic approach consisted of four tasks: cement design, admixture design, polymer design and testing developed materials. The iterative, time consuming process is necessary for understanding the influence of specific constituent components on overall system behavior. Results from the cement …


Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier 2014 University of Nebraska-Lincoln

Dynamic Analysis Of Dna Nanoparticle Immobilization To Model Biomaterial Substrates Using Combinatorial Spectroscopic Ellipsometry And Quartz Crystal Microbalance With Dissipation, Tadas Kasputis, Alex Pieper, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within cells can be altered through gene delivery approaches, which have tremendous potential for gene therapy, tissue engineering, and diagnostics. Substrate-mediated gene delivery describes the delivery of plasmid DNA or DNA complexed with nonviral vectors to cells from a surface, with the DNA immobilized to a substrate through specific or nonspecific interactions. In this work, DNA-nanoparticle (DNA–NP) adsorption to substrates is evaluated using combinatorial, in situ spectroscopic ellipsometry and quartz crystal microbalance with dissipation (SE/QCM-D), to evaluate the basic dynamic processes involved in the adsorption and immobilization of DNA–NP complexes to substrates. The concentration of DNA–NP solutions influences …


Concise Handbook For Temporary Traffic Control, Indiana LTAP, Ohio Department of Transportation 2014 Lyles School of Civil Engineering

Concise Handbook For Temporary Traffic Control, Indiana Ltap, Ohio Department Of Transportation

Indiana Local Technical Assistance Program (LTAP) Publications

This handbook gives the basic principles and provides examples for the design, application, installation, and maintenance of the various types of traffic control devices used in temporary traffic control or for incident management. The guidelines presented are in accordance with the standards provided within the Indiana Manual for Uniform Traffic Control Devices (IMUTCD). This handbook is based upon one created by the Ohio Department of Transportation, Guidelines for Traffic Control in Work Zones. Indiana LTAP expresses thanks to ODOT for their permission to reprint this material.


Development Of Resilience Index In Transport Systems, Seungjae Lee, Jooyoung Kim, Shinhae Lee, Yan Chang-Richards 2014 University of Seoul

Development Of Resilience Index In Transport Systems, Seungjae Lee, Jooyoung Kim, Shinhae Lee, Yan Chang-Richards

International Institute for Infrastructure Resilience and Reconstruction (I3R2) Conference

This paper demonstrates the quantification of the resilience index (RI) in transport systems. The transport infrastructure can be managed by using the concepts of resilience. Vugrin, Warren, Ehlen, & Camphouse (2010) emphasized the enhancement of resilience in infrastructure before disasters and the establishment of efficient measures for the recovery of systems in an emergency. The concept of resilience has a significant influence on transport planning and operations for disaster preparation. Lee, Kim, & Lee (2013) investigated the concepts of resilience and examined case studies using valuable asset-management techniques in order to maintain the resilience concepts which should be introduced in …


Freeze Thaw Durability Of Internally Cured Concrete Made Using Superabsorbent Polymers, Wesley A. Jones, W. Jason Weiss 2014 Purdue University

Freeze Thaw Durability Of Internally Cured Concrete Made Using Superabsorbent Polymers, Wesley A. Jones, W. Jason Weiss

International Conference on Durability of Concrete Structures

The use of superabsorbent polymers (SAPs) to produce internally cured concrete has been shown to be effective in reducing the potential for restrained shrinkage cracking of high performance concrete mixtures. However, not much is known regarding the freeze–thaw durability of concrete mixtures that incorporate SAPs for internal curing (IC). When SAP particles desorb (or partially desorb) the “water” (pore fluid) they contain for the purposes of IC, some believe that the void space created by these particles can provide enough empty voids to accommodate the additional volume caused by water expansion upon freezing. This paper investigates the freeze–thaw durability of …


Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann 2014 University of Nebraska-Lincoln

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …


Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer 2014 University of Nebraska-Lincoln

Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Controller Area Network (CAN) bus is used on current agricultural equipment for in-vehicle communication among multiple microcontrollers. In this study, CAN data was collected from the tractor diagnostic port during three field operations: anhydrous ammonia (NH3) application, field cultivation, and planting. Specifically, CAN messages corresponding to engine fuel use rate were filtered, decoded, and analyzed. High fuel use rates were recorded from the tractor when implements were engaged in the soil compared to when the implements were lifted out of the ground at the end of the passes or, when the tractor was turning and idling. Threshold fuel …


Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah 2014 Wichita State University

Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral arterial disease (PAD) is characterized by atherosclerotic blockages of the arteries supplying the lower extremities, which cause a progressive accumulation of ischemic injury to the skeletal muscles of the lower limbs. This injury includes altered metabolic processes, damaged organelles, and compromised bioenergetics in the affected muscles. The objective of this study was to explore the association of Raman spectral signatures of muscle biochemistry with the severity of atherosclerosis in the legs as determined by the Ankle Brachial Index (ABI) and clinical presentation. We collected muscle biopsies from the gastrocnemius (calf muscle) of five patients with clinically diagnosed claudication, five …


Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang 2014 University of Nebraska-Lincoln

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …


Evaluation Of Long Term Effects Of Erosion Due To Groin Placement In The Northern Yucatan Peninsula, Andrew V. Mahfood 2014 Georgia Southern University

Evaluation Of Long Term Effects Of Erosion Due To Groin Placement In The Northern Yucatan Peninsula, Andrew V. Mahfood

College of Graduate Studies: Theses & Dissertations

This thesis documents the results of a study conducted to evaluate the long term effects of groin placement on beach re-nourishment and beach restoration in the Yucatan peninsula. The study includes the evaluation of six control points located on Chelem beach in Yucatan, Mexico, very close to the Progreso pier. The control points were set up in an active groin field to record data on the re-nourishment project covering the Chelem beach area. These erosion control groins were constructed as part of an experimental project to see how the adopted U.S. Army Corps of Engineers guidelines, for installing groins, affected …


Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller 2014 Pomona College

Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller

Pomona Senior Theses

This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.


Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet 2013 Rensselaer Polytechnic Institute

Elementary Implantable Force Sensor For Smart Orthopaedic Implants, Rebecca A. Wachs, David Ellstein, John Drazan, Colleen P. Healey, Richard L. Uhl, Kenneth A. Connor, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Implementing implantable sensors which are robust enough to maintain long term functionality inside the body remains a significant challenge. The ideal implantable sensing system is one which is simple and robust; free from batteries, telemetry, and complex electronics. We have developed an elementary implantable sensor for orthopaedic smart implants. The sensor requires no telemetry and no batteries to communicate wirelessly. It has no on-board signal conditioning electronics. The sensor itself has no electrical connections and thus does not require a hermetic package. The sensor is an elementary L-C resonator which can function as a simple force transducer by using a …


In-Plane Loading Of Brick Veneer Over Wood Shear Walls, Elias Anis Toubia, James M. Lintz 2013 University of Dayton

In-Plane Loading Of Brick Veneer Over Wood Shear Walls, Elias Anis Toubia, James M. Lintz

Civil and Environmental Engineering and Engineering Mechanics Faculty Publications

In the design of wood stud walls with brick veneer, current design building codes specify that the wood stud wall should resist all in-plane and out-of-plane loads (IBC 2009). For out-of-plane loads, this assumption is entirely justified as the brittle brick veneer will crack and lose its capacity to resist bending. For in-plane loads, the brick veneer is significantly stiffer than the wood shear wall, and the veneer is unlikely to crack before the wood shear wall reaches its allowable capacity. The assumption that the wood shear wall resists the entire load is based on the further assumption that the …


A Simple Method For Estimation Of Queue Length, S. P. Anusha, Lelitha Devi Vanajakshi, Anuj Sharma 2013 Inidan Institute of Technology, Madras

A Simple Method For Estimation Of Queue Length, S. P. Anusha, Lelitha Devi Vanajakshi, Anuj Sharma

Department of Civil and Environmental Engineering: Faculty Publications

Urban arterials are characterized by frequent intersections. Queue length and delay are the two primary measures of performance of intersection. These measures play a primary role in determining the arterial performance. This article presents a methodology to determine the number of vehicles in queue at a signalized intersection for under saturated traffic conditions. The results obtained were validated using actual values that are manually extracted. The root-mean-square error is of the range 1.3 vehicles for estimation of number of vehicles in queue. The various aspects that have to be considered in accurate estimation of performance measures are also discussed.


Effects Of Waste Placement Practices On The Engineering Response Of Municipal Solid Waste, Jason Taylor Cox 2013 California Polytechnic State University, San Luis Obispo

Effects Of Waste Placement Practices On The Engineering Response Of Municipal Solid Waste, Jason Taylor Cox

Master's Theses

An extensive laboratory and field investigation was conducted at Santa Maria Regional Landfill (SMRL) in Santa Maria, CA to determine the effects of waste placement practices on the engineering response of municipal solid waste (MSW). Laboratory and field testing was used to determine the engineering properties and monitor field response of MSW.

The specific gravity (Gs) of manufactured MSW (MMSW), fresh MSW (FMSW), and old MSW (OMSW) was determined experimentally using a modified version of standard soil testing procedures. Effects of particle size, compactive effort, and degradation on the specific gravity of waste were evaluated. Specific gravity …


Absorption Simulation ( Absim ) Software Development, Vipul Vishnu Bhat, Ming Qu 2013 Purdue University

Absorption Simulation ( Absim ) Software Development, Vipul Vishnu Bhat, Ming Qu

The Summer Undergraduate Research Fellowship (SURF) Symposium

Researchers working on air conditioning systems currently use a software called ABSIM ( Absorption Simulation ) to evaluate current absorption systems or simulate new system designs. ABSIM was developed at Oak Ridge National Labs in the 1980s and the latest platform it is compatible with is Windows XP. ABSIM is not compatible on newer platforms and is not very user friendly. A lot of the actions involved in using ABSIM aren’t very intuitive. The goal is to develop a more user friendly simulation program that is based off of ABSIM and see to it that the program can be deployed …


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