Biology Commons^™

Upa-Mediated Polyamidoamine Dendrimer-Based Targeted Gene Delivery System On Triple-Negative Breast Cancer, Hsin-Yin Chuang

Masters Theses

"Around 15% of breast cancers are triple-negative breast cancer (TNBC), which is characterized by the absence of three common receptors—ER, PR, and HER2, and therefore do not respond to hormonal or anti-HER2 therapies. It is urgent to explore targeted therapeutic strategies for TNBC due to its poor prognosis and rare effective targeted therapy. In this study, we developed a polyamidoamine (PAMAM) dendrimer-based targeted gene delivery system—GDP-uPA, to utilize urokinase-type plasminogen activator (uPA) to target uPA receptor (uPAR), which is highly expressed in both TNBC cells and cancer-associated stromal cells. Our results of 1H NMR spectrum, TEM imaging, and MTT assay …

Elucidating The Priming Mechanism Of Clpxp Protease By Single-Domain Response Regulator Cpdr In Caulobacter Crescentus, Kimberly E. Barker

Masters Theses

In Caulobacter crescentus, progression through the cell cycle is regulated by the AAA+ protease ClpXP, and there are several classes of cell-cycle substrates that require adaptors in order to be degraded. CpdR, a single domain-response regulator, binds the N-terminal domain of ClpXP and primes the protease for degradation of downstream factors (Lau et al., 2015). The ability of CpdR to bind ClpX is regulated by its phosphorylation state. In the unphosphorylated state, CpdR binds ClpXP and guides its localization to the cell pole during the swarmer to stalked transition, where CpdR is mediates degradation of substrates such as PdeA. …

Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton

Masters Theses

No abstract provided.

Reintroducing Hemp (Rongony) In The Material Palette Of Madagascar: A Study On The Potential Of Hemp Clay Components And Its Impact On Social And Ecological Communities., Henintsoa Thierry Andrianambinina

Masters Theses

When mentioning the word hemp, especially in the local language of Madagascar, the literal translation does not set it apart from marijuana, as they are both called “rongony” - creating the stigma around hemp as the negative stereotype of marijuana. However, the material has been used by the ancestors of Madagascar, as well as across cultures, in its fibrous form to produce fabrication like textile goods and packaging. During colonization, the prohibition of hemp intensified, and since then, any activity related to either of these plants is prohibited and will end in severe punitive measures. This thesis explores the strengths …

Fungi In Flux | Designing Regenerative Materials And Products With Mycelium, Arvind Bhallamudi

Masters Theses

As the world grapples with the escalating crisis of climate threats and environmental degradation, this research delves into the synergistic potential of design and biology, developing safe and sustainable materials for applications in prototyping, furniture and interior design. Harnessing the power of a unique organism - fungi, the study proposes an accessible, efficient, and resilient material resource system. It utilizes local waste streams and mycelium (the vegetative part of fungi) to grow functional structures. An experimental and small-scale protocol is modeled by testing bio-fabrication and bio-printing methods. The composites' performance qualities and characteristics are evaluated through mechanical testing and a …

The Effects Of Anthropogenic Sensory Pollution On Arthropod Diversity And Pollinator Behavior, Sierra Dee Rodriguez, Jennifer N. Phillips Phd

Masters Theses

Pollinators provide a key ecological function in terrestrial ecosystems, yet in recent years, they have encountered unprecedented declines, likely due to anthropogenic change. Light and noise pollution, which can interfere with the visual and auditory systems of animals that regulate daily behaviors, are important factors to consider when communities are encroached by human development. While many researchers have looked at how vertebrate species behaviorally react to human caused habitat degradation and sensory pollution, little is known about how invertebrates, including arthropod pollinators, are affected, and whether there is a negative cascading effect on the plants that they pollinate. This research …

The Effects Of Urban Landscape, Soundscape, And Light Pollution On Avian Diversity And Nesting In South San Antonio, Texas, Alfredo Llamas

Masters Theses

As critical habitat continues to dwindle due to the expansion of urbanization, alternative habitat in urban ecosystems becomes a necessary natural resource for the survival of birds. In the last decade, local San Antonio environmental authorities have focused their efforts on restoring the San Antonio Mission Reach. Ecological revitalization of the San Antonio Mission Reach could increase avian, and flora biodiversity with ongoing restoration, yet how birds use restored habitat has not been assessed. Additionally, south of the Reach, two more peri-urban sites have great potential to be high quality habitat for birds but have not been surveyed for nesting …

The Application Of Microbial Source Tracking To Aid In Site Prioritization For Remediation In Lower Michigan, John J. Hart

Masters Theses

Non-point source fecal pollution is a threat to both the environment and public health. Climate change, aging infrastructure, and intensified agricultural practices are predicted to accentuate this issue. In Michigan, due to the high instance of aging infrastructure and intensified agriculture, non-point source fecal pollution has caused many waterbodies to exceed the state standards posing a risk to recreational activities and source water. Due to this threat, there is an increased effort to identify and remediate these sources. My study focused on improving the identification of non-point source fecal pollution through a combination of culture-based and molecular fecal indicator bacteria …

Species Responses To Habitat Edges And Fragmentation Per Se: A Cross Taxa Meta-Analysis, Rachel Snyder

Masters Theses

Aim We examined the scale equivalency of fragmentation effects at the patch scale and landscape scale to determine if patch level effects scale up to landscape level effects. First, we examined species responses to fragmentation effects at the patch scale and landscape scale. Second, we evaluated whether there is a difference in response between taxonomic groups and across latitudes. Finally, we analyzed the data at the level of individual species in order to determine how species respond to edge effects and fragmentation effects. Location Data for 71 studies were gathered from the BioFrag database. Studies used were located in 48 …

Climate Refugia For Neotropical Snakes: Implications Of Microhabitat Use And Diet Specialization, Consuelo Alarcon

Masters Theses

Aim: The aims of this study were to compare the locations of climate refugia and diversity patterns of the Neotropical tribe Pseudoboini and to provide critical analysis of the potential role of climatic stability and ecological specialization in the contemporary and historical distribution of this group of snakes. We expected species with narrow ecological requirements to occupy climatically stable areas, whereas generalists and arboreal species would occupy areas with more variable climate.

Location: South America

Methods: We compiled observations from the scientific literature and herpetological notes (1844-2019) with additional data from museum collections throughout South America to …

A Biodiversity Survey Of The Soil Crusts Of The Geographically Isolated San Nicholas Island, California, Usa And Description Of Species In Three Genera (Atlanticothix, Pycnacronema, Konicacronema) Previously Restricted To Brazil Using A Polyphasic Approach To Cyanobacterial Taxonomy, Brian Jusko

Masters Theses

San Nicholas Island, California, USA is a geographically-isolated island that experiences a semiarid climate and exhibits significant topographic and geologic diversity. Access to the island is restricted to the public and, as a result, only one previous study has been done on the algal biodiversity of its biological soil crusts. The previous study used morphology as the sole basis of species identification, and it was the aim of this study to corroborate and expand upon the results by including molecular data. Using 16S rRNA and 16S–23S ITS sequences and phylogenetic analyses, a diverse set of taxa were identified and are …

Wolbachia Infection In Gall Associated Insect Communities In Illinois And Indiana, Jakeb Watts

Masters Theses

Wolbachia is a maternally inherited, gram-negative bacterium and has been found to be associated with up to 60% of all insects, frequently resulting in male-killing, feminization, and cytoplasmic incompatibility in the hosts. Gall wasps and other gall-forming insects induce on various host plants abnormal tissue growths, or plant galls, with distinct morphology. They are considered as obligatory parasites to their host plant. The presence of Wolbachia infection in gall wasp populations in North America has not been extensively documented. Given the strong influence on host reproduction, not knowing Wolbachia infection in insect populations prevents the understanding of how populations are …

“Albertania” And “Egbenema”, Expanding Biodiversity In The Oculatellaceae (Cyanobacteria)., Mildred Akagha

Masters Theses

To conduct molecular studies of cyanobacteria of Lagos, Nigeria, a total of eight soil/subaerial samples were collected in Lagos State, Nigeria, and used to isolate cyanobacterial cultures that were characterized microscopically, sequenced, and phylogenetically analyzed. Within the resulting set of cultures, a number of Synechococcales were observed, particularly belonging to Oculatellaceae. “Egbenema” and two new putative species of the recently described genus Albertania were found. Both genera belong to a supported clade within the Oculatellaceae that includes Trichotorquatus and Komarkovaea. The two new putative species of Albertania, “A. egbensis” and “A. latericola”, were from the same sample, but phylogenetically were …

Mate Recognition In Genetically Distinct Populations Of The Eastern Red-Backed Salamander (Plethodon Cinereus), Courtney Thomas

Masters Theses

Lineages that have undergone genetic divergence and subsequently experience secondary contact present fascinating situations related to the potential for reproductive isolation. Within species hybrid zones provide unique opportunities to test the strength of reproductive barriers through behavioral experiments when genetic relatedness between groups is established. Among the complex interactions that operate to maintain species boundaries, mate recognition and mate choice are two behaviors that serve to promote evolutionary independence across diverse taxonomic lineages. I explored mate recognition in genetically distinct populations of the Eastern Red-backed Salamander, Plethodon cinereus, a small, abundant, terrestrial salamander with a widespread distribution throughout northeastern United …

Environmental Dna Metabarcoding As A Tool For Fish Community Assessment In Wetlands, Eric Ludwig

Masters Theses

"Environmental DNA (eDNA) metabarcoding is a genetically based method of assessing biodiversity in aquatic environments. While the efficacy of eDNA surveys has been well documented in riverine and marine systems, it has been relatively underemployed in freshwater wetland environments. In this study, we conducted an eDNA metabarcoding survey of fish diversity and its seasonal variation in a wetland along the Mississippi River in the Missouri Bootheel. Samples were collected from both permanent and seasonal water bodies including oxbow lakes, a shallow, man-made lake, a ditch, and a slough. For each of the 28 sites in this study, three water samples …

Benthic Macroinvertebrate Pre-Assessment Of A Proposed Restoration In The Grand River, Grand Rapids, Mi, Usa, Matthew L. Bain

Masters Theses

River restoration is the dominant field of applied water resources management in the United States. Ecological improvement should be the goal of all river restorations, though many restoration projects fail to produce positive results due to limited scope or inadequate assessment methodologies. Pre-restoration, biotic data is essential for such projects as it can be paired with post-restoration data to gauge ecological outcomes. A major restoration effort is now underway in Michigan's longest river, the Grand River, where it flows through downtown Grand Rapids. The primary restoration measures will occur in-stream at local scale. We conducted a pre- restoration survey for …

Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina

Masters Theses

Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their …

Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin

Masters Theses

Soil microbiomes play pivotal roles to the health of the environment by maintaining metabolic cycles. One question is how will climate change affect soil bacteria over time and what could the repercussions be. To answer these questions, the Harvard Forest Long-Term Warming Experiment was established to mimic predicted climate change by warming plots of land 5℃ above ambient conditions. In 2017, 14 soil core samples were collected from Barre Woods warming experiment to mark 15 years since the establishment of the soil warming in that location. These samples underwent traditional metatranscriptomics to generate an mRNA library as well as a …

Chemosensory Receptors In Berghia Stephanieae: Bioinformatics And Localization, Kelsi L. Watkins

Masters Theses

Chemosensation is achieved through the binding of chemical signals to chemoreceptor proteins embedded in the membranes of sensory neurons. The molecular identity of these receptors, as well as the downstream processing of chemosensory signals, has been well studied in arthropods and vertebrates. However, very little is known about molluscan chemosensation. The identity of chemoreceptor proteins in the nudibranch mollusc Berghia stephanieae are unknown. Data from other protostome and molluscan studies suggest Berghia may use ionotropic receptors for some forms of chemoreception. This study used a bioinformatics approach to identify potential chemosensory ionotropic receptors in the transcriptome of Berghia. A …

Influence Of Soil And Land Cover On Chronic Wasting Disease Prevalence In White-Tailed Deer And Mule Deer Across North America, Madison L. Miller

Masters Theses

Chronic wasting disease (CWD) is a fatal neurodegenerative disease that infects deer and is caused by a pathogenic prion. CWD is a concerning wildlife disease because it is incurable, potentially poses a risk to human health, and is spreading rapidly. CWD prions are transmitted both directly via bodily fluids and indirectly through environmental reservoirs such as soil. In this study, we investigated the influence of land cover and soil characteristics on CWD prevalence in white-tailed deer and mule deer. We acquired CWD prevalence data from seven North American regions and used ArcGIS to obtain land cover and soil characteristic data …

Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage

Masters Theses

Clarireedia spp. (formerly Sclerotinia homoeocarpaF.T. Bennett) is the causal agent dollar spot, the most economically important turfgrass disease impacting golf courses in North America. The most effective strategy for dollar spot control is repeated application of multiple classes of fungicides. However, reliance on chemical application has led to resistance to four classes of fungicides as well as multidrug resistance (MDR). Fungi are known to detoxify xenobiotics, like fungicides, through transcriptional regulation of three detoxification phases: modification, conjugation and secretion. Little is known, however, of the protein-protein interactions that facilitate these pathways. Following next-generation RNA sequencing of Clarireedia spp., a …

A Tipping Point In The Ecuadorian Amazon Rainforest: Current And Future Land-Use And Climate Change Trends, Alula Shields

Masters Theses

Many regions of the Amazon are experiencing drastic changes as deforestation and climate change drive the world’s largest continuous rainforest towards a ‘tipping point’. These disturbances are changing natural cycles that once past a critical threshold, will mark an unstoppable transition to an altered ecosystem. Losing areas of the Amazon rainforest will have implications for the global climate, global carbon budget, and global hydrological regimes. Scholars have projected these tipping points for areas of the eastern Amazon rainforest, but much less scholarship focuses on the headwaters of the Western Amazon, an area of great cultural and biological importance. Ecuador is …

Geographic Range Size As A Predictor Of Dispersal-Dependent Behavioral Traits In Two Clades Of A Terrestrial Salamander, Teah Evers

Masters Theses

Animal movement has the potential to affect diverse processes within ecology and evolution including range expansion, gene flow, adaptation, and speciation. Two aspects of animal personality that are germane to dispersal are exploratory and aggressive behavior. These behavioral categories may represent a trade-off such that energy invested in territorial defense leaves little energy for movement and dispersal. The Eastern Red-backed Salamander (Plethodon cinereus) is a wide ranging, dispersal limited, terrestrial salamander with well documented phylogeographic divisions. I examined dispersal-relevant behavioral traits within two clades of P. cinereus with disparate geographic ranges. The Northern Clade (NC) has a range extending from …

Survey Of Diatom Periphyton Assemblages In Lake George, Joy Jackson

Masters Theses

Lake George is an oligotrophic lake that has been monitored by the International Biological Monitoring Program (IBM) since the 1960s. The 51 km long lake is separated into two deep basins, one in the south where the major stream inputs are located, and one in the north where the lake feeds the La Chute River, which empties into Lake Champlain. The two basins are separated by a shallow channel named the “Narrows.” The southern basin has experienced a higher level of development than the northern basin. The aim of this study is to characterize the diatom assemblages in the northern …

Alkaloid-Based Chemical Defenses In 11 Species Of Bufonid Poison Frogs From Brazil (Bufonidae: Melanophryniscus), Emily Staufer

Masters Theses

The term ‘poison frog’ describes members of five anuran families that are capable of sequestering lipophilic, alkaloid-based chemical defenses from their prey. Alkaloid defenses can vary widely within and among poison frog species, mostly linked to differences in availability of arthropod prey at different scales. While research has mainly focused on alkaloid variation within the dendrobatid and mantellid poison frogs, bufonid poison frogs in the genus Melanophryniscus demonstrate high inter- and intra-species alkaloid variation, and some members are capable of both sequestering dietary alkaloids and synthesizing alkaloids metabolically. However, of the 31 species of Melanophryniscus, only eight have ever had …

Assessment Of Repeatability And Behavioral Syndromes In Genetically Distinct Clades Of Eastern Red-Backed Salamanders (Plethodon Cinereus), Kelsey Garner

Masters Theses

Animal personality implies limited behavioral plasticity and behavioral traits which are correlated across contexts. Correlated behaviors, sometimes referred to as behavioral syndromes, have the potential to inhibit behavioral traits from evolving independently. Limits on behavioral variation can influence a population’s ability to invade new geographic areas and exploit new niches. One way to explore such variation is to examine behavioral syndromes in genetically distinct populations of wide-ranging species that exhibit different dispersal pathways and distances. The personality-dependent dispersal syndrome suggests that certain traits, such as exploratory and risk-taking behavior, are correlated with dispersal success. The Eastern Red-backed Salamander (Plethodon …

“Albertania” And “Egbenema”, Panding Biodiversity In The Oculatellaceae (Cyanobacteria)., Mildred Akagha

Masters Theses

To conduct molecular studies of cyanobacteria of Lagos, Nigeria, a total of eight soil/subaerial samples were collected in Lagos State, Nigeria, and used to isolate cyanobacterial cultures that were characterized microscopically, sequenced, and phylogenetically analyzed. Within the resulting set of cultures, a number of Synechococcales were observed, particularly belonging to Oculatellaceae. “Egbenema” and two new putative species of the recently described genus Albertania were found. Both genera belong to a supported clade within the Oculatellaceae that includes Trichotorquatus and Komarkovaea. The two new putative species of Albertania, “A. egbensis” and “A. latericola”, were from …

One Shell Of A Problem: Cumulative Threat Analysis Of Male Sea Turtles Indicates High Anthropogenic Threat For Migratory Individuals And Gulf Of Mexico Residents, Micah Ashford

Masters Theses

Human use of oceans has dramatically increased in the 21st century, with some of the highest rates of change found within the Gulf of Mexico and Caribbean. Sea turtles are particularly vulnerable to anthropogenic stressors in the marine environment as they make lengthy migrations between foraging and breeding sites often along coastal migration corridors. Sea turtles face severe population pressure from humans, yet little is known about how movement and threats interact specifically for male sea turtles. To better understand male sea turtle movement, and the threats they encounter within their expansive ranges, we tagged 40, adult male sea turtles …

Variation In Alkaloid Composition And Sequestration In Dendrobatid Poison Frogs, Katherine R. Waters

Masters Theses

No abstract provided.

Validation Of Edna Metabarcoding : A Comparison To Traditional Survey Methods In Ozark Streams, Veronica Marian Lee

Masters Theses

Environmental DNA (eDNA) sampling provides a method for assessing fish communities that has potential as a supplement to traditional sampling methods due to its ability to save time as well as its non-invasive nature. This is a method in which from just one sample, eDNA from multiple individual species are able to be sequenced in tandem and the resulting reads identified to describe a community. In order to evaluate this technique and its efficacy for monitoring fish community diversity, we collected water samples alongside surveys performed by the Missouri Department of Conservation sampling program in summers 2020-21. DNA were extracted …