Biology Commons^™

Impacts Of Shoreline Restoration And Source Of Nutrient Enrichment On Macrophytes And Epiphytic Algal Communities, Paige Marie Kleindl

Masters Theses

Macrophytes and their epiphytic algal communities are integral for optimizing littoral ecosystem functioning in lakes. Epiphytic algae’s placement on the plant’s surface can reduce light and nutrient availability (i.e., nitrogen and phosphorus) for the host macrophyte. Macrophyte and epiphytic algal proximity complicates these primary producer group interactions and responses to bioavailable nutrients in the water column or porewater. For example, epiphytic algae may have a competitive advantage over surface water nutrients compared to macrophytes, which may have a competitive advantage over porewater nutrients via root systems.

Muskegon Lake’s industrial history and designation as an Area of Concern prompted shoreline restoration, …

The Implementation Of Qpcr Beach Monitoring Methods: Analysis Of A Multi-Lab Validation Study And The Role Of Environmental Parameters On A Comparison Of Colilert And Qpcr Methods, Molly J. Lane

Masters Theses

Public beaches are routinely tested for potentially pathogenic bacteria to protect beachgoers from possible illness. An EPA approved method, Colilert™, used for testing E. coli in recreational water requires 18 – 22 hours before a result is reported but, recreators have already contacted unsafe water before the beach is closed. My study focused on a U.S. EPA proposed qPCR method (Draft Method C) to quantify E. coli in recreational waters that can provide same-day results. In Chapter 2, I examined the calibration procedure used to validate Draft Method C and compared standard curve intercept and slope estimates calculated with a …

Habitat Use Of The Climate-Sensitive Snowshoe Hare (Lepus Americanus) In The Manistee National Forest In Michigan’S Lower Peninsula, Spencer D. West

Masters Theses

Snowshoe hares (Lepus americanus) are a wide-ranging lagomorph that are important forest herbivores and a popular game species throughout their range. Across the southern boundary of their geographic range, snowshoe hares are experiencing population declines and possible extirpation due to increased predation pressure driven by climate change induced camouflage mismatch, competition for forage, degraded and fragmented habitat. One method of reversing the negative trends in snowshoe hare distribution is to increase and improve available hare habitat. A specific habitat analysis for local regions will most effectively advise managers how to target habitat management. I radio-collared 11 snowshoe hares in the …

Pcr Analysis On The Effects Of Alpha Synuclein Treatment On C6 Rat Glioma Cells, Michael John Helmus

Masters Theses

Multiple System Atrophy (MSA) is a rare neurological disease that mainly implicates the neuronal protein, alpha synuclein, as being centrally involved in MSA pathology. Despite synucleinopathy dogma, little is known about alpha synucleins role in MSA pathogenesis. The Ide laboratory previously conducted an Affymetrix gene expression study utilizing the post-mortem pons tissue from eight individuals with MSA and five individuals without any known neurological disorders. One conclusion from that study was that downregulation of numerous mitochondrial homeostasis related genes had occurred. It is hypothesized that alpha synuclein can perturb mitochondrial homeostasis through direct interaction with mitochondria. This study utilized Reverse …

Characterizing The Interaction Between Non-Pathogenic Fusarium Oxysporum And Arabidopsis Thaliana To Determine Beneficial Effects Conferred To The Model Plant Host, Kathryn Isabelle Vescio

Masters Theses

Fusarium oxysporum (Fo) is a soil-borne fungal pathogen that causes vascular wilt disease on a broad range of plants, including agricultural crops and the model plant Arabidopsis thaliana. There are non-pathogenic members of the Fo species complex that confer defense benefits against other pathogens to the host plant, however alteration to the host’s physiology through interaction with one of these strains, Fo47, have not been described. In this study, we aimed to establish the Fo47-A. thaliana interaction and determine if Fo47 reduces disease severity of a pathogenic Fo isolate, Fo5176. Additionally, we sought to use bioinformatics to mine …

Dynamic Carbon Cycling In Muskegon Lake – A Great Lakes Estuary, Katie Lynn Knapp

Masters Theses

Ecosystem metabolism is the coupling of carbon and oxygen through photosynthesis and respiration. Gross primary production (GPP) is the carbon fixation by photosynthesis, ecosystem respiration (R) is carbon remineralization by bacterial and plankton respiration, and net ecosystem production (NEP) is the balance. Metabolism estimates determine if ecosystem is a sink or source of carbon to the atmosphere. When a lake has a positive NEP, or the GPP:R ratio is greater than 1, it is considered autotrophic and less carbon is being lost to the atmosphere than taken in, whereas if NEP is negative (GPP:R-1d^-1, respectively and the BOD …

Biological Invasions On A Large Scale: Investigating The Spread Of Baby’S Breath (Gypsophila Paniculata) Across North America, Sarah K. Lamar

Masters Theses

Invasive species are a serious threat to biodiversity worldwide. While the impacts of invasive species increase annually, many gaps in our understanding of how these species invade, adapt, and thrive in the novel ecosystems into which they are introduced remain. This thesis aimed to add to our knowledge of invasion science, using the perennial forb Gypsophila paniculata as a study system. Gypsophila paniculata is a shrub native to the Eurasian steppe that was introduced into North America in the late 1800’s. After introduction, G. paniculata quickly spread and now occupies diverse ecosystems across N. America. In chapter II of this …

Habitat Use And Tributary Occupancy Of The Threatened River Redhorse (Moxostoma Carinatum) In The Grand River, Mi, Usa., Nicholas Michael Preville

Masters Theses

The resiliency of our aquatic ecosystems hinges on our ability to protect the native species that reside within them. The river redhorse (Moxostoma carinatum) is one such example and populations have become low enough to warrant listing by the State of Michigan. Causes of decline include overfishing, habitat alteration, and lack of knowledge of basic life-history attributes including their use of non-spawning habitat and spawning locations. In order to understand the river redhorse’s habitat use we implanted 15 individuals with radio transmitters and tracked their locations over the course of a summer. Tagged river redhorse were found to …

An Assessment Of Periphyton Communities In Five Upper Peninsula Streams, Mi, Aaron Jeffrey Christiansen

Masters Theses

This project quantified lotic periphyton community change from May 2018-October 2018 in five, first and second-order Lake Superior tributary streams. Using periphyton communities, land use, geology, and abiotic factors pertinent to stream ecosystems we evaluated periphyton community succession. Using periphytometers, periphyton communities were collected and identified monthly to quantify community succession. Total phosphorus and total Kjeldahl nitrogen were measured monthly during the study. Depth, velocity, specific conductivity, and canopy cover were measured to quantify some of the physical factors within the streams. Nonmetric multidimensional scaling analysis indicated that the periphyton communities were similar between streams (ADONIS p-value =0.73) but was …

Phylogenetic Analysis Of Palisota (Commelinaceae) Using Chloroplast And Nuclear Regions, Alexandra H. Crum

Masters Theses

Palisota (Commelinaceae) differs from other Commelinaceae genera in androecial and pollen characters, a fleshy berry-type fruit, and anatomical characters. Palisota has been divided into two sections based on uniseriate vs. biseriate seed arrangement. Molecular phylogenetic analyses in Commelinaceae have placed Palisota near the base within the family, although its precise position is unclear. We sequenced chloroplast (matK, rbcL, rps16, and trnL-trnF, psbI-psbK, atpB-rbcL, atpF-atpH and psbA-trnH intergenic spacers) and nuclear (AT103) regions in 15 of approximately 26 species of Palisota and 15 outgroup species. Phylogenetic analyses were performed using maximum likelihood and Bayesian methods, with the …

Using Landscape Genetics To Understand Connectivity Of An Island Population Of Eastern Massasauga Rattlesnakes (Sistrurus Catenatus), Nathan Kudla

Masters Theses

Populations that experience low levels of gene flow commonly display increased levels of inbreeding, lower genetic diversity, and reduced adaptive potential. Landscape genetics allows for spatial and genetic information to be analyzed simultaneously to better understand how the landscape influences gene flow. The eastern massasauga rattlesnake (Sistrurus catenatus) is a federally threatened viper found in wetlands throughout the Great Lakes region. Many remaining populations are small and isolated due primarily to habitat loss. Atypical from a range-wide perspective, eastern massasaugas on Bois Blanc Island (BBI), Michigan live in a relatively undisturbed landscape with a potential for high connectivity …

The Association Between Dietary Niche Variation In Rodents And Climate Change Across The Paleocene-Eocene Thermal Maximum, Hannah K. Vermeer

Masters Theses

Mammalian teeth play a crucial role in food acquisition and breakdown and are therefore closely tied to dietary niche. This study reconstructed the diet of early Paleogene paramyid rodents across the Paleocene-Eocene Thermal Maximum (PETM) climatic event in an effort to understand the role of climate in mammalian dietary niche change. Dietary niches were quantified using three dental topographic measures: Dirichlet normal energy, relief index, and orientation patch count rotated. A Kruskal-Wallis test was conducted over eight time periods to determine if each of the dental topographic measures (i.e., diet) varied over time. Regression analysis of these measures with climatic …

Evaluating Remote Site Incubators To Support Restoration Of Arctic Grayling In Michigan, Alan J. Mock

Masters Theses

Remote site incubators (RSIs) have been used to rear salmonid fish eggs along streams in the Pacific Northwest since the 1980s. Recently, the successful use of RSIs for Arctic Grayling Thymallus arcticus restoration in Montana has sparked a renewed interest to reestablish the species in Michigan. To support future reintroduction efforts of Arctic Grayling in Michigan, I evaluated RSIs in three Michigan streams during 2018 and 2019 using Rainbow Trout Oncorhynchus mykiss eggs (as surrogates for Arctic Grayling). My objectives were to: (1) compare hatching success between two different RSI designs (19-L vs. 265-L RSIs), and (2) test whether the …

Thermal Quality Explains Shift In Habitat Association From Forest To Clearings For Terrestrial-Breeding Frogs Along An Elevation Gradient In Colombia, Zachary Lange

Masters Theses

Tropical ectotherms are considered particularly sensitive to changes in the thermal environment from climate change and habitat alteration. Understanding how such species’ thermal physiology relates to their habitat associations in thermally heterogeneous landscapes may help us predict responses and develop sound conservation strategies for the future. We conducted a mark-recapture study of three terrestrial breeding anuran species (Pristimantis medemi, P. savagei, P. frater) in adjacent forest and anthropogenic clearings at field sites spread across seven elevations (415-1350 m asl) in the Colombian Andes. We also performed thermal preference and critical thermal maximum assays in the lab to investigate the relationship …

Astrogliosis In The Adult Zebrafish Olfactory Bulb During Repetitive Peripheral Damage, Jackson Scheib

Masters Theses

Traumatic brain injuries (TBIs), particularly if repetitive, cause massive disruptions of brain homeostasis, significant loss of neurons, and may result in death. Astrocytes are a type of cell that maintain brain homeostasis and neuronal health. Astrogliosis is a continuum of morphological and functional changes of astrocytes in response to damage. This can be neuroprotective or neurotoxic depending on the severity and type of injury. Global astrocyte scarring is typical of neurotoxic astrogliosis and is the source of secondary injury after TBIs. The adult zebrafish olfactory system is known for its capacity to recover from trauma, however, astrocytes in this system …

Visual Attention In Jumping Spiders, Margaret Bruce

Masters Theses

The different ways that animals extract and analyze visual information from their environment is of interest to sensory ecologists. Jumping spiders, well-known for visually guided mating and hunting behavior, are an interesting model for the study of visual attention because they quickly and efficiently integrate information from eight eyes with a small brain. Stimuli in front of the spider are examined by two functionally and morphologically distinct pairs of forward-facing eyes. The principal eyes discern fine details and have small retinas and thus a small visual field. However, their position at the back of moveable tubes within the cephalothorax expands …

The Correlation Between Sleep And Lifespan In Drosophila Melanogaster, Joshua Randall Lisse

Masters Theses

”Adequate sleep is associated with an individual’s health. Too little sleep is associated with many health problems, including cardiovascular disease, obesity, and a general increase in all-cause mortality. Yet the molecular changes that link poor sleep and changes in health are still not well understood. Individuals have a unique daily need for sleep, and deviations from the animal’s regular sleeping patterns can be indicative of, or result in, underlying changes in its health. Therefore, we hypothesize that changes in the sleep architecture in Drosophila melanogaster reflect changes in the fly’s health.

We determined sleep architecture in wild-type male flies over …

Characterization Of Chromosomal Translocations In A Group Of Killifish Species By Using Genome-Wide High-Density Snp Mapping Approach, Naznin Sultana Remex

Masters Theses

"The role of chromosomal rearrangements in reproductive isolation and introgression between species is poorly understood. In heterozygous form, rearrangements may directly interrupt meiotic progression leading to partial sterility/subfertility (underdominance) or may suppress local meiotic segregation (recombination suppression). Such unbalanced meiotic segregation may also result in reproductive isolation and play roles as a driving force of speciation. The objective of this study was to gain insight into the pattern of chromosomal rearrangements in two closely related killifish species in the genus Fundulus (F. notatus, and F. olivaceus) by constructing genetic linkage maps using high-resolution single nucleotide polymorphism (SNP) …

An Assessment Of Night Time And Seasonal Electrofishing In The Lower Wabash River, Eric Christopher Hine

Masters Theses

Large rivers are highly important systems; being exploited both commercially and recreationally. Because of this usage by humans, close monitoring of the ecology of theses rivers is of the utmost importance. The Long-Term Electrofishing project (LTEF) monitors the fish communities of the Illinois, Mississippi, Wabash, and Ohio rivers using day time, pulsed-DC electrofishing during the late Spring through the early Fall each year. Given that previous studies have noted diel and seasonal changes in catch and composition of fish communities, the addition of night time electrofishing may be beneficial to the overarching goals of the LTEF. This study sought to …