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Articles 121 - 129 of 129
Full-Text Articles in Life Sciences
Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, X Li, J Yuan, K B. Kim, Seung J. Baek
Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, X Li, J Yuan, K B. Kim, Seung J. Baek
Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology
The peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear transcription factor that controls the genes involved in metabolism and carcinogenesis. In the present study, we examined the alteration of gene expression in HCT-116 human colorectal cancer cells by PPARgamma agonists: MCC-555 (5 microM), rosiglitazone (5 microM), and 15-deoxy-Delta12,14-prostaglandin J2 (1 microM). The long-oligo microarray data revealed a list of target genes commonly induced (307 genes) and repressed (32 genes) by tested PPARgamma agonists. These genes were analyzed by Onto-Express software and KEGG pathway analysis and revealed that PPARgamma agonists are involved in cell proliferation, focal adhesion, and several signaling pathways. …
Third Thursday 4-2008, Institute Of Agriculture
Pb1775 Common Commercial Pre-Packaged Herbicide Mixtures, The University Of Tennessee Agricultural Extension Service
Pb1775 Common Commercial Pre-Packaged Herbicide Mixtures, The University Of Tennessee Agricultural Extension Service
Field & Commercial Crops
No abstract provided.
Third Thursday 3-2008, Institute Of Agriculture
Third Thursday 2-2008, Institute Of Agriculture
Sp341-A-Protect Farm-Stored Grain, The University Of Tennessee Agricultural Extension Service
Sp341-A-Protect Farm-Stored Grain, The University Of Tennessee Agricultural Extension Service
Field & Commercial Crops
Several insect species attack stored grain, resulting in both direct and indirect losses. Direct losses from insect feeding result in reduced grain weight, loss of nutrients and decreased germination. Indirectly, the presence of insects causes a deterioration and contamination of grain. This results in a poorer grade of grain and lower market value.
The Microscope (2008-2009), Department Of Microbiology
The Microscope (2008-2009), Department Of Microbiology
The Microscope
No abstract provided.
Mutational Optimization Of The Coelenterazine-Dependent Luciferase From Renilla, Jongchan Woo, Albrecht G. Von Arnim
Mutational Optimization Of The Coelenterazine-Dependent Luciferase From Renilla, Jongchan Woo, Albrecht G. Von Arnim
Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology
Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelenterazine substrate. Mutagenesis of active site residue Pro220 extended the half-life of photon emission, yielding brighter luminescence in E. coli. Random mutagenesis uncovered two new mutations that stabilized and increased photon emission in vivo and in vitro, while ameliorating substrate inhibition. Further amended with a previously identified mutation, a new triple mutant showed a threefold improved k …
Third Thursday 1-2008, Institute Of Agriculture