Life Sciences Commons^™

Sp618-2008-2008 Corn Hybrid And Sweet Sorghum Silage Tests In Tennessee, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

No abstract provided.

Rr09-03-2008 Soybean Variety Performance Tests In Tennessee, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

No abstract provided.

W209 Cutleaf Evening Primrose (Oenothera Laciniata Hill), The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

No abstract provided.

The Effects Of Lactation And Negative Energy Balance On Kisspeptin-Stimulated Luteinizing Hormone And Growth Hormone In Dairy Cows, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Herris S. Maxwell, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

Kisspeptin, a neuroendocrine regulator of gonadotropin releasing hormone, is hypothesized to integrate nutrition and hormones critical to metabolism and the regulation of reproduction. Since the negative energy balance of early lactation is associated with reduced fertility via suppression of gonadotropin secretion and enhanced growth hormone (GH) responsiveness, this experiment was designed to determine the effects of stage of lactation and negative energy balance on kisspeptin-10 (Kp-10) stimulated luteinizing hormone (LH) and GH concentrations. Five nonlactating [5.1 ± 0.8 (SEM) years; 577 ± 19 kg body weight (BW)] and five lactating [4.1 ± 0.6 years; 608 ± 11 kg BW] multiparous …

Pb1679 Forest - A - Syst: Self-Assessment To Prioritize Your Forest Uses, The University Of Tennessee Agricultural Extension Service

Forestry, Trees, and Timber

You are unique. The title “Forest Landowner” is one not held by many Tennesseans. Your ownership carries with it a sense of treasure, of enjoyment, of wildness. But it’s also a privilege, one that imparts responsibility, one that can be observed and one that increasingly can be measured.

If you were to be judged on your forestry practices, how would you score? Would you even know which parameters to assess or what questions to ask? Which of these forest uses aesthetics, recreation, soil, timber, water, wildlife—are more important? Have you ever intelligently, and intentionally, began a planning process for your …

Third Thursday 11-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Effects Of Dairy Intake On Weight Maintenance, Michael B. Zemel, Joseph E. Donnelly, Bryan K. Smith, Debra K. Sullivan, Joanna Richards, Danielle Morgan-Hanusa, Matthew S. Mayo, Xiaocun Sun, Galen Cook-Wiens, Bruce W. Bailey, Emily L. Van Walleghen, Richard A. Washburn

Nutrition Publications and Other Works

Background

To compare the effects of low versus recommended levels of dairy intake on weight maintenance and body composition subsequent to weight loss.

Design and Methods

Two site (University of Kansas-KU; University of Tennessee-UT), 9 month, randomized trial. Weight loss was baseline to 3 months, weight maintenance was 4 to 9 months. Participants were maintained randomly assigned to low dairy (< 1 dairy serving/d) or recommended dairy (> 3 servings/d) diets for the maintenance phase. Three hundred thirty eight men and women, age: 40.3 ± 7.0 years and BMI: 34.5 ± 3.1, were randomized; Change in weight and body composition (total fat, trunk fat) from 4 to …

1 Plant Sciences Newsletter Fall 2008, Department Of Plant Sciences

Plant Sciences Newsletter

No abstract provided.

Third Thursday 10-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Mutational Optimization Of The Coelenterazine-Dependent Luciferase From Renilla, Jongchan Woo, Albrecht G. Von Arnim

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelenterazine substrate. Mutagenesis of active site residue Pro220 extended the half-life of photon emission, yielding brighter luminescence in E. coli. Random mutagenesis uncovered two new mutations that stabilized and increased photon emission in vivo and in vitro, while ameliorating substrate inhibition. Further amended with a previously identified mutation, a new triple mutant showed a threefold improved k …

W199 Tennessee Master Gardener Volunteer Handbook, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

The Tennessee Master Gardener Program is a University of Tennessee Extension volunteer program that provides the public with quality home horticulture and gardener-related information through educational programs, examples and demonstrations. Master Gardener volunteers are a diverse group of people with extraordinary talents and abilities which hosts educational and community service projects across the state of Tennessee and the United States. Volunteers are trained with 40 hours of horticultural classes and, in return, volunteers give their community 40 hours of volunteer service through their local UT Extension Office. Master Gardeners are required to maintain a connection to their local UT Extension …

W192-Why Will My Weed Problem Not Go Away? 8 Common Reasons Why Herbicides Fail, The University Of Tennessee Agricultural Extension Service

Home Garden, Lawn, and Landscape

The frustration can be unsettling. ”Why won’t this weed die? I sprayed it with an herbicide, but for the life of me I can not figure out why it’s still here.” This is a very common story we hear from many people trying to control tough weeds in lawns, vegetable gardens, flower beds, nurseries, forests and agricultural fields. Many times just a small change in your approach to controlling the weed is all that is needed, but sometimes the problem can be a bit more complex. Whatever the weed control challenge, it is likely someone else has shared this same …

Pb1621-Best Management Practices For Planting Ornamental Plants, The University Of Tennessee Agricultural Extension Service

Home Garden, Lawn, and Landscape

While spring is a popular planting season, fall may be the best time to plant. Fall planting can help woody ornamental plants survive in the landscape. In fall, plant shoots need fewer nutrients because winter dormancy is approaching. In fall too, carbohydrate plant “food” is produced in leaves and moves to roots, which helps plant growth and survival. Importantly, roots continue to grow until soil temperatures drop below 45-50 F (7-10 C). It is extremely important to water when planting in fall because October and November are usually very dry months with little rainfall. But fallplanted plants also will not …

Pb1621-S-Prácticas Gerenciales Óptimas Para La Plantación De Plantas Ornamentales, The University Of Tennessee Agricultural Extension Service

Home Garden, Lawn, and Landscape

Aunque la primavera es una época popular para plantar, el otoño puede ser la mejor época para hacerlo. La plantación en otoño puede ayudar a que las plantas ornamentales leñosas sobrevivan en el jardín. En otoño, los brotes necesitan menos nutrientes porque se está acercando el estado latente del invierno. También, en otoño, los carbohidratos que sirven como “comida” para la planta, son transportados de las hojas, donde fueron producidos, a las raíces. Esto ayuda al crecimiento y supervivencia de la planta. Las raíces continúan creciendo hasta que la temperatura del suelo baje a más de 45°-50° F (de 7°-10° …

Pb1756 The All Season Pocket Guide To Identifying Common Tennessee Trees, The University Of Tennessee Agricultural Extension Service

Forestry, Trees, and Timber

This booklet was prepared by professional foresters to help you identify Tennessee’s most common trees. It is designed to go to the woods with you (where it is needed) by comfortably riding in your back pocket, pack or cruiser’s jacket. Enjoy!

Third Thursday 9-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Genome Sequence Of The Bacteroides Fragilis Phage Atcc 51477-B1, Shawn A. Hawkins, Alice C. Layton, Steven Ripp, Dan Dan Williams, Gary S. Sayler

Biosystems Engineering and Soil Science Publications and Other Works

The genome of a fecal pollution indicator phage, Bacteroides fragilis ATCC 51477-B1, was sequenced and consisted of 44,929 bases with a G+C content of 38.7%. Forty-six putative open reading frames were identified and genes were organized into functional clusters for host specificity, lysis, replication and regulation, and packaging and structural proteins.

Transcriptional Responses Of Arabidopsis Thaliana Plants To As (V) Stress, Jason M. Abercrombie, Matthew D. Halfhill, Priya Ranjan, Murali R. Rao, Arnold M. Saxton, Joshua S. Yuan, Neal Stewart

Plant Sciences Publications and Other Works

Background

Arsenic is toxic to plants and a common environmental pollutant. There is a strong chemical similarity between arsenate [As (V)] and phosphate (Pi). Whole genome oligonucleotide microarrays were employed to investigate the transcriptional responses of Arabidopsis thaliana plants to As (V) stress.

Results

Antioxidant-related genes (i.e. coding for superoxide dismutases and peroxidases) play prominent roles in response to arsenate. The microarray experiment revealed induction of chloroplast Cu/Zn superoxide dismutase (SOD) (at2g28190), Cu/Zn SOD (at1g08830), as well as an SOD copper chaperone (at1g12520). On the other hand, Fe SODs were strongly repressed in response to As (V) stress. Non-parametric rank …

Rr09-01-2008 Wheat, Barley & Oat Variety Performance Tests In Tennessee, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

No abstract provided.

Pb378 Forage & Field Crop Seeding Guide For Tennessee, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

No abstract provided.

Third Thursday 8-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Using A Literature-Based Nmf Model For Discovering Gene Functional Relationships, Elina Tjioe, Michael W. Berry, Ramin Homayouni, Kevin Heinrich

Faculty Publications and Other Works -- General Biology

No abstract provided.

Differential Transferrin Expression In Placentae From Normal And Abnormal Pregnancies: A Pilot Study, Alena Kralova, Marta Svetlikova, Jindrich Madar, Zdena Ulcova-Gallova, Antonin Bukovsky, Jana Peknicova

Faculty Publications and Other Works -- General Biology

Abstract

Background

The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction.

Methods

In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied …

Third Thursday 7-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Pb1033 Controlling The Alfalfa Weevil In Tennessee, The University Of Tennessee Agricultural Extension Service

Field & Commercial Crops

The alfalfa weevil is present in every county in Tennessee where alfalfa is grown. Producers should be prepared to control this extremely destructive insect whenever necessary if they expect to continue producing this valuable hay crop.

Sp290-N The Greenbug In Grain Sorghum, The University Of Tennessee Agricultural Extension

Field & Commercial Crops

Greenbugs are small (1.6 mm long), soft-bodied insects that are oval in shape. Greenbugs usually feed in colonies by sucking sap from leaves. Feeding may cause mottling and discoloration of the plant. Heavily infested leaves turn red or yellow, shrivel and die. Damaging levels of greenbugs can result in the destruction of entire grain sorghum fields.

Third Thursday 6-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Third Thursday 5-2008, Institute Of Agriculture

Third Thursday

No abstract provided.

Hybridization Produces Novelty When The Mapping Of Form To Function Is Many To One, Nicholas F. Parnell, C Darrin Hulsey, J. Todd Streelman

Faculty Publications and Other Works -- General Biology

Background

Evolutionary biologists want to explain the origin of novel features and functions. Two recent but separate lines of research address this question. The first describes one possible outcome of hybridization, called transgressive segregation, where hybrid offspring exhibit trait distributions outside of the parental range. The second considers the explicit mapping of form to function and illustrates manifold paths to similar function (called many to one mapping, MTOM) when the relationship between the two is complex. Under this scenario, functional novelty may be a product of the number of ways to elicit a functional outcome (i.e., the degree of MTOM). …

Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, X Li, J Yuan, K B. Kim, Seung J. Baek

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

The peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear transcription factor that controls the genes involved in metabolism and carcinogenesis. In the present study, we examined the alteration of gene expression in HCT-116 human colorectal cancer cells by PPARgamma agonists: MCC-555 (5 microM), rosiglitazone (5 microM), and 15-deoxy-Delta12,14-prostaglandin J2 (1 microM). The long-oligo microarray data revealed a list of target genes commonly induced (307 genes) and repressed (32 genes) by tested PPARgamma agonists. These genes were analyzed by Onto-Express software and KEGG pathway analysis and revealed that PPARgamma agonists are involved in cell proliferation, focal adhesion, and several signaling pathways. …