Life Sciences Commons^™

Monitoring Of Levels And Tracking Of Listeria Monocytogenes Strains In A Seafood Processing Environment Using Enrichment Mpn And Rapd, Lynne Mclandsborough, C. Cronin, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmed, R.E. Levin

M. Kathleen Clark

No abstract provided.

Monitoring Of Microflora Present Within A Seafood Processing Environment Using Plating Methodology And Denaturing Gradient Gel Electrophoresis, Lynne Mclandsborough, M. Avallone, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmad, R.E. Levin

M. Kathleen Clark

No abstract provided.

082313333253 Alamat Apotik Jual Obat Tidur / Bius Di Denpasar, Toko Afeng Obat Kuat Bogor

toko afeng obat kuat bogor

Epilepsy-Associated Dysfunction In The Voltage-Gated Neuronal Sodium Channel Scn1a, Christoph Lossin, T. Rhodes, R. Desai, C. Vanoye, S. Caniciu, O. Devinsky, A. George

Christoph Lossin, Ph.D.

Mutations in SCN1A, the gene encoding the brain voltage-gated sodium channel subunit (Nav1.1) are associated with at least two forms of epilepsy, generalized epilepsy with febrile seizures plus (GEFS+) and severe myoclonic epilepsy of infancy (SMEI). We examined the functional properties of four GEFS+ alleles and one SMEI allele using whole-cell patch-clamp analysis of heterologously expressed recombinant human SCN1A. One previously reported GEFS+ mutation (I1656M) and an additional novel allele (R1657C), both affecting residues in a voltage-sensing S4 segment, exhibited a similar depolarizing shift in the voltage dependence of activation. Additionally, R1657C showed a 50% reduction in current density and …

Study On The Wistar Rat Liver And Its Walker Tumor Chromatins By Fluorescence Lifetime And Calorimetric Measurements, Jianfei Zhao

Jianfei Zhao

Morphology Of The Midgut Trunk In The Penaeid Shrimp, Sicyonia Ingentis, Highlighting Novel Nuclear Pore Particles And Fixed Hemocytes., Gary G. Martin, Alice Chiu

Gary Martin

The morphology of the midgut trunk (MGT) in the penaeid shrimp Sicyonia ingentis was examined by light and scanning and transmission electron microscopy. Although the function of the MGT is poorly understood, it is not involved with the digestion and absorption of nutrients, and it appears to be the surface of a shrimp least protected from penetration by potential pathogens. As described for other decapod crustaceans, the MGT in shrimp is composed of a simple columnar epithelium separated from a layer of connective tissue by a thick basal lamina. Beneath the basal lamina is a previously unreported layer of hemocytes, …

The Phylogeny Of Paralabrax (Perciformes: Serranidae) And Allied Taxa Inferred From Partial 16s And 12s Mitochondrial Ribosomal Dna Sequences, Daniel Pondella, Matthew Craig, Jens Frank

Daniel Pondella

Partial sequences of 16S and 12S mitochondrial ribosomal DNA were used to examine the phylogenetic relationships of the primarily eastern Pacific genus Paralabrax (Perciformes: Serranidae) and allied taxa. Paralabrax is considered a basal serranine, which is itself considered the basal subfamily in the Serranidae. Multiple serranines reported closely related to Paralabrax from the genera Serranus, Hypoplectrus, Cratinus, and Centropristis were used as outgroups. Species from the remaining two subfamilies, Epinephilinae and Anthiinae, of the Serranidae were also used in the analyses. The tree of the Serranidae was rooted with the families Polyprionidae and Priacanthidae. Paralabrax, the Serranidae, and the Serraninae …

Cosmopolitan Cyanobacteria, Paula Furey

Paula Furey

No abstract provided.

Is 'The Blues' Black Enough?, Stephen Asma

Stephen T Asma

Reviews the television program "The Blues."

Atp-Independent Contractile Proteins From Plants, Michael Knoblauch, Gundula Noll, Torsten Müller, Dirk Prüfer, Ingrid Schneider-Hüther, Dörte Scharner, Aart Van Bel, Winfried Peters

Winfried S. Peters

N-Acetyl-D-Glucosamine In Crustacean Hemocytes; Possible Functions And Usefulness In Hemocyte Classification., Gary G. Martin, Catherine Castro, Nicholas Moy, Nicole Rubin

Gary Martin

The lectin wheat-germ agglutinin (WGA) selectively binds N-acetyl-D-glucosamine. Fluorescence and electron microscopy were used to show that WGA stains the cytoplasmic granules in the granulocytes, but not the hyaline cells, of two decapods, the ridgeback prawn Sicyonia ingentis and the American lobster Homarus americanus. Using fluorescence microscopy, two intermediate stages in granulocyte maturation were observed. Cells smaller than typical small-granule hemocytes were observed with 5 or fewer granules, which in previous studies using brightfield and phase optics were probably counted as hyaline cells. Also, some granulocytes were observed containing both small and large granules, supporting the suggestion that small and …

Metabolic Responses Of Shorebird Chicks To Cold Stress: Hysteresis Of Cooling And Warming Phases, Robert E. Ricklefs, Joseph B. Williams

Robert Ricklefs

Maturation Of Cardiovascular Control Mechanisms In The Embryonic Emu (Dromiceius Novaehollandiae), Dane Crossley, Brian Bagatto, Edward Dzialowski, Warren Burggren

Brian P. Bagatto

Our understanding of avian embryonic cardiovascular regulation has been based on studies in chickens. The present study was undertaken to determine if the patterns established in chickens are generally applicable to the emu, a ratite bird species. We studied cardiovascular physiology over the interval from 60% to 90% of the emu's 50-day incubation period. During this period, embryonic emus exhibit a slight fall in resting heart rate (from 171 beats min(-1) to 154 beats min(-1)) and a doubling of mean arterial pressure (from 1.2 kPa to 2.6 kPa). Exposures to 15% or 10% O(2) initially decreased heart rate during the …

Electromyographic Analysis Of Abdominal Muscle Activity Using Portable Abdominal Exercise Devices And A Traditional Crunch, Stuart Rugg

Stuart Rugg

The purpose of this study was to compare the abdominal muscle activity elicited while using 4 portable abdominal training devices vs. a traditional crunch. Thirty-three adults participated in this study. The exercise devices tested included the Ab Roller Plus, Torso Track 2, AB-DOer Pro, and the Perfect Abs. All subjects were tested on the Perfect Abs in both a seated and supine position using low-, medium-, and high-resistance bands. The Torso Track 2 was also tested at low- and high-resistance settings. Surface electromyography (EMG) was recorded from the upper and lower portions of the rectus abdominis, external oblique, and the …

Plasmepsin 4, The Food Vacuole Aspartic Proteinase Found In All Plasmodium Spp. Infecting Man, John B. Dame, Charles A. Yowell, Levi Omara-Opyene, Jane M. Carlton, Roland A. Cooper, Tang Li

Roland A. Cooper

Role Of Micrornas In Plant And Animal Development, Victor Ambros, James Carrington

Victor R. Ambros

Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. They are involved in many molecular interactions, including defense against viruses and regulation of gene expression during development. miRNAs interfere with expression of messenger RNAs encoding factors that control developmental timing, stem cell maintenance, and other developmental and physiological processes in plants and animals. miRNAs are negative regulators that function as specificity determinants, or guides, within complexes that inhibit protein synthesis (animals) or promote degradation (plants) of mRNA targets.

Microrna Pathways In Flies And Worms: Growth, Death, Fat, Stress, And Timing, Victor Ambros

Victor R. Ambros

Drosophila geneticists have uncovered roles for microRNAs in the coordination of cell proliferation and cell death during development, and in stress resistance and fat metabolism. In C. elegans, a homolog of the well-known fly developmental regulator hunchback acts downstream of the microRNAs lin-4 and let-7 in a pathway controlling developmental timing.

Endogenous Oxidoreductase Expression Is Induced By Aminoglycosides, George Hoppe, Yuh-Cherng Chai, Jonathan Sears

Yuh-Cherng Chai

Oxidoreductases such as glutaredoxin are a major class of enzymes that reversibly catalyze thiol-disulfide exchange reactions. Transfection experiments using geneticin (G418) selection to identify the specific protein S-thiolated substrates of glutaredoxin-1 (Grx-1) noted the curious phenomenon that nontransfected control cells treated with G418 had increased levels of Grx-1 expression. Varied concentrations of gentamicin, kanamycin, and hygromycin increased Grx-1 expression in a time- and dose-dependent fashion in human cultured retinal pigment epithelial cells. Reactive oxygen species formation after aminoglycoside exposure correlated directly to aminoglycoside treatment. Further indication that oxidation regulates Grx-1 expression was noted by the positive effect of phorbol 12-myristate …

A Model For Shear Stress Sensing And Transmission In Vascular Endothelial Cells, Borbala Mazzag, John S. Tamaresis, Abdul Barakat

Borbala Mazzag

Micrornas And Other Tiny Endogenous Rnas In C. Elegans, Victor Ambros, Rosalind Lee, Ann Lavanway, Peter Williams, David Jewell

Victor R. Ambros

BACKGROUND: MicroRNAs (miRNAs) are small noncoding RNAs that are processed from hairpin precursor transcripts by Dicer. miRNAs probably inhibit translation of mRNAs via imprecise antisense base-pairing. Small interfering RNAs (siRNAs) are similar in size to miRNAs, but they recognize targets by precise complementarity and elicit RNA-mediated interference (RNAi). We employed cDNA sequencing and comparative genomics to identify additional C. elegans small RNAs with properties similar to miRNAs and siRNAs. RESULTS: We found three broad classes of small RNAs in C. elegans: (1) 21 new miRNA genes (we estimate that C. elegans contains approximately 100 distinct miRNA genes, about 30% of …

The Inter Allied Games Of 1919, Don Morrow

Donald Morrow

No abstract provided.

Amplied Birdstrike Risk Related To Population Increases Of Large Birds In North America, Paul F. Eschenfelder, Richard Dolbeer

Paul F. Eschenfelder

No abstract provided.

Effect Of Fermentation On The Quality Characteristics Of Nixtamalized Corn, Emmanuel Ohene Afoakwa, Samuel Sefa-Dedeh, Beatrice Cornelius

Professor Emmanuel Ohene Afoakwa

Spontaneous lactic acid fermentation is an important process in cereal processing. It is applied to develop and enhance taste and flavour, modify texture and improve the microbial safety of foods. When applied to nixtamalized corn mixed with traditional steeped corn it is expected to further improve the functionality, improve nutritional quality and provide an alternative corn-based ingredient. The objective of this study was to investigate the chemical and functional properties of fermented blends of steeped and nixtamalized corn. A 3_5 factorial experimental design with fermentation time (0, 24, 48 h) and blends composition (0:100, 25:75, 50:50, 75:25, 100:0 steeped:nixtamalized corn) …

Lack Of Widespread Organochlorine Pesticide Contamination In South American Resident Passerines, Given Harper, A. P. Capparella, J. A. Klemens, J. A. Frick

Given Harper

Application Of Response Surface Methodology For Studying The Quality Characteristics Of Cowpea-Fortified Nixtamalized Maize, Samuel Sefa-Dedeh, Beatrice Cornelius, Esther Sakyi-Dawson, Emmanuel Ohene Afoakwa

Professor Emmanuel Ohene Afoakwa

Studies were conducted on the chemical and functional properties of fermented cowpea-fortified nixtamalized maize using response surface methodology. The central composite rotatable design for Ks3 was used to study the combined effect of lime concentration (0–1%), moisture content (55–65%) and cowpea level (0–30%) on pH, titratable acidity, water absorption, protein and viscosity of fermented nixtamalized maize. Regression models were developed to predict the variables. The presence of lime and cowpea influenced the pH, titratable acidity, water absorption, protein content and the cooked paste viscosity of the fermented cowpea-fortified maize. The presence of lime during fermentation generally decreased titratable acidity, water …

A Uniform System For Microrna Annotation, Victor R. Ambros, Bonnie Bartel, David P. Bartel, Christopher B. Burge, James C. Carrington, Xuemei Chen, Gideon Dreyfuss, Sean R. Eddy, Sam Griffiths-Jones, Mhairi Marshall, Marjori Matzke, Gary Ruvkun, Thomas Tuschl

Victor R. Ambros

MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for the identification and annotation of new miRNAs from diverse organisms, particularly so that miRNAs can be reliably distinguished from other RNAs such as small interfering RNAs. We describe specific criteria for the experimental verification of miRNAs, and conventions for naming miRNAs and miRNA genes. Finally, an online clearinghouse for miRNA gene name assignments is provided by the Rfam database of RNA families.

Reversal Of Protein S-Glutathiolation By Glutaredoxin In The Retinal Pigment Epithelium., Yuh-Cherng Chai, George Hoppe, Jonathan Sears

Yuh-Cherng Chai

Protein cysteines can serve both sensory and activation roles in the regulation of protein function. The modulation of mixed disulfides with glutathione may promise to be a broad mechanism of redox signalling. Using both protein extract and intact RPE cells, we have generated covalent adduction of glutathione to protein cysteines and further show that glutaredoxin (Grx-1) is able to remove glutathione from protein S-glutathiolated substrates. Our data demonstrate that glutathione can modify a wide range of RPE proteins in intact cells, but that the reversal of this process–deglutathiolation and thiol bond restoration–may require a specific catalytic reaction with glutaredoxin. More …

Sequential Radiation Of Unrelated Organisms: The Gall Fly Eurosta Solidaginis And The Tumbling Flower Beetle Mordellistena Convicta, Warren Abrahamson, C.P. Blair, M.D. Eubanks, S.A. Morehead

Warren G. Abrahamson, II

No abstract provided.

Aquaculture, Michael Rice

Michael A Rice

This is the chapter on small-scale aquaculture within a volume intended as a guide for practitioners engaged in sustainable rural livelihood and food production projects in the developing world. Included in this chapter on aquaculture are fundamentals of aquaculture water quality, selection of sites and construction methods for fish ponds, and management techniques for carp and tilapia which are nutritious and easy to grow in artisanal ponds. The fundamentals of cage culture of fish is also covered, along with the fundamentals of culturing oysters and other molluscan shellfish in coastal waters.

[James Mark] Baldwin And His Many Effects, David Depew

David J Depew

No abstract provided.