Life Sciences Commons^™

Monitoring Of Levels And Tracking Of Listeria Monocytogenes Strains In A Seafood Processing Environment Using Enrichment Mpn And Rapd, Lynne Mclandsborough, C. Cronin, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmed, R.E. Levin

M. Kathleen Clark

No abstract provided.

Monitoring Of Microflora Present Within A Seafood Processing Environment Using Plating Methodology And Denaturing Gradient Gel Electrophoresis, Lynne Mclandsborough, M. Avallone, M. Clark, R. Witkowsky, H. Lui, A. Sayedahmad, R.E. Levin

M. Kathleen Clark

No abstract provided.

Geobacter Sulfurreducens Can Grow With Oxygen As A Terminal Electron Acceptor, W. C. Lin, Maddalena V. Coppi, Derek Lovley

Derek Lovley

Geobacter sulfurreducens, previously classified as a strict anaerobe, tolerated exposure to atmospheric oxygen for at least 24 h and grew with oxygen as the sole electron acceptor at concentrations of 10% or less in the headspace. These results help explain how Geobacter species may survive in oxic subsurface environments, being poised to rapidly take advantage of the development of anoxic conditions.

The Zebrafish Iguana Locus Encodes Dzip1, A Novel Zinc Finger Protein Required For Proper Regulation Of Hedgehog Signaling, Kohshin Sekimizu, Noriyuki Nishioka, Hiroshi Sasaki, Hiroyuki Takeda, Rolf O. Karlstrom, Atsushi Kawakami

Rolf O Karlstrom

Members of the Hedgehog (Hh) family of intercellular signaling molecules play crucial roles in animal development. Aberrant regulation of Hh signaling in humans causes developmental defects, and leads to various genetic disorders and cancers. We have characterized a novel regulator of Hh signaling through the analysis of the zebrafish midline mutant iguana (igu). Mutations in igu lead to reduced expression of Hh target genes in the ventral neural tube, similar to the phenotype seen in zebrafish mutants known to affect Hh signaling. Contradictory at first sight, igu mutations lead to expanded Hh target gene expression in somites. Genetic and pharmacological …

082313333253 Alamat Apotik Jual Obat Tidur / Bius Di Denpasar, Toko Afeng Obat Kuat Bogor

toko afeng obat kuat bogor

Epilepsy-Associated Dysfunction In The Voltage-Gated Neuronal Sodium Channel Scn1a, Christoph Lossin, T. Rhodes, R. Desai, C. Vanoye, S. Caniciu, O. Devinsky, A. George

Christoph Lossin, Ph.D.

Mutations in SCN1A, the gene encoding the brain voltage-gated sodium channel subunit (Nav1.1) are associated with at least two forms of epilepsy, generalized epilepsy with febrile seizures plus (GEFS+) and severe myoclonic epilepsy of infancy (SMEI). We examined the functional properties of four GEFS+ alleles and one SMEI allele using whole-cell patch-clamp analysis of heterologously expressed recombinant human SCN1A. One previously reported GEFS+ mutation (I1656M) and an additional novel allele (R1657C), both affecting residues in a voltage-sensing S4 segment, exhibited a similar depolarizing shift in the voltage dependence of activation. Additionally, R1657C showed a 50% reduction in current density and …

Fisheries In Sundarbans: Problems And Prospects, Ganesh Chandra, R L. Sagar

Ganesh Chandra

Sundarbans, the largest delta on the planet earth is famous for its marine and estuarine fish resources. A large population is dependent on fishery activity and capture fisheries is treated as the backbone of Sundarban economy. Sundarban boast around 172 species of fishes, 20 species of prawn and 44 species of crabs including two edible crabs. But fisheries in Sundarbans faces some difficult problems which have an impact on the biodiversity, sustainability and livelihood of fish resources and fisher folk viz. shrinking tiger prawn population, indiscriminate fish seed collection, lack of post harvest and other infrastructures, natural calamities such as …

Cd43 Modulates Severity And Onset Of Experimental Autoimmune Encephalomyelitis., Thandi M. Onami, M. L. Ford, A. Sperling, R. Ahmed, B. D. Evavold

Thandi M. Onami

Experimental autoimmune encephalomyelitis (EAE) is a mouse model of multiple sclerosis characterized by infiltration of activated CD4(+) T lymphocytes into tissues of the CNS. This study investigated the role of CD43 in the induction and progression of EAE. Results demonstrate that CD43-deficient mice have reduced and delayed clinical and histological disease severity relative to CD43(+/+) mice. This reduction was characterized by decreased CD4(+) T cell infiltration of the CNS of CD43(-/-) mice but similar numbers of Ag-specific T cells in the periphery, suggesting a defect in T cell trafficking to the CNS. The absence of CD43 also affected cytokine production, …

Study On The Wistar Rat Liver And Its Walker Tumor Chromatins By Fluorescence Lifetime And Calorimetric Measurements, Jianfei Zhao

Jianfei Zhao

A Hot-Vent Gastropod With Iron Sulfide Biomineralized Dermal Sclerites, Anders Waren, Stefan Bengtson, Shana K. Goffredi, Cindy L. Van Dover

Shana Goffredi

No abstract provided.

Morphology Of The Midgut Trunk In The Penaeid Shrimp, Sicyonia Ingentis, Highlighting Novel Nuclear Pore Particles And Fixed Hemocytes., Gary G. Martin, Alice Chiu

Gary Martin

The morphology of the midgut trunk (MGT) in the penaeid shrimp Sicyonia ingentis was examined by light and scanning and transmission electron microscopy. Although the function of the MGT is poorly understood, it is not involved with the digestion and absorption of nutrients, and it appears to be the surface of a shrimp least protected from penetration by potential pathogens. As described for other decapod crustaceans, the MGT in shrimp is composed of a simple columnar epithelium separated from a layer of connective tissue by a thick basal lamina. Beneath the basal lamina is a previously unreported layer of hemocytes, …

Electron Transfer By Desulfobulbus Propionicus To Fe(Iii) And Graphite Electrodes, Derek Lovley, Dawn E. Holmes, Daniel R. Bond

Derek Lovley

Desulfobulbus propionicus was able to grow with Fe(III), the humic acids analog anthraquinone-2,6-disulfonate (AQDS), or a graphite electrode as an electron acceptor. These results provide an explanation for the enrichment of Desulfobulbaceae species on the surface of electrodes harvesting electricity from anaerobic marine sediments and further expand the diversity of microorganisms known to have the ability to use both sulfate and Fe(III) as an electron acceptor.

Estimations Of Ammonia And Hydrogen Sulfide Fluxes From Cattle Feedlot Surfaces In Texas High Plains, Bok-Haeng Baek, Jacek A. Koziel, Jarett P. Spinhirne, David B. Parker, Nolan A. Cole

Jacek A. Koziel

A dynamic flow-through chamber system and continuous analyzers were used for on-site measurements of ammonia-nitrogen (NH 3 -N) and hydrogen sulfide-sulfur (H 2 S-S) fluxes from commercial feedlot surfaces in northwestern Texas during two week period in the summer of 2002. Ambient air and air inside chamber temperatures, and manure pack temperature were measured continuously. Manure pack moisture content, pH, and TKN were measured daily to characterize its correlation with NH 3 -N fluxes. The preliminary average NH 3 -N flux from the feedlot surface for the entire measurement period was 1,669 ± 1,212 NH 3 -N µ g/m 2 …

Generation And Calibration Of Standard Gas Mixtures For Volatile Fatty Acids Using Permeation Tubes And Solid Phase Microextraction, Jacek A. Koziel, Jarett P. Spinhirne

Jacek A. Koziel

Volatile fatty acids (VFAs) are major components of odors associated with agricultural operations and livestock housing, solid waste processing and disposal, and industrial and municipal wastewater collection and treatment systems. Emissions estimation and assessment of odor control methods depend on reliable air sampling and analysis methods for VFAs. The objective of this research was to develop and test a method for continuous and reliable generation of standard gas mixtures for VFAs based on permeation tubes. Standard gas mixtures for acetic, propionic, isobutyric, butyric, isovaleric, valeric, and hexanoic acids were generated with permeation tubes and monitored for a 100-day period. The …

Quality Control And Peak Finding For Proteomics Data Collected From Nipple Aspirate Fluid Using Surface Enhanced Laser Desorption And Ionization., Jeffrey S. Morris, Kevin R. Coombes, Herbert A. Fritsche, Charlotte Clarke, Jeng-Neng Chen, Keith A. Baggerly, Lian-Chun Xiao, Mien-Chie Hung, Henry M. Kuerer

Jeffrey S. Morris

Background: Recently, researchers have been using mass spectroscopy to study cancer. For use of proteomics spectra in a clinical setting, stringent quality-control procedures will be needed.

Methods: We pooled samples of nipple aspirate fluid from healthy breasts and breasts with cancer to prepare a control sample. Aliquots of the control sample were used on two spots on each of three IMAC ProteinChip® arrays (Ciphergen Biosystems, Inc.) on 4 successive days to generate 24 SELDI spectra. In 36 subsequent experiments, the control sample was applied to two spots of each ProteinChip array, and the resulting spectra were analyzed to determine how …

Sensitization Of Staphylococcus Aureus And Escherichia Coli To Antibiotics By The Sesquiterpenoids Nerolidol, Farnesol, Bisabolol, And Apritone, Byron F. Brehm-Stecher, Eric A. Johnson

Byron F. Brehm-Stecher

The sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone were investigated for their abilities to enhance bacterial permeability and susceptibility to exogenous antimicrobial compounds. Initially, it was observed by flow cytometry that these sesquiterpenoids promoted the intracellular accumulation of the membrane-impermeant nucleic acid stain ethidium bromide by live cells of Lactobacillus fermentum, suggesting that enhanced permeability resulted from disruption of the cytoplasmic membrane. The ability of these sesquiterpenoids to increase bacterial susceptibility to a number of clinically important antibiotics was then investigated. In disk diffusion assays, treatment with low concentrations (0.5 to 2 mM) of nerolidol, bisabolol, or apritone enhanced the susceptibility …

Does Landscape Diversity Slow The Spread Of Rotation-Resistant Western Corn Rootworm (Coleoptera: Chrysomelidae)?, D. W. Onstad, D. W. Crwoder, S. A. Isard, E. Levine, J. L. Spencer, Matthew E. O'Neal, S. T. Ratcliffe, M. E. Gray, L. W. Bledsoe, C. D. Di Fonzo, J. B. Eisley, C. R. Edwards

Matthew E. O'Neal

A behavioral change in some western corn rootworm (Diabrotica virgifera virgifera LeConte) populations is threatening the effectiveness of crop rotation, a successful management strategy for controlling this pest. We created a set of simple meteorologic and behavioral models that can be used to predict the spread of the beetle infesting soybean (Glycine max (L.)) throughout the midwestern United States. We used data collected in Illinois, IN, MI, and Ohio to create maps of observations to evaluate the model. We displayed data on the maps using detection thresholds for western corn rootworm in soybean fields of 10 or 20 beetles per …

Ammonia Concentrations In Poultry Broiler Production Units Treated With Liquid Alum, K. A. Armstrong, Robert T. Burns, F. R. Walker, L. R. Wilhelm, D. Raj Raman

D. Raj Raman

High ammonia concentrations in commercial broiler production houses can result in poor bird performance, lower feed conversion ratios, and higher mortalities. Growers have traditionally controlled in-house ammonia levels by increasing ventilation. During cold weather, increased ventilation rates result in higher heating energy requirements. Granular alum (aluminum sulfate, Al2(SO4)3 · 14H2O) has been successfully used as a litter amendment to reduce ammonia volatilization from litter inside broiler production houses. In this study, liquid aluminum sulfate was investigated as a litter amendment for ammonia suppression in four commercial poultry-broiler units. This project investigates four treatment levels of liquid alum in four adjacent …

The Phylogeny Of Paralabrax (Perciformes: Serranidae) And Allied Taxa Inferred From Partial 16s And 12s Mitochondrial Ribosomal Dna Sequences, Daniel Pondella, Matthew Craig, Jens Frank

Daniel Pondella

Partial sequences of 16S and 12S mitochondrial ribosomal DNA were used to examine the phylogenetic relationships of the primarily eastern Pacific genus Paralabrax (Perciformes: Serranidae) and allied taxa. Paralabrax is considered a basal serranine, which is itself considered the basal subfamily in the Serranidae. Multiple serranines reported closely related to Paralabrax from the genera Serranus, Hypoplectrus, Cratinus, and Centropristis were used as outgroups. Species from the remaining two subfamilies, Epinephilinae and Anthiinae, of the Serranidae were also used in the analyses. The tree of the Serranidae was rooted with the families Polyprionidae and Priacanthidae. Paralabrax, the Serranidae, and the Serraninae …

Cosmopolitan Cyanobacteria, Paula Furey

Paula Furey

No abstract provided.

Is 'The Blues' Black Enough?, Stephen Asma

Stephen T Asma

Reviews the television program "The Blues."

Atp-Independent Contractile Proteins From Plants, Michael Knoblauch, Gundula Noll, Torsten Müller, Dirk Prüfer, Ingrid Schneider-Hüther, Dörte Scharner, Aart Van Bel, Winfried Peters

Winfried S. Peters

N-Acetyl-D-Glucosamine In Crustacean Hemocytes; Possible Functions And Usefulness In Hemocyte Classification., Gary G. Martin, Catherine Castro, Nicholas Moy, Nicole Rubin

Gary Martin

The lectin wheat-germ agglutinin (WGA) selectively binds N-acetyl-D-glucosamine. Fluorescence and electron microscopy were used to show that WGA stains the cytoplasmic granules in the granulocytes, but not the hyaline cells, of two decapods, the ridgeback prawn Sicyonia ingentis and the American lobster Homarus americanus. Using fluorescence microscopy, two intermediate stages in granulocyte maturation were observed. Cells smaller than typical small-granule hemocytes were observed with 5 or fewer granules, which in previous studies using brightfield and phase optics were probably counted as hyaline cells. Also, some granulocytes were observed containing both small and large granules, supporting the suggestion that small and …

Metabolic Responses Of Shorebird Chicks To Cold Stress: Hysteresis Of Cooling And Warming Phases, Robert E. Ricklefs, Joseph B. Williams

Robert Ricklefs

Genome-Wide Scan For Quantitative Trait Loci Influencing Ldl Size And Plasma Triglyceride In Familial Hypertriglyceridemia, Melissa A. Austin, Karen L. Edwards, Stephanie A. Monks, Kent M. Koprowicz, John D. Brunzell, Arno G. Motulsky, Michael C. Mahaney, James E. Hixson

Kent M Koprowicz

No abstract provided.

Herbs In American Fields: A Horticultural Perspective Of Herb And Medicinal Plant Production In The United States, Lyle E. Craker, Zoë Gardner, Selma C. Etter

Lyle E. Craker

No abstract provided.

Reproductive Physiology Of Free-Living White Ibises (Eudocimus Albus) In The Florida Everglades, Julie A. Heath, Peter C. Frederick, Thea M. Edwards, Louis J. Guillette Jr.

Julie Heath

We measured plasma concentrations of testosterone, estradiol, progesterone, and corticosterone; and recorded changes in gonad size, body condition, molt, and brood patch development of free-living adult White Ibises (Eudocimus albus) during the breeding season in the Florida Everglades. White Ibises are colonially breeding, long-legged wading birds that inhabit freshwater and estuarine wetlands. They have flexible breeding schedules (nest initiation dates can range from January to September) and onset of nesting is usually associated with increased prey availability caused by concentration of small fish in pools during periods of wetland drying. In this paper, we present the hormonal and physical characteristics …

Maturation Of Cardiovascular Control Mechanisms In The Embryonic Emu (Dromiceius Novaehollandiae), Dane Crossley, Brian Bagatto, Edward Dzialowski, Warren Burggren

Brian P. Bagatto

Our understanding of avian embryonic cardiovascular regulation has been based on studies in chickens. The present study was undertaken to determine if the patterns established in chickens are generally applicable to the emu, a ratite bird species. We studied cardiovascular physiology over the interval from 60% to 90% of the emu's 50-day incubation period. During this period, embryonic emus exhibit a slight fall in resting heart rate (from 171 beats min(-1) to 154 beats min(-1)) and a doubling of mean arterial pressure (from 1.2 kPa to 2.6 kPa). Exposures to 15% or 10% O(2) initially decreased heart rate during the …

Electromyographic Analysis Of Abdominal Muscle Activity Using Portable Abdominal Exercise Devices And A Traditional Crunch, Stuart Rugg

Stuart Rugg

The purpose of this study was to compare the abdominal muscle activity elicited while using 4 portable abdominal training devices vs. a traditional crunch. Thirty-three adults participated in this study. The exercise devices tested included the Ab Roller Plus, Torso Track 2, AB-DOer Pro, and the Perfect Abs. All subjects were tested on the Perfect Abs in both a seated and supine position using low-, medium-, and high-resistance bands. The Torso Track 2 was also tested at low- and high-resistance settings. Surface electromyography (EMG) was recorded from the upper and lower portions of the rectus abdominis, external oblique, and the …

Plasmepsin 4, The Food Vacuole Aspartic Proteinase Found In All Plasmodium Spp. Infecting Man, John B. Dame, Charles A. Yowell, Levi Omara-Opyene, Jane M. Carlton, Roland A. Cooper, Tang Li

Roland A. Cooper