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Articles 451 - 478 of 478

Full-Text Articles in Life Sciences

Two Forms Of The Large Tumor Suppressor Gene (Lats1) Protein Expressed In The Vertebrate Retina, Novrouz B. Akhmedov, Clyde K. Yamashita, Dai Tran, Natik I. Piri, Gustavo D. Aguirre, Debora B. Farber Mar 2005

Two Forms Of The Large Tumor Suppressor Gene (Lats1) Protein Expressed In The Vertebrate Retina, Novrouz B. Akhmedov, Clyde K. Yamashita, Dai Tran, Natik I. Piri, Gustavo D. Aguirre, Debora B. Farber

Gustavo D. Aguirre, VMD, PhD

The large tumor suppressor gene (Lats1) encodes a protein kinase that is highly conserved from fly to human, and plays a crucial role in the prevention of tumor formation by controlling mitosis progression. We have found that in addition to the previously isolated 7.5 kb long form of Lats1 (Lats1L) mRNA, a less abundant, shorter, 3.4 kb primary transcript (Lats1S) also is expressed in the vertebrate retina. Compared toLats1L, the sequence of Lats1S mRNA has a deletion of exons 6, 7, and 8 that corresponds to 792 bp …


What Communities Should Do Pre-Event To Support Public Health Post-Event Assessments, Surveillance And Monitoring, Thomas Lyons Carr Iii Feb 2005

What Communities Should Do Pre-Event To Support Public Health Post-Event Assessments, Surveillance And Monitoring, Thomas Lyons Carr Iii

Thomas Lyons (Thom) Carr III Appl.Sc., CEM

[Abstract written March 2008, TLC] Under worst-case planning assumptions used by some major metropolitan areas, a Neighbor-to-Neighbor self-help program model is the primary link between citizens and the professional response personnel of the responsible government agencies.

In the Neighbor-to-Neighbor self-help program model or a Community Emergency Management Plan (CEMP) calls on the citizens in neighborhoods to identify and establish cluster emergency preparedness committees, Cluster Emergency Coordination Centers (CECC) and Community Emergency Response Teams (CERT). Missing from these plans or what is not articulated is how constant Public Health Post-Event Surveillance, Monitoring and Assessments will be done. Given the worst-case planning …


Bioinformatic Analysis Of The Metal-Binding Protein Families And Heavy Metal Resistance Amongst Cyanobacteria, Tin-Chun Chu, Lee Lee, Shankar Srinivasan Dec 2004

Bioinformatic Analysis Of The Metal-Binding Protein Families And Heavy Metal Resistance Amongst Cyanobacteria, Tin-Chun Chu, Lee Lee, Shankar Srinivasan

Tin-Chun Chu, Ph.D.

No abstract provided.


Microbial Diversity In Hot Spring Cyanobacterial Mats: Pattern And Prediction, Frederick M. Cohan, D. M. Ward Dec 2004

Microbial Diversity In Hot Spring Cyanobacterial Mats: Pattern And Prediction, Frederick M. Cohan, D. M. Ward

Frederick M. Cohan

No abstract provided.


Which Species Concept For Bacteria?—An E-Debate, S. Godreuil, Frederick M. Cohan, H. Shah, M. Tibayrenc Dec 2004

Which Species Concept For Bacteria?—An E-Debate, S. Godreuil, Frederick M. Cohan, H. Shah, M. Tibayrenc

Frederick M. Cohan

No abstract provided.


Food Based Approaches For A Healthy Nutrition In Africa, Mamoudou Hama Dicko May 2004

Food Based Approaches For A Healthy Nutrition In Africa, Mamoudou Hama Dicko

Pr. Mamoudou H. DICKO, PhD

The latest estimates of the FAO demonstrate the problems of the fight against hunger. These problems are manifested by the ever-increasing number of chronically undernourished people worldwide. Their numbers during the 1999-2001 period were estimated at about 840 million of which 798 million live in developing countries. Sub-Saharan Africa alone represented 198 million of those. In this part of Africa the prevalence of undernourishment ranges from 5-34%, causing growth retardation and insufficient weight gain among one third of the children under five years of age and resulting in a mortality of 5-15% among these children. Malnutrition resulting from undernourishment is …


Novel Forms Of Structural Integration Between Microbes And A Vent Gastropod From The Indian Ocean, Shana K. Goffredi, Anders Waren, Victoria J. Orphan, Cindy L. Van Dover, Robert C. Vrijenhoek Apr 2004

Novel Forms Of Structural Integration Between Microbes And A Vent Gastropod From The Indian Ocean, Shana K. Goffredi, Anders Waren, Victoria J. Orphan, Cindy L. Van Dover, Robert C. Vrijenhoek

Shana Goffredi

Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of γ-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting differs from that of all other vent mollusks, which harbor sulfur-oxidizing endosymbionts in their gills. The significantly reduced digestive tract, the isotopic signatures of the snail tissues, and the presence of internal bacteria suggest a dependence on chemoautotrophy for nutrition. Most notably, this snail is unique in having a dense coat of mineralized …


The Helicobacter Pylori Flaa1 And Wbpb Genes Control Lipopolysaccharide And Flagellum Synthesis And Function, Alexandra Merkx-Jacques, R. Obhi, G. Bethune, C. Creuzenet Mar 2004

The Helicobacter Pylori Flaa1 And Wbpb Genes Control Lipopolysaccharide And Flagellum Synthesis And Function, Alexandra Merkx-Jacques, R. Obhi, G. Bethune, C. Creuzenet

Alexandra Merkx-Jacques

flaA1 and wbpB are conserved genes with unknown biological function in Helicobacter pylori. Since both genes are predicted to be involved in lipopolysaccharide (LPS) biosynthesis, flagellum assembly, or protein glycosylation, they could play an important role in the pathogenesis of H. pylori. To determine their biological role, both genes were disrupted in strain NCTC 11637. Both mutants exhibited altered LPS, with loss of most O-antigen and core modification, and increased sensitivity to sodium dodecyl sulfate compared to wild-type bacteria. These defects could be complemented in a gene-specific manner. Also, flaA1 could complement these defects in the wbpB mutant, suggesting a …


Ethiopian Soils Harbor Natural Populations Of Rhizobia That Form Symbioses With Common Bean (Phaseolus Vulgaris L.), Serawit Kassa, Desta Beyene, Franklin Ampy, Amha Asseffa, Tadesse Gebremedhin, Peter Van Berkum Jan 2004

Ethiopian Soils Harbor Natural Populations Of Rhizobia That Form Symbioses With Common Bean (Phaseolus Vulgaris L.), Serawit Kassa, Desta Beyene, Franklin Ampy, Amha Asseffa, Tadesse Gebremedhin, Peter Van Berkum

Franklin Ampy

The diversity and taxonomic relationships of 83 bean-nodulating rhizobia indigenous to Ethiopian soils were characterized by PCR-RFLP of the internally transcribed spacer (ITS) region between the 16S and 23S rRNA genes, 16S rRNA gene sequence analysis, multilocus enzyme electrophoresis (MLEE), and amplified fragment-length polymorphism. The isolates fell into 13 distinct genotypes according to PCR-RFLP analysis of the ITS region. Based on MLEE, the majority of these genotypes (70%) was genetically related to the type strain of Rhizobium leguminosarum. However, from analysis of their 16S rRNA genes, the majority was placed with Rhizobium etli. Transfer and recombination of the 16S rRNA …


Concepts Of Bacterial Biodiversity For The Age Of Genomics, Frederick M. Cohan Dec 2003

Concepts Of Bacterial Biodiversity For The Age Of Genomics, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Plasmepsin 4, The Food Vacuole Aspartic Proteinase Found In All Plasmodium Spp. Infecting Man, John B. Dame, Charles A. Yowell, Levi Omara-Opyene, Jane M. Carlton, Roland A. Cooper, Tang Li Jul 2003

Plasmepsin 4, The Food Vacuole Aspartic Proteinase Found In All Plasmodium Spp. Infecting Man, John B. Dame, Charles A. Yowell, Levi Omara-Opyene, Jane M. Carlton, Roland A. Cooper, Tang Li

Roland A. Cooper

Plasmepsins are aspartic proteinases of the malaria parasite, and seven groups of plasmepsins have been identified by comparing genomic sequence data available for the genes encoding these enzymes from Plasmodium falciparum, Plasmodium vivax, Plasmodium knowlesi, Plasmodium berghei, and Plasmodium yoelii. The food vacuole plasmepsins typified by plasmepsin 4 from P. falciparum (PfPM4) constitute one of these groups. Genes encoding the ortholog of PfPM4 have been cloned from Plasmodium ovale, Plasmodium malariae, and P. vivax. In addition, P. falciparum contains three paralagous food vacuole plasmepsins or plasmepsin-like enzymes that appear to have arisen by gene duplication, plasmepsins 1 (PfPM1), 2 (PfPM2) …


Gradual Evolution In Bacteria: Evidence From Bacillus Systematics, M. Feldgarden, N. Byrd, Frederick M. Cohan Dec 2002

Gradual Evolution In Bacteria: Evidence From Bacillus Systematics, M. Feldgarden, N. Byrd, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


What Are Bacterial Species?, Frederick M. Cohan Dec 2001

What Are Bacterial Species?, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Bacterial Species And Speciation, Frederick M. Cohan Dec 2000

Bacterial Species And Speciation, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Bacillus Sonorensis Sp. Nov., A Close Relative Of Bacillus Licheniformis, Isolated From Soil In The Sonoran Desert, Arizona, M. M. Palmisano, L. K. Nakamura, K. E. Duncan, C. A. Istock, Frederick M. Cohan Dec 2000

Bacillus Sonorensis Sp. Nov., A Close Relative Of Bacillus Licheniformis, Isolated From Soil In The Sonoran Desert, Arizona, M. M. Palmisano, L. K. Nakamura, K. E. Duncan, C. A. Istock, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Compartmental Specificity Of Cellular Membrane Fusion Encoded In Snare Proteins, James Mcnew, Frank Parlati, Ryorichi Fukuda, Robert Johnston, Keren Paz, Fabienne Paumet, Thomas Sollner, James Rothman Dec 1999

Compartmental Specificity Of Cellular Membrane Fusion Encoded In Snare Proteins, James Mcnew, Frank Parlati, Ryorichi Fukuda, Robert Johnston, Keren Paz, Fabienne Paumet, Thomas Sollner, James Rothman

Fabienne Paumet

No abstract provided.


Soluble Nsf Attachment Protein Receptors (Snares) In Rbl-2h3 Mast Cells: Functional Role Of Syntaxin4 In Exocytosis And Identification Of A Vamp8-Containing Secretory Compartment, Fabienne Paumet, Joelle Le Mao, Sophie Martin, Thierry Galli, Bernard David, Ulrich Blank, Michele Roa Dec 1999

Soluble Nsf Attachment Protein Receptors (Snares) In Rbl-2h3 Mast Cells: Functional Role Of Syntaxin4 In Exocytosis And Identification Of A Vamp8-Containing Secretory Compartment, Fabienne Paumet, Joelle Le Mao, Sophie Martin, Thierry Galli, Bernard David, Ulrich Blank, Michele Roa

Fabienne Paumet

No abstract provided.


Barriers To Genetic Exchange Between Bacterial Species: Streptococcus Pneumoniae Transformation, J. Majewski, P. Zawadzki, P. Pickerill, Frederick M. Cohan, C. W. Dowson Dec 1999

Barriers To Genetic Exchange Between Bacterial Species: Streptococcus Pneumoniae Transformation, J. Majewski, P. Zawadzki, P. Pickerill, Frederick M. Cohan, C. W. Dowson

Frederick M. Cohan

No abstract provided.


Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof. Jun 1999

Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

The objective of this study was the research of novel and inexpensive sources of polysaccharides degrading enzymes such as amylases and glucanases from local plants in order to justify their biotechnological applications. The isolation of two l3-amylases and an endo-1,3-ß-D-glucanase was reached using common protein purification methods such as buffer extraction, ammonium sulfate fractionation, ionexchange and gel filtration chromatographies. The methods used were simple and easily reproducible, suggesting the possibilfty of large-scale production. ln the crude extract of Curculigo pilosa tuber, only ß-amylase was detected as starch degrading enzyme and its activity was approximately 282 Uig of fresh material. The …


Relationship Between The Bacillus Subtilis Clades Associated With Strains 168 And W23: A Proposal For B. Subtilis Subsp. Subtilis And B. Subtilis Subsp. Spizizenii, L. K. Nakamura, M. S. Roberts, Frederick M. Cohan Dec 1998

Relationship Between The Bacillus Subtilis Clades Associated With Strains 168 And W23: A Proposal For B. Subtilis Subsp. Subtilis And B. Subtilis Subsp. Spizizenii, L. K. Nakamura, M. S. Roberts, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Dna Sequence Similarity Requirements For Interspecific Recombination In Bacillus, J. Majewski, Frederick M. Cohan Dec 1998

Dna Sequence Similarity Requirements For Interspecific Recombination In Bacillus, J. Majewski, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


The Effect Of Mismatch Repair And Heteroduplex Formation On Sexual Isolation In Bacillus, Jacek Majewski, Frederick M. Cohan Dec 1997

The Effect Of Mismatch Repair And Heteroduplex Formation On Sexual Isolation In Bacillus, Jacek Majewski, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank Dec 1996

Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank

Fabienne Paumet

No abstract provided.


Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan Dec 1995

Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.


Propagation Of Adherent Bacteria On The Brittlestar Ophionereis Annulata (Le Conte) After Enrichment Of Seawater With Amino Acids, Michael A. Rice Dec 1987

Propagation Of Adherent Bacteria On The Brittlestar Ophionereis Annulata (Le Conte) After Enrichment Of Seawater With Amino Acids, Michael A. Rice

Michael A Rice

Epidermal surfaces of the ophiouroid Ophionereis annulata (Le Conte) were examined by scanning electron microscopy. Microvilli, which are characteristic of many transport epithelia, are present on the epidermal surfaces. Epidermal surfaces of freshly-collected brittlestars were compared with brittlestars that were maintained in the laboratory for 48 hours in fresh seawater and in seawater enriched with 20uM glycine. Very few bacteria were observed on the surface of the freshly-collected animals or on animals maintained in the laboratory in fresh seawater. The epidermal surfaces of animals maintained in seawater enriched with 20 uM glycine showed extensive mats of coccoid and bacilloid bacteria. …


Extracellular Enzyme Production By Rhizopus And Mucor Species On Solid Media, D P. Thompson, Broderick Eribo Dec 1983

Extracellular Enzyme Production By Rhizopus And Mucor Species On Solid Media, D P. Thompson, Broderick Eribo

Broderick Eribo

Solid media were employed to determine the presence and absence of extracellular enzyme production by two genera of fruit-rot fungi, Rhizopus and Mucor. The results of this investigation revealed that phosphatase was released into the cultural medium by all the fungi examined; however, only R. oryzae, R. tritici, M. mucedo, and M. piriformis showed the possibility of being high producers of the enzyme. Protease, urease, ribonuclease, pectate lyase, and polygalacturonase, at varying levels of activity, were detected, in the majority of the fungi, in the cultural medium.


Fungal Metabolite From Members Of The Genus Rhizopus, D P. Thompson, A W. Smalley, Broderick Eribo Apr 1981

Fungal Metabolite From Members Of The Genus Rhizopus, D P. Thompson, A W. Smalley, Broderick Eribo

Broderick Eribo

A fluorescent metabolite present in seven members of the genus Rhizopus was isolated. This compound appeared green before spray treatment and purple after spray treatment with p-anisaldehyde in visible light. Subsequent purification and structural elucidation of the isolated compound yielded 1-[2,6,10,14-tetramethyl-17-carbomethyl heptadecyl]-1-[2,6,10,14-tetramethyl-17-methanoyl heptadecyl]-benzene.


Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore Jul 1978

Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore

Victor R. Ambros

A protein similar to that previously demonstrated on poliovirus RNA and replicative intermediate RNA (VPg) was found on all sizes of nascent viral RNA molecules and on the polyuridylic acid isolated from negative-strand RNA. 32P-labeled nascent chains were released from their template RNA and fractionated by exclusion chromatography on agarose. Fingerprint analysis using two-dimensional polyacrylamide gels of RNase T1 oligonucleotides derived from nascent chains of different lengths showed that a size fractionation of nascent chains was achieved. VPg was recovered from nascent chains varying in length from 7,500 nucleotides (full-sized RNA) to about 500 nucleotides. No other type of 5' …