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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Genome Sequence Of Lactobacillus Pentosus Kca1: Vaginal Isolate From A Healthy Premenopausal Woman, Kingsley Anukam
Genome Sequence Of Lactobacillus Pentosus Kca1: Vaginal Isolate From A Healthy Premenopausal Woman, Kingsley Anukam
Kingsley C Anukam
The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum) from the vagina of a healthy Nigerian woman. The genome was sequenced using Illumina GA II technology. The resulting 16,920,226 paired-end reads were assembled with the Velvet tool. Contigs were annotated using the RAST server, and manually curated. A comparative analysis with the available genomes of L. pentosus IG1 and L. plantarum WCFS1 showed that over 15% …
Chapter 20:The Impact Of Probiotics On Maternal And Child Health: Clinical Evidence., Kingsley Anukam, Gregor Reid
Chapter 20:The Impact Of Probiotics On Maternal And Child Health: Clinical Evidence., Kingsley Anukam, Gregor Reid
Kingsley C Anukam
Key Points • Alleviating the problems of maternal and child health in countries with large malnourished and/or HIV-infected populations, will require multidimensional approaches including holistic and pharmaceutical interventions. • Urogenital infections have a major role in preterm labor and the well-being of newborns. • Probiotics show potential in reducing the risk of recurrence of urinary tract infection (UTI). • There is some evidence to suggest that probiotics can have a role to play in bacterial vaginosis (BV) and maternal health. • The role of probiotics in a number of newborn, and childhood diseases is reviewed in this chapter.
Probiotics: 100 Years (1907-2007) After Elie Metchnikoff's Observations, Kingsley Anukam, Gregor Reid
Probiotics: 100 Years (1907-2007) After Elie Metchnikoff's Observations, Kingsley Anukam, Gregor Reid
Kingsley C Anukam
No abstract provided.
Organisms Associated With Bacterial Vaginosis In Nigerian Women As Determined By Pcr-Dgge And 16s Rrna Gene Sequence, Kingsley Anukam, Gregor Reid
Organisms Associated With Bacterial Vaginosis In Nigerian Women As Determined By Pcr-Dgge And 16s Rrna Gene Sequence, Kingsley Anukam, Gregor Reid
Kingsley C Anukam
Background: Bacterial vaginosis (BV) is a condition with diverse etiology. This condition predisposes women to increased susceptibility to sexually transmitted diseases, including human immunodeficiency virus (HIV) infections and preterm birth. The diagnostic methods currently adopted in the evaluation of patient samples for BV are arguably Amsel criteria, and Nugent score that require microscopy and expert interpretation. These two methods are still subjective. Objective: The objective of this study was to determine the organisms present in the vagina of 34 HIV negative Nigerian women diagnosed as having bacterial vaginosis by using molecular techniques. Methods: The vaginal samples were subjected to DNA …
Lactobacillus Vaginal Microbiota Of Women Attending A Reproductive Health Care Service In Benin City, Nigeria., Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
Lactobacillus Vaginal Microbiota Of Women Attending A Reproductive Health Care Service In Benin City, Nigeria., Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
Kingsley C Anukam
OBJECTIVE: The objective of this study was to determine whether Lactobacillus species found in African women differ substantially to those of Caucasian decent, described in previous studies. The vaginal microbiota play an important role in female health, and when the naturally dominant lactobacilli are displaced resulting in bacterial vaginosis (BV), the host is more at risk of acquiring sexually transmitted diseases, including HIV. METHODS: Vaginal samples were collected from 241 healthy, pre-menopausal Nigerian women, which were then Gram stained for Nugent scoring. Microbial DNA was extracted, amplified using PCR and Lactobacillus primers, and processed by Denaturing Gradient Gel Electrophoresis (DGGE). …
16s Rrna Gene Sequence And Phylogenetic Tree Of Lactobacillus Species From The Vagina Of Healthy Nigerian Women, Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
16s Rrna Gene Sequence And Phylogenetic Tree Of Lactobacillus Species From The Vagina Of Healthy Nigerian Women, Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid
Kingsley C Anukam
Lactobacilli are ubiquitous in nature and in humans they play a very significant role in the general health maintenance of the host. Identification of Lactobacilli has previously been based on cultutre-dependent methods and recently molecular techniques involving gene sequencing are now the ‘gold standard’. Scarce information exists in Africa on the real identity of Lactobacillus species, albeit phylogenetic distances among the species present in the human vagina. 185 vaginal swabs were collected from healthy premenopausal women (18-48 years). Bacterial DNA was extracted, amplified using PCR, with group specific Lactobacillus primers, and processed by denaturing gradient gel electrophoresis (DGGE). DGGE bands …