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- Cytotoxicity (7)
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- Bacillus thuringiensis (2)
- Biomaterials (2)
- Caspase-3 (2)
- Essential oil (2)
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- Gene expression (2)
- Laccase (2)
- Maize (2)
- NADPH oxidase (2)
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- Pichia pastoris (2)
- Platelets (2)
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- 5'-deletion fragments (1)
- 79 AD eruption of Vesuvius (1)
- ADMET analysis (1)
Articles 31 - 60 of 95
Full-Text Articles in Life Sciences
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
Turkish Journal of Biology
The aim of this study was to increase laccase production in Trametes versicolor ATCC 200801 pellets by using various inducers and their combinations under repeated-batch conditions. Because copper is an effective inducer for laccase production, the effect of Cu on laccase production in this strain was tested first. The optimal Cu concentration for the highest laccase production was 0.5 mM. Following this preliminary study, the effect of other inducers [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), syringaldazine, guaiacol, and 2,5-xylidine] on laccase production was determined. Copper was determined to be the most efficient inducer among the inducers used. Therefore, the synergistic effect of each …
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Turkish Journal of Biology
n thermally damaged archaeological bones the quantity, quality, and amplifiability of DNA are very much reliant on both the extent of heating and the environmental conditions of the burial context. In this study we tested the possibility of extracting and amplifying ancient DNA from human bone remains of Herculaneum victims of the 79 AD eruption of Vesuvius using a combination of histochemical and molecular methods. Long bone samples with variable degrees of chromatic and morphological alterations consistent with exposure to temperatures of about 300 °C were taken from four specimens. Using histochemical stains, bone cryostat sections from three individuals revealed …
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Turkish Journal of Biology
Exogenous proline (PRO) at low concentrations can enhance drought stress tolerance in different application modes such as application to rooting medium, foliar spray, and seed soaking. However, there is no information about which application mode is more effective for increasing the drought tolerance. Comparative effects of 1, 10, and 20 mM PRO applications through three application modes to hydroponically grown seedlings were examined under short-term drought stress in maize seedlings. Effects on leaf water potential, membrane damage, chlorophyll content, proline level, and gas exchange parameters such as net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (gs), and substomatal CO2 …
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Turkish Journal of Biology
Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium …
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Turkish Journal of Biology
Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in …
Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z
Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z
Turkish Journal of Biology
Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. …
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
Turkish Journal of Biology
Direct reprogramming of pancreatic beta cells is of great interest due to its possible use in diabetes treatment. Various studies have demonstrated direct reprogramming of insulin expressing beta cells from other somatic cells by overexpressing important pancreatic transcription factors through viral vectors. However, concerns about using viral vectors in the clinic have prompted scientists to find new approaches for gene delivery and expression. In vitro synthesis and transfection of mRNAs is a safer strategy for ectopic gene expression. In this study we aimed to design and in vitro synthesize human pancreatic transcription factors' mRNAs: Pdx1, Ngn3, and MafA. We also …
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Turkish Journal of Biology
Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposide-loaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Turkish Journal of Biology
The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the …
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Turkish Journal of Biology
Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of …
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Turkish Journal of Biology
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
Turkish Journal of Biology
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and …
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Turkish Journal of Biology
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Turkish Journal of Biology
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant …
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Turkish Journal of Biology
Galectins (gals) are ß-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin …
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Turkish Journal of Biology
Moroctocog alpha is a human B-domain deleted recombinant factor VIII (BDDrFVIII), which represents a new generation of pure antihemophilic products. We describe here an optimized procedure for polyclonal anti-FVIII-antibody production with the use of BDDrFVIII as an immunogen. The main immunochemical characteristics of the produced antibodies and their potential biomedical applications are also reported. Rabbits were immunized with BDDrFVIII as an emulsion with Freund's adjuvant or with antigen immobilized in polyacrylamide gel (PAAG). Antibody titers in immune sera were assayed by enzyme-linked immunosorbent assay (ELISA). IgG purification was performed by affine chromatography on protein A-sepharose. Immune sera and IgG were …
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Turkish Journal of Biology
The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. Cotton plants were grown with different concentrations of MgSO4 in hydroponic medium to create nutrient deficiency (0, 75, 150, 1000 μM Mg). Gradual decreases in growth and photosynthetic rates were observed with declining Mg concentrations and 0, 75, and …
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Turkish Journal of Biology
Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 …
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Turkish Journal of Biology
Histone deacetylases (HDACs) are enzymes that act on histone proteins to remove the acetyl group and thereby regulate the chromatin state. HDACs act not only on histone protein but also nonhistone proteins that are key players in cellular processes such as the cell cycle, signal transduction, apoptosis, and more. "Classical" HDACs have been shown to be promising targets for anticancer drug design and development. However, the selectivity of HDAC inhibitors for HDAC isoforms remains the motivation of current research in this field. Here, we explored Class I HDACs and HDAC6 by sequence alignment and structural superimposition, catalytic channel extraction, and …
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Turkish Journal of Biology
In the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ΔΔCT method. At the last stage of the study, we …
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Turkish Journal of Biology
The study presented here aims to elucidate the effects of emodin (EO = 1,3,8-trihydroxy-6-methylanthraquinone) in its free form and when loaded into a mesoporous silica nanocarrier SBA-15 (→ SBA-15 EO) on the activities of the main antioxidative enzymes, superoxide dismutase, catalase, glutathione S-transferase, and glutathione reductase, in larvae of a polyphagous insect pest, the browntail moth Euproctis chrysorrhoea (L.). The results show that only SBA-15 EO upregulates the activities of the tested antioxidative enzymes. These results point to significant differences in the effectiveness of the compound in the free versus the loaded form.
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Turkish Journal of Biology
Lead (Pb) has wide-ranging effects on various essential physiological processes in plants including seed germination, root/shoot growth, photosynthetic efficiency, water status, and activities of enzymes. The effect of combined treatment of 24-epibrassinolide (10-7 M, EBL) and salicylic acid (1 mM, SA) on growth, photosynthetic attributes, and phenolic compounds in 30-, 60-, and 90-day-old plants of Brassica juncea L. under Pb stress (0.25 mM, 0.50 mM, and 0.75 mM) were studied. It was observed that Pb toxicity resulted in lowered growth and photosynthetic efficiency. The expressions of CHLASE, PSY, CHS, and PAL genes were altered. Presoaking treatment with the combination of …
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology. Since most enterohemorrhagic infections result from water and food contaminations of Escherichia coli O157:H7, selective and rapid detection of this organism in environmental and biological complexes is necessary. In this study, optimized parameters of antibody (Ab)-based LFA for rapid detection of pathogenic E. coli O157:H7 are described. GNPs were used as visualizing agents. The measuring parameters include the Ab concentration …
The Sca1+ Mesenchymal Stromal Subpopulation Promotesdendritic Cell Commitment In The Niche, Na Li, Dandan Yin, Huijie Zhang, Jinmei Xu, Fan Wen, Zheng Liu, Yaozhen Chen, Ning An, Jiajia Xin, Yazhou Wang, Wen Yin, Xingbin Hu
The Sca1+ Mesenchymal Stromal Subpopulation Promotesdendritic Cell Commitment In The Niche, Na Li, Dandan Yin, Huijie Zhang, Jinmei Xu, Fan Wen, Zheng Liu, Yaozhen Chen, Ning An, Jiajia Xin, Yazhou Wang, Wen Yin, Xingbin Hu
Turkish Journal of Biology
The hematopoietic microenvironment regulates self-renewal and differentiation of hematopoietic stem cells. Mesenchymal stromal cells (MSCs) contribute to the niche and participate in supporting dendritic cell (DC) commitment in vitro and in vivo. However, due to MSCs being heterogenic, it is necessary to understand the function of the MSC subpopulation in modulating the DC commitment. The current study showed that one of the bone-related Sca1+ MSCs enhanced bone marrow cell differentiation into DCs by direct cell-to-cell contact. Furthermore, the expression of STAT3 and STAT5 genes decreased in supernatant cells after Sca1+ MSCs were cocultured with bone marrow cells. In contrast, the …
Cloning, Expression, And Characterization Of Human Brain Acetylcholinesterase In Escherichia Coli Using A Sumo Fusion Tag, Hami̇d Ceylan, Orhan Erdoğan
Cloning, Expression, And Characterization Of Human Brain Acetylcholinesterase In Escherichia Coli Using A Sumo Fusion Tag, Hami̇d Ceylan, Orhan Erdoğan
Turkish Journal of Biology
The molecular structure of acetylcholinesterase (AChE) attracts interest because of its versatility and significant role in the cholinergic system. The main purpose of the present study was to clone a full-length cDNA sequence of human brain acetylcholinesterase (hAChE) into pET SUMO vector and express it successfully. The integrity of the constructed plasmid was confirmed by cross PCR. This recombinant construct was expressed in Escherichia coli BL21 (DE-3). In this work, we produced hexahistidine (6xHis) tagged fusion protein by isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using nickel (Ni2+) affinity chromatography. Using anti-His antibody, we detected ~90 kDa fusion protein. The expression …
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Turkish Journal of Biology
Human mesenchymal stromal cells (MSCs) from different parts of the body (i.e. bone marrow, BM) have distinct cellular and molecular features including global gene expression profiles. Quantitative polymerase chain reaction is a reliable method used in the quantification of gene expression. Correct assessment of target gene expression mostly depends on the reference gene (RG) of choice. Herein, expression levels of RGs (n = 19) in adipo-/osteogenic-differentiated and control (uninduced) BM-MSCs from donors were measured using the RealTime ready Human Reference Gene Panel. Characterization of BM-MSCs was assessed using in vitro differentiation by histochemical staining and immunophenotyping of cells by flow …
Preparation, Characterization, And Enhanced Antimicrobial Activity: Quercetin-Loaded Plga Nanoparticles Against Foodborne Pathogens, Tüli̇n Arasoğlu, Serap Derman, Banu Mansuroğlu, Deni̇z Uzunoğlu, Büşra Sümaye Koçyi̇ği̇t, Büşra Gümüş, Tayfun Acar, Burcu Tuncer
Preparation, Characterization, And Enhanced Antimicrobial Activity: Quercetin-Loaded Plga Nanoparticles Against Foodborne Pathogens, Tüli̇n Arasoğlu, Serap Derman, Banu Mansuroğlu, Deni̇z Uzunoğlu, Büşra Sümaye Koçyi̇ği̇t, Büşra Gümüş, Tayfun Acar, Burcu Tuncer
Turkish Journal of Biology
The use of quercetin as a bioflavonoid is becoming increasingly common in food industries even though poor water solubility, instability, absorption, and permeability have limited its application. The oil-in-water single-emulsion solvent evaporation method to synthesize highly stable and soluble quercetin-encapsulated nanoparticles (NPs), in which the reaction yield, particle size, and polydispersity of the NPs are varied greatly within the process parameters of the synthesis method, has been optimized. NPs with different initial quercetin amounts were used to determine how the quercetin amount affected nanoparticle properties and antimicrobial eficiency. Listeria monocytogenes, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus were chosen as …
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Turkish Journal of Biology
The use of transition metal complexes as appropriate analogues to cisplatin and similar anticancer drugs nowadays is of utmost importance. The inertness of Pt(IV), Pd(II), and Rh(III) complexes and the choosing of convenient ligands could offer a possible advantage in comparison to Pt(II) complexes. These advantages could be attributed to the better anticancer activity and fewer possible side effects. In this paper, we have chosen three complexes of different transition metal ions, [(TLtBu)PdIICl]ClO4, [RhIII(LH2tBu)Cl3], and [PtIV(LH2tBu)Cl3]Cl (where TLtBu is 2,6-bis[(1,3-di-tert-butylimidazolin-2-imino)methyl]pyridine and LH2tBu is 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine), for biological tests. All three complexes exerted strong prooxidative and cytotoxic effects on the human colorectal …
Molecular Investigations Of The Somatic Embryogenesis Recalcitrancein The Cherry (Prunus Cerasus L.) Rootstock Cab 6p, Kaouther Ben Mahmoud, Emna Jedidi, Fabienne Delporte, Yordan Muhovski, Ahmed Jemmali, Philippe Druart
Molecular Investigations Of The Somatic Embryogenesis Recalcitrancein The Cherry (Prunus Cerasus L.) Rootstock Cab 6p, Kaouther Ben Mahmoud, Emna Jedidi, Fabienne Delporte, Yordan Muhovski, Ahmed Jemmali, Philippe Druart
Turkish Journal of Biology
Somatic embryogenesis is a powerful tool for clonal propagation and plant breeding in many plant species, but its application is limited by the genotype response based on the interaction between gene expression and culture conditions. In this context, the recalcitrance of the nonembryogenic cherry rootstock Prunus cerasus cultivar CAB 6P was investigated via the analysis of the candidate genes PiABP19, Picdc2, and PiSERK3 in comparison with the embryogenic genotype Prunus incisa cultivar N°131. Semiquantitative RT-PCR was employed to assess the transcript level of these genes in leaf explants and derived calli of the two genotypes cultured on picloram-containing MS medium …
Identification Of Proteins Differentially Accumulated In Enterococcus Faecalis Under Acrylamide Exposure, Aleksandra Bocian, Konrad Hus, Marcin Jaromin, Miroslaw Tyrka, Andrzej Lyskowski
Identification Of Proteins Differentially Accumulated In Enterococcus Faecalis Under Acrylamide Exposure, Aleksandra Bocian, Konrad Hus, Marcin Jaromin, Miroslaw Tyrka, Andrzej Lyskowski
Turkish Journal of Biology
Analysis of bacterial proteomes can be used to obtain large amounts of information about adaptive microbial mechanisms to changing extracellular conditions. In the past, many bacterial species with the ability to degrade acrylamide were isolated. In this study differences in the Enterococcus faecalis proteome upon acrylamide exposure were investigated. We revealed substantial changes in the proteome of bacteria cultured in different environmental conditions. Microorganisms exposed to acrylamide showed higher accumulation of proteins associated with energy metabolism and its regulation. Moreover, several proteins involved in protection of cells from stress conditions were also identified. These biomacromolecules are involved in proper folding …