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Fret-Based Characterization Of K264a, D345a, And Y335amutants In The Human Dopamine Transporter, Oya Orun, Pinar Mega Ti̇ber
Fret-Based Characterization Of K264a, D345a, And Y335amutants In The Human Dopamine Transporter, Oya Orun, Pinar Mega Ti̇ber
Turkish Journal of Biology
The dopamine transporter (DAT) plays a role in the termination of dopaminergic neurotransmission; thereby it is accepted as the primary target of various psychostimulants. N-terminal phosphorylation of DAT has been proposed as a regulator in different DAT functions, such as amphetamine-induced efflux or PKC/PKA-mediated responses. To understand the role of N-terminal conformational changes in dopamine transporter structure and function, the fluorescence resonance energy transfer (FRET) method was applied to various DAT constructs fluorescently labeled at the N-terminus and substrate-induced conformational changes were determined using rhodamine-labeled cocaine analog JHC1-64 in three DAT mutants. The results indicated that the construct with YFP …
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Turkish Journal of Biology
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …