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Full-Text Articles in Life Sciences
The Application Of Mesenchymal Progenitor Stem Cells For The Reduction Of Oxidative Stress In Animals, Edyta Wojtas, Andrzej Zachwieja, Anna Zwyrzykowska, Robert Kupczynski, Krzysztof Marycz
The Application Of Mesenchymal Progenitor Stem Cells For The Reduction Of Oxidative Stress In Animals, Edyta Wojtas, Andrzej Zachwieja, Anna Zwyrzykowska, Robert Kupczynski, Krzysztof Marycz
Turkish Journal of Biology
Oxidative stress is the primary cause of lowering of the body's immune status. It arises as a result of intense metabolic processes in humans and animals that involve a decrease in antioxidant enzymes activity at the expense of increased production of free radicals and reactive oxygen species (ROS). Consequently, this leads to oxidation of lipids, proteins, DNA, and RNA, causing cell proliferation reduction, faster cell aging, and subsequent degradation. Currently, there is a search for methods to maintain an oxidation-antioxidant balance. The results of many authors' research point to the effectiveness of the use of mesenchymal stem cells (MSCs) in …
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Turkish Journal of Biology
The characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. The expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 …
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Turkish Journal of Biology
For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …