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Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Turkish Journal of Biology
Leaf rolling (LR), particularly in crops, is a dehydration avoidance mechanism that results from water loss in plants under drought conditions. Two maize cultivars (Zea mays L.) differing in their tolerance to drought (cultivar Side was selected as stress-tolerant and cultivar Karaçay was selected as stress-sensitive) under PEG-induced drought stress were used in this study to understand the effects of polyamines (PAs) on LR response and the role of the antioxidant system in the delayed LR process. Aerial parts of the seedlings were excised prior to the experiment and then submerged in a Hoagland nutrient solution with or without 0.1 …
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Turkish Journal of Biology
Plant defensins are multifunctional small cysteine-rich proteins. They are active against fungi, bacteria, and many viruses, and they inhibit trypsin and α-amylase activities. In this study, we expressed the maize defensin gene (ZmDEF1) in tobacco seeds in order to establish the basis for generating transgenic maize plants resistant to weevils. The ZmDEF1 gene was isolated from Maison, a Vietnamese local maize cultivar, which is well known for having the highest resistance to weevils among other local cultivars. The ZmDEF1 gene was cloned into a binary vector, pBetaPhaso-dest, which carries phaseolin, a seed-specific promoter, to direct defensin expression in tobacco seeds. …