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Full-Text Articles in Life Sciences
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Turkish Journal of Biology
Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5'-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cis-acting regulatory DNA element database. There exist core promoter regions including …
Effect Of Calprotectin Subunit S100a9 On The Expression And Methylation Of Ocln In Human Melanoma Cell Line A-375, Mostafa Najafi, Safar Ali Alizadeh, Mehdi Azad, Taghi Naserpour Farivar, Farzad Rajaei, Kourosh Hotam Sorouri, Babak Rahmani, Nematollah Gheibi
Effect Of Calprotectin Subunit S100a9 On The Expression And Methylation Of Ocln In Human Melanoma Cell Line A-375, Mostafa Najafi, Safar Ali Alizadeh, Mehdi Azad, Taghi Naserpour Farivar, Farzad Rajaei, Kourosh Hotam Sorouri, Babak Rahmani, Nematollah Gheibi
Turkish Journal of Biology
Increased levels of calprotectin subunits S100A8 and S100A9 have been detected in human cancers. Melanoma is the most aggressive type of skin cancer, and its treatment is challenging because of its brain metastasis. OCLN encodes occludin, which plays a major role in the formation and regulation of tight junctions. The aim of this study was to evaluate the methylation status of the OCLN promoter and its expression in A-375 melanoma cells treated with or without various concentrations of S100A9 for 24, 48, and 72 h. Total RNA was extracted, and synthesized cDNA was assessed by performing real-time PCR. MSP-PCR was …