Life Sciences Commons^™

Electrically Mediated Plasmid Dna Delivery To Hepatocellular Carcinomas In Vivo, L. Heller, M. J. Jaroszeski, D. Coppola, C. Pottinger, R. Gilbert, Richard Heller

Bioelectrics Publications

Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.

Studies On The Roles Of Atp In Nitrogenase Catalysis, Wei Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitrogenase is the enzyme that catalyzes the reduction of nitrogen to ammonia in a reaction requiring MgATP hydrolysis. Two component proteins of nitrogenase are the iron protein (Fe protein) and the molybdenum-iron protein (MoFe protein).

Nitrogenase contains two nucleotide binding sites. During catalysis, the Fe protein binds two MgATP first. The conformational changes induced upon MgATP binding allow the Fe protein to associate with the MoFe protein. After the formation of the Fe protein-MoFe protein complex, a single electron is transferred from the Fe protein to the MoFe protein, an event that is coupled to MgATP hydrolysis in the Fe …

Comparison Of The Mutant Frequency And Mutation Spectra Of Three Non-Genotoxic Carcinogens Oxazepam, Phenobarbital, And Wyeth 14,643 At The Λcii Locus In Big Blue Transgenic Mice, Vijaypreet K. Singh

Honors Theses

No abstract provided.

Test Of Intron Predictions Reveals Novel Splice Sites, Alternatively Spliced Mrnas And New Introns In Meiotically Regulated Genes Of Yeast, Carrie Davis, Leslie Grate, Marc Spingola, Manuel Ares

Biology Department Faculty Works

Correct identification of all introns is necessary to discern the protein-coding potential of a eukaryotic genome. The existence of most of the spliceosomal introns predicted in the genome of Saccharomyces cerevisiae remains unsupported by molecular evidence. We tested the intron predictions for 87 introns predicted to be present in non-ribosomal protein genes, more than a third of all known or suspected introns in the yeast genome. Evidence supporting 61 of these predictions was obtained, 20 predicted intron sequences were not spliced and six predictions identified an intron-containing region but failed to specify the correct splice sites, yielding a successful prediction …

Test Of Intron Predictions Reveals Novel Splice Sites, Alternatively Spliced Mrnas And New Introns In Meiotically Regulated Genes Of Yeast, Carrie A. Davis, Leslie Grate, Marc Spingola, Manuel Ares

Marc Spingola

Method And Apparatus For Three-Dimensional Ultrasound Imaging Of Biopsy Needle, Syed Omar Ishrak, Mir Said Seyed-Bolorforosh, William Thomas Hatfield, Todd Michael Tillman, Brian Peter Geiser, Gregory R. Bashford, Michael Joseph Washburn

Biomedical Imaging and Biosignal Analysis Laboratory

A method and an apparatus for three-dimensional ultrasound imaging of a needle-like instrument, such as a biopsy needle, inserted in a human body. The instrument is visualized by transmitting ultrasound beams toward the instrument and then detecting the echo signals using a linear array of transducer elements. The problem of ultrasound being reflected from a biopsy needle in a direction away from the transducer array is solved by steering the transmitted ultrasound beams t increase the angle at which the beams impinge upon the biopsy needle. Ideally the ultrasound beams are perpendicular to the needle. This increases the system's sensitivity …

Molecular Evolution Of Insecticidal Spore-Forming Bacteria, John Pool

Honors Theses

Molecular methods are increasingly being used to determine the phylogeny of microorganisms. This research was intended to determine phylogenetic relationships for bacteria of the species Bacillus thuringiensis and other members of the Bacillus cereus group. Each strain was analyzed by its sasp-B gene sequence to determine its species classification and relation to other strains studied. Results of this study indicated that according to the sasp-B gene tree, the species Bacillus thuringiensis is a paraphyletic with respect to both Bacillus cereus and Bacillus anthracis. Some unexpected results and implications for species designations are also discussed.

Effect Of Chlamydia Trachomatis Infection And Subsequent Tnfa Secretion On Apoptosis In The Murine Genital Tract, Jean-Luc Perfettini, Toni Darville, Gabriel Gachelin, Philippe Souque, Michel Huerre, Alice Dautry-Varsat, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

The pathology observed during Chlamydia infection is due initially to localized tissue damage caused by the infection itself, followed by deleterious host inflammatory responses that lead to permanent scarring. We have recently reported that the infection byChlamydia in vitro results in apoptosis of epithelial cells and macrophages and that infected monocytes secrete the proinflammatory cytokine interleukin-1β. At the same time, proinflammatory cytokines such as tumor necrosis factor alpha (TNF-α) can also trigger apoptosis of susceptible cells. To study the possible relationship between Chlamydia trachomatis infection and apoptosis in vivo, we used the terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling technique to …

Characterization Of Human T-Cell Leukemia Virus 1 Reverse Transcriptase, Pinky Gundayao Agbuya

Theses and Dissertations in Biomedical Sciences

Human T-cell leukemia virus 1 (HTLV-1) is a type C human retrovirus which has been the causative agent of Adult T-cell leukemia. Replication of the retrovirus; requires a reverse transcriptase which converts the retroviral RNA into DNA which is later incorporated into the host's genome. Very little is known about the reverse transcriptase of HTLV-1. Researchers have attempted to purify HTLV-1 RT by isolating the enzyme from human cell lines. Because large amounts of protein could not be produced by this isolation method, the reverse transcriptase cannot be fully characterized. In this research, a recombinant protein expressed in E. coli …

Xenopus Laevis Gelatinase B (Xmmp-9): Development, Regeneration, And Wound Healing, Brian Walter, Maria Carinato, Jonathan Henry

Brian Walter

It has been argued that matrix metalloproteinases play important roles in cellular differentiation and regeneration in certain systems. While studying changes in gene expression associated with the phenomena of cornea/lens transdifferentiation ("lens regeneration"), which takes place in the larva of Xenopus laevis, we identified the Xenopus gelatinase B gene. The open reading frame is homologous to other gelatinase B genes identified in other species and encodes all of the domains characteristic of this protein. Xenopus gelatinase B (Xmmp-9) is first expressed during early tail-bud stages in a subset of mesodermal cells scattered throughout the body. Expression is also seen in …

Novel Function Of Phosphoinositide 3-Kinase In T Cell Ca2+ Signaling, Ao-Lin Hsu, Tsui-Ting Ching, Goutam Sen, Da-Sheng Wan, Subbarao Bondada, Kalwant S. Authi, Ching-Shih Chen

Pharmaceutical Sciences Faculty Publications

This study presents evidence that phosphoinositide (PI) 3-kinase is involved in T cell Ca²⁺ signaling via a phosphatidylinositol 3,4,5-trisphosphate PI(3,4,5)P₃-sensitive Ca²⁺entry pathway. First, exogenous PI(3,4,5)P₃ at concentrations close to its physiological levels induces Ca²⁺ influx in T cells, whereas PI(3,4)P₂, PI(4,5)P₂, and PI(3)P have no effect on [Ca²⁺]_i. This Ca²⁺ entry mechanism is cell type-specific as B cells and a number of cell lines examined do not respond to PI(3,4,5)P₃ stimulation. Second, inhibition of PI 3-kinase by wortmannin and by overexpression of the …

Use Of Biotin-Labeled Nucleic Acids For Protein Purification And Agarose-Based Chemiluminescent Electromobility Shift Assays., Joseph Rodgers, Pinky Patel, Jason Hennes, Sara Bolognia, David Mascotti

David P. Mascotti

We have employed biotin-labeled RNA to serve two functions. In one, the biotin tethers the RNA to streptavidin–agarose beads, creating an affinity resin for protein purification. In the other, the biotin functions as a label for use in a modified chemiluminescent electromobility shift assay (EMSA), a technique used to detect the formation of protein–RNA complexes. The EMSA that we describe avoids the use not only of radioactivity but also of neurotoxic acrylamide by using agarose as the gel matrix in which the free nucleic acid is separated from protein–nucleic acid complexes. After separation of free from complexed RNA in agarose, …

Multi-Pass Reflectron Time-Of-Flight Mass Spectrometer, Curtiss D. Hanson

Patents (University of Northern Iowa)

A novel design for a time-of-flight mass spectrometer capable of tandem mass spectrometry measurements with high resolution and high sensitivity using two variable reflectrons in a co-linear geometry. Variably switched reflectrons are oriented coaxially on opposing ends of the ion flight region allowing multiple passes of the ions along the flight region permitting high resolution, tandem mass spectrometry experiments to be performed. An electrostatic particle guide is incorporated to ensure high ion transmission efficiency in a multi-pass system. In addition to permitting the high transmission efficiency of ions, the EPG can be used in a bipolar pulsed mode to isolate …

Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

Cell volume regulation is a property present in most animal cell lineages that allows them to recover their original volume after events of swelling or shrinkage. Such events can be caused by changes in external osmolarity or to osmotic gradients generated during normal cell functioning.4,6 The mechanism of cell volume regulation involves transmembrane fluxes of osmotically active solutes in the necessary direction to counteract the net gain or loss of intracellular water.9 The process through which cells recover their normal volume after swelling is named Regulatory Volume Decrease (RVD). This consists of the efflux of inorganic osmolytes, such as K …

The Effects Of Low Ph On Sunfish Assemblages In Southeast Georgia, William Boyd Tate

Legacy ETDs

No abstract provided.

Effects Of Height And Vegetation On Success Of Bird Nests In Maritime Forests, Sharon M. Defalco

Legacy ETDs

Nest predation is an important source of mortality in songbirds and may contribute to declines in Neotropical migrants. I used artificial nests baited with fresh Japanese Quail and Zebra Finch eggs in conjunction with observations of natural nests to quantity the ettects ol nest height and vegetation on nest predation on St. Catherine's Island, a barrier island in southeast Georgia. Because of intense browsing by white-tailed deer. I predicted that lower, more exposed nests would be least successful. Artificial nests (n = 389) were placed in the field, and natural nests (n = 49) were observed, durintz April and May …

Cloning And Sequencing Of Sodium-Hydrogen Exchanger In The Gills Of The Long-Horned Sculpin (Myoxocephalus Octodecimspinosus), Danielle Lee Gunning

Legacy ETDs

No abstract provided.

Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee

Department of Biochemistry: Faculty Publications

HilA activates the expression of Salmonella enterica serovar Typhimurium invasion genes. To learn more

about regulation of hilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In

addition to expected mutations, we identified Tn5 insertions in pstS, fadD, flhD, flhC, and fliA. Analysis of the

pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence

of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the

PhoR-PhoB two-component regulatory system, suggesting that hilA expression may …

Eph Receptors And Ephrins, Masaru Nakamoto

Biology Faculty Publications

The Eph receptors are the largest known family of receptor tyrosine kinases. The Eph receptors and theirmembrane-attached ligands, ephrins, show diverse expression patterns during development. Recent studies havedemonstrated that Eph receptors and ephrins play important roles in many developmental processes, includingneuronal network formation, the patterning of the neural tube and the paraxial mesoderm, the guidance of cellmigration, and vascular formation. In the nervous system, Eph receptors and ephrins have been shown to act aspositional labels to establish topographic projections. They also play a key role in pathway ®nding by axons andneural crest cells. The crucial roles of Eph receptors and …

Endocrine Responses To Chronic Androstenedione Intake In 30- To 56-Year-Old Men, Gregory A. Brown, Matthew D. Vukovich, Emily R. Martini, Marian L. Kohut, Warren D. Franke, David A. Jackson, Douglas S. King

Warren D Franke

In young men, chronic ingestion of 100 mg androstenedione (ASD), three times per day, does not increase serum total testosterone but does increase serum estrogen and ASD concentrations. We investigated the effects of ASD ingestion in healthy 30- to 56-yr-old men. In a double-blind, randomly assigned manner, subjects consumed 100 mg ASD three times daily (n 5 28), or placebo (n 5 27) for 28 days. Serum ASD , dihydrotestosterone (DHT), free and total testosterone, estradiol, prostate-specific antigen (PSA), and lipid concentrations were measured at week 0 and each week throughout the supplementation period. Serum total testosterone and PSA concentrations …

Determining The Phylogenetic Relationships Among The Northern Pike (Esox Lucius), The Muskellunge (Esox Masquinongy), And The Silver Pike, Steven W. Foster

Theses, Dissertations and Capstones

Prioritization of taxa has become a necessity due to limited resources available for conservation. Conservation efforts are often concentrated on those threatened and endangered species with the highest taxonomic importance. The silver pike is a rare game fish, often accounting for less than 1% of a total pike population where it is found. Although silver pike are normally found in association with northern pike, they have distinct morphological characteristics and have not been shown to hybridize with the northern pike. Still, many fisheries biologists consider the silver pike to be a color variant of the northern pike, and to date …

Response Properties Of Tibial Campaniform Sensilla On The Cockroach Leg In Restrained Preparations And Freely Moving Animals, Angela L. Ridgel

Theses, Dissertations and Capstones

The ability to detect changes in forces is important for effective use of a leg in posture and locomotion. This thesis examines how forces are detected in the legs of cockroaches by tibial campaniform sensilla. Campaniform sensilla are mechanoreceptors that encode forces through ovoid cuticular caps embedded in the exoskeleton. The tibial sensilla are unique in that they consist of two subgroups with mutually perpendicular cap orientations.

We characterized the responses of the tibial receptors in restrained preparations by applying forces to the leg at controlled magnitudes and rates. The tibial sensilla, as a group, were sensitive to increasing and …

Use Of Oak Hammocks By Neotropical Migrant Songbirds During Stopover In Coastal South Carolina, Scott Glen Somershoe

Legacy ETDs

Many species of Neotropical migrant songbirds have been reported to be declining in the last few decades, and the loss and fragmentation of stopover habitat has been implicated as a potential cause of these population declines. To manage properly a fragmented forest landscape for migrating songbirds information is needed on how the size and the habitat of forest fragments influence their use by migrants during stopover. Therefore, I conducted censuses of Neotropical migrant birds in eight oak hammocks of varying size (0.32-3.08 ha) at the Savannah National Wildlife Refuge, Jasper County, South Carolina. Censuses were conducted at 0600-1030 h during …

Crystal Structure Of Bis(7-Nitro-8-Quinolinolato)Copper(Ii), Cuc18h10o6n4 / M. Shoja, H. Gershon And D. D. Clarke Fordham University, Department Of Chemistry, Fordham Road, Bronx, Ny 10458, Usa, Massud Shoja, Herman Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

C1sHwCuN406, monoclinic, P121/cl (No. 14), a= 4.567(9) Å, b = 10.723(8) Å, c = 17.095(6) Å, ~ = 100.0(1)0 , v = 824.5 Å3 , Z = 2, Rgt(F) = 0.043, wRgt(F) = 0.067, T = 293 K

Synthesis Of 2-Acetamido-5,6-Dihalophenyl Acetates / Donald D. Clarke, Herman Gershon, And John J. Mcmahon Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Donald Dudley Clarke Phd, Herman Gershon, John J. Mcmahon

Chemistry Faculty Publications

2-Acetamido-5,6-dihalophenyl acetates were synthesized as intermediates for the preparation of 6, 7-dihalo-8-quinolinols via the Skraup procedure

The Influence Of Leptin On Metabolic Expenditure And Thermogenesis During Thyroid Hormone (T₃) Suppression In The Obese (Ob/Ob) Mouse, Brian Kimball Underhill

Theses Digitization Project

No abstract provided.

Effect Of Thyroid Hormone On Formation Of The Nsf Fusion Complex In Normal And Obese Diabetic (Db/Db) Mice, Felipe Barajas Galicia

Theses Digitization Project

No abstract provided.

Typical Friedreich’S Ataxia Without Gaa Expansions And Gaa Epansions Wthout Typical Friedreich’S Ataxia, Dominick Mccabe, Fergus Ryan, D. Moore, Shirley Mcquaid, M. King, A. Kelly, K. Daly, David Barton, R. Murphy

Articles

We clinically assessed and performed polymerase chain reaction analysis for the GAA trinucleotide repeat expansion in 103 patients from 73 families in Ireland, with a prior clinical diagnosis of Friedreich’s ataxia (FA) or an unclassified progressive ataxic syndrome. The patients were classified as “typical” or “atypical” FA according to Harding’s mandatory clinical diagnostic criteria. All patients underwent blood glucose analysis, and electrocardiography and echocardiography was performed in 99 and 101 patients, respectively. Mutation screening for expanded CAG trinucleotide repeats, associated with spinocerebellar ataxia (SCA) 1, 2, 3 and 6 was performed in 86 patients overall, including all GAA negative patients. …

Absence Of Insect Juvenile Hormones In The American Dog Tick, Dermacentor Veriabilis (Say) (Acari: Ixodidae), And In Ornithodoros Parkeri Cooley (Acari: Argasidae), P. A. Neese, Daniel E. Sonenshine, V. L. Kallapur, C. S. Apperson, R. M. Roe

Biological Sciences Faculty Publications

Synganglia, salivary gland, midgut, ovary, fat body and muscle alone and in combination from the ixodid tick, Dermacentor variabilis (Say), or the argasid tick, Ornithodoros parkeri Cooley, were incubated in vitro in separate experiments with _L-[methyl-³H]methionine and farnesoic acid or with [1-¹⁴C]acetate. Life stages examined in D. variabilis were 3 and 72 h old (after ecdysis) unfed nymphs, partially fed nymphs (18 and 72 h after attachment to the host), fully engorged nymphs (2 d after detachment from host), 3 and 72 h old (after eclosion) unfed females, partially fed unmated females (12–168 …

Melanocortin-1-Receptor (Mcr-1) Gene Polymorphisms Associated With The Chicken E Locus Alleles, Andrew Ellett

Inquiry: The University of Arkansas Undergraduate Research Journal

The melanocortin 1-receptor (MCl-R) gene has been associated with E locus phenotypes in chickens. Variant alleles of the E locus are important for accurate down color sexing and also for the inhibition of unwanted tissue pigmentation in broilers. PCR (polymerase chain reaction) based tests for various replacement substitutions found in the published E allele sequences gave unexpected results when tested against known alleles of the E locus. To resolve these issues and gain a better understanding of how replacement substitutions in the MCl-R gene are affecting E locus phenotypes, a number of accessions of the e+, eb, wheaten ( ewh …