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Biochemistry, Biophysics, and Structural Biology
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
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Studies On The Roles Of Atp In Nitrogenase Catalysis, Wei Wu
Studies On The Roles Of Atp In Nitrogenase Catalysis, Wei Wu
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Nitrogenase is the enzyme that catalyzes the reduction of nitrogen to ammonia in a reaction requiring MgATP hydrolysis. Two component proteins of nitrogenase are the iron protein (Fe protein) and the molybdenum-iron protein (MoFe protein).
Nitrogenase contains two nucleotide binding sites. During catalysis, the Fe protein binds two MgATP first. The conformational changes induced upon MgATP binding allow the Fe protein to associate with the MoFe protein. After the formation of the Fe protein-MoFe protein complex, a single electron is transferred from the Fe protein to the MoFe protein, an event that is coupled to MgATP hydrolysis in the Fe …