Open Access. Powered by Scholars. Published by Universities.®
- File Type
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Use Of Biotin-Labeled Nucleic Acids For Protein Purification And Agarose-Based Chemiluminescent Electromobility Shift Assays., Joseph Rodgers, Pinky Patel, Jason Hennes, Sara Bolognia, David Mascotti
Use Of Biotin-Labeled Nucleic Acids For Protein Purification And Agarose-Based Chemiluminescent Electromobility Shift Assays., Joseph Rodgers, Pinky Patel, Jason Hennes, Sara Bolognia, David Mascotti
David P. Mascotti
We have employed biotin-labeled RNA to serve two functions. In one, the biotin tethers the RNA to streptavidin–agarose beads, creating an affinity resin for protein purification. In the other, the biotin functions as a label for use in a modified chemiluminescent electromobility shift assay (EMSA), a technique used to detect the formation of protein–RNA complexes. The EMSA that we describe avoids the use not only of radioactivity but also of neurotoxic acrylamide by using agarose as the gel matrix in which the free nucleic acid is separated from protein–nucleic acid complexes. After separation of free from complexed RNA in agarose, …
Dependence Of The Extent Of Ion Association With Polynucleotides And Peptides As A Function Of Solution Dielectric., David Mascotti, A. Salwan
Dependence Of The Extent Of Ion Association With Polynucleotides And Peptides As A Function Of Solution Dielectric., David Mascotti, A. Salwan
David P. Mascotti
No abstract provided.
Green Fluorescent Protein As A Quantitative Reporter Of Relative Promoter Activity In E-Coli., James Lissemore, J. Jankowski, C. Thomas, David Mascotti, P. Dehaseth
Green Fluorescent Protein As A Quantitative Reporter Of Relative Promoter Activity In E-Coli., James Lissemore, J. Jankowski, C. Thomas, David Mascotti, P. Dehaseth
David P. Mascotti
No abstract provided.