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Articles 1 - 30 of 428

Full-Text Articles in Biomaterials

Production And Biocompatibility Of Spider Silk Proteins In Goat Milk, Richard E. Decker Jr Dec 2018

Production And Biocompatibility Of Spider Silk Proteins In Goat Milk, Richard E. Decker Jr

All Graduate Theses and Dissertations

Due to its strength, flexibility, and biocompatibility, spider silk is a highly appealing material for applications in the medical field. Unfortunately, natural spider silk is difficult to obtain in large quantities because spiders are territorial and cannibalistic, making them impractical to farm. Synthetic spider silk proteins produced by transgenic hosts such as bacteria and goats have made it possible to obtain the quantities of spider silk needed to study it more fully and to investigate its potential uses. The spider silk proteins produced in our laboratory do not have an optimal purification method to remove all of the non-biocompatible contaminants ...


Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Darnell Leon Cuylear, Heather Elizabeth Canavan Nov 2018

Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Darnell Leon Cuylear, Heather Elizabeth Canavan

Shared Knowledge Conference

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the United States. The most reliable screening method of CRC is a colonoscopy which requires a 4-Liter polymer with electrolytes preparation. Two in five patients are non-compliant to their colonoscopy schedules, with many patients who abstain reporting refusal due to significant discomfort associated with this preparation. Furthermore, there are distinct gender differences in the tolerance of colonoscopy preparations in male and female populations. We hypothesize the differences in clinic are a result of toxic effects of the drug associated with poor mixing by individual patients. PEG, the drug ...


An Engineer’S Take On The Bone-Ligament Interface: Utilizing Novel Technology To Improve Clinical Outcomes, Emma Garcia Nov 2018

An Engineer’S Take On The Bone-Ligament Interface: Utilizing Novel Technology To Improve Clinical Outcomes, Emma Garcia

Shared Knowledge Conference

Ligament repair is a common surgical practice with a significant lack of viable replacements. The current gold standard for repair is the use of tendon grafts from cadavers or from another place in the patient’s body; however, these often cause more problems than they solve including immune responses or a lack of mobility in another place in the body. Synthetic replacements are of growing interest, though the ability to mimic the complex structure of the ligament and how it connects to the bone remains an obstacle. Our lab built a 3D bioprinter combined with an electrospinner to address this ...


3d Bioprinting And Near-Field Electrospinning Composite Scaffolds For The Bone-Ligament Interface, Emma Garcia, Christina Salas, Matthew N. Rush, Christopher Buksa, Marissa Perez, Ava Mauser, Steven Nery, Fermin Prieto, Darielys Morales Nov 2018

3d Bioprinting And Near-Field Electrospinning Composite Scaffolds For The Bone-Ligament Interface, Emma Garcia, Christina Salas, Matthew N. Rush, Christopher Buksa, Marissa Perez, Ava Mauser, Steven Nery, Fermin Prieto, Darielys Morales

Shared Knowledge Conference

3D bioprinting is an additive manufacturing technique that can utilize a range of bioactive materials to construct specific architectures that mimic native tissue. Near-field electrospinning (NFE) offers precise alignment control to create non-woven mats with high tensile strengths. We built a custom E-spin printer that enables layer-by-layer alternating deposition between 3D bioprinting and NFE to create composite scaffolds for the bone-ligament interface. This complex region is difficult to simulate due to its functionally graded mechanical and biochemical properties. We created NFE poly(caprolactone) highly aligned micro-fibers which formed collagen fibril-like bundles. Poly(ethylene glycol) diacrylate with decellularized bone was encased ...


Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Sarah Mounho, Darnell Cuylear, Heather Canavan Nov 2018

Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Sarah Mounho, Darnell Cuylear, Heather Canavan

Shared Knowledge Conference

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the United States. The most reliable screening method of CRC is a colonoscopy which requires a 4-Liter polymer with electrolytes preparation. Two in five patients are non-compliant to their colonoscopy schedules, with many patients who abstain reporting refusal due to significant discomfort associated with this preparation. Furthermore, there are distinct gender differences in the tolerance of colonoscopy preparations in male and female populations. We hypothesize the differences in clinic are a result of toxic effects of the drug associated with poor mixing by individual patients. PEG, the drug ...


Development Of Granulation Tissue Mimetic Scaffolds For Skin Healing, Adam Hopfgartner Oct 2018

Development Of Granulation Tissue Mimetic Scaffolds For Skin Healing, Adam Hopfgartner

Electronic Thesis and Dissertation Repository

Impaired skin healing is a significant and growing clinical concern, particularly in relation to diabetes, venous insufficiency and immobility. Previously, we developed electrospun scaffolds for the delivery of periostin (POSTN) and connective tissue growth factor 2 (CCN2), matricellular proteins involved in the proliferative phase of healing. This study aimed to design and validate a novel electrosprayed coaxial microsphere for the encapsulation of fibroblast growth factor 9 (FGF9), as a component of the POSTN/CCN2 scaffold, to promote angiogenic stability during wound healing. For the first time, we observed a pro-proliferative effect of FGF9 on human dermal fibroblasts (HDF) in vitro ...


Pneumatospinning Of Collagen Microfibers From Benign Solvents, Seth Polk, Nardos Sori, Nick Thayer, Yas Maghdouri-White, Anna A. Bulysheva, Michael P. Francis Oct 2018

Pneumatospinning Of Collagen Microfibers From Benign Solvents, Seth Polk, Nardos Sori, Nick Thayer, Yas Maghdouri-White, Anna A. Bulysheva, Michael P. Francis

Medical Diagnostics & Translational Sciences Faculty Publications

Introduction. Current collagen fiber manufacturing methods for biomedical applications, such as electrospinning and extrusion, have had limited success in clinical translation, partially due to scalability, cost, and complexity challenges. Here we explore an alternative, simplified and scalable collagen fiber formation method, termed 'pneumatospinning,' to generate submicron collagen fibers from benign solvents. Methods and results. Clinical grade type I atelocollagen from calf corium was electrospun or pneumatospun as sheets of aligned and isotropic fibrous scaffolds. Following crosslinking with genipin, the collagen scaffolds were stable in media for over a month. Pneumatospun collagen samples were characterized using Fourier-transform infrared spectroscopy, circular dichroism ...


Electrospun Collagen Fibers For Tissue Regeneration Applications, Ying Li Sep 2018

Electrospun Collagen Fibers For Tissue Regeneration Applications, Ying Li

Electronic Thesis and Dissertation Repository

Tissue engineering aims to regenerate damaged and deceased tissue by combining cells with scaffold made from an appropriate biomaterial and providing a conducive environment to guide cell growth and the formation or regeneration of new tissue or organ. While collagen, an important material of the extracellular matrix (ECM), is a natural choice as a scaffold biomaterial, the conducive environment can only be created by having the ability to control the geometry, organization, structural and mechanical properties of the scaffold. Moreover, degradability and degradation rate control of the scaffold has to be taken into consideration too. In this work, we aim ...


Fabrication And Evaluation Of Poly(Lactic Acid), Chitosan, And Tricalcium Phosphate Biocomposites For Guided Bone Regeneration, Srikanthan Ramesh, Lisa Lungaro, Dimitrios Tsikritsis, Eric Weflen, Iris V. Rivero Aug 2018

Fabrication And Evaluation Of Poly(Lactic Acid), Chitosan, And Tricalcium Phosphate Biocomposites For Guided Bone Regeneration, Srikanthan Ramesh, Lisa Lungaro, Dimitrios Tsikritsis, Eric Weflen, Iris V. Rivero

Industrial and Manufacturing Systems Engineering Publications

This study presents and evaluates an approach for fabricating poly(lactic acid) (PLA)/chitosan (CS)/tricalcium phosphate (TCP) electrospun scaffolds for guided bone regeneration, a dental procedure that uses membranes to direct and delineate regions of osteogenesis. Biomaterials were pre‐processed using cryomilling, a solid‐state grinding technique that facilitates the generation of powdered biocomposites conducive to electrospinning. X‐ray diffraction (XRD) confirmed the generation of cryomilled blends consisting of PLA, CS, and TCP. Results from the differential scanning calorimetry showed an upward shift in glass transition temperature and an increase in crystallinity with the inclusion of TCP reinforcing the ...


Developing Strategies To Toughen Bio-Inspired Adhesives, Narelli P. Narciso, Samuel Lee Huntington, Jonathan J. Wilker Aug 2018

Developing Strategies To Toughen Bio-Inspired Adhesives, Narelli P. Narciso, Samuel Lee Huntington, Jonathan J. Wilker

The Summer Undergraduate Research Fellowship (SURF) Symposium

Mussels and other marine creatures adhere very well in underwater environments, having the ability to withstand the force of the sea. These animals have inspired synthetic biomimetic adhesives for wet systems, presenting potential for biomedical applications. However, most current commercial adhesives tend to be brittle, not resisting repetitive movements. This study assesses toughening strategies to improve the mussel-inspired adhesives’ ductility while maintaining its strength. The strategies included altering the polymer’s chemical structure by changing the percentage of polyethylene glycol (PEG) in the molecule and by adding fillers, such as calcium carbonate, silica and nacre - a calcium carbonate compound found ...


Piezoresponse, Mechanical, And Electrical Characteristics Of Synthetic Spider Silk Nanofibers, Nader Shehata, Ishac Kandas, Ibrahim Hassounah, Patrik Sobolčiak, Igor Krupa, Miroslav Mrlik, Anton Popelka, Jesse Steadman, Randolph Lewis Aug 2018

Piezoresponse, Mechanical, And Electrical Characteristics Of Synthetic Spider Silk Nanofibers, Nader Shehata, Ishac Kandas, Ibrahim Hassounah, Patrik Sobolčiak, Igor Krupa, Miroslav Mrlik, Anton Popelka, Jesse Steadman, Randolph Lewis

Biology Faculty Publications

This work presents electrospun nanofibers from synthetic spider silk protein, and their application as both a mechanical vibration and humidity sensor. Spider silk solution was synthesized from minor ampullate silk protein (MaSp) and then electrospun into nanofibers with a mean diameter of less than 100 nm. Then, mechanical vibrations were detected through piezoelectric characteristics analysis using a piezo force microscope and a dynamic mechanical analyzer with a voltage probe. The piezoelectric coefficient (d33) was determined to be 3.62 pC/N. During humidity sensing, both mechanical and electric resistance properties of spider silk nanofibers were evaluated at varying high-level ...


Engineering Graphene Oxide-Based Nanostructures For Dna Sensors, Aditya Balaji Jul 2018

Engineering Graphene Oxide-Based Nanostructures For Dna Sensors, Aditya Balaji

Electronic Thesis and Dissertation Repository

Various nanostructures have been explored in DNA biosensors to convert the hybridization of DNA sequences to easily measurable processes, including optical, mechanical, magnetic, or electrochemical process. In this thesis, graphene oxide, a two-dimensional nanostructure, is applied in quenching the fluorescence of a core-shell nanoparticles modified with targeted DNA sequences. The core-shell nanoparticles, iron oxide (Fe3O4) core, and fluorescent silica (SiO2) shell, were produced through a wet chemical process which can directly link to a targeted DNA sequence (DNA-t), and the graphene oxide nanosheets were produced by the oxidation of graphite. In the meantime, a complementary- DNA ...


Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline A. De Lora Jul 2018

Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline A. De Lora

Biomedical Sciences ETDs

Adaptation of cancer cells to changes in the biochemical microenvironment in an expanding tumor mass is a crucial aspect of malignant progression, tumor metabolism, and drug efficacy. In vitro, it is challenging to mimic the evolution of biochemical gradients and the cellular heterogeneity that characterizes cancer tissues found in vivo. It is well accepted that more realistic and controllable in vitro 3D model systems are required to improve the overall cancer research paradigm and thus improve on the translation of results, but multidisciplinary approaches are needed for these advances. This work develops such approaches and demonstrates that new droplet-based cell-encapsulation ...


Preparation And Characterization Of Electrospun Rgo-Poly(Ester Amide) Tissue Engineering Scaffolds, Hilary Stone Jun 2018

Preparation And Characterization Of Electrospun Rgo-Poly(Ester Amide) Tissue Engineering Scaffolds, Hilary Stone

Electronic Thesis and Dissertation Repository

Tissue engineering scaffolds should support tissue maturation through exposure to biologically relevant stimuli and through successful cell infiltration. External electrical stimulation is particularly relevant for cardiac and neural applications, and requires conductive scaffolds to propagate electrical signals; cell infiltration is only possible with scaffolds that have sufficient porosity. The aim of this study was to impart conductivity and increased porosity of electrospun poly(ester amide) (PEA) scaffolds. Reduced graphene oxide (rGO) was incorporated into blend PEA and coaxial PEA-chitosan fibrous scaffolds, which increased scaffold conductivity and supported cardiac differentiation. The novel combination of ultrasonication and leaching of a sacrificial polymer ...


Soft-Microrobotics: The Manipulation Of Alginate Artificial Cells, Samuel Sheckman May 2018

Soft-Microrobotics: The Manipulation Of Alginate Artificial Cells, Samuel Sheckman

Mechanical Engineering Research Theses and Dissertations

In this work, the approach to the manipulation of alginate artificial cell soft-microrobots, both individually and in swarms is shown. Fabrication of these artificial cells were completed through centrifugation, producing large volumes of artificial cells, encapsulated with superparamagnetic iron oxide nanoparticles; these artificial cells can be then externally stimulated by an applied magnetic field. The construction of a Permeant Magnet Stage (PMS) was produced to manipulate the artificial cells individually and in swarms. The stage functionalizes the permanent magnet in the 2D xy-plane. Once the PMS was completed, Parallel self-assembly (Object Particle Computation) using swarms of artificial cells in complex ...


Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch May 2018

Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch

University Scholar Projects

Recent advances in the field of biomaterials have suggested that cells cultured on substrates resembling the native tissue mechanical properties, matrix and growth factor composition, and topography can adopt phenotypes that more closely resemble the in vivo tissue compared to cells cultured on non-mimetic constructs. Understanding the effect of culture substrate on in vitro tissue formation is important for bioengineering applications that include mechanistic studies of healthy tissue function and development of disease models. In this work, Caco-2 adenocarcinoma cells were seeded on flat and crypt-like topographies of 3D-printed cytocompatible hydrogels derived from silk fibroin protein. Silk hydrogels were selected ...


Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch May 2018

Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch

Honors Scholar Theses

Recent advances in the field of biomaterials have suggested that cells cultured on substrates resembling the native tissue mechanical properties, matrix and growth factor composition, and topography can adopt phenotypes that more closely resemble the in vivo tissue compared to cells cultured on non-mimetic constructs. Understanding the effect of culture substrate on in vitro tissue formation is important for bioengineering applications that include mechanistic studies of healthy tissue function and development of disease models. In this work, Caco-2 adenocarcinoma cells were seeded on flat and crypt-like topographies of 3D-printed cytocompatible hydrogels derived from silk fibroin protein. Silk hydrogels were selected ...


Designing Synthetic Environments To Control Valvular Interstital Cells In Vitro, Kent E. Coombs May 2018

Designing Synthetic Environments To Control Valvular Interstital Cells In Vitro, Kent E. Coombs

Biomedical Sciences ETDs

Aortic valve disease (AVD) is a large contributor to health costs in the United States affecting 2.8% of the population greater than 75 years old. With a growing elderly population due to medical advances, AVD will continue to rise in prevalence over time. Current treatments for AVD are insufficient due to a lack of preventative therapies and the bioprosthetic valves used for surgical replacement have major limitations. Tissue engineered heart valves (TEHVs) present an ideal solution to current AVD needs because of their biocompatibility, capability to integrate with the host’s tissue, and ability to utilize the natural repair ...


Plga-Modified Nanoparticles For The Treatment Of Hypo-Vascularized Hpv-Related Cervical Cancers., Lee B. Sims May 2018

Plga-Modified Nanoparticles For The Treatment Of Hypo-Vascularized Hpv-Related Cervical Cancers., Lee B. Sims

Electronic Theses and Dissertations

A major challenge associated with delivery of active agents in the female reproductive tract (FRT) is the ability of agents to efficiently diffuse through the cervicovaginal mucosa (CVM) and reach the underlying sub-epithelial immune cell layer and vasculature. A variety of drug delivery vehicles have been employed to improve the delivery of agents across the CVM and offer the capability to increase the longevity and retention of active agents to treat infections of the female reproductive tract. Nanoparticles (NPs) have been shown to improve retention, diffusion, and cell-specific targeting via specific surface modifications, relative to other delivery platforms. In particular ...


Efficient Blue To Red Afterglow Tuning In A Binary Nanocomposite Plastic Film, Yan Xia, Huase Ou, Wanbin Li, Gang Han, Zhanjun Li Apr 2018

Efficient Blue To Red Afterglow Tuning In A Binary Nanocomposite Plastic Film, Yan Xia, Huase Ou, Wanbin Li, Gang Han, Zhanjun Li

Open Access Articles

Colorful spectra are important for the diverse applications of persistent phosphors. A color conversion concept is developed to obtain abundant persistent luminescence color by mining capacities of known persistent phosphors with the most efficient persistent properties. Here, SiO(2)/Sr(2)MgSi(2)O(7):Eu,Dy nanoparticles are chosen as a blue persistent luminescence donor nanophosphor, while ultrafine CaAlSiN(3):Eu is utilized as a red conversion phosphor to tune the persistent luminescence spectra from blue to red. The red afterglow emission can persist for more than 5 h. The decay of the red afterglow follows nearly the same ...


Materials Design With Polylactic Acid-Polyethylene Glycol Blends Using 3d Printing And For Medical Applications., Jeremiah R. Bauer Apr 2018

Materials Design With Polylactic Acid-Polyethylene Glycol Blends Using 3d Printing And For Medical Applications., Jeremiah R. Bauer

Electronic Theses and Dissertations

This thesis is an examination of two material systems derived from polylactic acid (PLA) and polyethylene glycol (PEG). PLA is a polymer commonly sourced from renewable sources such as starches and sugars. It is a relatively strong, biodegradable polymer, making it ideal for use in the body. Even though it has a relative high strength, PLA is also brittle leading to the use of plasticizers to increase flexibility. One such plasticizer is PEG, which is a material that can exist at room temperature as either a thin liquid, or a hard waxy solid depending on the molecular weight. The first ...


Osteon Mimetic Scaffolding, Janay Clytus Apr 2018

Osteon Mimetic Scaffolding, Janay Clytus

Senior Theses

The purpose of this research is to provide an alternative to naturally derived bone grafts. There is a gap in the supply of donors and the demand of bone tissue. Artificial scaffold creation can work as an implant and decrease the shortage of bone grafts and increase the range of injuries that can be repaired. Current research focuses on optimizing mechanical properties such as porosity, improving vascularization using cells, and generating osteoconductivity. For osteodifferentiation, mesenchymal stem cells (MSCs) can differentiate into mesodermal lineages such as chondrocytes, osteoblasts, adipocytes, and tenocytes by supplementing cultures with lineage-specific soluble factors (Marchetti). Co-culturing ECFCs ...


Recent Approaches In Designing Bioadhesive Materials Inspired By Mussel Adhesive Protein, Pegah Kord Forooshani, Bruce P. Lee Mar 2018

Recent Approaches In Designing Bioadhesive Materials Inspired By Mussel Adhesive Protein, Pegah Kord Forooshani, Bruce P. Lee

Bruce Lee

Marine mussels secret protein-based adhesives, which enable them to anchor to various surfaces in a saline, intertidal zone. Mussel foot proteins (Mfps) contain a large abundance of a unique, catecholic amino acid, Dopa, in their protein sequences. Catechol offers robust and durable adhe-sion to various substrate surfaces and contributes to the curing of the adhesive plaques. In this article, we review the unique features and the key functionalities of Mfps, catechol chemistry, and strategies for preparing catechol-functionalized poly- mers. Specifically, we reviewed recent findings on the contributions of various features of Mfps on interfacial binding, which include coacervate formation, surface ...


Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal Feb 2018

Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal

Electronic Thesis and Dissertation Repository

Scaffolds are key components for bone tissue engineering and regeneration. They guide new bone formation by mimicking bone extracellular matrix for cell recruitment and proliferation. Ideally, scaffolds for bone tissue engineering need to be osteoconductive, osteoinductive, porous, degradable and mechanically competent. As a single material can not provide all these requirements, composites of several biomaterials are viable solutions to combine various properties. However, conventional composites fail to fulfil these requirements due to their distinct phases at the microscopic level. Organic/inorganic (O/I) class II hybrid biomaterials, where the organic and inorganic phases are chemically crosslinked on a molecular scale ...


Collective Adhesion And Displacement Of Retinal Progenitor Cells Upon Extracellular Matrix Substrates Of Transplantable Biomaterials, Ankush Thakur, Shawn Mishra, Juan Pena, Jing Zhou, Stephen Redenti, Robert Majeska, Maribel Vazquez Jan 2018

Collective Adhesion And Displacement Of Retinal Progenitor Cells Upon Extracellular Matrix Substrates Of Transplantable Biomaterials, Ankush Thakur, Shawn Mishra, Juan Pena, Jing Zhou, Stephen Redenti, Robert Majeska, Maribel Vazquez

Publications and Research

Strategies to replace retinal photoreceptors lost to damage or disease rely upon the migration of replacement cells transplanted into sub-retinal spaces. A significant obstacle to the advancement of cell transplantation for retinal repair is the limited migration of transplanted cells into host retina. In this work, we examine the adhesion and displacement responses of retinal progenitor cells on extracellular matrix substrates found in retina as well as widely used in the design and preparation of transplantable scaffolds. The data illustrate that retinal progenitor cells exhibit unique adhesive and displacement dynamics in response to poly-l-lysine, fibronectin, laminin, hyaluronic acid, and Matrigel ...


Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund Jan 2018

Polymeric Peptide Mimics For Protein Delivery, Coralie Backlund

Doctoral Dissertations

The plasma membrane is a major obstacle in the development and use of biomacromolecules for intracellular applications. Consequently, proteins with intracellular targets represent an enormous, yet under studied avenue for therapeutics. Extended research has aimed at facilitating intracellular delivery of exogenous proteins using protein transduction domains (PTDs), which allow transport of bioactive molecules into cells. Synthetic polymers, inspired by PTDs, provide a well-controlled platform to vary molecular architecture for structure activity relationship studies. Specifically, this thesis focuses on the use of ring-opening metathesis, a facile and efficient polymerization technique, through which we can vary structural parameters to optimize delivery of ...


An Injectable Thermosensitive Biodegradable Hydrogel Embedded With Snap Containing Plla Microparticles For Sustained Nitric Oxide (No) Delivery For Wound Healing, Nikhil Mittal Jan 2018

An Injectable Thermosensitive Biodegradable Hydrogel Embedded With Snap Containing Plla Microparticles For Sustained Nitric Oxide (No) Delivery For Wound Healing, Nikhil Mittal

Dissertations, Master's Theses and Master's Reports

After injury, wound healing is a complex sequential cascade of events essential for the proper recovery of the wound without the scar formation. Nitric oxide (NO) is a small, endogenous free-radical gas with antimicrobial, vasodilating and growth factor stimulating properties. NO has wide biomedical application especially in wound healing however, its usability is hindered due its administration problem as it is highly unstable.

In this work, poly (l-lactic acid) microparticles encapsulated with NO donor S-nitroso-N-acetyl-D-penicillamine (SNAP) were prepared using water-in-oil-water double emulsion solvent evaporation method for controlled delivery for NO at the specific site. The NO release from SNAP-PLLA microparticles ...


Electrochemical And Spectroscopic Study Of Peptide-Template Melanins, Daniel Courter, Olivia Rossi, Zhen Tian Jan 2018

Electrochemical And Spectroscopic Study Of Peptide-Template Melanins, Daniel Courter, Olivia Rossi, Zhen Tian

Honors Theses and Capstones

Melanins are organic polymers made up of 5,6-dihydroxyindole carboxylic acid (DHICA) and 5,6-dihydroxyindole (DHI). Biochemical systems synthesize melanins through a complex reaction pathway. Previous research exhibited that naturally-occurring melanins (NatMel) and their synthetic analogues (SynMel) can potentially serve as electrode materials for aqueous charge storage devices. Small surface area creates technical challenges for these melanins as charge storage materials. This research herein demonstrates that the structure of melanins can affect the charge storage capacities of monovalent Na+cations and divalent Mg2+ cations. 2-D sheet-like peptide melanins (PepMel) were synthesized into tripeptides consisting of aspartic acid (D), phenylalanine ...


Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi Jan 2018

Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi

Undergraduate Honors Theses

Heart disease continues to be the leading cause of death in both men and women. It is accompanied with cellular- and organ- level remodeling that cannot be mimicked or recaptured by cells on standard culture platforms. Polyethylene glycol hydrogels bridge this gap by providing user-defined tunability for cell culture. A novel 3D cell culture system was developed to encapsulate neonatal rat ventricular cardiomyocytes (NRVMs) in a single plane, and cell health was assessed. In parallel, recombinant adenoviruses containing the mutations responsible for human hypertrophic cardiomyopathy (HCM) in the beta myosin gene (R403Q and R453C) were generated and used as a ...


Studying Mass And Mechanical Property Changes During The Degradation Of A Bioadhesive With Mass Tracking, Rheology And Magnetoelastic (Me) Sensors, Zhongtian Zhang Jan 2018

Studying Mass And Mechanical Property Changes During The Degradation Of A Bioadhesive With Mass Tracking, Rheology And Magnetoelastic (Me) Sensors, Zhongtian Zhang

Dissertations, Master's Theses and Master's Reports

In this research, the degradable polymer 4-arm poly (ethylene glycol)-glutaric acid-dopamine (PEG-GA-DM4) was synthesized. The degradation behavior of crosslinked PEG-GA-DM4 bioadhesive was studied with mass tracking, oscillatory rheology, and magnetoelastic (ME) sensors. Changes in mechanical properties were correlated with both dry mass and wet mass changes during the degradation. The results indicate that the loss of mechanical property in the bioadhesive can take place without losing the dry mass. The mass loss profile cannot describe the degradation behavior completely. In addition to studying the degradation of PEG-GA-DM4, this research also confirms the application of ME sensors ...