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Electrospinning

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Full-Text Articles in Biomedical Engineering and Bioengineering

Electrospun Collagen Fibers For Tissue Regeneration Applications, Ying Li Sep 2018

Electrospun Collagen Fibers For Tissue Regeneration Applications, Ying Li

Electronic Thesis and Dissertation Repository

Tissue engineering aims to regenerate damaged and deceased tissue by combining cells with scaffold made from an appropriate biomaterial and providing a conducive environment to guide cell growth and the formation or regeneration of new tissue or organ. While collagen, an important material of the extracellular matrix (ECM), is a natural choice as a scaffold biomaterial, the conducive environment can only be created by having the ability to control the geometry, organization, structural and mechanical properties of the scaffold. Moreover, degradability and degradation rate control of the scaffold has to be taken into consideration too. In this work, we aim ...


Preparation And Characterization Of Electrospun Rgo-Poly(Ester Amide) Tissue Engineering Scaffolds, Hilary Stone Jun 2018

Preparation And Characterization Of Electrospun Rgo-Poly(Ester Amide) Tissue Engineering Scaffolds, Hilary Stone

Electronic Thesis and Dissertation Repository

Tissue engineering scaffolds should support tissue maturation through exposure to biologically relevant stimuli and through successful cell infiltration. External electrical stimulation is particularly relevant for cardiac and neural applications, and requires conductive scaffolds to propagate electrical signals; cell infiltration is only possible with scaffolds that have sufficient porosity. The aim of this study was to impart conductivity and increased porosity of electrospun poly(ester amide) (PEA) scaffolds. Reduced graphene oxide (rGO) was incorporated into blend PEA and coaxial PEA-chitosan fibrous scaffolds, which increased scaffold conductivity and supported cardiac differentiation. The novel combination of ultrasonication and leaching of a sacrificial polymer ...


Identifying And Reducing Variability, Improving Scaffold Morphology, And Investigating Alternative Materials For The Blood Vessel Mimic Lab Electrospinning Process, Evan M. Dowey Sep 2017

Identifying And Reducing Variability, Improving Scaffold Morphology, And Investigating Alternative Materials For The Blood Vessel Mimic Lab Electrospinning Process, Evan M. Dowey

Master's Theses and Project Reports

The work of the Cal Poly Tissue Engineering Lab is primarily focused on the fabrication, characterization, and improvement of “Blood Vessel Mimics” (BVMs), tissue engineered constructs used to evaluate cellular response to vascular medical devices. Currently, cells are grown onto fibrous, porous tubes made using an in-house electrospinning process from PLGA, a biocompatible co-polymer. The adhesion and proliferation of cells in a BVM is reliant on the micro-scale structure of the PLGA scaffold, and as such it is of great importance for the electrospinning process to consistently produce scaffolds of similar morphologies. Additionally, it has been shown that cell proliferation ...


Modulating The Innate Immune Response To Electrospun Scaffolds And Polymer Degradative Byproducts, Daniel Abebayehu Jan 2017

Modulating The Innate Immune Response To Electrospun Scaffolds And Polymer Degradative Byproducts, Daniel Abebayehu

Theses and Dissertations

Implanted biomaterials often induce inflammation that frequently leads to the foreign body response, fibrosis, and the failure of the implant. Thus, it is important to evaluate how cells interact with materials to promote a more regenerative response. It is critical to determine how to modulate the response of tissue resident innate immune cells, as they are among the first cells to interact with implanted materials. Among tissue resident innate immune cells are mast cells, which are inflammatory sentinels that degranulate and orchestrate the fate of other cell populations, such as monocytes/macrophages and lymphocytes. Mast cells have also been reported ...


Development Of An Electrospun And 3d Printed Cellular Delivery Device For Dermal Wound Healing, Ryan M. Clohessy Jan 2017

Development Of An Electrospun And 3d Printed Cellular Delivery Device For Dermal Wound Healing, Ryan M. Clohessy

Theses and Dissertations

The goal of this research was to develop a system of individualized medicine that could be applied to dermal wounds serving as a wound dressing and synthetic extracellular matrix while delivering stem cells to the wound bed. First, fabrication parameters for electrospinning polymer fibers were determined. This involved evaluating fiber morphology with respect to polymer selection and solution concentration. Next, construct fabrication was examined to produce an integrated void space, or cargo area, suitable to maintain stem cells. In vitro studies to ensure stem cell viability and phenotype were conducted, and results supported the notion that cells could be administered ...


Development Of A Programmed Electrospun Three Dimensional (3d) Nanofiber Collecter And It’S Application To Orthopedic Implant Coatings, Liang Chen Jan 2017

Development Of A Programmed Electrospun Three Dimensional (3d) Nanofiber Collecter And It’S Application To Orthopedic Implant Coatings, Liang Chen

Wayne State University Dissertations

Orthopedic implants might not directly unite with bones especially in compromised patients even if they have been appropriately fixed. The lack of early osseointegration would lead to the failure of the orthopedic implant. A “bone-like” implant surface is urgently needed to accelerate osseointegration. Electrospun nanofiber (NF) is a promising implant coating due to its highly porous nanoscale structure. It mimics the collagen I nanofibrous network of bone tissue; meanwhile it has been widely used as a drug delivery device. However, its compact and dense structure is not ideal for cell growth. Our strategy was to develop a functional three-dimensional (3D ...


A 3d Biomimetic Scaffold Using Electrospinning For Tissue Engineering Applications, Samerender Nagam Hanumantharao Jan 2017

A 3d Biomimetic Scaffold Using Electrospinning For Tissue Engineering Applications, Samerender Nagam Hanumantharao

Dissertations, Master's Theses and Master's Reports

Electrospinning holds great promise for designing functional 3D biomimetic scaffolds for tissue engineering applications. The technique allows for the reproducible fabrication of 3D scaffolds with control over the porosity and thickness. In this work, a novel method for the synthesis of a 3D electroactive scaffold using electrospinning from polycaprolactone (PCL), Polyvinylidene Fluoride (PVDF) and Polyaniline (PANI) is reported. Additional scaffolds involving different morphologies of PCL, PCL-PVDF and PCL-PANI-PVDF were also fabricated and evaluated. The scaffolds were characterized using electron microscopy to visualize the morphologies. Infrared spectroscopy was used to confirm the presence of polymers and their respective phases in the ...


Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros Oct 2016

Electrospinning Of Poly (Ester Amide) Fibres For Mesenchymal Progenitor Cell Differentiation, Sarah Kiros

Electronic Thesis and Dissertation Repository

The in vitro vascular tissue engineering paradigm seeks to produce biologically responsive vascular substitutes using cells, biodegradable scaffolds, and bioreactors to mature the tissue for the potential treatment of vascular occlusions and to create 3D tissue models for pre-clinical testing. In this work, a poly (ester amide) (PEA) derived from from L-phenylalanine, sebacoyl chloride and 1,4 butanediol was synthesized and electrospun to form both 3D fibrous mats and tubular constructs. Both the polymer solution concentration and mandrel rotation speed were optimized to fabricate bead-free fibres. Cytocompatibility and proliferation studies using mesenchymal progenitor 10T1/2 cells showed PEA fibres were ...


Molecular Combing Of Dna Nanofibers And Comparison To Electrospinning, Tanner L. Buresh Apr 2016

Molecular Combing Of Dna Nanofibers And Comparison To Electrospinning, Tanner L. Buresh

UCARE Research Products

The goal of these experiments was to create DNA nanofibers through the method of molecular combing (MC) and then compare the MC fibers to fibers created with electrospinning (ES). The experiment was designed and initial samples were created. After confirming that the method would succeed in creating DNA fibers, several parametric studies were performed in order to optimize the experiment and create the most uniform fibers possible. The parametric studies were done on the following variables: substrate material, pH level of DNA solution, and DNA solution concentration. After completion of all experiments, it was determined that although fibers could be ...


Novel Small Airway Model Using Electrospun Decellularized Lung Extracellular Matrix, Bethany M. Young Jan 2016

Novel Small Airway Model Using Electrospun Decellularized Lung Extracellular Matrix, Bethany M. Young

Theses and Dissertations

Chronic respiratory diseases affects many people worldwide with little known about the mechanisms diving the pathology, making it difficult to find a cure. Improving the understanding of smooth muscle and extracellular matrix (ECM) interaction is key to developing a remedy to this leading cause of death. With currently no relevant or controllable in vivo or in vitro model to investigate diseased and normal interactions of small airway components, the development of a physiologically relevant in vitro model with comparable cell attachment, signaling, and organization is necessary to develop new treatments for airway disease. The goal of this study is to ...


Biopolymer Electrospun Nanofiber Mats To Inactivate And Remove Bacteria, Katrina Ann Rieger Jan 2016

Biopolymer Electrospun Nanofiber Mats To Inactivate And Remove Bacteria, Katrina Ann Rieger

Doctoral Dissertations

The persistence of antibiotic resistance in bacterial pathogens remains a primary concern for immunocompromised and critically-ill hospital patients. Hospital associated infections can be deadly and reduce the successes of medical advancements, such as, cancer therapies and medical implants. Thus, it is imperative to develop materials that can (i) deliver new antibiotics with accuracy, as well as (ii) uptake pathogenic microbes. In this work, we will demonstrate that electrospun nanofiber mats offer a promising platform for both of these objectives because of their high surface-to-volume ratio, interconnected high porosity, gas permeability, and ability to contour to virtually any surface. To provide ...


Modeling Early Stage Bone Regeneration With Biomimetic Electrospun Fibrinogen Nanofibers And Adipose-Derived Mesenchymal Stem Cells, Michael P. Francis, Yas M. Moghaddam-White, Patrick C. Sachs, Matthew J. Beckman, Stephen M. Chen, Gary L. Bowlin, Lynne W. Elmore, Shawn E. Holt Jan 2016

Modeling Early Stage Bone Regeneration With Biomimetic Electrospun Fibrinogen Nanofibers And Adipose-Derived Mesenchymal Stem Cells, Michael P. Francis, Yas M. Moghaddam-White, Patrick C. Sachs, Matthew J. Beckman, Stephen M. Chen, Gary L. Bowlin, Lynne W. Elmore, Shawn E. Holt

Medical Diagnostics & Translational Sciences Faculty Publications

The key events of the earliest stages of bone regeneration have been described in vivo although not yet modeled in an in vitro environment, where mechanistic cell-matrix-growth factor interactions can be more effectively studied. Here, we explore an early-stage bone regeneration model where the ability of electrospun fibrinogen (Fg) nanofibers to regulate osteoblastogenesis between distinct mesenchymal stem cells populations is assessed. Electrospun scaffolds of Fg, polydioxanone (PDO), and a Fg:PDO blend were seeded with adipose-derived mesenchymal stem cells (ASCs) and grown for 7-21 days in osteogenic differentiation media or control growth media. Scaffolds were analyzed weekly for histologic and ...


Fiber Based Approaches As Medicine Delivery Systems, Farrokh Sharifi, Avinash C. Sooriyarachchi, Hayriye Altural, Reza Montazami, Marissa Nichole Rylander, Nicole Nastaran Hashemi Jan 2016

Fiber Based Approaches As Medicine Delivery Systems, Farrokh Sharifi, Avinash C. Sooriyarachchi, Hayriye Altural, Reza Montazami, Marissa Nichole Rylander, Nicole Nastaran Hashemi

Mechanical Engineering Publications

The goal of drug delivery is to ensure that therapeutic molecules reach the intended target organ or tissue, such that the effectiveness of the drug is maximized. The efficiency of a drug delivery system greatly depends on the choice of drug carrier. Recently, there has been growing interest in using micro- and nanofibers for this purpose. The reasons for this growing interest include these materials’ high surface area to volume ratios, ease of fabrication, high mechanical properties, and desirable drug release profile. Here, we review developments in using these materials made by the most prevalent methods of fiber fabrication: electrospinning ...


Electrospinning Of Polycaprolactone Core-Shell Nanofibers With Anti-Cancer Drug, Sakib Iqbal, Mujibur Khan, Saheem Absar, Andrew Diamanduros, Samuel Chambers Apr 2015

Electrospinning Of Polycaprolactone Core-Shell Nanofibers With Anti-Cancer Drug, Sakib Iqbal, Mujibur Khan, Saheem Absar, Andrew Diamanduros, Samuel Chambers

Georgia Southern University Research Symposium

Encapsulation of a model anti-cancer drug, 5-Fluorouracul (5-FU) into biocompatible core-shell nanofibers of polycaprolactone (PCL) nanofibers was fabricated using a coaxial electrospinning process. Our work aims to solve these issues using a novel method of fabrication of fibers featuring confinement of drugs within a biodegradable core-shell structure, thereby permitting sustained release of drugs to specific sites of treatment, such as tissues affected with tumor cells. The coaxial electrospinning was performed using a sheath polymer solution consisting of a 14 wt% PCL solution and a 5 wt% solution of 5-FU as the core solution. Dimethylformamide (DMF) was used as the solvent ...


Fiber Scaffolds Of Poly (Glycerol-Dodecanedioate) And Its Derivative Via Electrospinning For Neural Tissue Engineering, Xizi Dai Mar 2015

Fiber Scaffolds Of Poly (Glycerol-Dodecanedioate) And Its Derivative Via Electrospinning For Neural Tissue Engineering, Xizi Dai

FIU Electronic Theses and Dissertations

Peripheral nerves have demonstrated the ability to bridge gaps of up to 6 mm. Peripheral Nerve System injury sites beyond this range need autograft or allograft surgery. Central Nerve System cells do not allow spontaneous regeneration due to the intrinsic environmental inhibition. Although stem cell therapy seems to be a promising approach towards nerve repair, it is essential to use the distinct three-dimensional architecture of a cell scaffold with proper biomolecule embedding in order to ensure that the local environment can be controlled well enough for growth and survival. Many approaches have been developed for the fabrication of 3D scaffolds ...


Peracetic Acid: A Practical Agent For Sterilizing Heat-Labile Polymeric Tissue-Engineering Scaffolds, William R. Trahan Jan 2015

Peracetic Acid: A Practical Agent For Sterilizing Heat-Labile Polymeric Tissue-Engineering Scaffolds, William R. Trahan

Theses and Dissertations

Advanced biomaterials and sophisticated processing technologies aim to fabricate tissue-engineering scaffolds that can predictably interact within a biological environment at a cellular level. Sterilization of such scaffolds is at the core of patient safety and is an important regulatory issue that needs to be addressed prior to clinical translation. In addition, it is crucial that meticulously engineered micro- and nano- structures are preserved after sterilization. Conventional sterilization methods involving heat, steam and radiation are not compatible with engineered polymeric systems because of scaffold degradation and loss of architecture. Using electrospun scaffolds made from polycaprolactone (PCL), a low melting polymer, and ...


Peracetic Acid Sterilization Of Electrospun Polycaprolactone Scaffolds, Suyog Yoganarasimha Jan 2015

Peracetic Acid Sterilization Of Electrospun Polycaprolactone Scaffolds, Suyog Yoganarasimha

Theses and Dissertations

Sterilization of tissue engineered scaffolds is an important regulatory issue and is at the heart of patient safety. With the introduction of new biomaterials and micro/nano structured scaffolds, it is critical that the mode of sterilization preserve these built-in features. Conventional sterilization methods are not optimal for engineered polymeric systems and hence alternate systems need to be identified and validated. PCL is polyester with a low melting point (heat labile), susceptible to hydrolysis and is popular in tissue engineering. Electrospinning generates some nanoscale features within the scaffold, the integrity of which can be affected by sterilization method. Chapter 1 ...


Fem Of Electrospinning Compared To Inkjet Printing Model, Maikel Ghaly Dec 2014

Fem Of Electrospinning Compared To Inkjet Printing Model, Maikel Ghaly

Theses

Electrospinning is a process that uses electrostatic forces to produce nanofibers, or fibers in the nano scale. Nanofibers are widely used in many fields like drug delivery and tissue engineering. Nowadays, it is gaining much attention in the research community as an advantageous process. However, there are many parameters that controlnanofiber formation. This research intends to develop a model of electrospinning on the basis of an inkjet printer technique by using a computer aided simulation (COMSOL). Inkjet printing is a technique that delivers small volumes at high repetitions which can betransported by electrostatic forces through the air onto their intended ...


Design And Fabrication Of A Novel Electrospinning System For Musculoskeletal Tissue Regeneration, Carter L. Chain, Maggie R. Del Ponte, Meng Deng, Feng Yue, Shihuan Kuang Aug 2014

Design And Fabrication Of A Novel Electrospinning System For Musculoskeletal Tissue Regeneration, Carter L. Chain, Maggie R. Del Ponte, Meng Deng, Feng Yue, Shihuan Kuang

The Summer Undergraduate Research Fellowship (SURF) Symposium

Disease and injury to human tissue, especially musculoskeletal tissue, is a prevalent concern to the public, affecting millions of people each year. Current treatment options involving autografts and allografts are hindered by limited availability and risk of immunogenicity, respectively. In order to overcome these limitations, a transdisiplinary regenerative engineering strategy has emerged with a focus on the development of biomimetic scaffolds that closely mimic the properties of the native tissues. For example, the structure of muscle tissue is characterized by oriented muscle fibers. However, fabrication of aligned nanofiber structures that mimic the anisotropic organization of muscle presents significant engineering challenges ...


Characterizing The Reproducibility Of The Properties Of Electrospun Poly(D,L-Lactide-Co-Glycolide) Scaffolds For Tissue-Engineered Blood Vessel Mimics, Toni M. Pipes Jun 2014

Characterizing The Reproducibility Of The Properties Of Electrospun Poly(D,L-Lactide-Co-Glycolide) Scaffolds For Tissue-Engineered Blood Vessel Mimics, Toni M. Pipes

Master's Theses and Project Reports

“Blood vessel mimics” (BVMs) are tissue-engineered constructs that serve as in vitro preclinical testing models for intravascular devices. The Cal Poly Tissue Engineering lab specifically uses BVMs to test the cellular response to stent implantation. PLGA scaffolds are electrospun in-house using the current “Standard Protocol” and used as the framework for these constructs. The performance of BVMs greatly depends on material and mechanical properties of the scaffolds. It is desirable to create BVMs with reproducible properties so that they can be consistent models that ultimately generate more reliable results for intravascular device testing. Reproducibility stems from the consistency of the ...


Humidity Effect On The Structure Of Electrospun Core-Shell Pcl-Peg Fibers For Tissue Regeneration Applications, Adam P. Golin Apr 2014

Humidity Effect On The Structure Of Electrospun Core-Shell Pcl-Peg Fibers For Tissue Regeneration Applications, Adam P. Golin

Electronic Thesis and Dissertation Repository

With the aim of creating a biodegradable scaffold for tympanic membrane (TM) tissue regeneration, core-shell nanofibers composed of a poly(caprolactone) shell and a poly(ethylene glycol) core were created using a coaxial electrospinning technique. In order to create fibers with an optimal core-shell morphology, the effect of relative humidity (RH) on the core-shell nanofibers was systematically studied, with a FITC-BSA complex encapsulated in the core to act as a model protein. The core-shell nanofibers were electrospun at relative humidity values of 20, 25, 30, and 40% RH within a glove box outfitted for humidity control. The core-shell morphology of ...


Mammary Epithelial Cells Cultured Onto Non-Woven Nanofiber Electrospun Silk-Based Biomaterials To Engineer Breast Tissue Models, Yas Maghdouri-White Apr 2014

Mammary Epithelial Cells Cultured Onto Non-Woven Nanofiber Electrospun Silk-Based Biomaterials To Engineer Breast Tissue Models, Yas Maghdouri-White

Theses and Dissertations

Breast cancer is one of the most common types of cancer affecting women in the world today. To better understand breast cancer initiation and progression modeling biological tissue under physiological conditions is essential. Indeed, breast cancer involves complex interactions between mammary epithelial cells and the stroma, both extracellular matrix (ECM) and cells including adipocytes (fat tissue) and fibroblasts (connective tissue). Therefore, the engineering of in vitro three-dimensional (3D) systems of breast tissues allows a deeper understanding of the complex cell-cell and cell-ECM interactions involved during breast tissue development and cancer initiation and progression. Furthermore, such 3D systems may provide a ...


Tissue Engineering Scaffold Fabrication And Processing Techniques To Improve Cellular Infiltration, Casey Grey Jan 2014

Tissue Engineering Scaffold Fabrication And Processing Techniques To Improve Cellular Infiltration, Casey Grey

Theses and Dissertations

Electrospinning is a technique used to generate scaffolds composed of nano- to micron-sized fibers for use in tissue engineering. This technology possesses several key weaknesses that prevent it from adoption into the clinical treatment regime. One major weakness is the lack of porosity exhibited in most electrospun scaffolds, preventing cellular infiltration and thus hosts tissue integration. Another weakness seen in the field is the inability to physically cut electrospun scaffolds in the frontal plane for subsequent microscopic analysis (current electrospun scaffold analysis is limited to sectioning in the cross-sectional plane). Given this it becomes extremely difficult to associate spatial scaffold ...


Developing A Labview Based Thermally Stimulated Current (Tsc) Controller To Measure Residual Charge In Electrospinning, Jai Abhishekh Veezhinathan Dec 2013

Developing A Labview Based Thermally Stimulated Current (Tsc) Controller To Measure Residual Charge In Electrospinning, Jai Abhishekh Veezhinathan

Theses

Electrospinning is an electrohydrodynamic process for the fabrication of nanofibers which are widely used in therapeutical tissue engineering approaches. It utilizes the potential difference of an electrostatic field to overcome the surface tension of the polymer solution to extrude a fine jet of fluid which deposits on the grounded collector as a nanofiber mat. Using this process, nanofibers with diameters less than a micron can be produced.

Previous studies have shown the presence of residual charge in electrospun nanofibers. The presence and decay of residual charge during cell culture media is still unknown. In an attempt to clarify the presence ...


Biomimetic Poly(Ester Amide) Biomaterials For Vascular Tissue Engineering, Darryl K. Knight Dec 2013

Biomimetic Poly(Ester Amide) Biomaterials For Vascular Tissue Engineering, Darryl K. Knight

Electronic Thesis and Dissertation Repository

The focus of this research was to develop a biomimetic, degradable vascular scaffold that could be considered as part of a tissue-engineered vascular graft strategy. A family of degradable poly(ester amide)s (PEAs) derived from naturally occurring α-amino acids, aliphatic diols and diacids were synthesized to yield PEAs with glass transition temperatures below physiologic temperature ensuring their pliability. Tri-functional amino acids l-lysine or l-aspartic acid were incorporated into the polymer backbone yielding complementary functional handles for subsequent conjugation of growth factors. Higher molecular weight PEAs were obtained using an interfacial polycondensation technique compared with a solution polymerization approach.

Human ...


Shelf Life Study Of Electrospun Plga Copolymers, Sean Youra, Nick Hudson Jun 2013

Shelf Life Study Of Electrospun Plga Copolymers, Sean Youra, Nick Hudson

Biomedical Engineering

Poly(lactic-co-glycolic acid) (PLGA) is one of the most commonly used copolymers for electrospinning in tissue engineering applications. However, most research has not focused on the copolymer itself in regards to how long it can be used effectively and if varying the concentrations of polylactic acid (PLA) and polyglycolic acid (PGA) affect the resulting properties. Electrospinning is the method we use to create the three-dimensional constructs, or “scaffolds”, for the blood vessel mimic (BVM) in the tissue engineering lab. The aim of our project was to investigate if the morphology and mechanical properties of the scaffolds changed over time when ...


Sol-Gel Derived Biodegradable And Bioactive Organic-Inorganic Hybrid Biomaterials For Bone Tissue Engineering, Bedilu A. Allo Apr 2013

Sol-Gel Derived Biodegradable And Bioactive Organic-Inorganic Hybrid Biomaterials For Bone Tissue Engineering, Bedilu A. Allo

Electronic Thesis and Dissertation Repository

Treatments of bone injuries and defects have been largely centered on replacing the lost bone with tissues of allogeneic or xenogeneic sources as well as synthetic bone substitutes, which in all lead to limited degree of structural and functional recovery. As a result, tissue engineering has emerged as a viable technology to regenerate the structures and therefore recover the functions of bone tissue rather than replacement alone. Hence, the current strategies of bone tissue engineering and regeneration rely on bioactive scaffolds to mimic the natural extracellular matrix (ECM) as templates onto which cells attach, multiply, migrate and function.

In this ...


Fabricating And Characterizing Physical Properties Of Electrospun Polypeptide-Based Nanofibers, Dhan Bahadur Khadka Jan 2013

Fabricating And Characterizing Physical Properties Of Electrospun Polypeptide-Based Nanofibers, Dhan Bahadur Khadka

Graduate Theses and Dissertations

This dissertation has aimed to fabricate polypeptide based biomaterial and characterize physical properties. Electrospinning is used as a tool for the sample fabrication. Project focused on determining the feasibility of electrospinning of certain synthetic polypeptides and certain elastin-like peptides from aqueous feedstocks and to characterize physical properties of polymer aqueous solution, cast film and spun fibers and fiber mats. The research involves peptide design, polymer electrospinning, fibers crosslinking, determining the extent of crosslinking, fibers protease degradation study, fibers stability and self-organization analysis, structure and composition determination by various spectroscopy and microscopy techniques and characterization of mechanical properties of individual suspended ...


The Effects Of Matrix Elasticity, Composition, And Exogenous Growth Factors On The Vascular Differentiation Of Mesenchymal Stem Cells, Kathryn Anne Wingate Jan 2013

The Effects Of Matrix Elasticity, Composition, And Exogenous Growth Factors On The Vascular Differentiation Of Mesenchymal Stem Cells, Kathryn Anne Wingate

Mechanical Engineering Graduate Theses & Dissertations

Cardiovascular diseases are the leading cause of death in the western world. Regeneration of functional vascular tissue remains a critical barrier to successful treatment of these diseases. Attempts to produce functional vascular tissue with autogenous vascular cells have limited success due to the need for invasive surgery. Mesenchymal stem cells (MSCs) are a powerful cellular alternative for vascular regeneration as they are easily obtainable, multipotent, and thrombo-resistant. Currently, the mechanisms that drive MSC differentiation to healthy or diseased vascular phenotypes are not well understood. There is a critical need to define the factors in the cellular microenvironment that guide MSC ...


Nanomechanics Of Electrospun Nanofibres For Tissue Engineering Of The Tympanic Membrane, Sara Makaremi Dec 2012

Nanomechanics Of Electrospun Nanofibres For Tissue Engineering Of The Tympanic Membrane, Sara Makaremi

Electronic Thesis and Dissertation Repository

The Tympanic Membrane (TM), also known as the eardrum, includes layers of organized collagen nanofibres which play an essential role in sound transmission. Perforations that are caused by infection or accident must be repaired in order to restore hearing. Tympanoplasty is performed using grafts that are prepared from bladder, cartilage, temporal fascia and cadaveric skin. However, since mechanical properties of these grafts do not match those of the original TM, normal hearing is not fully restored. The goal of this study is to develop nanofibrous scaffolds for tissue engineering of the TM in order to circumvent the complications addressed with ...