Biomedical Engineering and Bioengineering Commons^™

Ccr4-Bearing T Cells Participate In Autoimmune Diabetes., Soon H. Kim, Mary M. Cleary, Howard S. Fox, Icos Coporation, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Chemokine receptor expression is exquisitely regulated on T cell subsets during the course of their migration to inflammatory sites. In the present study we demonstrate that CCR4 expression marks a pathogenic population of autoimmune T cells. CCR4 was found exclusively on memory CD4(+) T cells during the progression of disease in NOD mice. Cells expressing the CCR4 ligand TARC (thymus- and activation-regulated chemokine) were detected within infiltrated islets from prediabetic mice. Interestingly, neutralization of macrophage-derived chemokine (MDC) with Ab caused a significant reduction of CCR4-positive T cells within the pancreatic infiltrates and inhibited the development of insulitis and diabetes. Furthermore, …

Weibull Model Development For Fatigue Crack Growth, Bobby Day, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

This effort is an attempt to "Weibullize" fatigue crack growth rate data as a function of stress intensity range (ΔΚ). It is not a classical Weibull application in that it does not address a population but rather treats rate of crack growth as a fraction of the highest rate of crack growth rate in the data set (usually the final data point of the set) as the "failed fraction of a population." All da/dN values are converted to a percentage or fraction of the 100% data value, processed into a linear relationship and plotted against the natural log of the …

A Review Of Nanotechnology Applications, Nick Ledbetter, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

A review of current technology research efforts has been conducted in this paper. In nanoscience, objects are measured in nanometers, 1 billionth of a meter. For comparison, a human hair is approximately 100,000 nanometers thick and the smallest feature on current computer chips measures about 200 nanometers. Since nanotechnology has a wide range of applications from computer chip manufacturing, new materials, renewable energy, interconnection technology to mount the nano-chips, and in medical science, this review presents a broad summary of development efforts in the government and private sector laboratories. Therefore this project on the one hand prepares a review of …

Implementation Of Gy-Eq For Deterministic Effects Limitation In Shield Design, John W. Wilson, Myung-Hee Y. Kim, Giovanni De Angelis, Francis A. Cucinotta, Nobuaki Yoshizawa, Francis F. Badavi

Mathematics & Statistics Faculty Publications

The NCRP has recently defined RBE values and a new quantity (Gy-Eq) for use in estimation of deterministic effects in space shielding and operations. The NCRP's RBE for neutrons is left ambiguous and not fully defined. In the present report we will suggest a complete definition of neutron RBE consistent with the NCRP recommendations and evaluate attenuation properties of deterministic effects (Gy-Eq) in comparison with other dosimetric quantities.

Mechanism Of Preconditioning By Isoflurane In Rabbits: A Direct Role For Reactive Oxygen Species, Katsuya Tanaka, Dorothee Weihrauch, Franz Kehl, Lynda M. Ludwig, John F. Ladisa, Judy R. Kersten, Paul S. Pagel, David C. Warltier

Biomedical Engineering Faculty Research and Publications

LARGE quantities of reactive oxygen species (ROS) released during reperfusion after coronary artery occlusion damage proteins responsible for intracellular homeostasis, produce tissue injury, depress contractile function, and increase myocardial infarct size. In contrast, small quantities of ROS may exert beneficial effects during ischemia and reperfusion when released before a prolonged ischemic event. ROS derived from mitochondria during a brief ischemic episode produce preconditioning. Free radical scavengers administered during ischemic preconditioning (IPC) markedly attenuate the protective effect of the preconditioning stimulus on infarct size. These data suggest that IPC is mediated in part by small quantities of ROS released during preconditioning. …

Lunar Lava Tube Radiation Safety Analysis, Giovanni De Angelis, J. W. Wilson, M. S. Clowdsley, J. E. Nealy, D. H. Humes, J. M. Clem

Mathematics & Statistics Faculty Publications

For many years it has been suggested that lava tubes on the Moon could provide an ideal location for a manned lunar base, by providing shelter from various natural hazards, such as cosmic radiation, meteorites, micrometeoroids, and impact crater ejecta, and also providing a natural environmental control, with a nearly constant temperature, unlike that of the lunar surface showing extreme variation in its diurnal cycle. An analysis of radiation safety issues on lunar lava tubes has been performed by considering radiation from galactic cosmic rays (GCR) and Solar Particle Events (SPE) interacting with the lunar surface, modeled as a regolith …

Characterization Of Engineered Human Lung Tissue, Benjamin Boytor

FIU Electronic Theses and Dissertations

Characterizing engineered human lung tissue is an important step in developing a functional tissue replacement for lung tissue repair and in vitro analysis. Small tissue constructs were grown by seeding IMR-90 fetal lung fibroblasts and adult microvascular endothelial cells onto a Polyglycolic acid (PGA) polymer template. Introducing the constructs to dynamic culture conditions inside a bioreactor facilitated three-dimensional growth seen in scanning electron microscopy images (SEM).

Characterization of the resultant tissue samples was done using SEM imagery, tensile tests, and biochemical assays to quantify extra-cellular matrix (ECM) composition. Tensile tests of the engineered samples indicated an increase in the mechanical …

Mineralization Of Manure Nutrients, Bahman Eghball, Brian J. Wienhold, John E. Gilley, Roger A. Eigenberg

Biological Systems Engineering: Papers and Publications

In order to apply manure or compost to fulfill the nutrient requirements of a crop, knowledge of the amount of nutrients mineralized following application is needed. Nutrient mineralization from applied manure depends on temperature, soil moisture, soil properties, manure characteristics, and microbial activity. Since these factors cannot be accurately predicted, nutrient mineralization from applied manure can only be approximated. Nitrogen (N) availability from applied manure includes the inorganic N (NO₃-N and NH₄-N) in manure plus the amount of organic N mineralized following application. Nitrogen mineralization differs for different manure types since the inorganic/organic fraction and quality …

A Rehabilitation Engineering Course For Biomedical Engineers, M. Barbara Silver-Thorn

Biomedical Engineering Faculty Research and Publications

This paper describes an upper division elective course in rehabilitation engineering that addresses prosthetics and orthotics, wheelchair design, seating and positioning, and automobile modifications for individuals with disabilities. Faculty lectures are enhanced by guest lectures and class field trips. Guest lecturers include a prosthetist and a lower extremity amputee client, an engineer/prosthetist specializing in the upper extremity, and a rehabilitation engineer. The lower extremity prosthetist and his client present a case study for prosthetic prescription, fabrication, fitting, alignment, and evaluation. The engineer/prosthetist contrasts body-powered versus externally powered upper extremity prostheses and associated design, fitting, and functional considerations; he also discusses …

The Impact Of Variations In Membrane Capacitance On The Detected Neural-Electronic Signal, Robert B. Szlavik

Biomedical Engineering

There have been various theoretical and experimental studies presented in the literature that focus on interfacing neurons with discrete electronic devices such as transistors. The demonstrated lack of reproducible fidelity of the nerve cell action potential at the device junction would make it impractical to implement these devices in any neural prosthetic application where reliable detection of the action potential was a pre-requisite. In this study, the impact of typical variations in membrane capacitance on the detected neural signal is investigated.

Bme Stats Fall 2002, Department Of Biomedical Engineering, Florida International University

STATS

No abstract provided.

Evaluation Of 70/30 D,L-Pla For Use As A Resorbable Interbody Fusion Cage, Jeffrey M. Toth, Mei Wang, Jeffrey L. Scifert, G. Bryan Cornwall, Bradley T. Estes, Howard Seim, Simon Turner

Biomedical Engineering Faculty Research and Publications

Titanium lumbar interbody spinal fusion devices are reported to be 90% effective for single-level lumbar interbody fusion, although radiographic determination of fusion has been debated. Using blinded radiographic, biomechanic, histologic, and statistical measures, researchers in the present study evaluated a radiolucent 70/30 poly(L-lactide-co-D,L-lactide) (70/30 D,L-PLa) interbody fusion device packed with autograft or rhBMP-2 on a collagen sponge in 25 sheep at 3, 6, 12, 18, and 24 months. A trend of increased fusion stiffness, radiographic fusion, and histologic fusion was demonstrated from 3 months to 24 months. Device degradation was associated with a mild to moderate chronic inflammatory response at …

Methods Of Modifying Lignin In Plants By Transformation With A 4-Coumarate Coenzyme A Ligase Nucleic Acid, Vincent Lee C. Chiang, Chung-Jui Tsai, Wen-Jing Hu

Michigan Tech Patents

The invention pertains to methods of altering growth, lignin content, coniferyl and sinapyl alcohol units in the lignin structure, disease resistance and cellulose content in plants by transformation with a lignin pathway p-coumarate Co-enzyme A ligase (4CL) nucleic acid.

Ethyl Tert-Butyl Ether And Methyl Tert-Butyl Ether: Status, Review, And Alternative Use Exploring The Environmental Issues Of Mobile, Recalcitrant, Hossein Noureddini

Papers in Biomaterials

Petroleum products leaking from under ground storage tanks have raised concerns regarding the quality of ground water resources, The concerns about the environmental behavior and rate of MTBE as an oxygenated additive prompted this iuvestigation to explore the technical characteristics of MTBE in comparison to ETBF. Evaluation of the existing literature suggests that ETBE has more favorable characteristics than MTBE. Findings in this research suggest that ETBE is a technically sound oxygenated octane enhancer, which can help refiners meet specificatios for cleaner burning gasoline.

An Automated Coronary Artery Occlusion Device For Stimulating Collateral Development In Vivo, Richard Rys, John F. Ladisa, John P. Tessmer, Weidong Gu, Judy R. Kersten, David C. Warltier, Paul S. Pagel

Biomedical Engineering Faculty Research and Publications

Introduction: Repetitive, brief coronary artery occlusions produce collateral development in experimental animals. This model causes coronary collateralization in a highly reproducible fashion, but the process is very labor intensive. We report the design and use of a fully automated hydraulic coronary occlusion device capable of producing repetitive coronary occlusions and enhancement of coronary collateral development in dogs.

Methods: The device consists of analog electronics that allow adjustment of occlusion number, frequency, pressure and duration, and mechanical components responsible for the coronary occlusion. The motor and piston of the device are coupled to a chronically implanted hydraulic vascular occluder …

Investigation Of Lower-Limb Tissue Perfusion During Loading, Barbara Silver-Thorn

Biomedical Engineering Faculty Research and Publications

An extant tissue indentor used for amputee residual limb tissue indentation studies was modified to include laser Doppler flowmetry (LDF) to enable measurement of tissue perfusion during indentation. This device allows quantitative assessment of the mechanical and physiological response of soft tissues to load, as demonstrated by indentation studies of the lower-limb tissues of young healthy subjects. Potential measures of interest include the relative change in tissue perfusion with load and the time delays associated with the perfusion response during tissue loading and unloading. Such measures may prove useful in future studies of residual limb tissues, improving our understanding of …

Accurate Quantification Of Steady And Pulsatile Flow With Segmented K-Space Magnetic Resonance Velocimetry, Haosen Zhang, Sandra S. Halliburton, James R. Moore, Orlando P. Simonetti, Paulo R. Schvartzman, Richard D. White, George P. Chatzimavroudis

Chemical & Biomedical Engineering Faculty Publications

Conventional non-segmented magnetic resonance phase velocity mapping (MRPVM) is an accurate but relatively slow velocimetric technique. Therefore, the aim of this study was to evaluate the accuracy of the much faster segmented k-space MRPVM in quantifying flow. The axial velocity was measured in four straight tubes (inner diameter: 5.6–26.2 mm), using a segmented MRPVM sequence with seven lines of k-space per segment. The flow rate and flow volume were accurately quantified (errorssteady (r²=0.99) and pulsatile flow (r²=0.98), respectively. The measured velocity profiles and flow rates from the segmented sequence agreed …

Comment On "Quadriceps Protects The Anterior Cruciate Ligament", Antonie J. Van Den Bogert

Mechanical Engineering Faculty Publications

No abstract provided.

Residual Effects Of Compost And Fertilizer Applications On Nutrients In Runoff, John E. Gilley, Bahman Eghball

Biological Systems Engineering: Papers and Publications

The application of compost or fertilizer at rates that exceed crop nutrient requirements can result in phosphorus (P) and nitrogen (N) accumulation in soil. This study was conducted to determine the influence of soil P and N contents on the concentrations and total amounts of P and N transported in runoff. Composted beef cattle feedlot manure or inorganic fertilizer were added from 1992 to 1995 to a Sharpsburg silty clay loam soil at rates sufficient to meet P or N requirements for corn and incorporated following application. After four years of corn production following the last compost application, P concentration, …

Masseter Muscle Activity Resulting From Stimulation Of Hypothalamic Behavioral Sites : Wavelet Analysis, Anne Marie Petrock

Theses

Patterns of electromyographic (EMG) activity can give an insight into muscle activity associated with a given behavioral state. The masseter muscle is positioned closely to the temporomandibular joint and controls the position and movement of the jaw. The hypothalamus is the region of the brain associated with emotional behavior. In an effort to further understand the muscle activity underlying emotional display, the hypothalamus in two cats was stimulated to evoke a stereotyped emotional response, known as the "rage response." Unsheathing of the claws, retraction of the ears, significant pupillary dilation and vocalization (hissing) characterize this behavior. EMG data obtained at …

Quantitative Assessment Of Reflex Blood Pressure Regulation Using A Dynamic Model Of The Cardiovascular System, Tanha Patel

Theses

A quantitative understanding of the changes in coronary, pulmonary and systemic hemodynamic variables and their effects on the regulation mechanism is important to the better postoperative management of patients with impaired cardiac function. The arterial baroreflex plays a key role in blood pressure homeostasis, and its impairment may result in exaggerated blood pressure fluctuations and an increased risk of cardiovascular morbid events.

The objective of this work was to construct a mathematical model of the cardiovascular system, which will allow us to simulate the effects of the baroreceptor reflex regulation on sudden changes in blood pressure, caused by sudden changes …

Cortical Response To Facial Expressions Of Young Adult Males With Autism Spectrum Disorders And Controls Using Functional Magnetic Resonance Imaging (Fmri), Jagriti Arora

Theses

Autism is a neurodevelopmental disorder that is defined by deficits in social and emotional impairments and this study aims to identify specific brain regions involved during facial processing. The simple task of focusing on the face during social interactions for the normal group is found difficult by the autistic group.

In this study, functional magnetic resonance imaging (fMRI) was used as subjects performed two experimental tasks (EXPLICIT and IMPLICIT) in which a series of photographs of nine males and nine females displaying three affective states (6 fear, 6 happy and 6 neutral) and six scrambled- face control stimuli were presented …

Dimensional Study Of Desaminotyrosyl Polyarylates, Kalsank Vaishali Pai

Theses

Synthetic polymers have found widespread usage and acceptance in various areas of the medical device industry. The recognition that no polymeric biornaterials are truly biocompatible has led to the search for bioerodible materials with desirable biological and physical properties for use as tissue engineering scaffolds and to enable in-vivo devices. Several methodologies have been tried and tested to invent the "ideal" biopolymer. The ideal polymeric biomaterial would be one that offers a favorable response at both the cellular and the systemic level with minimum alteration to its mechanical properties and chemistry. The systematic investigation of process-structure-property space to determine the …

Ua3/9/5 Complex For Engineering & Biological Sciences Groundbreaking, Wku President's Office

WKU Archives Records

Remarks delivered by WKU president Gary Ransdell at the groundbreaking of the Complex for Engineering & Biological Sciences.

Molecular Characterization Of The T(4;9)12gso Mutation And Analysis Of The Associated Fitness, Skeletal, And Lymphoproliferative Phenotypes, Laura Ray Chittenden

Doctoral Dissertations

The t(4;9)(B3;E3.2)12Gso reciprocal translocation is an autosomal recessive mouse mutation involving chromosome 4, band B3 (Mmu4B3) and chromosome 9, band E3.2 (Mmu9E3.2). The most striking phenotype of the 12Gso homozygote involves axial skeleton deformities, resulting in significant shortening of body length, scoliosis, displaced hips and kinky tails. Homozygous animals are significantly smaller than normal littermates and frequently exhibit early lethality, as many of the mutants perish by 1 day postnatal, and many others do not survive the three weeks to weaning age. Homozygous 12Gso mice also develop an unusual bone marrow defect that closely resembles leukemia. The penetrance of the …

Survey Of Hydrogen Sulfide Concentrations In Vicinity Of Beef Cattle Feedlots, Richard K. Koelsch, Bryan L. Woodbury, David E. Stenberg, Daniel N. Miller, Dennis D. Schulte

Department of Biological Systems Engineering: Conference Presentations and White Papers

A field survey of Total Reduced Sulfur (TRS) concentrations in the vicinity of beef cattle feedlots was conducted to compare field observations against current regulatory thresholds. In addition, environmental factors that may contribute to increased TRS emissions were evaluated. It was observed that TRS levels in the vicinity of beef cattle feedlots are not likely to exceed current regulatory thresholds used by mid-western states. It was further noted that concentration of TRS varies with air temperature and time of day. However, wet feedlot surface conditions and wind speed had almost no impact upon observed concentrations.

Functional Profiling Of The Saccharomyces Cerevisiae Genome, Patrick Flaherty

Patrick Flaherty

Fluoride Release From Model Glass Ionomer Cements, A. Guida, R. G. Hill, Mark R. Towler, S. Eramo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Glass ionomer cements (GICs) are an important class of biomedical material used extensively for color matched mercury free, dental restorations. GICs can release clinically beneficial amounts of fluoride and have acceptable handling properties which make them suitable as dental restoratives. The fluoride release of model GICs produced from specially synthesized fluoro-alumino-silicate glasses was studied. Nine glasses of varying fluoride content based on 4.5SiO₂-3Al₂O₃-1.5P₂O₅-(5-Z)CaO-ZCaF₂ were synthesized, and cement disks were prepared from them. The glass transition temperature reduced with increasing fluorine content of the glass. Fluoride ion release was measured …

A Preliminary Study Of An Aluminum-Free Glass Polyalkenoate Cement, Mark R. Towler, C. M. Crowley, D. Murphy, A. M.C. O'Callaghan

Chemical and Biochemical Engineering Faculty Research & Creative Works

No abstract provided.

Spontaneous Planarization Of Nanoscale Phase Separated Thin Film, Ravi F. Saraf, Sanjun Niu, Eric Stumb

Ravi Saraf Publications

Structure of complex fluid at mesoscales is influenced by interfacial effects. We describe the dynamic response in such films to sudden change in interfacial tension. In a self-assembled block copolymer film, the monolayer of 15 nm diam cylindrical discrete phases close to the surface commence to sink at an average rate of 0.16 nm/day in response to the interfacial tension change. Surprisingly, this spontaneous planarization occurs, even though the cylinders are covalently stitched to the matrix. A simple model explains the observed behavior. The observation may lead to approaches to tailor the structure of mesoscale thin films of complex fluids …