Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Louisiana Tech University (23)
- Purdue University (17)
- University at Albany, State University of New York (14)
- University of Arkansas, Fayetteville (13)
- Old Dominion University (12)
-
- University of Nebraska - Lincoln (11)
- Selected Works (8)
- University of New Mexico (8)
- Western University (8)
- Florida International University (7)
- Washington University in St. Louis (7)
- City University of New York (CUNY) (6)
- Harrisburg University of Science and Technology (5)
- Wayne State University (5)
- Michigan Technological University (4)
- Chapman University (3)
- University of Kentucky (3)
- University of Massachusetts Amherst (3)
- University of South Florida (3)
- University of Tennessee, Knoxville (3)
- University of Texas Rio Grande Valley (3)
- Virginia Commonwealth University (3)
- California Polytechnic State University, San Luis Obispo (2)
- Louisiana State University (2)
- Minnesota State University, Mankato (2)
- Southern Methodist University (2)
- University of Connecticut (2)
- Air Force Institute of Technology (1)
- Boise State University (1)
- Brigham Young University (1)
- Keyword
-
- Nanotechnology (12)
- Nanoparticles (10)
- Cancer (9)
- Applied sciences (8)
- Drug delivery (7)
-
- Biomaterials (6)
- DNA (6)
- Health and environmental sciences (6)
- Nanomedicine (6)
- Tissue engineering (6)
- Biological sciences (5)
- Nanofabrication (5)
- Nanomaterials (5)
- Tissue Engineering (5)
- Biosensors (4)
- Drug Delivery (4)
- Electrospinning (4)
- MEMS (4)
- Microfluidics (4)
- Nanobiotechnology (4)
- 3D Printing (3)
- BioMEMS (3)
- Biosensor (3)
- Carbon nanotubes (3)
- Cellulose Nanocrystals (3)
- Collagen (3)
- DNA nanotechnology (3)
- Electroporation (3)
- Fluorescence (3)
- Hybridization (3)
- Publication Year
- Publication
-
- Doctoral Dissertations (28)
- Legacy Theses & Dissertations (2009 - 2024) (14)
- Bioelectrics Publications (7)
- Electronic Thesis and Dissertation Repository (7)
- Graduate Theses and Dissertations (7)
-
- Other Nanotechnology Publications (7)
- FIU Electronic Theses and Dissertations (6)
- Nanoscience and Microsystems ETDs (6)
- Faculty Works (5)
- The Summer Undergraduate Research Fellowship (SURF) Symposium (5)
- Theses and Dissertations (5)
- Open Access Dissertations (4)
- Wayne State University Dissertations (4)
- Biomedical Engineering Theses & Dissertations (3)
- Department of Mechanical and Materials Engineering: Faculty Publications (3)
- Heather Clark (3)
- McKelvey School of Engineering Theses & Dissertations (3)
- Pharmacy Faculty Articles and Research (3)
- Publications and Research (3)
- Research Symposium (3)
- USF Tampa Graduate Theses and Dissertations (3)
- Arts & Sciences Electronic Theses and Dissertations (2)
- Aviation Department Publications (2)
- Dissertations, Master's Theses and Master's Reports (2)
- Dissertations, Master's Theses and Master's Reports - Open (2)
- Dissertations, Theses, and Capstone Projects (2)
- Electrical & Systems Engineering Publications and Presentations (2)
- Electronic Theses and Dissertations (2)
- Honors Scholar Theses (2)
- Matteo Rinaldi (2)
- Publication Type
- File Type
Articles 181 - 195 of 195
Full-Text Articles in Biomedical Engineering and Bioengineering
Gold Nanorod-Mediated Photothermolysis Induces Apoptosis Of Macrophages Via Damage Of Mitochondria, Ling Tong, Ji-Xin Cheng
Gold Nanorod-Mediated Photothermolysis Induces Apoptosis Of Macrophages Via Damage Of Mitochondria, Ling Tong, Ji-Xin Cheng
Other Nanotechnology Publications
Aims: Induction of apoptosis or necrosis in activated macrophages by gold nanorod-mediated photothermolysis is demonstrated and the mechanisms underlying the processes are investigated. Materials & methods: Gold nanorods were functionalized with cysteine-octaarginine peptides (R8-NRs). Uptake of R8-NRs by activated macrophages was monitored by two-photon luminescence imaging. The laser irradiation conditions were controlled to induce apoptosis or necrosis to R8-NR-internalized macrophages. Mitochondrial damage and reactive oxygen species overproduction during photothermolysis was investigated by confocal fluorescence microscopy and transmission-electron microscopy. Results: Activated macrophages efficiently uptake R8-NRs both in vitro and in live animals. Laser irradiation of internalized nanorods with controlled power density …
Gold Nanorods As Contrast Agents For Biological Imaging: Optical Properties, Surface Conjugation And Photothermal Effects, Ling Tong, Qingshan Wei, Alexander Wei, Ji-Xin Cheng
Gold Nanorods As Contrast Agents For Biological Imaging: Optical Properties, Surface Conjugation And Photothermal Effects, Ling Tong, Qingshan Wei, Alexander Wei, Ji-Xin Cheng
Other Nanotechnology Publications
Gold nanorods (NRs) have plasmon-resonant absorption and scattering in the near-infrared (NIR) region, making them attractive probes for in vitro and in vivo imaging. In the cellular environment, NRs can provide scattering contrast for darkfield microscopy, or emit a strong two-photon luminescence due to plasmon-enhanced two-photon absorption. NRs have also been employed in biomedical imaging modalities such as optical coherence tomography or photoacoustic tomography. Careful control over surface chemistry enhances the capacity of NRs as biological imaging agents by enabling cell-specific targeting, and by increasing their dispersion stability and circulation lifetimes. NRs can also efficiently convert optical energy into heat, …
Continuous Glucose Monitoring Microsensor With A Nanoscale Conducting Matrix And Redox Mediator, Daniel Pesantez
Continuous Glucose Monitoring Microsensor With A Nanoscale Conducting Matrix And Redox Mediator, Daniel Pesantez
Legacy Theses & Dissertations (2009 - 2024)
The major limiting factor in kidney clinical transplantation is the shortage of transplantable organs. The current inability to distinguish viability from non-viability on a prospective basis represents a major obstacle in any attempt to expand organ donor criteria. Consequently, a way to measure and monitor a relevant analyte to assess kidney viability is needed. For the first time, the initial development and characterization of a metabolic microsensor to assess kidney viability is presented. The rate of glucose consumption appears to serve as an indicator of kidney metabolism that may distinguish reversible from irreversible kidney damage. The proposed MetaSense (Metabolic Sensor) …
Release Of Hydrophobic Molecules From Polymer Micelles Into Cell Membranes Revealed By Forster Resonance Energy Transfer Imaging, Hongtao Chen, Sungwon Kim, Shuyi Wang, Kinam Park, Ji-Xin Cheng
Release Of Hydrophobic Molecules From Polymer Micelles Into Cell Membranes Revealed By Forster Resonance Energy Transfer Imaging, Hongtao Chen, Sungwon Kim, Shuyi Wang, Kinam Park, Ji-Xin Cheng
Other Nanotechnology Publications
it is generally assumed that polymeric micelles, upon administration into the blood stream, carry drug molecules until they are taken up into cells followed by intracellular release. The current work revisits this conventional wisdom. The study using dual-labeled micelles containing fluorescently labeled copolymers and hydrophobic fluorescent probes entrapped in the polymeric micelle core showed that cellular uptake of hydrophobic probes was much faster than that of labeled copolymers. This result implies that the hydrophobic probes in the core are released from micelles in the extracellular space. Forster resonance energy transfer (FRET) imaging and spectroscopy were used to monitor this process …
Gold Nanorods Mediate Tumor Cell Death By Compromising Membrane Integrity, Ling Tong, Yan Zhao, Terry B. Huff, Matthew N. Hansen, Alexander Wei, Ji-Xin Cheng
Gold Nanorods Mediate Tumor Cell Death By Compromising Membrane Integrity, Ling Tong, Yan Zhao, Terry B. Huff, Matthew N. Hansen, Alexander Wei, Ji-Xin Cheng
Other Nanotechnology Publications
Folate-conjugated gold nanorods targeted to tumor cell surfaces produced severe membrane damage upon near-infrared irradiation. Photoinduced injury to the plasma membrane resulted in a rapid increase in intracellular calcium (shown in green) with subsequent disruption of the actin network, featured prominently by the formation of membrane blebs.
In Vivo Quantitation Of Rare Circulating Tumor Cells By Multiphoton Intravital Flow Cytometry, Wei He, Haifeng Wang, Lynn C. Hartmann, Ji-Xin Cheng, Phillip S. Low
In Vivo Quantitation Of Rare Circulating Tumor Cells By Multiphoton Intravital Flow Cytometry, Wei He, Haifeng Wang, Lynn C. Hartmann, Ji-Xin Cheng, Phillip S. Low
Other Nanotechnology Publications
Quantitation of circulating tumor cells (CTCs) constitutes an emerging tool for the diagnosis and staging of cancer, assessment of response to therapy, and evaluation of residual disease after surgery. Unfortunately, no existing technology has the sensitivity to measure the low numbers of tumor cells (< 1 CTC per ml of whole blood) that characterize minimal levels of disease. We present a method, intravital flow cytometry, that noninvasively counts rare CTCs in vivo as they flow through the peripheral vasculature. The method involves i.v. injection of a tumor-specific fluorescent ligand followed by multiphoton fluorescence imaging of superficial blood vessels to quantitate the flowing CTCs. Studies in mice with metastatic tumors demonstrate that CTCs can be quantitated weeks before metastatic disease is detected by other means. Analysis of whole blood samples from cancer patients further establishes that human CTCs can be selectively labeled and quantitated when present at approximate to 2 CTCs per ml, opening opportunities for earlier assessment of metastatic disease.
Hyperthermic Effects Of Gold Nanorods On Tumor Cells, Terry B. Huff, Ling Tong, Matthew N. Hansen, Ji-Xin Cheng, Alexander Wei
Hyperthermic Effects Of Gold Nanorods On Tumor Cells, Terry B. Huff, Ling Tong, Matthew N. Hansen, Ji-Xin Cheng, Alexander Wei
Other Nanotechnology Publications
Plasmon-resonant gold nanorods, which have large absorption cross sections at near-infrared frequencies, are excellent candidates as multifunctional agents for image-guided therapies based on localized hyperthermia. The controlled modification of the surface chemistry of the nanorods is of critical importance, as issues of cell-specific targeting and nonspecific uptake must be addressed prior to clinical evaluation. Nanorods coated with cetyltrimethylammonium bromide (a cationic surfactant used in nanorod synthesis) are internalized within hours into KB cells by a nonspecific uptake pathway, whereas the careful removal of cetyltrimethylammonium bromide from nanorods functionalized with folate results in their accumulation on the cell surface over the …
Desulfovibrio Desulfuricans G20 Tetraheme Cytochrome Structure At 1.5 A˚ And Cytochrome Interaction With Metal Complexes, Mrunalini Pattarkine, J J. Tanner, C A. Bottoms, Y H. Lee, Judy D. Wall
Desulfovibrio Desulfuricans G20 Tetraheme Cytochrome Structure At 1.5 A˚ And Cytochrome Interaction With Metal Complexes, Mrunalini Pattarkine, J J. Tanner, C A. Bottoms, Y H. Lee, Judy D. Wall
Faculty Works
The structure of the type I tetraheme cytochrome c3 from Desulfovibrio desulfuricans G20 was determined to 1.5 A˚ by X-ray crystallography. In addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. Only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate. Alterations in the overall conformation and thermostability of the metal-oxidized protein were investigated …
Prospective Energy Densities In The Forisome, A New Smart Material, William Pickard, Michael Knoblauch, Winfried Peters, Amy Shen
Prospective Energy Densities In The Forisome, A New Smart Material, William Pickard, Michael Knoblauch, Winfried Peters, Amy Shen
Winfried S. Peters
In Vitro And In Vivo Two-Photon Luminescence Imaging Of Single Gold Nanorods, Haifeng Weng, Terry B. Huff, Daniel A. Zweifel, Wei He, Philip S. Low, Alexander Wei, Ji-Xin Cheng
In Vitro And In Vivo Two-Photon Luminescence Imaging Of Single Gold Nanorods, Haifeng Weng, Terry B. Huff, Daniel A. Zweifel, Wei He, Philip S. Low, Alexander Wei, Ji-Xin Cheng
Other Nanotechnology Publications
Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.
Atp-Independent Contractile Proteins From Plants, Michael Knoblauch, Gundula Noll, Torsten Müller, Dirk Prüfer, Ingrid Schneider-Hüther, Dörte Scharner, Aart Van Bel, Winfried Peters
Atp-Independent Contractile Proteins From Plants, Michael Knoblauch, Gundula Noll, Torsten Müller, Dirk Prüfer, Ingrid Schneider-Hüther, Dörte Scharner, Aart Van Bel, Winfried Peters
Winfried S. Peters
Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh
Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh
Faculty Works
The formation of composite films of double-stranded DNA and cationic lipid molecules (octadecylamine, ODA) and the hybridization of complementary single-stranded DNA molecules in such composite films are demonstrated. The immobilization of DNA is accomplished by simple immersion of a thermally evaporated ODA film in the DNA solution at close to physiological pH. The entrapment of the DNA molecules in the cationic lipid film is dominated by attractive electrostatic interaction between the negatively charged phosphate backbone of the DNA molecules and the protonated amine molecules in the thermally evaporated film and has been quantified using quartz crystal microgravimetry (QCM). Fluorescence studies …
Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh
Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh
Faculty Works
Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.
Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh
Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh
Faculty Works
The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …
Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh
Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh
Faculty Works
Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …