Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021,
2022
Faculty of Veterinary Science
Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott
Chulalongkorn University Theses and Dissertations (Chula ETD)
PEDV and PDCoV are recognized as important pathogens causing enteric disease in swine herds worldwide. During 2014-2015, several outbreaks of PEDV occurred in many provinces in Thailand, and the clinical signs were more severe than those of classical PEDV in Thailand during 2008-2012. Therefore, the new introduction of PEDV or mutated virus strains was suspected. In 2015, PDCoV was also reported in Thailand, making the situation of enteric disease in the Thai swine industry worse. Since genetic classification studies of PEDV and PDCoV had never been done in Thailand before, this study aimed to investigate the genetic diversity of PEDV …
Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test,
2022
Faculty of Veterinary Science
Estimating Canine Distemper Virus Exposure In Captive Carnivores In Thai Zoos Using A Pseudotype-Based Serum Neutralization Test, Erngsiri Kaewkhunjob
Chulalongkorn University Theses and Dissertations (Chula ETD)
Despite its name, Canine distemper virus (CDV) infection is not restricted to canids but extends to several species of the order Carnivora. The extent of CDV exposure in captive carnivores in Thailand has never been estimated before. Therefore, the objective of this study is to estimate CDV exposure in captive carnivores in Thai zoos using a pseudotype-based neutralization assay (SNT). A total of 264 archived serum samples collected between 2005 and 2020 from captive carnivore species from 6 zoos of the Zoological Park Organization of Thailand (ZPOT) (211 samples) and Tiger Kingdom (TK) (53 samples) were tested in this study. …
Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal,
2022
Faculty of Veterinary Science
Situation Analysis And Kap On Antimicrobial Use And Resistance Among Veterinarians And Broiler Poultry Farmers Of Nepal, Manoj Kumar Shahi
Chulalongkorn University Theses and Dissertations (Chula ETD)
The primary goal of this research was to assess the situation analysis and knowledge, attitude, and practices (KAP) of poultry practitioner veterinarians (PPV) and broiler poultry farmers (BPF) regarding antimicrobial use (AMU) and antimicrobial resistance (AMR) in Nepal. The study area covered approximately 88.1% of Nepal's poultry population. A total of 327 PPV from 56 districts and 500 BPF from 40 districts of seven provinces participated. AMU situation and demographic information were collected and analyzed using descriptive statistics and logistic regression analysis to determine associations between variables. Most PPV were male (85.0%) with a mean age of 31.9±7.8 years. Half …
Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys,
2022
Faculty of Veterinary Science
Associations Of Feline Paramyxoviruses With Pathological Lesions In Cat Kidneys, Aisyah Nikmatuz Zahro
Chulalongkorn University Theses and Dissertations (Chula ETD)
Paramyxoviruses in cats composing of feline paramyxovirus (FPaV), feline morbillivirus genotype 1 (FeMV-1), and feline morbillivirus genotype 2 (FeMV-2). These viruses had been recently suggested to be associated with kidney disease in cats. However, pathological consequence of infection is remained restricted. This study aimed to investigate the association of paramyxovirus infection and pathological consequence in cats’ kidney. Renal fresh tissues were collected from routine necropsy in Department of Pathology, Faculty of Veterinary Science, Then, tissues were histopathologically categorized into normal group and pathologic group. To screen paramyxovirus infection, reverse-transcription polymerase chain reaction (RT-PCR) assays using Res-Mor-Hen pan-primer set and specific …
The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species,
2022
Faculty of Veterinary Science
The Infection Dynamics, Co-Infection, Multi-Gene Phylogeny, And Molecular Screening Of Plasmodium Caprae Infection In Goats And Mosquito Species, Hoang Lan Anh Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ungulate malaria parasites, including Plasmodium caprae that infects the domestic goats, have received little attention. There are only few studies about the prevalence and mitochondrial genome analyses. This study, therefore, aimed to investigate the natural infection dynamics of P. caprae and its mosquito vectors. Besides, genome analyses based on nuclear genes of P. caprae and other related haemosporidian parasites were also carried out to obtain an in depth understanding about their evolutionary history and origin. A total of 423 goat blood samples were collected during rainy seasons from 2018 to 2021 to assess the malaria infection status. In addition, 1,019 …
Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model,
2022
Faculty of Veterinary Science
Anti-Proliferative And Apoptotic Effect Of Cannabinoids On Human Pancreatic Ductal Adenocarcinoma Xenograft In Balb/C Nude Mice Model, Quang Trung Le
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human pancreatic ductal adenocarcinoma (PDAC) is a highly malignant and lethal tumor of the exocrine pancreas. Cannabinoids extracted from the hemp plant Cannabis sativa have been suggested as a cancer therapy due to their anti-tumor effects on several human tumors. However, the anti-tumor effect of cannabinoids on human PDAC is still understudied. Therefore, the objective of the current study is to study the inhibition of proliferation and the induction of apoptosis potencies of cannabinoids in PDAC xenograft nude mice model. The human PDAC cell line (Capan-2) was subcutaneously injected into twenty-five nude mice. After tumor reached 200 mm3 in volume, …
In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis,
2022
Faculty of Veterinary Science
In Vitro Inhibitory Effects Of Turmeric Extracts Against Biofilm Formation And Virulence Of Flavobacterium Oreochromis, Sirawich Ngernson
Chulalongkorn University Theses and Dissertations (Chula ETD)
Flavobacterium oreochromis is an opportunistic bacteria and causative agent of columnaris disease that affects freshwater fish worldwide. To initiate infection, the bacteria are required to attach and form biofilm on the fish's skin. The bacteria cells in the biofilm then produce toxins that can degrade fish connective tissue, resulting in lesions and ulcers. This study aimed to evaluate the efficacy of turmeric (Curcuma longa L.) extract by three organic solvents (hexane, ethanol, and methanol) in biofilm inhibition and modulation of biofilm-associated genes of F. oreochromis. Turmeric extracts by three different organic solvents were tested for their ability to inhibit the …
Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand,
2022
Faculty of Veterinary Science
Molecular Characterization Of Clinostomum Spp. Infecting Cultured Snakeskin Gourami (Trichopodus Pectoralis) In Thailand, Sk Injamamul Islam
Chulalongkorn University Theses and Dissertations (Chula ETD)
The digeanean Clinostomum spp. is a significant parasitic pathogen of freshwater fish. It has low host specificity and a wide distribution geographically. Morphological and molecular data analyses have been performed on Clinostomum to assess its diversity. However, the molecular occurrence of Clinostomum spp. in aquaculture in Thailand has yet to be reported. Snakeskin gourami (Trichopodus pectoralis), a freshwater fish cultured in Thailand, has significant economic importance. Herein, the study focuses on describing a digenean Clinostomum in the abdominal cavity of T. pectoralis and reports the prevalence of this parasite from central Thailand. Two hundred and sixty-two 4 to 8- month-old …
Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022,
2022
Faculty of Veterinary Science
Seroprevalence Of Lawsonia Intracellularis Antibody Among Swine Herds In Thailand, December 2021-March 2022, Thanh Nguyen Che
Chulalongkorn University Theses and Dissertations (Chula ETD)
The gastrointestinal infectious diseases caused by bacteria have been a major problem in the Thai swine industry. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis. Porcine ileitis has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied for determining pathogen-antibodies seroprevalence and the timing of L. intracellularis infection when introduced to the herd. A lack of serological data since 2009, as well as recent antibiotic usage restrictions, may have a negative impact on porcine ileitis seroprevalence in Thailand. …
Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks,
2022
Faculty of Veterinary Science
Transferable R Plasmids In Escherichia Coli Isolated From Meat Ducks, Zulqarnain Baqar
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to describe the AMR characteristics and transferable R plasmids in Escherichia coli isolated from meat ducks reared in an open house system. One hundred seventy-seven (n=177) commensal E. coli were previously isolated from duck cloacal swabs. In this study, all were biochemically confirmed and examined for their susceptibilities to 15 antimicrobial agents by broth microdilution method. Transfer of R plasmids was tested by biparental mating method followed by plasmid replicon typing (PBRT) and plasmid multi-locus sequence typing (pMLST). The highest resistance rates were observed for ampicillin (83.0%) and tetracycline (81.9%) while multidrug resistance was common (86.4%). Extended-spectrum …
Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand,
2022
Faculty of Veterinary Science
Phenotypic And Molecular Characterization Of Streptococcus Agalactiae Causing Bovine Mastitis In Central Region Of Thailand, Sirirat Wataradee
Chulalongkorn University Theses and Dissertations (Chula ETD)
Streptococcus agalactiae is a highly contagious pathogen with a significant economic impact on bovine mastitis. The research aims to achieve three objectives: 1) identifying and characterizing S. agalactiae strains responsible for bovine mastitis based on their phenotypic and genotypic characteristics, 2) determining susceptibility profiles to antimicrobial agents, and 3) applying molecular epidemiological analysis to explore intramammary infections in Thai dairy herds. We analyzed a collection of one hundred S. agalactiae isolates from clinical and subclinical mastitis cases in thirteen dairy herds in central region of Thailand. We used conventional microbiological procedures, the VITEK® 2 system, and molecular detection of the …
Influence Of Cataract On Electroretinal Responses In Small Dog Breeds,
2022
Faculty of Veterinary Science
Influence Of Cataract On Electroretinal Responses In Small Dog Breeds, Chayada Noosen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine cataract is a condition of lens opacity that if severe, can lead to vision loss. High prevalence of cataract is evident in small dog breeds. To assess retinal function in cataract patients, flash electroretinography is commonly used in practice. This study aimed to compare time- and frequency-domain electroretinal responses between normal and cataract eyes in small dog breeds. Eighty-four eyes from small dog breeds categorized into immature cataract (n=49), mature cataract (n=25) and control (n=10) eyes in this study. Full-field flash intensity series ERG was performed in dogs under scotopic and photopic condition. Data was analyzed and compared among …
Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin,
2022
Faculty of Veterinary Science
Porcine Semen Microbiota Analysis In Relation To Sperm Quality In Semen Extender Coupled With Gentamicin, Cong Bang Ngo
Chulalongkorn University Theses and Dissertations (Chula ETD)
The present study was carried out to (i) investigate the association between boar semen microbiota and semen qualities and (ii) determine the relationship between boar bacteria contamination and longevity of boar semen preserved for 3 days in BTS with and without gentamicin. A total of 20 Duroc ejaculates were collected from standard quality boars (n=10) and high-quality ones (n= 10). Besides 3 ml of fresh semen of each ejaculate was subjected to 16S rRNA gene sequencing and bioinformatics including alpha diversity, beta diversity, and taxonomic mapping, each ejaculate was diluted in two conditions of Beltsvile Thawing Solution (BTS) extender: CONTROL: …
The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models,
2022
Faculty of Veterinary Science
The Effect Of Platelet-Rich Plasma And Hyaluronic Acid Nanogel On Wound Healing In Rat Skin Models, Pirada Virankabutra
Chulalongkorn University Theses and Dissertations (Chula ETD)
The study aimed to validate two-step procedure PRP protocol and assess the effect on skin healing process in rats treated with PRP and/or HA nanogel. A total of 46 Wistar rats were enrolled in the study. PRPwas produced and its hematological parameters were examined. Six skin defects were created and topicals (control, PRP, HA, PRP/HA, PRP/HA cream, and cream) were applied to the wounds. The progression of wound reduction was photographed daily, and calcutated to wound area. Skin samples were collected according to sampling timepoints: day 0, 3, 7,10, and 14, and stained for histological grading. The platelet (2457.15 $ …
Interaction Effects Of Age And Body Weight Losses During Moulting On The Performance Ofcommercial Laying Hens,
2022
TÜBİTAK
Interaction Effects Of Age And Body Weight Losses During Moulting On The Performance Ofcommercial Laying Hens, Mian Mubashar Saleem, Jibran Hussain, Usman Elahi, Ehsaan Ullah Khan, Muhammad Usman, Syed Ghulam Mohayudin Hashmi, Sohail Ahmad
Turkish Journal of Veterinary & Animal Sciences
This study evaluated the interaction effects of different age groups and body weight losses during moulting on the productive performance, egg quality, and immune response of commercial laying hens. In total 324 Lohmann Selected Leghorn Lite laying hens of 80 and 108 weeks old were studied. A completely randomized experimental design in factorial arrangement, with 6 treatments of 6 replicates with 9 hens each, was applied. Treatments consisted of 2 production cycles (80 and 108 weeks) and three body weight losses during moulting (20%, 25%, and 30%). Productive performance (body weight, feed intake, egg weight, production percent, egg mass, feed …
Molecular Characterization Of Bacillus Anthracis Isolates Recovered From Nomic Andnonnomic Hosts,
2022
TÜBİTAK
Molecular Characterization Of Bacillus Anthracis Isolates Recovered From Nomic Andnonnomic Hosts, Doğan Akça, Fati̇h Büyük, Emre Karakaya, Mustafa Reha Coşkun, Eli̇f Çeli̇k, Mi̇tat Şahi̇n, Özgür Çelebi̇, Sali̇h Otlu, Ali̇ye Gülmez Sağlam, Eray Büyük, Seda Durhan, İzzet Burçi̇n Saticioğlu, Seçi̇l Abay, Tuba Kayman, Fuat Aydin
Turkish Journal of Veterinary & Animal Sciences
Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. …
Determination Of Plant-Specific Retrotransposons In Chicken,
2022
TÜBİTAK
Determination Of Plant-Specific Retrotransposons In Chicken, Levent Mercan, Ci̇hat Erdem Bülbül, Fati̇h Bi̇lgi̇, Sevgi̇ Marakli
Turkish Journal of Veterinary & Animal Sciences
Mobile genetic elements are also known as transposons, comprising a significant proportion of plant and animal genomes. Retrotransposons, a class of transposons, move copy and paste mechanism and, therefore, cause genome obesity. Moreover, the transferability of retrotransposons within and between different kingdoms has provided valuable information for evolutionary relationships in addition to detailed knowledge about genomes. We identified barley-specific retrotransposons (Nikita and Sukkula) and determined polymorphism ratios of these retrotransposons in a chicken genome for the first time to gain new insights for retrotransposons found in different genomes. Transposon sequences belonging to chicken and barley genomes were also investigated to …
Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey,
2022
TÜBİTAK
Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey, Şahi̇n Çakir, Murat Yildirim, Kadi̇r Serdar Di̇ker, Fevzi̇ye İpek Keski̇n, Selcen Yüksel, Burak Deveci̇, Erhan Akçay
Turkish Journal of Veterinary & Animal Sciences
A national epidemiological research project was carried out to define the dynamics affecting the epidemiology of bovine tuberculosis (bTB) infection in Turkey and to identify the risk factors. Official veterinarian (OV) and breeder original questionnaires were produced separately as part of this study to collect thorough data regarding the disease from the field. The number of questionnaires that needed to be filled out was decided by 95% confidence interval (CI) and 5% margin of error. The findings of 371 OV and 317 breeder questionnaires completed online across the country were analyzed. In addition, 28 outbreaks determined by random method were …
Estimation Of The Factors Affecting Lactation Milk Yield Of Holstein Cattle By The Adaptiveneuro-Fuzzy Inference System,
2022
TÜBİTAK
Estimation Of The Factors Affecting Lactation Milk Yield Of Holstein Cattle By The Adaptiveneuro-Fuzzy Inference System, Aycan Mutlu Yağanoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study is to estimate lactation milk yield of Holstein cattle using first calving age, lactation period, and service period with (ANFIS) adaptive neuro-fuzzy inference system. The input variables for the system in the study were first calving age, lactation period, and service period. The output variable from the system was lactation milk yield. Predicted values obtained from the ANFIS were compared with the observed values. Twenty-seven rule functions are used to obtain the best model and 1000 epochs are used to estimate the accuracy of the training and testing error. The relations between the output and …
The Effects Of Rations Containing Ad Libitum Limestone Grit With Whole Flaxseed On Quails?Performance, Egg Quality, And Blood Parameters, Kadi̇r Emre Buğdayci, Fatma Karakaş Oğuz, Mustafa Numan Oğuz, Cafer Mercan
Turkish Journal of Veterinary & Animal Sciences
The purpose of this research is to determine the effects of consuming feed rations containing ad libitum limestone grit with whole or ground flaxseed (10%) on quails? performance, egg quality (shape index, albumen index, yolk index, and Haugh unit), and some blood parameters (hematocrit, cholesterol, and triglyceride) during a period of 8 weeks. Five weeks old quails (Coturnix coturnix japonica; 140 in total) were divided into control and 4 trial groups. The rations of the first (W) and second (G) trial groups consisted of whole or ground flaxseeds (10%), respectively. The composition of the rations of the third (Wg) and …
