Veterinary Medicine Commons^™

Identifying Bisphosphonate Protein Biomarkers In Equine Sera Using Mass Spectrometry Methods, Malinda Jayne Alison Porter

Theses and Dissertations--Veterinary Science

Bone resorptive diseases affect humans and horses, alike. Examples of these diseases include osteoporosis, Paget’s disease, and osteogenesis imperfecta in humans, and navicular disease in the horse (Mitchell et al., 2019; Suva et al., 2021). Bisphosphonates are used to treat these diseases, and may remain in the bone for several years after administration, demonstrating the need for newer drug testing methods.

The purpose of the discovery phase of this study was to use blood samples of in-training horses which had been given an initial administration of the bisphosphonate tiludronate and identify protein biomarkers that changed in response to this class …

The Effect Of Intra-Articular Corticosteroids On The Systemic Messenger Rna Response In An Equine Experimental Inflammation Model, Emma Elaine Partridge

Theses and Dissertations--Veterinary Science

Intra-articular (IA) corticosteroids are regularly used in equine athletes for the control of joint inflammation. The goal of this study was to utilize an acute synovitis inflammation model to determine the residual effects of IA betamethasone and triamcinolone acetonide on various inflammatory parameters and lameness.

Five mixed-breed, 2-year-old horses were randomly allocated to an IA treatment of the radiocarpal joint with 9 mg of either betamethasone or triamcinolone acetonide. Two weeks following treatment, horses were injected with 1μg of lipopolysaccharide (LPS) diluted in 1 mL of 0.9% sterile saline. Following LPS injection, horses were crossed-over and both sets of injections …

The Effects Of Cannabidiol On Senior Equine Health, Shelley Turner

Theses and Dissertations--Veterinary Science

Cannabis sativa L., has been revealed to produce hundreds of phytocannabinoids, of which cannabidiol (CBD) is one of the most desired. It has been revealed that CBD can potentially treat inflammation and act as an analgesic in humans without psychoactive effects of delta-9-tetrahydrocannabinol (THC). Recently, there has been interest in understanding the potential health benefits of CBD for horses. With an increasing senior horse population (< 15 years old), alternatives to the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as phenylbutazone, firocoxib, and flunixin meglumine, are desired as these common anti-inflammatory treatments have negative side effects. Because senior horses may have chronic low-grade inflammation, termed inflamm-aging, they are a natural model to investigate the efficacy of CBD on inflammatory responses as well as other health parameters. Given this interest, and due to the lack of research conducted on CBD in the horse, the overall objective of this dissertation work was to conduct both basic and applied research investigating the effects of CBD on equine immune function and health. The first objective was to determine the in vitro effects of CBD as an anti-inflammatory. Peripheral blood mononuclear cells (PBMCs) from senior horses were cultured in vitro with increasing concentrations of pure CBD dissolved in dimethyl sulfoxide (DMSO). These cells were …

Accuracy Of A Sacral Drilling With A Custom 3d Printed Drilling Guide Or Free-Hand Technique In Canine Experimental Sacroiliac Luxation, Daniel Mccarthy

LSU Master's Theses

ABSTRACT

The objective of this study was to improve the accuracy of drilling during the repair of sacroiliac luxation with a 3D-printed patient-specific drill guide (3D-GDT) compared to free-hand drilling technique (FHDT). A blinded, randomized, prospective study was performed including sixteen canine cadavers (20-25 kg) euthanized for reasons not related to the study. Dorsal, bilateral, sacroiliac luxations (SILs) were created experimentally. A pelvic CT was performed pre- and post-drilling. The FHDT was drilled followed by 3D-GDT for each sacrum. CT and 3D measurements of craniocaudal and dorsoventral angles were compared between FHDT and 3D-GDT, as well as deviations of entry …

Growing South Dakota (Fall 2021 / Winter 2022), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 SDSU Launches New Professional Program in Veterinary Medicine
[Page] 7 The 6th Generation
[Page] 9 First-Gen Proud
[Page] 11 Student Success
[Page] 21 Alumni News
[Page] 27 CAFES News and Updates
[Page] 45 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Quality Parameters Of Wet And Dry Aged Beef Loins From Cattle Fed High Doses Of Vitamin E, David M. Velazco, Joe A. Sonderman, Nicolas J. Herrera, Chris Calkins

Nebraska Beef Cattle Reports

The objective of this experiment was to determine if dietary supplementation of high doses of vitamin E (alpha-tocopherol; 2,200 IU per day for 100 days) can impact quality attributes of wet and dry-aged beef strip loins. Steaks from beef cattle supplemented with high doses of vitamin E exhibited less lipid oxidation after wet or dry aging, took longer time to discolor during retail display, and sustained redder color for a longer period under retail display conditions compared to controls. Free amino acids related to positive beef flavor attributes were higher for dry-aged loins compared to traditional wet aged samples loins. …

Beef Quality And Oxidative Stability From Cattle Fed High Levels Of Vitamin E, Nicolas J. Herrera, Joe A. Sonderman, David M. Velazco, Chris Calkins

Nebraska Beef Cattle Reports

Meat color is a major factor for consumer meat purchasing decisions. Aging beef, which can improve tenderness, has been shown to accelerate discoloration in fresh beef, shortening retail display time, and generating negative flavor attributes. The objective of this study was to evaluate supplementing cattle high levels (2,200 International Units/ day) of Vitamin E to sustain meat quality during prolonged retail display in beef strip loins after 2 or 14 days aging compared to commercially-produced loins selected as controls. Results showed a treatment x age effect for Warner-Bratzler shear force and free calcium content, primarily due to aging. A dietary …

Effect Of Feeding Cars On Digestibility And Fatty Acid Flow In Finishing Cattle Diets, John C. Gibbons, Mitchell M. Norman, Tyler J. Spore, Hannah C. Wilson, Kylie Butterfield, Andea K. Watson, Galen E. Erickson, Jonathan W. Wilson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the impact of a liquid feed, Condensed Algal Residue Solubles (CARS) on diet digestibility and fatty acid flow to the small intestine. Three treatments included CARS at 0, 2.5, and 5% of the diet dry matter, replacing steam-flaked corn. The base diet included 73, 70.5, or 68% steam flaked corn, 15% dried distillers grains plus solubles, 8% alfalfa haylage, and 4% supplement. Inclusion of CARS in the diet did not affect dry matter or organic matter intake or digestibility of dry matter, organic matter or neutral detergent fiber. Amount of fatty acid flowing to …

Evaluation Of Green Grass On Nutrient Digestibility And Fatty Acid Flow In Cattle Finishing Diets, Aksel R. Wiseman, Tyler J. Spore, Mitchell M. Norman, Hannah C. Wilson, J. C. Macdonald, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

Six ruminally and duodenally cannulated steers were utilized in a 3 × 3 replicated Latin square experiment to test 0, 15, and 30% dietary inclusion of Green Grass (Sunseo Omega 3; Chungcheong Duk-Do, South Korea), a feed comprised of sesame meal, giant kelp, cassava, and sorghum (not currently approved to be fed in the US). Dry matter and fiber intake increased linearly with increased Green Grass inclusion. Dry matter and organic matter digestibility were not affected by Green Grass inclusion. Concentration of saturated fatty acids at the duodenum were similar for Green Grass 0 and Green Grass 15 with a …

Effect Of Biochar As A Feedlot Pen Surface Amendment On Manure Nutrient Capture, J. L. Sperber, Braden C. Troyer, Mitchell M. Norman, Levi J. Mcphillips, Heather Nobert, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

Two mass balance experiments were conducted during winter and summer seasons to evaluate the effect of spreading biochar on the feedlot pen surface on manure nutrient retention. The winter experiment (Dec to June) evaluated three treatments including biochar spread to pen surface (approximately 54 lb of biochar per steer), hydrated lime spread to pen surface (cooperation with UNL Environmental Engineering; approximately 68 lb per steer) and negative control (no treatment applied). The summer experiment (June to Nov) evaluated biochar treatment (approximately 68 lb of biochar per steer) against negative control. Biochar utilized in winter and summer was unprocessed and sourced …

Interaction Of Sweet Bran Inclusion And Corn Processing Method In Beef Finishing Diets, Rebecca L. Sjostrand, Levi J. Mcphillips, Braden C. Troyer, Maggie Youngers, Rick Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

The effects of corn processing method and Sweet Bran concentration on finishing cattle performance and carcass characteristics were evaluated in steam-flaked corn or a blend of high-moisture and dry-rolled corn-based diets. Sweet Bran was included at 0, 20, or 40% of diet dry matter. When cattle were fed 0% Sweet Bran, feeding steam-flaked corn resulted in a 11.7% improvement in feed conversion and heavier hot-carcass weight compared to feeding high-moisture/ dry-rolled corn. As Sweet Bran increased in the diet, there was a linear improvement in feed conversion for high-moisture/dry-rolled corn-fed steers and no change in feed conversion for steam-flaked corn-fed …

Effect Of Rapeseed Inclusion In Late-Summer Planted Oat Pasture On Growing Performance Of Beef Steers, Devin A. Jakub, Hannah E. Riley, Kristin E. Hales, Steven D. Shackelford, Harvey C. Freetly, M. E. Drewnoski

Nebraska Beef Cattle Reports

An oat monoculture was planted in late summer at 100 lb/ac and compared to oats planted at 50 lb/ac with rapeseed included at 3 lb/ac. Seed cost of the oat monoculture ($20/ac) was greater than the mix ($15/ac). Initial forage yield was not affected by the inclusion of rapeseed with fall oat. Calf gain was significantly greater and cost of gain was significantly decreased when rapeseed was included due to the combination of greater gains and lesser seed cost. Including rapeseed in late summer planted oats may be beneficial for producers who want to graze growing calves in the fall.

Interaction Of Urea With Frequency And Amount Of Distillers Grains Supplementation On Growing Steer Rumen Digestion Parameters, Haley F. Linder, Joshua E. Sebade, Tyler J. Spore, M. E. Drewnoski, J. C. Macdonald

Nebraska Beef Cattle Reports

Ruminally cannulated steers were used in 8 × 6 row-column design with 8 animals and 6 periods. Treatment design was a 2 × 2 × 2 factorial, with factors including amount of supplementation, frequency of supplementation, and inclusion of urea. Hay dry matter intake was reduced by increased amount of supplementation and by decreased frequency of supplementation. Total VFA concentration did not differ among treatments. Rumen ammonia-N concentration was impacted by an interaction of amount of supplementation and inclusion of urea but there was no effect of supplementation frequency. In situ NDF disappearance did not differ between daily and alternate …

Comparison Of Partially Confined And Traditional Cow-Calf Systems, Zac E. Carlson, Levi J. Mcphillips, Galen E. Erickson, M. E. Drewnoski, J. C. Macdonald

Nebraska Beef Cattle Reports

Production of cow-calf pairs and grow/finish performance of calves from a partially-confined, fall-calving, intensive cow-calf system were compared to a traditional, spring-calving, extensive cow-calf system. Body condition was lower at breeding and weaning for the fall-calving alternative system compared to the traditional, spring-calving system. Conception, calving, and weaning rates were not different among treatments. Weaning weights were lower for the fall-calving alternative system than traditional, spring-calving system. Following weaning, calves were grown for 117 d and then subsequently finished to the same target fatness for both systems. In the grower phase, gain was greater and feed conversion improved for the …

Genetic And Genomic Architecture In Eight Strains Of The Laboratory Opossum Monodelphis Domestica, Xiao Xiong, Paul B. Samollow, Wenqi Cao, Richard Metz, Chao Zhang, Ana C. Leandro, John L. Vandeberg, Xu Wang

School of Medicine Publications

The gray short-tailed opossum (Monodelphis domestica) is an established laboratory-bred marsupial model for biomedical research. It is a critical species for comparative genomics research, providing the pivotal phylogenetic outgroup for studies of derived vs ancestral states of genomic/epigenomic characteristics for eutherian mammal lineages. To characterize the current genetic profile of this laboratory marsupial, we examined 79 individuals from eight established laboratory strains. Double digest restriction site-associated DNA sequencing and whole-genome resequencing experiments were performed to investigate the genetic architecture in these strains. A total of 66,640 highquality single nucleotide polymorphisms (SNPs) were identified. We analyzed SNP density, average heterozygosity, nucleotide …

Effects Of Supplemented Intra- And Extra-Cellular Trehalose On Cryopreservation Of Feline Oocytes, Saengtawan Arayatham

Chulalongkorn University Theses and Dissertations (Chula ETD)

Study 1 aimed at examining the effects of different stages of oocyte maturation and trehalose concentrations on freezing ability and developmental capability of feline oocytes. The immature and matured feline oocytes were cryopreserved and thawed. They were assessed for developmental competence. The effects of different concentrations of trehalose (0, 0.125, 0.25, 0.5, 1 M) on the freezing ability of feline oocytes were tested. The cryopreservation generally induced poor embryo development, while immature oocytes were more susceptible to cryodamage compared with the matured oocytes (P<0.05). Incubation of oocytes with high concentrations of trehalose (0.5, 1 M) significantly reduced the developmental competence. Optimal concentrations of trehalose during cryopreservation of immature and matured oocytes were different (0.125 M and 0.25 M, respectively). It is concluded that stages of meiotic maturation affected the freezing ability of feline oocytes. Optimal concentration of trehalose was differently required for immature and matured feline oocytes. Study 2 aimed at examining the effect of membrane-permeable trehalose on the freezing ability of feline oocytes matured in vitro. intracellular trehalose (trehalose hexaacetate; Tre-(OAc)6) was synthesized from trehalose precursor and subjected to spectroscopic characterization. The membrane permeability of the Tre-(OAc)6 was investigated by incubating oocytes with different concentrations of Tre-(OAc)6 (3, 15, and 30 mM) and optimum concentration and the toxicity of Tre-(OAc)6 were subsequently assessed. The effects of Tre-(OAc)6 on freezing ability in terms of apoptotic gene expression and developmental competence of in-vitro matured oocytes were examined, respectively. The Tre-(OAc)6 permeated into the ooplasm of cat oocytes in a dose- and time-dependent manner. The highest concentration of intracellular trehalose was detected when the oocytes were incubated for 24 h with 30 mM Tre-(OAc)6. For the toxicity test, incubation of oocytes with 3 mM Tre-(OAc)6 for 24 h did not affect maturation rate and embryo development. However, high doses of Tre-(OAc)6 (15 and 30 mM) significantly reduced maturation and fertilization rates (P < 0.05). In addition, frozen-thawed oocytes treated with 3 mM Tre-(OAc)6 significantly upregulated anti-apoptotic (BCL-2) gene expression compared with the control (0 mM) and other Tre-(OAc)6 concentrations (15 and 30 mM). Oocyte maturation in the presence of 3 mM Tre-(OAc)6 prior to cryopreservation significantly improved oocyte developmental competence in terms of cleavage and blastocyst rates when compared with the control group (P < 0.05). Our results lead us to infer that increasing the levels of intracellular trehalose by Tre-(OAc)6 during oocyte maturation improves the freezing ability of feline oocytes, albeit at specific concentrations. Study 3 aimed at examining the effects of combination of intra-cellular (Tre-(OAc)6) and extra-cellular (α,α-trehalose) trehalose on freezing ability of feline oocytes. Optimal concentrations of Tre-(OAc)6 (0, 1.5, and 3 mM) and trehalose (0, 0.125, 0.25, and 0.5 M) were investigated for cryopreservation of matured feline oocytes. The combination of the two optimal concentrations were subsequently examined. The results showed that 3 mM of Tre-(OAc)6 improved post-thawed developmental competence, while extra-cellular trehalose at 0.25 M significant enhanced freezing ability of feline oocytes (P<0.05). The combination of intra- and extra-cellular trehalose significantly improved developmental capability of frozen-thawed matured feline oocytes compared to non-trehalose control.

Metaproteomic Analysis Of Gut Resistome In The Cecal Microbiota Of Fattening Pigs Raised Without Antibiotics, Pamornya Buthasane

Chulalongkorn University Theses and Dissertations (Chula ETD)

Improper use of antibiotics in swine could reduce commensal bacteria and possibly increase pathogen infections via the gut resistome. This study aimed to compare the metaproteomic profiles of gut resistome and related metabolism in the cecal microbiota of fattening pigs raised under antibiotic-free (ABF) conditions with those of ordinary industrial pigs (controls, CTRL). The top three relative abundant microbials in both groups were Escherichia coli, Ruminococcus and Lactobacillus, followed by Bacteroides and Bifidobacterium. E. coli, Lactobacillus and Bacteroides were found to be increased in the CTRL group, whereas Ruminococcus and Clostridium were greater in the ABF group. The highest abundance …

Experimental Elucidation Of The Life Cycle Of Drepanocephalus Spathans (Digenea: Echinostomatidae) With Notes On The Morphological Plasticity Of D. Spathans In The United States, Neely R. Alberson, Thomas G. Rosser, Tommy King, Ethan T. Woodyard, Lester Khoo, Wes A. Baumgartner, Daviod J. Wise, Linda M. Pote, Fred L. Cunningham, Matt J. Griffin

United States Department of Agriculture Wildlife Services: Staff Publications

The echinostomatid Drepanocephalus spathans (syn. Drepanocephalus auritus) parasitizes the doublecrested cormorant Phalacrocorax auritus. In North America, the marsh rams-horn snail Planorbella trivolvis and ghost rams-horn snail Biomphalaria havanensis serve as snail intermediate hosts, both of which inhabit catfish aquaculture ponds in the southeastern United States. Studies have demonstrated D. spathans exposure can be lethal to juvenile channel catfish Ictalurus punctatus. Two studies were undertaken to elucidate the life cycle of D. spathans to establish a developmental time line. In both studies, D. spathans cercariae collected from naturally infected P. trivolvis individuals were used to infect channel catfish …

Scavenging Vs Hunting Affects Behavioral Traits Of An Opportunistic Carnivore, Mitchell A. Parsons, Andrew Garcia, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Background. Human-induced changes to ecosystems transform the availability of resources to predators, including altering prey populations and increasing access to anthropogenic foods. Opportunistic predators are likely to respond to altered food resources by changing the proportion of food they hunt versus scavenge. These shifts in foraging behavior will affect species interactions through multiple pathways, including by changing other aspects of predator behavior such as boldness, innovation, and social structure. Methods. To understand how foraging behavior impacts predator behavior, we conducted a controlled experiment to simulate hunting by introducing a prey model to captive coyotes (Canis latrans) and compared their behavior …

Influence Of Landscape Attributes On Virginia Opossum Density, David A. Bernasconi, Wesley C. Dixon, Matthew T. Hamilton, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Olin E. Rhodes Jr., Guha Dharmarajan

United States Department of Agriculture Wildlife Services: Staff Publications

TheVirginia opossum (Didelphis virginiana), North America's only marsupial, has a range extending from southern Ontario, Canada, to the Yucatan Peninsula, Mexico, and from the Atlantic seaboard to the Pacific. Despite the Virginia opossum's taxonomic uniqueness in relation to other mammals in North America and rapidly expanding distribution, its ecology remains relatively understudied. Our poor understanding of the ecology of this important mesopredator is especially pronounced in the rural southeastern United States. Our goal was to estimate effects of habitat on opossum density within an extensive multiyear spatial capture‐recapture study. Additionally, we compared the results of this spatial capture‐recapture …