Molecular Detection Of Canine And Feline Hemotropic Mycoplasmas In Northern Vietnam,
2023
Faculty of Veterinary Science
Molecular Detection Of Canine And Feline Hemotropic Mycoplasmas In Northern Vietnam, Bui Thi Huyen Thuong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hemoplasma is commonly used to refer to hemotropic Mycoplasma bacterial species belonging to the genus Mycoplasma that can attach to the surface of red blood cells, potentially leading to hemolytic anemia. These organisms can infect a wide range of mammals, including companion animals and humans worldwide. Specifically for companion animals, two main hemoplasma species can infect dogs, and three major species can infect cats including Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum, and Candidatus Mycoplasma turicensis, respectively. Currently, there is a lack of research data regarding hemoplasma infections in dogs and cats in Vietnam. Therefore, this study …
Microencapsulation Of Lactobacillus Plantarum Mb001 And Its Probiotic Effect On Growth Performance And Gut Integrity Of Broile Rs,
2023
Faculty of Veterinary Science
Microencapsulation Of Lactobacillus Plantarum Mb001 And Its Probiotic Effect On Growth Performance And Gut Integrity Of Broile Rs, Sasi Vimon
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this work were 1) to study the preparation and characterization of LPMB001/AG-AL, 2) todetermine the characteristics, stability and viability of LPMB001/AG-AL against simulated gastrointestinal tract, high temperature and storage conditions and 3) To evaluate the supplementation of LPMB001/AG-AL on growth performance,ileal digestibility, intestinal morphology and microbiome in broiler chickens. The present work declared microcapsule networks based on polymer matrices (sodium alginate, AL and agar, AG) and charges under ionic crosslink aw emulsion to encapsulate probiotics for extensive stability in adverse conditions. The microcapsule was studied on physicochemicalcharacteristics, swelling efficiency as well as release pertormance and viability under …
Expression Of Proteins Mediating Pulmonary Hypertension In Dogs Affected With Degenerative Mitral Valve Disease,
2023
Faculty of Veterinary Science
Expression Of Proteins Mediating Pulmonary Hypertension In Dogs Affected With Degenerative Mitral Valve Disease, Siriwan Sakarin
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pulmonary hypertension (PH) is defined by an abnormal elevation in pressure within pulmonary vasculature, often associated with degenerative mitral valve disease (DMVD) in dogs. While histopathological changes in the pulmonary arteries, known as pulmonary arterial remodeling, have been documented in human patients with PH, these alterations in DMVD dogs with PH have not been published. Proteomics, a potent method for exploring the entire protein complement of a biological sample, has emerged as a valuable tool for identifying PH-specific proteins, potentially serving as biomarkers for diagnosis and enhancing understanding of PH pathogenesis in DMVD dogs. This study aimed to evaluate the …
Screening Of Lactate Utilizing Bacteria And Their Beneficial Properties To Improve Energy Balance, Production And Ruminal Fermentation In Crossbred Dairy Goats,
2023
Faculty of Veterinary Science
Screening Of Lactate Utilizing Bacteria And Their Beneficial Properties To Improve Energy Balance, Production And Ruminal Fermentation In Crossbred Dairy Goats, Saranporn Poothong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Sixty-three strains were isolated from ruminal fluid of crossbred dairy goats. Based on phenotype and 16S rRNA gene, sixteenbacterial groups were identified and two novel species of Selenomonas were purposed in this study. The strain mPRGC5 was purposed as Selenomonas runimis and the strain mPRGC8 was named Selenomonas caprae. The antimicrobial activity of all the strains was determined.Ligilactobacillus salivarius MP3 showed antibacterial activity against Cutibacterium acnes subsp. acnes DSM 1897' and Kocuria thizophila Mill. The antimicrobial substance was genomically identified as enterolysin A. Furtherniore, L. salivarius MP3 presented probiotic properties and the potential antimicrobial activity against pathogens. The fourteen strains …
Application Of Anti-Müllerian Hormone As An Indicator For Ovarian Reserve And Ovarian Response To Hormonal Stimulation In Domestic Cats And Wild Felids,
2023
Faculty of Veterinary Science
Application Of Anti-Müllerian Hormone As An Indicator For Ovarian Reserve And Ovarian Response To Hormonal Stimulation In Domestic Cats And Wild Felids, Sittat Chumsri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Serum anti-Müllerian hormone (AMH) has been utilized to predict ovarian reserve and select suitable candidates for natural mating and artificial insemination (AI) in domestic animals. However, research on serum AMH concentration as a fertility marker in feline species remains limited. This study aims to investigate: 1) the possibility of using serum AMH levels to determine the number of follicles in domestic cats; 2) the validation of the AMH Gen II ELISA for measuring serum AMH levels in fishing cats, leopard cats and clouded leopards; and 3) the relationship between serum AMH levels and ovarian responsiveness in clouded leopards. In Study …
Contraception In Free-Ranging Long-Tailed Macaques With Special Focus On Anesthesia Management And Development Of Nanobody Targeting Extracellular Domain Of Follicular Stimulating Hormone Receptors,
2023
Faculty of Veterinary Science
Contraception In Free-Ranging Long-Tailed Macaques With Special Focus On Anesthesia Management And Development Of Nanobody Targeting Extracellular Domain Of Follicular Stimulating Hormone Receptors, Pakpoom Navanukraw
Chulalongkorn University Theses and Dissertations (Chula ETD)
The overlapping presence of free-ranging long-tailed macaques (Macaca fascicularis) and humans in Thailand and ฆSoutheast Asia resulted in physical violence, ravaging and zoonotic disease transmission. To mitigate these detriments, the synergy of surgical and nonsurgical contraception is essential for imperative population control. This thesis aimed to: 1) enhance animal welfare and safety of macaques undergoing surgical contraception by applying multimodal anesthesia practices; 2) generate alternative non-surgical contraceptives against the extracellular domain (ECD) of follicular stimulating hormone receptors (FSHr) in long-tailed macaques; 3) formulate nano/microstructured microspheres containing anti-FSHr scFv for oral delivery and determine the pharmacokinetics and bioavailabilityIn Study 1, the …
De Novo Assembly Of The Reference Genome, Genetic Variations And Serum Proteomics Of Eld's Deer (Rucervus Eldii),
2023
Faculty of Veterinary Science
De Novo Assembly Of The Reference Genome, Genetic Variations And Serum Proteomics Of Eld's Deer (Rucervus Eldii), Vichayanee Pumpitakkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Eld’s deer is a conserved wildlife species in Thailand. Due to the limited population group in captivity, Eld’s deer is facing a crisis of low genetic diversity, particularly among the captive Siamese Eld’s deer (SED) subspecies. This study aimed to construct genomes for a SED and a Burmese Eld’s deer (BED) for the first time and use genome-wide single nucleotide polymorphisms (SNPs) to assess the genetic purity and inbreeding status of 35 SED and 49 BED with limited pedigree data. Results revealed that the 2 subspecies diverged approximately 1.26 million years ago. All SED are purebred, whereas a small proportion …
Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.),
2023
Faculty of Veterinary Science
Utilization Of Testosterone-Encapsulated Nanoparticles For Masculinization Of Red Tilapia (Oreochromis Sp.), Jakarwan Yostawonkul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia (Oreochromis sp.) is a freshwater fish that is highly popular for aquaculture in Thailand. To achieve high yields, good growth rates, and better feed conversion rates, 17-alpha-methyltestosterone are commonly used to induce male sex in farmed tilapia. However, it has been found in previous studies that these synthetic hormones have low water solubility and limited absorption capabilities, leading to excessive hormone use. In this research, lipid-based nanoparticles, a widely used drug delivery technology, were employed to enhance the efficiency of synthetic hormones with low water solubility. This improved the dispersion of hormones in water, enhanced their absorption in the …
Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer),
2023
Faculty of Veterinary Science
Genome Characterization And Development Of Inactivated Vaccine Against Scale Drop Disease Virus (Sddv) In Asian Sea Bass (Lates Calcarifer), Putita Chokmangmeepisarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Scale drop disease virus (SDDV) is a significant pathogen affecting Asian sea bass (Lates calcarifer) in Southeast Asia, causing scale drop syndrome (SDS) and resulting in high mortality and substantial economic losses in aquaculture. Recently, SDDV has been reported in Asian sea bass farms throughout the Asia-Pacific region including Thailand, Indonesia, Malaysia, Singapore, and China. This study employed metagenomic methods to analyze bacterial and viral communities in infected fish tissues from Southeast Asia, leading to the recovery and characterization of the complete genome of SDDV strain TH7_2019. The genome, comprising 131 kb with 134 putative ORFs, showed high amino acid …
Characterization Of Antimicrobial Resistance And Virulence Factors In Multidrug-Resistant Extraintestinal Pathogenic Escherichia Coli Isolated From Canine Urinary Tract, Reproductive Tract And Bloodstream Infections,
2023
Faculty of Veterinary Science
Characterization Of Antimicrobial Resistance And Virulence Factors In Multidrug-Resistant Extraintestinal Pathogenic Escherichia Coli Isolated From Canine Urinary Tract, Reproductive Tract And Bloodstream Infections, Parinya Sroithongkham
Chulalongkorn University Theses and Dissertations (Chula ETD)
Despite extensive characterization of uropathogenic Escherichia coli (UPEC) causing urinary tract infections (UTIs), the genetic background of non-urinary extraintestinal pathogenic E. coli (ExPEC) in dogs, such as those causing canine pyometra, prostatic abscesses (PAs), and bloodstream infections (BSIs), remains inadequately understood. This study characterized the virulence determinants of 101 E. coli isolates from canine pyometra (n=61), PAs (n=38), and BSIs (n=2). The analysis revealed a stronger association of pyometra strains with UPEC compared to PA strains. Forty-one isolates were resistant to third-generation cephalosporins and/or fluoroquinolones, with 14 being extended-spectrum β-lactamase producers and carrying blaCTX-M. Whole-genome sequencing was performed on 10 …
Development And Evaluation The Efficacy Of A Water-Soluble Probiotic Microencapsulation Prototype For Pediococcus Acidilactici Isolated From Broiler Chickens,
2023
Faculty of Veterinary Science
Development And Evaluation The Efficacy Of A Water-Soluble Probiotic Microencapsulation Prototype For Pediococcus Acidilactici Isolated From Broiler Chickens, Ratchnida Kamwa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Probiotics are live bacteria that can be given orally to promote gastrointestinal health. A limitation of probiotic use is their diminished efficiency following the loss of live cells during high-temperature processing and storage. Microencapsulation technology has been created to protect probiotic cells, preserve their viability, and maintain their efficacy following manufacture. The aim of this study was to evaluate the effectiveness of a water-soluble microencapsulation method using gum Arabic (GA) and skim milk (SKM) over a three-month storage period following processing. Four strains of Pediococcus acidilactici (BYF26, BYF20, BF9, and BF14) that were typical lactic acid bacteria (LAB) strains isolated …
Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex,
2023
Faculty of Veterinary Science
Novel Vaccination Strategy Against Vibrio Harveyi Infection In Asian Seabass (Lates Calcarifer) Using A Chitosan – Oxygen Nanobubble - Vaccine Complex, Nguyen Giang Thu Lan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vibriosis is a bacterial disease caused by Vibrio harveyi (V. harveyi; Vh ) that poses a substantial threat to Asian seabass (Lates calcarifer) aquaculture. In this study, we investigated alternative approaches to conventional injection vaccination for prevention of V. harveyi in Asian seabass. The first approach involved an immersion prime with heat-inactivated V. harveyi vaccine, followed by two oral booster doses (day 14-18 and day 28-32). Immunized fish demonstrated higher expression levels of immune genes (i.e. TNFα, IL1β, CD4, CD8, and IgM) following the immersion prime and the first oral booster dose, peaking at 14 and 28 days post vaccination …
Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds,
2023
Faculty of Veterinary Science
Farrowing Performances And Piglet Characteristics In Highly Prolific Sows In Tropical Environment: Impact Of Aging, Ambient Temperature And Humidity Within The Barn, And Feeds, Yosua Kristian Adi
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hyperprolific sows have been widely distributed in the global swine industry, including Thailand. Therefore, factors affecting the farrowing performance and piglet characteristics of these sows were investigated to establish effective management practices, especially in tropical climates. Moreover, strategies to enhance sow colostrum production and expedite the farrowing process were also explored. Additionally, deep insight into the effect of aging on farrowing performance, particularly farrowing duration, through investigation of reproductive organ morphology and oxytocin receptor expression on uterine tissue was conducted. The study revealed that sows with a high number of piglets born per litter, a high parity number, and parturition …
Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats,
2023
Faculty of Veterinary Science
Assessment Of Longitudinal Systolic Funcion By Using Tissue Motion Annular Displacement In Hypertrophic Cardiomyopathy Cats, Nadthagarn Glaewketgarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
In humans, tissue motion annular displacement (TMAD) providesresponsive and accurate assessment on longitudinal systolic function. The aim ofthis study is to investigate the LV longitudinal systolic function in hypertrophiccardiomyopathy (HCM) and control cats by using TMAD. The study includes 26control cats and 21 HCM cats. Global TMAD were not influence by breed, sex, age,and heart rate. Global TMAD and % Global TMAD significantly decreased in theHCM group (2.86±0.86 mm and 11.46±3.37%) when compared to control group(3.95±0.89 mm and 16.12±2.94%) (p<0.001 and <0.001), suggesting LV longitudinalsystolic dysfunction in HCM cats. LV fractional shortening (LVFS) showed nodifference between the control (52.76±11.63%) and HCM stage group(59.80±13.51%) (p=0.061). Indicate that TMAD is a sensitive technique foridentifying LV longitudinal systolic dysfunction. Global TMAD and % global TMADhad a significant correlation with global longitudinal strain (GLS). Therefore, TMADcan be used to evaluate longitudinal systolic function in HCM. The reliability test ofglobal TMAD and % global TMAD were considered moderate. In conclusion, globalTMAD and % global TMAD was significantly decreased in cats with HCM comparedwith the control group and was considered as sensitive and repeatable techniquefor evaluating LV longitudinal systolic dysfunction in cats with HCM
Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database,
2023
Faculty of Veterinary Science
Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database, Xiaoxian Su
Chulalongkorn University Theses and Dissertations (Chula ETD)
Milk production traits in dairy cattle, such as milk yield, fat content, and protein levels, are pivotal to the economic success of the dairy industry. These traits not only determine the quality and quantity of milk produced but also directly impact the profitability of dairy operations. Consequently, a deep understanding of the genetic architecture underlying these traits is essential for the development of effective breeding programs aimed at enhancing milk production and quality. The purpose of this study is to identify potential candidate genes related to milk production in Thai dairy cattle through systematic review of genome wide association study …
Safety Assessment Of Lactic Acid Bacteria (Lab) As Potential Probiotics For Thai Swine And Poultry: A Whole Genome Sequencing Approach,
2023
Faculty of Veterinary Science
Safety Assessment Of Lactic Acid Bacteria (Lab) As Potential Probiotics For Thai Swine And Poultry: A Whole Genome Sequencing Approach, Yuda Disastra
Chulalongkorn University Theses and Dissertations (Chula ETD)
The standard criteria for determining the safety of bacteria used as probiotics do not exist at this time. The objective of this investigation was to assess the feasibility and safety of eight lactic acid bacteria (LAB) strains employed as probiotics. Of these, four were Pediococcus acidilactici strains (IAF6519, IAF5919, AF2519, and AF2019), one Ligilactobacillus salivarius AF2319 derived from chickens and Pediococcus acidilactici P72N and Lactiplantibacillus plantarum L25F and L22F obtained from pigs. Phenotypic investigation was performed on biochemical features, antimicrobial resistance patterns, and hemolytic activity. P. acidilactici AF2519 and AF2019 strains were resistant to erythromycin and clindamycin, Ligilactobacillus salivarius strain …
Dynamic Community Of Strongyle Nematodes And Ivermectin Resistant Evidence Of Domesticated Horses In Thailand,
2023
Faculty of Veterinary Science
Dynamic Community Of Strongyle Nematodes And Ivermectin Resistant Evidence Of Domesticated Horses In Thailand, Mohamed Hossameldein Abdelfatah Mohamed Hamad
Chulalongkorn University Theses and Dissertations (Chula ETD)
Strongylids are a group of parasitic nematodes that infect horses and other equids worldwide, with a potential negative impact on their health and performance. There is no available research that has studied strongylids infection in domesticated horse populations nor assessed the effectiveness of ivermectin in treating these parasites in Thailand. This thesis, therefore, aimed to comprehensively investigate strongyle parasite intensity and factors influencing egg shedding patterns. Additionally, it utilized nemabiome metabarcoding approach to identify strongylid species, analyse ecological diversity, and assess co-occurrence patterns within the studied horse populations. Furthermore, it employed faecal egg count reduction (FECR) test to assess the …
Prioritization Of Candidate Genes For Mastitis Resistance Trait In Genome-Wide Association Studies (Gwas) Utilizing Multiple Methodologies In Thai Dairy Cattle,
2023
Faculty of Veterinary Science
Prioritization Of Candidate Genes For Mastitis Resistance Trait In Genome-Wide Association Studies (Gwas) Utilizing Multiple Methodologies In Thai Dairy Cattle, Nhu Le Uyen Vo
Chulalongkorn University Theses and Dissertations (Chula ETD)
Mastitis affects milk yield in dairy cattle worldwide, leading to economic losses. Recognized as a polygenic trait, mastitis resistance is influenced by genetic, environmental, and physiological factors. This study aimed to enhance the genetic architecture and selection of the candidate for mastitis resistance traits in Thai dairy cattle by several methods. The study consisted of two parts. In Part 1, the study is divided into 1A) collecting genes from a systematic review of published GWAS, 1B) Analyzing the gene list with the Thai dairy cattle whole genome sequencing database, and 1C) Performing pathway enrichment analysis to identify candidate genes. Further …
Reliability Of Cardiac Measurement By Vertebral Heart Score Among Different Thoracic Types Of Dogs,
2023
Faculty of Veterinary Science
Reliability Of Cardiac Measurement By Vertebral Heart Score Among Different Thoracic Types Of Dogs, Suphat Soeratanapant
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vertebral heart score (VHS) is a radiographic tool that widely use in veterinary clinical field for evaluating the heart sizeby measure a heart long and short axes and neutralize with vertebral length. However, VHSs among canine breeds are varied from thereference value. Thoracic type is one of the factors that affect to VHS because the shape of canine thoracic type is the structuralvariations that affect heart orientation. Subsequently, it affects to VHS value in thoracic radiographs. Computed tomography (CT) is anadvanced diagnostic tool that provide a multiplane image without superimposition effect. Therefore, CT provide more accurate heartorientation and its evaluation …
Impact Of Probiotic Supplementation In Lactating Sows On Immune And Oxidative Stress Biomarkers, Short-Chain Fatty Acids, Piglet Performance, And Fecal Microbiome,
2023
Faculty of Veterinary Science
Impact Of Probiotic Supplementation In Lactating Sows On Immune And Oxidative Stress Biomarkers, Short-Chain Fatty Acids, Piglet Performance, And Fecal Microbiome, Chutikan Srisang
Chulalongkorn University Theses and Dissertations (Chula ETD)
This clinical trial aimed to investigate the effects of probiotic supplementation in lactating sows on their serum immunoglobulin levels, milk yield, serum malondialdehyde (MDA), fecal short-chain fatty acid levels, piglet performance, and fecal microbiome. The research was conducted at a commercial swine breeding herd in central Thailand. The study included 109 Canadian Landrace × Yorkshire sows, with parity numbers ranging from 1 to 7. The sows were divided into two groups based on their parity numbers: control (n = 61) and treatment (n = 48). The control group sows received a conventional commercial lactation diet without any antibiotic supplementation, starting …
