Veterinary Medicine Commons^™

Accuracy Of Portable Human Glucose Meter (Accu-Chek® Performa) For Blood Glucose Measurement In Newborn Piglets, Morakot Nuntapaitoon, Sujin Sirisawadi, Sariya Asawakarn, Padet Tummaruk

The Thai Journal of Veterinary Medicine

Blood glucose concentration is a significant postnatal survival indicator in newborn piglets. The standard procedure is difficult to perform under field conditions and requires a long period of time for processing. Portable glucose meters have been invented to yield a faster result and utilize a small blood sample. The present study aims to compare glucose concentration mea s ur ed using a portable human glucose meter ( Accu- chek® Performa) with the standard protocol. A total of 200 blood samples were collected from the umbilical cord of newborn piglets and were measured using the portable human glucose meter immediately after …

Effect Of Dexmedetomidine Combined With Pethidine On Minimum Alveolar Concentration Of Isoflurane In Dogs, Kanjana Vinyunantakul, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

The effect of dexmedetomidine alone and when combined with pethidine on a minimum alveolar concentration (MAC) of isoflurane was determined in 24 client-owned adult male dogs before castration. Dogs were assigned to Group 1 (n = 12; dexmedetomidine, 5 µg/kg IM) or Group 2 (n = 12; dexmedetomidine, 5 µg/kgand pethidine, 5 mg/kg IM) . Sedation and response to noxious stimulation (digit clamping) were evaluated at 15 minutes after drug injection. Then, anaesthesia was induced via a face mask with isoflurane in oxygen and maintained via an endotracheal tube with an end tidal concentration of isoflurane (E´ISO%) of 1%, at …

Epidemiologic Evaluation Of Feline Urolithiasis In Thailand From 2010 To 2017, Vachira Hunprasit, Pinit Pusoonthornthum, Lori Koehler, Jody P. Lulich

The Thai Journal of Veterinary Medicine

This cross-sectional study describes the epidemiological data of feline urolithiasis in Thailand. Information on 923 feline uroliths submitted from Thailand to the Minnesota Urolith Center between January 2010 and December 2017 was evaluated. The frequency of urolith types, the relationships between urolith type and breeds and sex, were analyzed. Calcium oxalate was the most common urolith (49.5%) and was commonly found in male cats (OR = 1.81; 95%CI: 1.39, 2.36) with a mean age of 6.3±3.2 years. Struvite was the second most common urolith (38%), more frequently found in females (OR = 1.46; 95%CI: 1.11, 1.91) with a mean age …

Transcriptome Analysis Of Zi Geese (Anser Cygnoides) Prehierarchical Follicles And Verification Of Related Genes In Arginine Metabolic Pathway, Yongfeng Sun, Lulu Liu, Chang Liu, Cornelius Tlotliso Sello, Huiyan Wu, Hongtao Lu, Chenguang Xu, Yujian Sui

The Thai Journal of Veterinary Medicine

Arginine metabolism plays a pivotal role in the mammalian reproductive system by controlling various physiological processes of the reproductive organs including follicular development, ovulatory mechanisms and egg production. However little is known about the molecular mechanisms regulating arginine metabolism at early stages of folliculogenesis. The objective of the current study was to determine the arginine metabolic pathways in prehierarchical follicles of Zi geese through the Illumina Hiseq 2500 platform and their transcripts through the de novo assembly. In this study, five different stages of the geese prehierarchical follicles transcriptome were sequenced. A total of 31722729162 bp from 224929214 high quality …

Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi

The Thai Journal of Veterinary Medicine

This cross-sectional study aimed to determine the prevalence of Trypanosoma evansi infection and its associated risk factors in camels in the Jazan region, southwestern Saudi Arabia. A total of 679 blood samples were collected from three local breeds of camel – Awadia, Awarkia and Sawahelle – in 11 randomly selected governorates of Jazan region. Blood samples were screened by Giemsa-stained blood film (GSBF) and polymerase chain reaction (PCR) techniques. The overall prevalence of T. evansi was 30.9% (210/679, CI: 27.6–34.5%) by PCR, whereas a prevalence of 22.2% (151/679, CI: 19.3–25.5%) was revealed by GSBF. The highest prevalence was identified in …

Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho

The Thai Journal of Veterinary Medicine

The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …

Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the efficacy of the recombinant herpes virus of turkey-Newcastle disease virus (rHVT-NDV) vaccine against Newcastle disease virus (NDV) challenged in 14 days old broiler chickens. One hundred chickens were divided into 5 groups of 20 chickens each. Group 1 chickens received live NDV vaccine via the intra-nasal route. Group 2 chickens received rHVT-NDV vaccine by subcutaneous injection. Group 3 chickens, received both live NDV and rHVT-NDV vaccine. Groups 4 and 5 chickens did not receive any vaccine and served as positive and negative control groups. At 14 days old, all the chickens …

Combination Of Rock Inhibitor, Hypoxia And Melatonin Improved Differentiation Of Rabbit Induced Pluripotent Stem Cells Into Cardiac Progenitor Cells, Praopilas Phakdeedindan, Piyathip Setthawong, Mongkol Techakumphu, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Cardiac progenitors are a promising cell source for treating myocardial infarction. In this study, we improved the protocol for cardiac differentiation of rabbit induced pluripotent stem cells (iPSC) using Rho-associated protein kinase (ROCK) inhibitor (Y-27632), low O2 tension (hypoxia) and melatonin treatments. In experiment 1, the rabbit iPSCs were differentiated into cardiac cell fate via embryoid body (EB) formation with or without ROCK inhibitor. EB diameters were measured on day 3 of differentiation. In experiment 2, the EBs were plated on gelatin-coated dishes and further cultured in different oxygen tensions, hypoxia (5% oxygen) and normoxia (20% oxygen). The plated EBs …

Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun

The Thai Journal of Veterinary Medicine

Nicotine affected meiosis during oocyte maturation is associated with poor subsequent embryonic development. The aim of this study was to analyze the effects of nicotine exposure during in vitro maturation (IVM) on the outcomes of in vitro fertilization (IVF) and the expression of gene related development of oocytes and embryos. Immature (germinal vesicle, GV) oocytes were randomly cultured in IVM media containing nicotine at concentrations of 0.0 (control) 2.5, 5.0, and 10.0 mM. After culturing for 24 h, oocytes were assessed for their maturation rate, subjected to IVF and mRNA extraction for gene expression analysis using quantitative real time PCR …

Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the effect of a rapid heat-treatment method on Thai QX-like infectious bronchitis virus (IBV) and the efficacy on protection from the virus after heat treatment. QX-like IBV isolate THA46 was incubated at 56 oC. The incubated virus was collected every 5 min for up to 1 h and then they were inoculated into embryonated chicken eggs. At 6 days post-inoculation, allantoic fluid was harvested from the embryonated eggs which were inoculated with virus of the longest heat-treatment time that induced lesions in the embryos. That allantoic fluid was used in a subsequent …

Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang

The Thai Journal of Veterinary Medicine

Bovine mastitis has had a great impact on the economic losses of the dairy industry. Nowadays, environmental pathogens cause the main types of bovine mastitis, especially the streptococci. The aim of the study was to identify the major Streptococcus species causing bovine mastitis in Taiwan as well as to reveal their antimicrobial susceptibility profiles. By colony morphology identifying, Gram staining, and subculturing on Edwards agar, the preliminary identified streptococci were subjected to biochemical identification, latex agglutination tests and dick diffusion tests for susceptibility tests of 16 antimicrobial agents. Among a total of 157 strains, Streptococcus uberis was the most dominant …

Dual Infection Of A Thai Isolate Hp-Prrsv And The Pdmh1n1 2009 Siv In Weanling Pigs, Nattaphong Sangpratum, Roongtham Kedkovid, Yonlayong Woonwong, Chaitawat Sirisereewan, Sawang Kesdangsakonwut, Roongroje Thanawongnuwech, Jirapat Arunorat

The Thai Journal of Veterinary Medicine

After the introduction of the pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in Thai swine farms in 2009, a mild clinical outcome was observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in Thai swine farms, recently. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups; Negative, PRRSV, the SIV and Co-infection …

Surgical Removal And Recurrence Of Leiomyosarcoma In A King Cobra, Ophiophagus Hannah, Taksa Vasaruchapong, Panithi Laoungbua, Lawan Chanhome, Wijit Banlunara

The Thai Journal of Veterinary Medicine

An adult male wild-caught king cobra, Ophiophagus hannah, of unknown age was raised in captivity for seven years. The snake showed swelling at the caudal third of the body. Physical examination revealed an approximately 15x10 cm firm mass in coelomic cavity. Radiography showed soft tissue density causing complete obstruction of intestinal tract. The mass was surgically removed and diagnosed as leiomyosarcoma. The snake was monitored and the mass recurred two months post-surgery. A second surgical exploration showed severe adhesion of the mass to dorsal aorta, intercostal muscle, ribs and stomach. The snake was euthanized and the recurrent masses were diagnosed …

Use Of Plumage And Gular Pouch Color To Evaluate Condition Of Oil Spill Rehabilitated California Brown Pelicans (Pelecanus Occidentalis Californicus) Post-Release, Deborah L. Jaques, Kyra L. Mills, Barton G. Selby, Richard R. Veit, Michael H. Ziccardi

Publications and Research

Sublethal effects of oil spills may dampen seabird rehabilitation success due to lingering negative impacts of contamination and stress on reproduction and long-term survival. These effects can be difficult to measure while birds are in care as well as once birds are released. Expression of sexually selected traits that are sensitive to condition can provide information on physiological status of birds. We evaluated plumage molt and gular pouch skin color of California brown pelicans (Pelecanus occidentalis californicus) following oil contamination and rehabilitation to test for differences between previously oiled and rehabilitated (post-spill) and presumably uncontaminated pelicans. Post-spill pelicans released with …

Endoscopic Laser-Ablation For The Treatment Of Orthotopic And Ectopic Ureteroceles In Dogs: 13 Cases (2008-2017)., Cleo P. Rogatko, Allyson C. Berent, Larry G. Adams, Chick W. Weisse, Demetrius H. Bagley

Department of Urology Faculty Papers

BACKGROUND: Ureteroceles are a rare condition in dogs in which conventional treatments can result in substantial morbidity. Cystoscopic and fluoroscopic-guided laser ablation (CLA) of ureteroceles can successfully relieve obstruction.

OBJECTIVES: To describe the technique and outcomes of attempting CLA for treatment of ureteroceles in dogs.

ANIMALS: Thirteen client-owned dogs that underwent CLA for treatment of ureteroceles.

METHODS: Retrospective multicentered study. Medical records were reviewed in all dogs that underwent CLA for ureterocele(s). A laser was used to extend the opening of the ureteral orifice (UO) unless surgical conversion was necessary. Data collected included signalment, clinicopathologic data, imaging, procedural findings, complications, …

A Pilot Study Of The Effects Of Mycoplasma Ovipneumoniae Exposure On Domestic Lamb Growth And Performance, Thomas E. Besser, Jessica Levy, Melissa Ackerman, Danielle Nelson, Kezia R. Manlove, Kathleen A. Potter, Jan Busboom, Margaret Benson

Wildland Resources Faculty Publications

Mycoplasma ovipneumoniae is a globally distributed pathogen that has been associated with pneumonia in both domestic and wild Caprinae. It is closely related to M. hyopneumoniae, a respiratory pathogen of swine that is associated with decreased growth rates of pigs as well as clinical respiratory disease. In order to assess the effects of M. ovipneumoniae on lamb performance, we generated a cohort of lambs free of M. ovipneumoniae by segregation of test negative ewes after lambing, then compared the growth and carcass quality traits of M. ovipneumoniae-free and -colonized lambs from weaning to harvest. Some signs of respiratory disease …

Decrease In Population And Increase In Welfare Of Community Cats In A Twenty-Three Year Trap-Neuter-Return Program In Key Largo, Fl: The Orcat Program, Rachael Kreisler, Heather Cornell, Julie Levy

Management - TNR

The objective of this study was to evaluate the effect of a long-term (23-year) trap-neuter-return program on the population size of community cats in the Ocean Reef Community and to describe the demographic composition and outcome of enrolled cats. A retrospective study was performed using both cat census data collected between 1999 and 2013 as well as individual medical records for cats whose first visit occurred between 3/31/1995 and 12/31/2017. Medical record entries were reviewed to determine program inputs, cat outcomes, retroviral disease prevalence, and average age of first visit, sterilization, and death through 6/11/2018. Change over time was analyzed …

The Morphology And Evolution Of The Primate Brachial Plexus, Brian M. Shearer

Dissertations, Theses, and Capstone Projects

Primate evolutionary history is inexorably linked to the evolution of a broad array of locomotor adaptations that have facilitated the clade’s invasion of new niches. Researchers studying the evolution of primates and of their individual locomotor adaptations have traditionally relied on bony morphology – a practical choice given the virtual non-existence of any other type of tissue in the fossil record. However, this focus downplays the potential importance of the many other structures involved in locomotion, such as muscle, cartilage, and neural tissue, which may each be influenced by separate selective forces because of their different roles in facilitating movement. …

Animal-Assisted Interventions In Health Care Settings: A Best Practices Manual For Establishing New Programs: Volunteer Manual Template, Sandra B. Barker, Rebecca A. Vokes, Randolph T. Barker

AAI

This document accompanies Animal-Assisted Interventions in Health Care Settings: A Best Practices Manual for Establishing New Programs (Purdue Press, 2019) and serves as a template for readers to personalize for their facility. The generic terms “ABC Health Care Facility” and “AAI Program” are intended to be replaced by the names of the reader's facility and AAI program.

The book can be purchased from Purdue University Press.

Individual-Level Antibody Dynamics Reveal Potential Drivers Of Influenza A Seasonality In Wild Pig Populations, Kim M. Pepin, Kerri Pedersen, Xiu-Feng Wan, Fred L. Cunningham, Colleen T. Webb, Mark Q. Wilber

United States Department of Agriculture Wildlife Services: Staff Publications

Swine are important in the ecology of influenza A virus (IAV) globally. Understanding the ecological role of wild pigs in IAV ecology has been limited because surveillance in wild pigs is often for antibodies (serosurveillance) rather than IAVs, as in humans and domestic swine. As IAV antibodies can persist long after an infection, serosurveillance data are not necessarily indicative of current infection risk. However, antibody responses to IAV infections cause a predictable antibody response, thus time of infection can be inferred from antibody levels in serological samples, enabling identification of risk factors of infection at estimated times of infection. Recent …