Veterinary Medicine Commons^™

Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

A normalisationof targeted genes using the appropriate reference genes is necessary for reliable quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. This study aimed to investigate the appropriate reference genes and the numbers of the genes required for qRT-PCR in canine pulmonary arteries. Pulmonary arteries were collected from twenty-three dogs at necropsy. Candidate reference genesincluding ribosomal protein L32 (RPL32), ribosomal protein L13a (RPL13A), ribosomal protein S18 (RPS18), TATA box binding protein (TBP), beta-2-microglobulin (B2M), ribosomal protein S5 (RPS5), ribosomal protein S19 (RPS19) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pulmonary arteries were examined by qRT-PCR and analysed using four major algorithms, including …

Distribution And Risk Factors Of Clinical Caseous Lymphadenitis In Small-Holder Goat Herds In Northeastern Thailand, Suteekan Thongkwow, Natchanon Poosiripinyo, Nuttapon Pongkornkumpon, Sitang Saengsakchai, Nattakarn Klinkhiew, Tassapon Chalatan, Kwankate Kanistanon, Sawarin Lerk-U-Suke, Sarinya Rerkyusuke

The Thai Journal of Veterinary Medicine

Clinical caseouslymphadenitis was observed in 60 of 1,186 goats. A total of 34 small holder goat farms were investigated in 11 provinces in the northeastern part of Thailand. Approximately66.67%of clinical caseous lymphadenitis goats were infected with Corynebacterium pseudotuberculosis,as confirmed by an ELISA test or bacterial culture. The average prevalence of clinical caseous lymphadenitisin herds was 6.36+4.30%(4.86–7.86; 95%CI). The abscessation of a superficial lymph node was commonly found in proximal (46.67%), middle (38.33%), and distal (15%)parts of the body. The location of the lesion was significantly associated (p<0.05)with positivity either from ELISA or bacterial culture, as goats with lesionsinthe distal part of the body showed a higher positivity (90%)than at theproximal (75%)and middle parts(45%)(p=0.02), respectively. No significant difference was observed for goat gender, age, or breed in terms of infection prevalence or displays of clinical signs of caseous lymphadenitis(p>0.05). Controland prevention measures should incorporate client education on a number of factors, …

Occurrence Of Oral Shedding Of Retroperitoneal Fibromatosisassociated Herpesvirus And Lymphocryptovirus In Free-Ranging Macaques, Thailand, Supakarn Kaewchot, Chalisa Mongkolphan, Parut Suksai, Sunita Wingwon, Jira Niyomdham, Netnapa Vitithummakhun, Rattana Sariwongchan, Luxsana Prasittichai, Natanon Panpeth, Ladawan Sariya

The Thai Journal of Veterinary Medicine

Oropharyngeal swabs of 262 free-ranging macaques (Macaca fasicularisand M. mulatta) were tested for Herpesviridae. The occurrence was 24.4% (64/262), and no macaques showed clinical signs of the disease. The virus was restricted to the northern and north-eastern regions of Thailand. DNA polymerase genes of the 64 positive samples were then characterized and analyzed. Eight samples (3.1%) were found to be closely related to retroperitoneal fibromatosis-associated herpesvirus (RFHV) and were classified in the genus Rhadinovirus, subfamily Gammaherpesvirinae. The phylogram of the virus revealed it to be species-specific within the macaque population. A total of 56 samples (21.4%) exhibited a nucleotide relationship …

Efficacy Of Competitive Exclusion To Reduce Salmonella In Broiler Chickens, Nattaporn Jiratitipat, Prapasri Srikhong, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Competitive exclusion (CE) relies on the activity of normal flora to limit intestinal colonization by enteric pathogens. This study aims to investigate the effects of CEon Salmonellainfectionin broilers. The protective effect of CE on Salmonella cecal colonization was evaluated in 1 day-old chicks.In the laboratory trial, CE products were administered by oral inoculation, drinking water and whole body spray. Three days after treatment,the chickens were challenged with 107cfu/mL of SalmonellaEnteritidisorally, and were evaluated for 10 days. The CE-treated chickens showed comparable protection andthe number of Salmonella in the cecal contents significantly decreased(P<0.05)compared with those of the positive control. In the farm trial,CE was administeredby whole body spray at the hatchery and the second was administered by drinking water and evaluated at 32 and 42 days, respectively. The CE significantly reduced Salmonellacontamination in the farm trial andno Salmonellawas detected in the cecal contents compared with the control group. Average body weight gains, feed conversion ratioand theperformance indexof the chickens were greater than thecontrol group. Thus, the use of CEhad a positive effect on broiler performance. Furthermore, the CE-treated chickens displayed greater intestinal histology including villous height, submuscular layerand cell mitosis.These studies demonstrate that CEwas able to protect chickens from Salmonella cecalcolonization and it is apparent that new methodologies associated with the development of a workable CE program are needed in thepoultry industry.

Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Preferentially Infected The Apical Surface Of Primary Endometrial Cell Monolayer, Muttarin Lothong, Suphot Wattanaphansak, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

The underlying mechanism of porcine reproductive and respiratory syndrome virus (PRRSV) causing reproductive failure and re-circulation in herds has remained unclear. Endometrial cells primarily infected with PRRSV may serve as a significant target for PRRSV eradication. Primary endometrial (PE) cells from the porcine uterus were isolated and cultivated to pursue this possibility. Immunocytochemistry analysis revealed the protein expression of classical estrogen receptors (ER-and ER-), but not PRRSV receptors, CD163 and sialoadhesin in PE cells. PE cells were apically/basolaterally inoculated with PRRSV type I/type II isolated from PRRSV infected lungs or mock infection. Cytopathic effects (CPE) and PRRSV-GP5 positive cells were …

Successful Treatment Of Feline Pseudomycetoma Caused By Microsporum Canis: A Case Study, Pansawut Sudjaidee, Thanida Sananmuang, Kanchanarut Mankong, Premsak Jeeratanyasakul

The Thai Journal of Veterinary Medicine

A 3.5-year-old, 3.9 kg, intact, male Persian cat was presented at the hospital for chronic multifocal nodular dermatitis. The lesions with ulcerated nodules and yellow granular discharge were found mainly on the body and tail. Fungal culture was positive to Microsporum canisand skin biopsy revealed multifocal extensive fungal granuloma throughout necrotic fibrosis. The cat was treated with itaconazole for 4 months and the lesions were slightly improved. The skin nodules localized on the body, except the tail, were eventually removed. Approximately 1 month after surgery, the cat was vaccinated with inactivated vaccine against mycotic disease caused by Microsporum canistwo-week apart …

Variations In The Branching Pattern Of The Canine Coeliac Trunk In Thailand: An Anatomical Study, Rachanchai Chawangwongsanukun, Krai Meemon, Damri Darawiroj, Tuempong Wongtawan

The Thai Journal of Veterinary Medicine

Although the major dog population in Thailand is the Thai native dog, its anatomy has rarely been studied. This study aimed to investigate anatomical variations of the canine coeliac trunk (CT) in Thailand by observing 62 cadavers including 46 Thai native dogs and 16 non-Thai native dogs. All cadavers were preserved in formalin solution, and the arteries were illustrated by injection of red coloured latex. Variations of CT were initially classified into 2 main types and 7 subtypes (type 1a-e and 2a-b) basedon the previous reports. Type 1 was described as CT separated from the cranial mesenteric artery (CMA) while …

Effect Of Supplementing Magnesium Picolinate In Drinking Water On Growth Performance, Meat Quality And Cecal E. Coli Of Broiler Reared Under Tropical Conditions, Siriporn Namted, Choawit Rakangthong

The Thai Journal of Veterinary Medicine

The effect of Mg supplementation in drinking water was evaluated on the growth performance, carcass quality, meat quality and cecal E. colilevel of Broilersreared under Tropical Conditions. A sample of 192 male broiler chickens (Ross 308) were divided into 2 groups(each group consisting of 8 replicates of 12 chicks each) as: 1) control group, without Mg supplementation in the drinking water; and 2) Drinking Mg group, 10 mg of Mg/L was added into 1 L of their drinking water. It was shown that supplementing Mg in the drinking water significantly reduced body weight (P = 0.04) and tended to decrease …

Molecular Analysis Of Staphylococcus Aureus Isolated From Infected Dairy Goats, Muhammad Asghar Qazi, Shahid Sherzada, Abdul Wajid, Sonia Iqbal, Usman Atique, Rabia Bibi, Ayesha Maqbool, Saeed Akram Khan, Akhtar Ali, Tanveer Hussain, Masroor Elahi Babar

The Thai Journal of Veterinary Medicine

Staphylococcus aureusis the leading cause of mastitis in dairy goats and is globally recognized as a significant mastitis-causing pathogen in dairy animals. Previous studies have established it as the causative agent of various human disorders, including food poisoning and Staphylococcal enterotoxins. Furthermore, poor sanitary conditions suit infection by this bacterium. This study determined the occurrence of S. aureusin infected dairy goats by molecular analysis. A hundred raw milk samples were collected from two infected dairy goat breeds (Beetal = 71; Teddy = 29) and cultured on blood agar media. The strain identified as S. aureusby morphological method (Gram staining), biochemical …

Rubber Band Syndrome In A Dyspneic Dog, Nithida Boonwittaya, Saroch Kaewmanee

The Thai Journal of Veterinary Medicine

The rubber band syndrome has been reported inboth human and animal patients but the number of cases shows it is an uncommon condition. Most cases live in communities where a rubber band is commonly used for a wide variety of purposes. Here we report a case of a dyspneic dog with a linear constricting scar and a discharging sinus on the ventral neck. The radiography showed constructive signs. Surgical exploration found a colored rubber band burrowing into the tracheal lumen. Following surgery, the dog recovered without any complications in the postoperative follow-up period. This case emphasized that rubber band syndrome …

In Vitro And In Vivo Effect Of Chia Oil (Salvia Hispanica L.) On Haplorchis Taichui, Jiraporn Songsri, Danupol Ketthaisong, Thidarut Boonmars, Parichart Boueroy, Porntip Laummaunwai, Kulthida Vaeteewoottacharn, Panaratana Rattanasuwan, Chantana Boonyarat, Khomsorn Lomthaisong, Ratchada Tangwongchai, Glenn N. Borlace

The Thai Journal of Veterinary Medicine

Haplorchis taichuiis an intestinal heterophyidfluke that is pathogenic to humans. This parasite can mature in the small intestine of birds and mammals. The purpose of this study was to investigatethe effect of chia seed oil (Salvia hispanica L.) on H. taichuiin vitroand in vivo. The activity of adult H. taichuiworms incubated in Tyrode’s solution containing praziquantel (10μg/ml) or chia oil (1 or 10 mg/ml) for 1, 6, 12 and 24 hours was assessed by observation under a stereo-microscope. Syrian hamsters infected with 200 H. taichuimetacercariae were treated with 25mg/kgBW praziquantel in a single dose or 100mg/kgBW chia oil for 3 …

A Behavioral Study Of Stray Dogs In Temples In Nakhon Ratchasima Municipality, Thailand, Xin Huo, Panich Kumroptanasan, Pongchan Na-Lampang

The Thai Journal of Veterinary Medicine

The aim of thisstudy was to investigate the reactions of stray dogs in temples to strangers by means of a stranger-approach test.Behavioral data of temples dogs was collected from 5 temples from September to November 2018. The dogs’ behavior, social condition (alone, in pairs and with >3 dogs), reaction distances (0-2m, 2-5m and >5) and reaction behavior: neutral, avoidance, aggressive, vocalization, approachand tail movements were all observed. All the reaction behavior recorded was the dogs’initial reactions.After the 1ststranger-approach test, which was conducted in the 1stweek, the observer tried to get the dogs used to him by feeding and grooming them …

Male Burmese Pythons Follow Female Scent Trails And Show Sex-Specific Behaviors, Shannon A. Richard, Eric A. Tillman, John S. Humphrey, Michael L. Avery, M. Rockwell Parker

United States Department of Agriculture Wildlife Services: Staff Publications

Animals communicate with potential mates using species-specific signals, and pheromones are powerful sexual signals that modify conspecific behavior to facilitate mate location. Among the vertebrates, snakes are especially adept in mate searching via chemical trailing, which is particularly relevant given that many snake species are invasive outside their native ranges. Chemical signals used in mate choice are, thus, potentially valuable tools for management of invasive snake species. The Burmese python (Python bivittatus) is an invasive snake in the Florida Everglades where it is negatively impacting native fauna. In this study, we sought to: (i) determine if males can follow conspecific …

Variation In Host Home Range Size Decreases Rabies Vaccination Effectiveness By Increasing The Spatial Spread Of Rabies Virus, Katherine M. Mcclure, Amy T. Gilbert, Richard B. Chipman, Erin E. Rees, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Animal movement influences the spatial spread of directly transmitted wildlife disease through host-host contact structure. Wildlife disease hosts vary in home range- associated foraging and social behaviours, which may increase the spread and intensity of disease outbreaks. The consequences of variation in host home range movement and space use on wildlife disease dynamics are poorly understood, but could help to predict disease spread and determine more effective disease management strategies.
2. We developed a spatially explicit individual-based model to examine the effect of spatiotemporal variation in host home range size on the spatial spread rate, persistence and incidence of rabies virus …

Food Habits Of Coyotes (Canis Latrans) In The Valles Caldera National Preserve, New Mexico, Suzanne J. Gifford, Eric M. Gese, Robert R. Parmenter

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife managers needed to understand coyote (Canis latrans) ecology in order to develop management plans on the nascent Valles Caldera National Preserve in northern New Mexico. Managers concerned about low elk (Cervus elaphus) recruitment had observed an increase in sightings of coyotes and observations of coyote predation on elk calves. Our objective was to identify and quantify coyote diet, and assess the temporal variation in coyote diet on the Valles Caldera National Preserve, particularly as related to elk calf consumption. We examined coyote food habits using 1,385 scats analyzed monthly from May 2005 to November 2008. …

Clpc Affects The Intracellular Survival Capacity Of Staphylococcus Aureus In Non-Professional Phagocytic Cells, Gubesh Gunaratnam, Lorena Tuchscherr, Mohamed I. Elhawy, Ralph Bertram, Janina Eisenbeis, Christian Spengler, Thomas Tschernig, Bettina Löffler, Greg A. Somerville, Karin Jacobs, Mathias Herrmann, Markus Bischoff

School of Veterinary and Biomedical Sciences: Faculty Publications

Invasion and persistence of bacteria within host cells requires that they adapt to life in an intracellular environment. This adaptation induces bacterial stress through events such as phagocytosis and enhanced nutrient-restriction. During stress, bacteria synthesize a family of proteins known as heat shock proteins (HSPs) to facilitate adaptation and survival. Previously, we determined the Staphylococcus aureus HSP ClpC temporally alters bacterial metabolism and persistence. This led us to hypothesize that ClpC might alter intracellular survival. Inactivation of clpC in S. aureus strain DSM20231 significantly enhanced long-term intracellular survival in human epithelial (HaCaT) and endothelial (EA.hy926) cell lines, without markedly affecting …

Impact Of The Human Footprint On Anthropogenic Mortality Of North American Reptiles, Jacob E. Hill, Travis L. Devault, Jerrold L. Belant

United States Department of Agriculture Wildlife Services: Staff Publications

Human activities frequently result in reptile mortality, but how direct anthropogenic mortality compares to natural morality has not been thoroughly investigated. There has also been a limited examination of how anthropogenic reptile mortality changes as a function of the human footprint. We conducted a synthesis of causespecific North American reptile mortality studies based on telemetry, documenting 550 mortalities of known cause among 2461 monitored individuals in 57 studies. Overall 78% of mortality was the result of direct natural causes, whereas 22% was directly caused by humans. The single largest source of mortality was predation, accounting for 62% of mortality overall. …

Nursing Campus Therapy Dog, Deborah A. Hall

Nursing Theses and Dissertations

An acknowledged bond has existed between humans and animals throughout history. Therapeutic physical and psycho-social effects of these bonding relationships have been noted in health care settings. Professional nursing education is known to be one of the most demanding and stressful fields of study. Students begin to experience extreme stress early in their nursing education. An animal-assisted intervention with a therapy dog is an innovative and inexpensive action that can help decrease the stress, anxiety, and depression students experience in higher education.

The focus of this dissertation portfolio was an animal-assisted intervention with a nursing campus therapy dog. The initial …

Rabies Surveillance Identifies Potential Risk Corridors And Enables Management Evaluation, Amy J. Davis, Kathleen M. Nelson, Jordana D. Kirby, Ryan M. Wallace, Xiaoyue Ma, Kim M. Pepin, Richard B. Chipman, Amy Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

Intensive efforts are being made to eliminate the raccoon variant of rabies virus (RABV) from the eastern United States and Canada. The United States Department of Agriculture (USDA) Wildlife Services National Rabies Management Program has implemented enhanced rabies surveillance (ERS) to improve case detection across the extent of the raccoon oral rabies vaccination (ORV) management area. We evaluated ERS and public health surveillance data from 2006 to 2017 in three northeastern USA states using a dynamic occupancy modeling approach. Our objectives were to examine potential risk corridors for RABV incursion from the U.S. into Canada, evaluate the effectiveness of ORV …

Data Of Soil, Vegetation And Bird Species Found On Double-Crested Cormorant Colonies In The Southeastern United States, Leah Moran Veum, Brian S. Dorr, Katie Hanson-Dorr, R. J. Moore, Scott A. Rush

United States Department of Agriculture Wildlife Services: Staff Publications

This data article provides the methods and procedures followed to collect and analyse soil, vegetation and bird data on three different treatment islands in Guntersville Reservoir, Alabama. Samples were collected from randomly selected plot points from islands that were placed into three different treatment types: Colony (currently occupied by Double-crested Cormorants) (Phalacrocorax auritus; n 1⁄4 5), Historic (historically occupied by cormorants and currently abandoned; n 1⁄4 3) and Reference (never occupied by cormorants; n 1⁄4 4). We compared vegetation and tree metrics such as structure and diversity, as well as soil chemistry and bird diversity and communities among islands within …