Improved Virus Inhibition By Microencapsulated Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus,
2022
Chulalongkorn University
Improved Virus Inhibition By Microencapsulated Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus, Rachatapan Junchay, Patcharapong Thangsunan, Kittipat Supchukun, Jakarwan Yostawonkul, Sasithon Temisak, Kaewta Rattnapisit, Teerapong Yata, Waranyoo Phoolcharoen
The Thai Journal of Pharmaceutical Sciences
Porcine epidemic diarrhea virus (PEDV) has been affecting the swine industry, especially in suckling pigs with a high mortality rate. Among all the strategies to overcome PEDV, boosting mucosal immunity in the pig intestines through oral administration appears to be more efficient than other routes. However, there are biological obstacles such as an acidic environment that could damage biologics, a product from organisms often used for PEDV treatment. The plant-derived 2C10 monoclonal antibody (mAb) from Nicotiana benthamiana produced by transient expression was revealed as one of the potential candidates against PEDV through oral delivery. Herein, we demonstrated the calcium-alginate microencapsulation …
The Successful Treatment Of Brevundimonas Diminuta Using Ceftriaxone, In A Patient On Continuous Ambulatory Peritoneal Dialysis: A Case Report And Literature Review,
2022
Chulalongkorn University
The Successful Treatment Of Brevundimonas Diminuta Using Ceftriaxone, In A Patient On Continuous Ambulatory Peritoneal Dialysis: A Case Report And Literature Review, Sukrit Kanchanasurakit, Natnicha Woranithipokin, Piyakamol Teeranaew, Natthawee Kaewrattanapatthama, Chotirat Nakaranurack
The Thai Journal of Pharmaceutical Sciences
Brevundimonas diminuta (B. diminuta) is found in the healthcare environment and in the community. At present, there are few reports of infections caused by this microorganism, especially in patients undergoing continuous ambulatory peritoneal dialysis (CAPD). A 44-year-old woman undergoing CAPD was diagnosed with a B. diminuta infection. Ceftriaxone was given both intravenously and intraperitoneally. After treatment with ceftriaxone, the peritoneal dialysis fluid white cell count, neutrophil count, and symptoms improved. To the best of our knowledge, this is the first case report of a B. diminuta infection in a patient had undergoing CAPD in Thailand.
The Effect Of Pioglitazone On Phosphoproteome And Interactome Of Protein Phosphatase 2a In Primary Human Skeletal Muscle Cells,
2022
Wayne State University
The Effect Of Pioglitazone On Phosphoproteome And Interactome Of Protein Phosphatase 2a In Primary Human Skeletal Muscle Cells, Lana Alghanem
Wayne State University Dissertations
Skeletal muscle insulin resistance is one of the main contributors to Type 2 Diabetes (T2D), which is a chronic disease affecting 37.3 million people in United States (US), with an estimated annual cost exceeding $300 billion in the US. T2D is being treated with a variety of medications, including Pioglitazone (PIO), a potent insulin sensitizer for skeletal muscle. PIO is a synthetic ligand for the peroxisome proliferator-activated receptor (PPAR-γ), nuclear receptors regulating the expression of many genes involved in lipid and glucose metabolism. To date, the molecular action of PIO that sensitizes muscle to insulin is incompletely understood. Protein phosphorylation …
Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases,
2022
West Virginia University
Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases, Tasneem A. Arsiwala
Graduate Theses, Dissertations, and Problem Reports (ETD)
Transcranial low-intensity focused ultrasound is a unique technology to modulate the integrity of tight endothelial junctions and transiently increase BBB/BTB permeability to enhance therapeutic delivery. Despite promising early studies, present literature lacks agreement on key experimental conditions, which restricts our knowledge and the technique's widespread translation. This dissertation first provides a critical review of the current gaps in knowledge regarding the universal use of LiFUS in preclinical and clinical use. We then identify key parameters for translational and predictable opening of the BBB using a 3T MRI coupled with a clinical device. Our investigation highlights that passive permeability of the …
Aging, A Pathological Factor In Neurological Injury,
2022
West Virginia University
Aging, A Pathological Factor In Neurological Injury, Aruvi Vijikumar
Graduate Theses, Dissertations, and Problem Reports (ETD)
One of the main reasons for CNS drugs to fail in clinical development is not considering age as a risk factor while studying chronic age-related neurological/neurodegenerative diseases in preclinical studies. We first set out to gain a comprehensive understanding of the impact of age on various aspects (anatomical, immunological, and biochemical) in rodents that play a key role in determining the onset, progression, and evolution of disease severity. With advancing age, the vascular structure and function are compromised which is hypothesized to accelerate cognitive decline. The initial step toward developing novel therapeutics is to characterize the age-related vascular modifications. Utilizing …
Modulating Ketamine’S Locomotor Activating And Reinforcing Effects Through Drug Combinations,
2022
Virginia Commonwealth University
Modulating Ketamine’S Locomotor Activating And Reinforcing Effects Through Drug Combinations, Tegshjargal Baasansukh
Theses and Dissertations
Major Depressive Disorder (MDD) affects approximately 280 million people worldwide. The standard of care for pharmacological treatment of MDD has been monoaminergic antidepressants (MAA). However, MAAs have serious clinical limitations including a relatively slow onset of action and up to 40% of patients failing to respond and being diagnosed as “treatment resistant”. Ketamine, an N-methyl-D-aspartate antagonist, was originally approved for use as an injectable anesthetic. In addition, ketamine has been shown to rapidly improve depressive symptoms in patients with treatment resistant depression. In 2019, (S)-ketamine was approved as a nasal spray (Spravato®) for patients with MDD and treatment-resistant depression in …
Mechanisms And Treatment Of Pain-Depressed Behavior In Male And Female Mice,
2022
Virginia Commonwealth University
Mechanisms And Treatment Of Pain-Depressed Behavior In Male And Female Mice, Clare M. Diester
Theses and Dissertations
One of the largest discrepancies between preclinical and clinical assessment of pain and analgesia is the type of behavioral endpoint used. Although most preclinical research has historically focused on drug effectiveness to block reflexive withdrawal behaviors stimulated by a noxious stimulus, increasing evidence shows that drug-induced restoration of pain-depressed behaviors provides increased clinical translation with decreased susceptibility to false-positives. Accordingly, the first portion of this dissertation designed a behavioral battery that featured pain-depressed behaviors as well as more conventional pain-stimulated behaviors for testing candidate analgesics in male and female mice. The main findings are as follows. (1) Intraperitoneal injection of …
The Susceptibility Of Penicillin,
Ceftriaxone, And Cefotaxime
Against Streptococcus Pneumoniae
Isolated From Patients With Invasive
Pneumococcal Diseases At A Teaching
Hospital In Thailand,
2022
Chulalongkorn University
The Susceptibility Of Penicillin, Ceftriaxone, And Cefotaxime Against Streptococcus Pneumoniae Isolated From Patients With Invasive Pneumococcal Diseases At A Teaching Hospital In Thailand, Jantima Traipattanakul, Sudaluck Thunyaharn, Juthathip Suphanklang, Wichai Santimaleeworagun
The Thai Journal of Pharmaceutical Sciences
Objective: Pneumococcal disease is a severe invasive infection causing morbidity and death, so the appropriate antimicrobials are greatly important. We aimed to determine the minimum inhibitory concentration (MIC) of penicillin, cefotaxime and ceftriaxone against S. pneumoniae. Methods: The clinical isolates were obtained from patients with invasive pneumococcal disease admitted to Phramongkutklao Hospital, Thailand over a period of 10 years between 2006 and 2015. The in vitro antimicrobial susceptibility testing of antibiotics including penicillin, ceftriaxone and cefotaxime against S. pneumoniae isolates were determined by E-test. The MIC range, MIC50, and MIC90 (μg/ml) and percentage of susceptible isolates were recorded. Results: Forty …
Prevalence, Distribution, Treatment, And Modern Methods For In Vitro Diagnosis Of Alzheimer’S Disease In India: Challenges And Future Prospective,
2022
Chulalongkorn University
Prevalence, Distribution, Treatment, And Modern Methods For In Vitro Diagnosis Of Alzheimer’S Disease In India: Challenges And Future Prospective, Sopan Nangare, Pravin Patil
The Thai Journal of Pharmaceutical Sciences
In India, the plethora of evidence indicates that neurodegenerative conditions are a significant public health issue wherein over 4 million Indian peoples have been affected. For the past two decades, the prevalence of Alzheimer’s disease (AD) is growing rapidly in India that may due to the significant lack of health-care services and poor knowledge of AD and another form of dementia. Therefore, this in turn to develop ultramodern techniques for the efficient detection of AD biomarkers that helps to the prognosis and diagnosis of AD. Recently, significant progress has been observed in the area of AD that includes prognosis, and …
Gc-Ms Fingerprints Combined With Chemometric Analysis For The Authentication Of Morus Alba Leaves From Thailand,
2022
Chulalongkorn University
Gc-Ms Fingerprints Combined With Chemometric Analysis For The Authentication Of Morus Alba Leaves From Thailand, Malai Satiraphan, Aye Thida, Lawan Sratthaphut, Patcharawan Tanamatayarat, Onoomar Toyama
The Thai Journal of Pharmaceutical Sciences
Introduction: Morus alba L. is one of the economic plants in Thailand. Several varieties are grown in various parts of the country. Different varieties from different regions may produce dissimilar chemical fingerprints. Objectives: The gas chromatography coupled to mass spectrometry (GC-MS) fingerprints of 2 varieties of M. alba leaves from three locations in Thailand, along with tea products will be established. Materials and Methods: The GC-MS chromatograms combined with chemometric analysis were used to analyze the extracts of M. alba leaves and their tea products. Results: A total of 83 compounds comprising terpenes, saturated fatty acids, unsaturated fatty acids and …
Determination Of Sugars And Amino Acids In Japanese Wine Using Core-Shell Liquid Chromatography Tandem Electrochemical Detection,
2022
Chulalongkorn University
Determination Of Sugars And Amino Acids In Japanese Wine Using Core-Shell Liquid Chromatography Tandem Electrochemical Detection, Kana Yoshimura, Aiko Sano, Ryuichiro Suzuki, Shun-Ichi Mitomo, Yukiko Negishi, Toshiki Mutai, Florencio Arce Jr., Gerard Lee See, Takashi Tanikawa
The Thai Journal of Pharmaceutical Sciences
In this study, sugars (glucose, fructose, and sucrose) and amino acids in plum and yuzu wines were quantified with a new and simple method involving an electrochemical detector (ECD) and core-shell column [S-30/70=St (styrene)/DVB (divinylbenzene)-5TMDAH (tetramethyldiaminohexane)]. Analysis was conducted under the following conditions: A column temperature (40°C), flow rate (0.3 mL/min), injection volume (20 μL), and mobile phase (0.1 mol/L NaOH). Glucose, fructose, and sucrose had retention times of 14.68 min, 16.22 min, and 19.29 min, respectively, when measured using an ECD. When the sugar content was compared among the plum wines, plum wine A had the lowest sugar content. …
Effect Of Chronic Kidney Disease On Warfarin Responsiveness Among Thai Patients,
2022
Chulalongkorn University
Effect Of Chronic Kidney Disease On Warfarin Responsiveness Among Thai Patients, Sasimaporn Yaengkratok, Manat Pongchaidecha, Wichai Santimaleeworagun, Pornwalai Boonmuang
The Thai Journal of Pharmaceutical Sciences
This study aims to determine the effect of chronic kidney disease on warfarin response, including initial doses, maintenance doses, time in therapeutic range (TTR), and international normalized ratio (INR) variability. This is a retrospective cohort study at Rajavithi Hospital from 2015 to 2018. A total of 189 patients were included and divided into five groups based on estimated glomerular filtration rate (eGFR) (G1, eGFR ≥ 60 mL/min/1.73 m2; G2, 30–59 mL/min/1.73 m2; G3, 15–29 mL/min/1.73 m2; G4, ≤ 15 mL/min/1.73 m2; and G5, ≤ 15 mL/min/1.73 m2 with dialysis). The median age was 65.00 years, and 60.9% of patients were …
Behavioral Screening And Chiral Bioanalysis Of Emerging Stimulant-Type Drugs In Rats,
2022
Virginia Commonwealth University
Behavioral Screening And Chiral Bioanalysis Of Emerging Stimulant-Type Drugs In Rats, Tyson R. Baird
Theses and Dissertations
The epidemic of drug use in the United States and elsewhere in the world has resulted in tragic loss of life and substantial economic costs. Novel psychoactive substances (NPS) are one of the contributors to this problem, and the lack of information about many of these drugs compounds their risk. This dissertation proposes a strategy to use intracranial self-stimulation (ICSS) as a behavioral screening tool to assess emerging drugs of abuse for their abuse potential in order to generate a proactive threat assessment. A series of stimulant-type drugs including methcathinone, α-pyrrolidinohexanophenone (α-PHP), cocaine, and the phenyltropane analogs of cocaine WIN35428 …
The Effect Of Acute And Chronic Arsenic Exposure On Malignant Transformation Of The Human Bronchial Epithelial Cell (Beas-2b),
2022
Wayne State University
The Effect Of Acute And Chronic Arsenic Exposure On Malignant Transformation Of The Human Bronchial Epithelial Cell (Beas-2b), Bandar Almutairy
Wayne State University Dissertations
Arsenic (As3+), a metalloid abundant in the environment, is classified as a group I carcinogen associated with several common human cancers, including cancers in the lung, skin, bladder, liver, and prostate (Wei, Zhang & Tao, 2019b). The mechanisms of As3+-induced carcinogenesis had been extensively studied, and different mechanisms might be involved in various types of cancer (Wei, Zhang & Tao, 2019b). Recent studies showed that exposure to a high dose of arsenic is able to induce lung cancer. Moreover, arsenic activates oncogenic signaling pathways such as MAPKs, EGFR/RAS/RAF, PI3K/AKT, and JNK/STAT3 pathways as well as epigenetic alterations such as miRNAs …
The Benefits Of Astaxanthin To Improve Pain Relief In Patients With Painful Diabetic Neuropathy: An Open-Label, Randomized Controlled Trial,
2021
Faculty of Medicine, Duta Wacana Christian University, Yogyakarta, Indonesia
The Benefits Of Astaxanthin To Improve Pain Relief In Patients With Painful Diabetic Neuropathy: An Open-Label, Randomized Controlled Trial, Rizaldy Taslim Pinzon, Mary Rose Angelina Budi Harsana
Pharmaceutical Sciences and Research
Treatment of diabetic neuropathy is still carried out by providing symptomatic therapy, which only improves ± 50% of the total symptoms felt by patients, but does not tackle the underlying causes of the disease. Astaxanthin is a potent antioxidant, anti-inflammatory, and anti-diabetic carotenoid that could be an additional treatment option. We aimed to measure the effectiveness of administering astaxanthin as an additional therapy to improve the impact of pain and discomfort experienced daily by diabetes mellitus patients with painful diabetic neuropathy. We conducted a randomized experimental study with an open label design of 36 patients who had been diagnosed with …
Heavy Metal Contamination And Potential Ecological Risks In Surface Sediments Along Dar Es Salaam Harbour Channel,
2021
Department of Zoology and Wildlife Conservation, University of Dar es Salaam, Tanzania
Heavy Metal Contamination And Potential Ecological Risks In Surface Sediments Along Dar Es Salaam Harbour Channel, Benard Mwakisunga, Harishchandra B. Pratap, John F. Machiwa, Flora Stephano
Tanzania Journal of Science
Investigation of heavy metal contamination along Dar es Salaam harbour channel was carried out in order to determine their current concentrations, sources and potential ecological risks to benthic biota. Sampling was carried out from January to May 2019 and samples analysed using ICP-OES and C-H-N-S analyser. Analysis of heavy metal relationships showed that, Cu, Zn, Cr, As, Ni, Co, Fe, Cd had strong relationships with each other, while Pb only related to Zn. Principal component analysis partitioned metals into two groups; PC1: Ni, Cr, Fe, Co, As, Cu, Cd, and Zn explaining 73.6% variance and PC2: Pb, Zn, and Cd …
“Data Architecture For Pharmaceutical Product Development At Alkermes”,
2021
Alkermes, Ireland
“Data Architecture For Pharmaceutical Product Development At Alkermes”, Peter Couture, Damon Warnock, Marcus O'Mahony
Level 3
This paper describes how Alkermes built a data architecture for efficient management of non-GXP and R&D data. Using a ‘Create’, ‘Store’, Describe’ ‘Access’ stepwise model, the ingredients to making the journey a success are described.
Acknowledgment Of Reviewers,
2021
Taiwan Food and Drug Administration
Molecular Cartography Of Residue Pesticides On Grape Surface In 3d By Ambient Ionization Tandem Mass Spectrometry,
2021
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
Molecular Cartography Of Residue Pesticides On Grape Surface In 3d By Ambient Ionization Tandem Mass Spectrometry, Hung Su, Tiao-Lai Huang, Cheng-Ting Chi, Yi-Tzu Cho, Chi-Wei Lee, Jingyueh Jeng, Jentaie Shiea
Journal of Food and Drug Analysis
Thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was used to characterize the residual pesticides that were collected from the surface of a grape with metallic sampling probes. Fungicides, insecticides, and miticides were detected, where results were validated by simple solvent extraction followed by gas chromatography tandem mass spectrometry and liquid chromatography tandem mass spectrometry analyses. To explore the distribution of pesticide residues on grape surfaces, 149 locations of a grape surface were collected and followed by TD-ESI/MS/MS analysis. The molecular cartography was then generated from analysis of residual pesticides on the grape surface in 3D.
Variations In Bovine Milk Oligosaccharides After Calving Using P-Aminobenzoic Ethyl Ester Closed-Ring Labeling And Negative Ion Electrospray Lc/Ms/Ms,
2021
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan
Variations In Bovine Milk Oligosaccharides After Calving Using P-Aminobenzoic Ethyl Ester Closed-Ring Labeling And Negative Ion Electrospray Lc/Ms/Ms, Han-Ju Chien, Yi-Chun Ma, Yi-Feng Zheng, Cheng-Yu Kuo, Wei-Chen Wang, Guor-Rong Her, Chien-Chen Lai
Journal of Food and Drug Analysis
A strategy was proposed to analyze bovine milk oligosaccharides using p-aminobenzoic ethyl ester (ABEE) closed-ring labeling and C18 capillary liquid chromatography negative ion electrospray tandem mass spectrometry. Linkage specific fragment ions were used to identify oligosaccharide isomers. By constructing the mass chromatograms using linkage specific fragment ions, isomers were differentiated based on m/z values as well as temporal separation provided by liquid chromatography. In addition to disialyllactose and the single isomer lacto-N-neohexaose, four pairs of linkage isomers including 3’/6’-sialyllactose (3’/6’-SL), 3’/6’-sialyllactosamine (3’/6’-SLN), 3’/6’-sialylgalactosyl-lactose (3’/6’-SGL), and lacto-N-tetraose/lacto-N-neotetraose (LNT/LNnT) in bovine milk were investigated. Variations of bovine milk oligosaccharides in a …
