Pharmacology Commons^™

New Insights On The Differential Interaction Of Sulfiredoxin With Members Of The Peroxiredoxin Family Revealed By Protein-Protein Docking And Experimental Studies, Murli Mishra, Hong Jiang, Qiou Wei

Markey Cancer Center Faculty Publications

Sulfiredoxin (Srx) is the enzyme that restores the peroxidase activity of peroxiredoxins (Prxs) through catalyzing the reduction of hyperoxidized Prxs back to their active forms. This process involves protein-protein interaction in an enzyme-substrate binding manner. The integrity of the Srx-Prx axis contributes to the pathogenesis of various oxidative stress related human disorders including cancer, inflammation, cardiovascular and neurological diseases. The purpose of this study is to understand the structural and molecular biology of the Srx-Prx interaction, which may be of significance for prediction of target site for the novel drug-discovery. Homology modeling and protein-protein docking approaches were applied to examine …

Jinhua Qinggan Granule Uhplc-Q-Extractive-Orbitrap-Ms Assay: Putative Identification Of 45 Potential Anti-Covid-19 Constituents, Confidential Addition, And Pharmacopoeia Quality-Markers Recommendation, Jingyuan Zeng, Xican Li, Rongxin Cai, Chunhou Li, Shaoman Chen

Journal of Food and Drug Analysis

The study combined UHPLC-Q-Orbitrap-MS analysis with authentic standards, to create a novel strategy for isomers recognition and putative identification. Through the strategy, anti-Covid-19 Jinhua Qinggan Granule was found to comprise 28 isomers and 45 potential anti-Covid-19 constituents. The detection of three constituents (Danshensu, cryptotanshin, and tanshinone IIA) suggests Danshen as confidential additive. Based on this, 6 constituents are recommended as quality-marker candidates, including chlorogenic acid, acteoside, peimisine, baicalein, licoricesaponin H2, and tanshinone IIA. Obviously, the study can not only help the public to really understand the Granule's formula and chemistry, but also facilitate its Pharmacopoeia collection in future.

Autophagy-Urea Cycle Pathway Is Essential For The Statin-Mediated Nitric Oxide Bioavailability In Endothelial Cells, Wen-Hua Chen, Bei-Chia Guo, Chia-Hui Chen, Man-Chen Hsu, Chih-Hsien Wang, Tzong-Shyuan Lee

Journal of Food and Drug Analysis

Statins induce nitric oxide (NO) bioavailability by activating endothelial nitric oxide synthase via kinase- and calciumdependent pathways in endothelial cells (ECs). However, their effect on the metabolism of L-arginine, the precursor for NO biosynthesis, and regulatory mechanism have not yet been investigated. In this study, we investigated the role of the autophagy–urea cycle-L-arginine pathway in simvastatin-mediated NO bioavailability in ECs. Griess's assay was used to determine the NO bioavailability. Protein expression was assessed using Western blot analysis. Further, immunocytochemistry was performed to observe autophagosome formation, while conventional assay kits were used to quantify the levels of different intermediate substrates of …

Identification For Metabolism Profiles And Pharmacokinetic Studies Of Tradition Chinese Prescription Ji-Ming-San And Its Major Metabolites In Rats By Uhplc-Q-Tof-Ms/Ms And Uhplc-Ms/Ms, Cheng-Yang Hsieh, Ching-Chiung Wang, Lemmuel L. Tayo, Po-Wei Tsai, Chia-Jung Lee

Journal of Food and Drug Analysis

Ji-Ming-Shan (JMS) is a traditional prescription use for patients with rheumatism, tendons swelling, athlete's foot, diuresis and even gout. This study developed a rapid and sensitive method for the analysis of JMS chemical components in the Traditional Chinese medicine (TCM) prescription and in the serum samples of rats which were administered with the herbal extract. Two mass spectrometric approaches were used namely Ultra-performance liquid chromatographyquadrupole time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) method for the major metabolites of the JMS extract while Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was employed for the detection of the JMS metabolites in the sera of rats. It was …

Cordycepin Inhibits Erk Pathway To Suppress Fgf9-Induced Tumorigenesis With Ma-10 Mouse Leydig Tumor Cells, Li-Ching Chen, Chin-Ying Chen, Yi-Ping Lee, Bu-Miin Huang

Journal of Food and Drug Analysis

Fibroblast growth factor 9 (FGF9) is a member of FGF family, and abnormal expression of FGF9 can promote tumorigenesis. Cordycepin, a major bioactive component in fungus Cordyceps sinensis, could suppress various tumors. We have shown that cordycepin could inhibit FGF9-induced testicular tumor growth in vitro and in vivo with MA-10 mouse Leydig tumor cells. In the present study, the mechanisms related to apoptosis and autophagy were determined. Results show that cordycepin significantly suppressed cell viability and colony formation with correlatedly morphological change related to cell death in FGF9-treated MA-10 cells. Flow cytometry and western blotting results further demonstrate that …

An Integrative Approach For Compressive Quality Control Of Respireaidtm, A Traditional Chinese Medicine Formula Against Sars-Cov-2, Kun-Teng Wang, Chia-Jung Lee, Ming-Chung Lee, Chao-Yu Chen, Yun-Chen Tsai, Wu-Chang Chuang

Journal of Food and Drug Analysis

RespireAid™ (NRICM101) is an effective anti-SARS-CoV-2 traditional Chinese medicine formula and has been licensed as a drug or dietary supplement in Taiwan, Luxembourg, Australia, Singapore, Cambodia, Philippines, and Canada. In this study, we provided integrated quality control strategy to analyze the ingredient of RespireAid™. In addition, the lot-to-lot efficacy stabilities were also evaluated. We found that RespireAid™ comprised of monosaccharides and disaccharides (34.0%), maltodextrin (23.5%), inorganic elements and ash (12.2%), oligosaccharides and polysaccharides (11.4%), principal components (4.4%), moisture (4.0%), amino acids (3.5%), b-Cyclodextrin (0.25%), menthol (0.25%), and nucleotides (0.14%), while the remainder was unidentified (6.36%). This is the first time …

Attenuation Of Diabetes-Mediated Muscle Atrophy In Rats By Fish Oil Enriched Omega-3 Polyunsaturated Fatty Acids Supplementation, Shing-Hwa Liu, Wei-Hsuan Lin, Huei-Ping Tzeng, Meng-Tsan Chiang

Journal of Food and Drug Analysis

Diabetes is associated with an increased risk of muscle wasting/atrophy, which adversely affects quality of life. We hypothesized that long term supplementation of fish oil may have protective effects against sarcopenia or muscle atrophy in streptozotocin (STZ) and high-fat (HF) diet-induced diabetic rat model. Wistar rats at age of 7 weeks were injected with saline or STZ to induce hyperglycemia. After one week, they were fed on a normal control diet or HF diet with/without supplementation of fish oil for 18 weeks. Feeding diabetic rats with a fish oil-enriched diet alleviated body weight loss and the impaired glucose tolerance using …

Ssr-Based Molecular Diagnosis For Taiwan Tea Cultivars And Its Application In Identifying Cultivar Composition Of The Processed Tea, Chih-Yi Hu, Hsien-Tung Tsai, Chui-Feng Chiu, Tsung-Chen Su, Nguyen Hoang Khoi Le, Shu-Dan Yeh

Journal of Food and Drug Analysis

Taiwan specialty teas are produced with distinct manufacturing processes from specific cultivars of tea plants in Camellia. Due to the widespread transplantation of Taiwan tea cultivars and active international trading of tea materials, an accurate and reliable method to identify tea cultivars at the border is vital to protect the image of premium Taiwan specialty teas. In this study, we introduced the Taiwan Tea Variety Identification (TTVID) kit, a capillary electrophoresis-based multiplex PCR assay consisting of 12 simple sequence repeat (SSR) markers. A database composing these 12 SSR loci genotypes in 144 cultivars was established for marker assessment and …

Determination And Evaluation Of Epa And Dha Ethyl Esters In Fish Oils Using The Tmah Transesterification Method, Pai-Wen Wu, Ching-Hsuan Tsai, Ching-Yu Hsu, Shu-Han Chang, Ya-Min Kao, Su-Hsiang Tseng, Der-Yuan Wang

Journal of Food and Drug Analysis

A simple and dependable technique, known as THAM method, has been developed to detect and measure ethyl eicosapentaenoate (EE-EPA) and ethyl docosahexaenoate (EE-DHA) in encapsulated fish oils. This technique involves using tetramethylammonium hydroxide (TMAH) as a catalyst, followed by analysis using gas chromatography equipped with a flame ionization detector. Recoveries of EE-EPA and EE-DHA spiked between 5 mg/g and 20 mg/g were found to be between 90.8% and 95.2%, with coefficients of variation ranging from 0.2% to 2.5%, demonstrating the accuracy and precision of the technique. Additionally, its limitation of quantitation of EE-EPA and EE-DHA in fish oil samples was …

Cannabis Sativa L.: A Comprehensive Review On Legislation, Decriminalization, Phytochemistry, Antimicrobial Activity, And Safety, Mohammed Dalli, Salah-Eddine Azizi, Ali Azghar, Abderrazak Saddari, Elmostapha Benaissa, Yassine Ben Lahlou, Mostafa Elouennass, Adil Maleb

Journal of Food and Drug Analysis

Throughout history, medicinal and aromatic plants have been used extensively to cure a variety of ailments. This article provides a comprehensive overview of Cannabis sativa, specifically focusing on its legislative status, decriminalization, phytochemistry, antimicrobial activity, and safety. The study begins by briefly outlining the plant's history, including its cultivation, harvesting, and storage methods. The review analyzes extensively the antimicrobial properties of Cannabis sativaand its derivatives, specifically examining their reported antiviral, antibacterial, antifungal, and antiparasitic capabilities, which have been documented in databases such as Scopus, ScienceDirect, PubMed, and Web of Science. The paper also discusses trends in studies about the …

How To Deal With Frenemy Nrf2: Targeting Nrf2 For Chemoprevention And Cancer Therapy, Ya-Chu Tang, Yung-Jen Chuang, Hsin-Huei Chang, Shin-Hun Juang, Gow-Chin Yen, Jang-Yang Chang, Ching-Chuan Kuo

Journal of Food and Drug Analysis

Induction of antioxidant proteins and phase 2 detoxifying enzymes that neutralize reactive electrophiles are important mechanisms for protection against carcinogenesis. Normal cells provide multifaceted pathways to tightly control NF-E2- related factor 2 (NRF2)-mediated gene expression in response to an assault by a range of endogenous and exogenous oncogenic molecules. Transient activation of NRF2 by its activators is able to induce ARE-mediated cytoprotective proteins which are essential for protection against various toxic and oxidative damages, and NRF2 activators thereby have efficacy in cancer chemoprevention. Because NRF2 has a cytoprotective function, it can protect normal cells from carcinogens like an angel, but …

Effects Of Green Tea–Derived Natural Products On Resistance Exercise Training In Sarcopenia: A Retrospective Narrative Mini-Review, Hung-Wen Liu, Sue-Joan Chang

Journal of Food and Drug Analysis

Skeletal muscle function deficits result in metabolic disease development and physical dysfunction in older adults. Sarcopenia is characterized by a decrease in muscle mass and strength with advancing age, and it increases the risks of mobility impairments, disease development, and mortality. Lifestyle interventions involving a combination of diet and exercise to prevent and attenuate sarcopenia warrant substantial research attention. Resistance exercise training under supervision is a safe and the most effective approach to reducing age-related muscle loss and improving multiple aspects of overall health in the older population. The beneficial effects of resistance exercise training on skeletal muscle mass may …

Development Of Telodendrimer Nanocarriers For Daptomycin Delivery, Hao Yue

Theses - ALL

The immune system plays a crucial role in fighting pathogens, but infections can occur if the immune system is unable to overcome them, leading to severe damage and death. Antibiotics and drug delivery techniques have helped save countless lives and improve the quality of life for patients. Daptomycin (Dap) is a powerful antibiotic used to treat gram-positive bacterial infections. However, its hydrophobic nature limits its effectiveness, and it requires the presence of calcium ions (Ca2+) for activation. In this study, a series of telodendrimer (TD) nanoparticle delivery systems were synthesized to improve daptomycin delivery. The TD nanoparticle systems showed effective …

Development Of Telodendrimer Nanocarriers For Daptomycin Delivery, Hao Yue

Theses - ALL

The immune system plays a crucial role in fighting pathogens, but infections can occur if the immune system is unable to overcome them, leading to severe damage and death. Antibiotics and drug delivery techniques have helped save countless lives and improve the quality of life for patients. Daptomycin (Dap) is a powerful antibiotic used to treat gram-positive bacterial infections. However, its hydrophobic nature limits its effectiveness, and it requires the presence of calcium ions (Ca2+) for activation. In this study, a series of telodendrimer (TD) nanoparticle delivery systems were synthesized to improve daptomycin delivery. The TD nanoparticle systems showed effective …

Pharmacological And Pharmacokinetic Studies Of A KCa2.2 Positive Allosteric Modulator, Mohammad Asikur Rahman

Pharmaceutical Sciences (PhD) Dissertations

Small-conductance Ca²⁺-activated potassium channels (K_Ca2.x) family is widely expressed in neurons, the heart, and endothelial cells. K_Ca2.x channels are named small conductance Ca²⁺-activated potassium channels due to their comparatively low single-channel conductance and are activated solely by rises in intracellular Ca²⁺. The family has three subtypes: K_Ca2.1, K_Ca2.2, and K_Ca2.3, encoded by KCNN1, KCNN2, and KCNN3 genes, respectively. K_Ca2.x channels regulate neuronal excitability and responsiveness to synaptic input patterns. Small-conductance Ca²⁺-activated potassium channels subtype 2 (K_Ca2.2, …

Altering United States Drug Policy And Shifting The Focus Of Drug Use From Conviction Toward Drug Addiction Treatment, Natalie Marra

Honors Projects

While these substances were not always controlled by law, drug use has been as issue throughout time going back thousands of years and continues to be a concern in the twenty-first century. Possession, distribution, and manufacturing of controlled substances outside of what is considered legal can lead to fines and or jail time and can also increases the severity of other crimes committed either due to or under the influence of drugs. As of Saturday June 10th, 2023, 44.5% of Federal inmates are incarcerated for drug offenses, including possession, distribution, and manufacturing1. Drug offenses make up the largest percentage of …

Self-Administered Intranasal Etripamil Using A Symptom-Prompted, Repeat-Dose Regimen For Atrioventricular-Nodal-Dependent Supraventricular Tachycardia (Rapid): A Multicentre, Randomised Trial, Bruce S Stambler, A John Camm, Marco Alings, Paul Dorian, Hein Heidbuchel, Jaco Houtgraaf, Peter R. Kowey, Jose L Merino, Blandine Mondésert, Jonathan P Piccini, Sean D Pokorney, Philip T Sager, Atul Verma, J Marcus Wharton, David B Bharucha, Francis Plat, Silvia Shardonofsky, Michael Chen, James E Ip

Department of Medicine Faculty Papers

BACKGROUND: Etripamil is a fast-acting, intranasally administered calcium-channel blocker in development for on-demand therapy outside a health-care setting for paroxysmal supraventricular tachycardia. We aimed to evaluate the efficacy and safety of etripamil 70 mg nasal spray using a symptom-prompted, repeat-dose regimen for acute conversion of atrioventricular-nodal-dependent paroxysmal supraventricular tachycardia to sinus rhythm within 30 min.

METHODS: RAPID was a multicentre, randomised, placebo-controlled, event-driven trial, conducted at 160 sites in North America and Europe as part 2 of the NODE-301 study. Eligible patients were aged at least 18 years and had a history of paroxysmal supraventricular tachycardia with sustained, symptomatic episodes …

Investigation Of Antidiabetic Activities Of Cu(Ii) Complex Of Anacardium Occidentale Leaves Crude Extract, Mary Adelaide Oladipo, Folasade Omobolanle Ajao, Adewusi John Adepoju, Kayode Taiwo Ishola, Deborah Omowumi Afolabi

Tanzania Journal of Science

Many synthetic chemical drugs have been widely used for the treatment of diabetes. However, many of these drugs are not locally available, are less effective, and are unaffordable for many diabetic patients in developing and underdeveloped countries. Therefore, in order to search for a locally available, effective, and cost-effective antidiabetic agent, this study synthesized the Cu(II) complex of crude leaf extract of Anacardium occidentale and investigated its antidiabetic activity in alloxan induced albino rats. The leaf crude extract and its metal complex were characterized using atomic absorption spectroscopy (AAS) and infrared (IR) spectroscopy. Experimental diabetic animals were induced by a …

Ameliorative Effects Of Vitamins C And E On Haematotoxicity And Spleen Histopathology Induced By Dichlorvos Insecticide In Female Wistar Rats, Halima Mfaume, Harishchandra Pratap, Claudius Luziga, Felister Urasa

Tanzania Journal of Science

This research aimed to assess the toxicological effects of dichlorvos insecticide (2,2-dichlorovinyl dimethyl phosphate or DDVP) on haematological indices of female Wistar rats and to investigate the antioxidant properties of vitamins C and E in ameliorating haematotoxicity. The rats were divided into 6 groups; control-water, control-oil, dichlorvos group, dichlorvos + vitamin C group, dichlorvos + vitamin E group and dichlorvos + vitamin C + E group. Dichlorvos and vitamins were orally administered to rats with an interval of half an hour between treatments. The control-oil group was given 2 ml corn oil and the control-water group was given water adlib. …

Using Lanthanide Ions As Magnetic And Sensing Probes For The Detection Of Tetracycline From Complex Samples, Yan-Cen Zhan, Chin-Poa Chiu, Yu-Chie Chen

Journal of Food and Drug Analysis

Tetracycline (TC) is a broad-spectrum antibiotic and has been added to animal feeds to grow livestock under healthy conditions, making it important to have effective methods for rapidly detecting TC in complex samples. In this study, a novel method that uses lanthanide ions (i.e. Eu³⁺ and Gd³⁺) as magnetic and sensing probes for the detection of TC from aqueous samples is explored. When dissolving Gd³⁺ in tris(hydroxymethyl)aminomethane (Tris) buffer at pH 9, magnetic Gd³⁺-Tris conjugates can be readily generated. The magnetic Gd³⁺-Tris conjugates possess trapping capacity toward TC from sample solutions via …