Food Science Commons^™

Conventional Solid-State Fermentation Impacts The White Lupin Proteome Reducing The Abundance Of Allergenic Peptides, Arineh Tahmasian, Roger Drew, James A, Broadbent, Angéla Juhász, Mitchell Nye-Wood, Michelle L. Colgrave

Research outputs 2022 to 2026

The demand for high-quality and sustainable protein sources is on the rise. Lupin is an emerging plant-based source of protein with health-enhancing properties; however, the allergenic potential of lupins limits their widespread adoption in food products. A combination of discovery and targeted quantitative proteome measurements was used to investigate the impact of solid-state fermentation induced by Rhizopus oligosporus on the proteome composition and allergenic protein abundances of white lupin seed. In total, 1,241 proteins were uniquely identified in the fermented sample. Moreover, the effectiveness of the solid-state fermentation in reducing the abundance of the tryptic peptides derived from white lupin …

A Novel Machine-Learning Framework Based On A Hierarchy Of Dispute Models For The Identification Of Fish Species Using Multi-Mode Spectroscopy, Mitchell Sueker, Amirreza Daghighi, Alireza Akhbardeh, Nicholas Mackinnon, Gregory Bearman, Insuck Baek, Chansong Hwang, Jianwei Qin, Amanda M. Tabb, Jiahleen Roungchun, Rosalee S. Hellberg, Fartash Vasefi, Moon Kim, Kouhyar Tavakolian, Hossein Kashini Zadeh

Food Science Faculty Articles and Research

Seafood mislabeling rates of approximately 20% have been reported globally. Traditional methods for fish species identification, such as DNA analysis and polymerase chain reaction (PCR), are expensive and time-consuming, and require skilled technicians and specialized equipment. The combination of spectroscopy and machine learning presents a promising approach to overcome these challenges. In our study, we took a comprehensive approach by considering a total of 43 different fish species and employing three modes of spectroscopy: fluorescence (Fluor), and reflectance in the visible near-infrared (VNIR) and short-wave near-infrared (SWIR). To achieve higher accuracies, we developed a novel machine-learning framework, where groups of …

Characterization Of The Probiotic Properties Of Yeast Strains Isolated From Different Food Sources And Unraveling The Potential Nutritional Benefits Of Fermented Date Palm Pomace With Selected Probiotic Candidates: Untargeted Metabolomics And Carbohydrate Metabolites Of In Vitro Digested Fraction, Nadia Al Kalbani

Thesis/ Dissertation Defenses

Utilizing fermentation with probiotics offers a promising opportunity to leverage agricultural by-products, such as date palm pomace (DPP), in order to drive forward sustainable food production endeavors. This dissertation aimed to (1) isolate yeast from fermented food products as well as to characterize their probiotic properties; (2) investigate the physiochemical properties of fermented DPP by selected yeast isolates; (3) evaluate the health benefits of non-fermented and fermented DPP samples both pre and post-digestion (bioaccessible portions); and (4) analyze the untargeted metabolites and carbohydrates metabolites in the bioaccessible portions. The yeast isolates showed remarkable survivability in acidic environment while bile salt …

Value-Added Green Processing Of Tomato Waste To Obtain A Stable Free-Flowing Powder Lycopene Formulation Using Supercritical Fluid Technology, Purlen Sezer Okur, Ozan Ciftci

Department of Food Science and Technology: Faculty Publications

The tomato processing industry generates large amounts of waste, which creates disposal problems and negatively impacts the environment. Tomato waste contains lycopene, high-value health- and wellness-promoting bioactive, and it is a potential source of natural lycopene for food and nutraceutical applications. However, isolation of lycopene from its source requires toxic solvents, and lycopene is unstable during storage. The objective of this study was to extract lycopene from tomato waste and to convert it to a shelf-stable and easy-to-use free-flowing powder form using green extraction and particle formation processes based on supercritical carbon dioxide (SC-CO2) technology. Lycopene was extracted using SC-CO2 …

In Situ Tuning And Investigating The Growth Process Of Size Controllable Gold 2 Nanoparticles And Statistical Size Prediction Analysis, Vinayak Sharma

Articles

A critical understanding of the formation and growth process of gold nanoparticles (GNPs) is crucial, in order to synthesize monodisperse gold nanocrystals of controllable size and shape in a predictable way. GNPs of different shapes and sizes can be produced by altering different physicochemical reaction conditions, such as pH, temperature, and the ratio of reactants. The significant variations in the morphological features of GNPs can be monitored in situ in a time-dependent manner by using UV–visible spectroscopy. In this study, we have synthesized seedless GNPs by reduction of HAuCl4 by using various molar ratios of HEPES and Na2HPO4. The shape …

Valorisation Of Underutilised Dairy Waste Residues: Production Of Lactic Acid Through Microbial Fermentation, Chatan Rai Surana

ORBioM (Open Research BioSciences Meeting)

Annually, approximately 190 million tonnes of liquid waste or co-products is generated by the dairy sector across the globe. These waste streams are nutrient-rich and currently underutilised which gives scope to transform them through microbial fermentation to produce economically valuable products and reduce their negative environmental impact. Such an approach can contribute to the circular bioeconomy by making food production systems more sustainable.

In this study, whey from acid casein hydrolysis (acid whey) and salty whey from Cheddar cheese manufacture were evaluated as feedstocks for production of lactic acid. A total of 466 lactic acid bacteria strains were screened on …

Growth Of Food-Borne Pathogens Listeria And Salmonella And Spore-Forming Paenibacillus And Bacillus In Commercial Plant-Based Milk Alternatives, Klaudia Bartula

ORBioM (Open Research BioSciences Meeting)

An increase in vegan diet preference, lactose intolerance, calorie concern and environmental awareness has led to a rise in the popularity of plant-based alternatives to bovine milk. However, there are still gaps in understanding how known bacterial food contaminants behave in plant-based beverages. The present study is the first to compare the growth of food-pathogens Listeria monocytogenes and Salmonella enterica, food spoilage Bacillus subtilis and an industrial milk product isolate, spore-forming Paenibacillus in commercially available ultrahigh temperature processed bovine milk and plant-based milk alternatives (coconut, almond, cashew). Beverage samples were inoculated with a strain cocktail or individual strains of either …

Genomic Diversity And Carbohydrate Utilisation In Human-Associated Bifidobacterial Isolates, Ortensia Catalano Gonzaga

ORBioM (Open Research BioSciences Meeting)

Bifidobacteria are beneficial commensals of the human gastrointestinal tract and their presence in the gut has been associated with positive health effects on the host. They account for a vast proportion of the infant gut microbiota, when the infant is fed on a milk-based diet, with their number progressively decreasing in adult and elderly. The gut microbiota and associated metabolic activities significantly impact on human health by promoting appropriate development of the infant immune system and contributing to the maintenance of the intestinal homeostasis. In recent years it has become clear that microbial colonization of the gut immediately following birth …

Investigating The Effect Of Agronomic Factors On Microbiome Of Horticulture Produce, Maryada Bohra

ORBioM (Open Research BioSciences Meeting)

The ranged microbiome in fruits and vegetables contributes substantially to the health of vegetation which, in effect, benefits human health. These microorganisms undertake an extensive variety of responsibilities including flavour creation, ripening and health preservation via the production of second-generation metabolites. There is limited information however on the influence of agronomic practices such as crop ripening stage at harvest, storage, packing material and how they impact the microbiome of the crop itself. The overall role of the crop microbiome in gut health is also not fully clear.

The Meta-Hort project will examine the effects of premature harvesting along with tray …

Comparison Of Dna Extraction Methods For The Detection Of Canned Tuna Species With Dna Mini-Barcoding, Aubrey J. Emmi, Biola Fatusin, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Tuna is susceptible to species mislabeling due to its high demand, quick rate of production, and wide range of price points. DNA barcoding, a sequencing-based technique, allows for the detection of species mislabeling by targeting a standardized region of DNA. A mitochondrial control region (CR) DNA barcode has been found to be capable of species discrimination for tuna, but it is challenging to recover the entire DNA fragment from canned tuna. While a short fragment of CR, referred to as a “mini-barcode,” has shown some success with canned tuna species identification, more research is needed to improve identification rates. The …

Storage Stability Of Chocolate Can Be Enhanced Using Locust Protein-Based Film Incorporated With E. Purpurea Flower Extract-Based Nanoparticles, Shubam Singh, Hina F. Bhat, Sunil Kumar, Rana Muhammad Aadil, Maneesha S. Mohan, Charalampos Proestos, Zuhaib F. Bhat

Dairy Science and Food Science Faculty Publications: 2022 to Present

The study aimed to develop a locust protein (Loct-Prot)-based film to enhance the lipid oxidative and storage stability of chocolate. The E. purpurea flower extract based-nanoparticles (EFNPs) were developed using ultra- sonication (500 W and 20 kHz for 10 min) following a green method of synthesis. The EFPNs were incorporated at different levels [T0 (0%), T1 (1.0%), T2 (1.5%), and T3 (2.0%)] to impart bioactive properties to the Loct-Prot- based films which were used for packaging of white chocolate during 90 days trial. The addition of EFPNs increased (P < 0.05) the density of the Loct-Prot-based film which in turn decreased (P < 0.05) the transmittance (%) and WVTR (water vapour transmission rate, mg/mt2) with increasing levels of addition. While brightness (L*) showed a decrease, redness (a*) and yellowness (b*) increased with increasing concentration of EFPNs. No significant (P > 0.05) effect was recorded on other physicomechanical parameters of the film. …

The Association Between Total And Types Of Dietary Protein Intake And Prevelence Of Impaired Fasting Glucose And Type 2 Diabetes, Afra Salah Alsammach

Theses

People with impaired fasting glucose (IFG) and type 2 diabetes (T2D) need to modify their dietary composition. This could assist in the management and prevention of the disease. However, the role played by the composition of specific dietary macronutrients on the risk of IFG & T2D needs further research. This study sought to examine the association between total and type of dietary protein (animal and plant) consumption with prevalence of T2D and IFG. Aims: To investigate the association between total intake and types (animal vs plant) of dietary protein intake and prevalence of IFG & T2D in adults. Data …

Extracts Of Tamarillo, Horned Melon, And Raspberry, But Not Extract Of Pear, Inhibit Human Blood Platelet Aggregation: Investigating The Underlying Factors For Their Differential Mechanisms, Agnese Barin, Ranjit Kumar Das, Nasser E. Bastani, Per Ole Iversen, Asim K. Duttaroy

Health & Biomedical Sciences Faculty Publications

Highlights

• Sugar-free extracts of tamarillo, horned melon (kiwano), and raspberry inhibited ADP-induced platelet aggregation in platelet-rich plasma.

• Analysis of untargeted metabolites revealed the presence of platelet inhibitory components such as benzoic acid, caffeic acid, and gallic acid in the sugar-free extracts of tamarillo, raspberry, and kiwano but not in pear extract.

• Extract of pear had no platelet anti-aggregatory effect.

• Tamarillo, horned melon (kiwano), and raspberry reduced the synthesis of TxB2 and the release of platelet factor 4.

Abstract

In addition to their roles in thrombosis, hyperactive platelets contribute to atherosclerosis. Since anti-platelet drugs are not recommended for …

Characterization Of The Probiotic Properties Of Yeast Strains Isolated From Different Food Sources And Unraveling The Potential Nutritional Benefits Of Fermented Date Palm Pomace With Selected Probiotic Candidates: Untargeted Metabolomics And Carbohydrate Metabolites Of In Vitro Digested Fraction, Nadia Al Kalbani

Dissertations

The use of agricultural by-products, including date palm pomace (DPP), presents a promising opportunity for advancing sustainable food production. By fermenting DPP with novel probiotic yeast candidates, the bioactive properties of the by-product can be enhanced, potentially yielding health benefits. This dissertation aimed to (1) isolate yeast from fermented food products as well as to characterize their probiotic properties; (2) investigate the physiochemical properties of fermented DPP by selected yeast isolates; (3) evaluate the health benefits of non-fermented and fermented DPP samples both pre and post-digestion (bioaccessible portions); and (4) analyze the untargeted metabolites and carbohydrates metabolites in the bioaccessible …

Growing Safer Greens: Exploring Food Safety Practices And Challenges In Indoor, Soilless Production Through Thematic Analysis Of Leafy Greens Grower Interviews, Allyson N. Hamilton, Zeynal Topalcengiz, Kristen E. Gibson

Food Science Faculty Publications and Presentations

Indoor, soilless production—often referred to more broadly as controlled environment agriculture (CEA)—is increasingly used for the cultivation of leafy greens. Minimal information is currently available regarding food safety practices during production and distribution of leafy greens grown within indoor, soilless environments in the United States (U.S.). This study aimed to describe production challenges and implementation of good agricultural practices among CEA growers. Data collection methods included semi‐structured interviews (N = 25) and a supplemental online survey completed by growers (N = 12) in the U.S. Out of 18 total responses (i.e., multiple responses allowed per completed survey), survey data indicated …

Aqueous Ozone Efficacy For Inactivation Of Foodborne Pathogens On Vegetables Used In Raw Meat-Based Diets For Companion Animals, Sahaana Chandran, Christopher A. Baker, Allyson N. Hamilton, Gayatri R. Dhulappanavar, Sarah L. Jones, Kristen E. Gibson

Food Science Faculty Publications and Presentations

The present study evaluates the efficacy of a batch wash ozone sanitation system (BWOSS) and spray wash ozone sanitation system (SWOSS) against Listeria monocytogenes (two strains) and Salmonella enterica subsp. enterica (three serovars) inoculated on the surface of carrots, sweet potatoes, and butternut squash, commonly used in raw meat-based diets (RMBDs) marketed for companion animals such as dogs and cats. Produce either remained at room temperature for 2 h or were frozen at −20°C and then tempered overnight at 4°C to mimic the preprocessing steps of a raw pet food processing operation (‘freeze-temper’) prior to ozone treatment. Two ozone concentrations …

An Amplification-Free Crispr-Sers Biosensor For Specific, Sensitive And Rapid Detection Of Salmonella Typhimurium In Poultry, Fei Jia, Bingyan Li, Yawen He, Yafang Shen, Juhong Chen, Xingmin Li, Yanbin Li

Biological and Agricultural Engineering Faculty Publications and Presentations

CRISPR (Clustered regularly interspaced short palindromic repeats) has been a cutting-edge biotechnology in both gene editing and biosensing due to its desirable features, such as high base resolution and set-and-forget operation. However, as a nucleic acid detection method, an DNA/RNA amplification procedure is still inevitable in CRISPR biosensors. In this study, a highly-ordered nanopore array SERS substrate, coated with silver nanoparticles, was fabricated and utilized as a platform for the specific detection of Salmonella Typhimurium (S. Typhimurium) without the time-consuming DNA amplification step. The smart sensing strategy was established by introducing an ssDNA labeled with a Raman active molecule …

Determination Of Rutin, Hyperoside, Quercetin In Xiang Lotus By Hplc, Zhong Feifei, Li Jing, Lei Deqing, Yang Yun, Wu Jianfang, Ouyang Li

Food and Machinery

Objective: To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. Methods: The HPLC method was performed on an Agilent ZORBAX SB-C₁₈ column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. Results: The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32～2.06, 1.36～2.43, 1.41～3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5～200 g/mL ( …

Nanozymatic Activity Of Zr-Based Mofs: Tuning The Coordinatively Unsaturated Metal Sites For Enhancing Hydrolytic Activity Toward Peptide Bonds, Xu Keyu, Xia Liwei, Xing Keyu, Cheng Yunhui, Xu Zhou

Food and Machinery

Objective: To develop efficient and stable artificial proteases and investigate the relationship between coordinatively unsaturated metal sites and hydrolysis activity of peptide bond. Methods: Three zirconium-based metal-organic frameworks （Zr-MOFs） with different coordinatively unsaturated sites （12-connected Zr-MOF, 6-connected Zr-MOF, and 4-connected Zr-MOF） were prepared to modulate protein hydrolase activity. The protein hydrolase activity of three Zr-MOFs was evaluated by the hydrolysis rate of bis-glycopeptide （Gly-Gly） as an indicator. Subsequently, soy protein, surimi protein and casein were hydrolyzed using the best performing protein hydrolase nanoenzymes, and the hydrolysis products were separated using SDS-polyacrylamide gel electrophoresis, and the hydrolysis efficiency of the three …

Effects Of Three Media On The Isolation Of Two Kinds Of Antimicrobial-Resistant Bacteria From Chicken Products, Huang Liujuan, Fan Yanli, Zhong Hai, Jiang Jing, Shao Yi

Food and Machinery

Objective: To evaluate the isolation effects of different media on antimicrobial risistant （ART） bacteria flora from the surface of cold fresh and cooked chicken products. Methods: Based on high-throughput sequencing technology, the flora structure and abundance of tetracycline-resistant （T^r） and sulfamethazole-resistant （S^r） bacteria isolated by brain heart infusion （BHI）, tryptic soy agar （TSA） and plate count agar （PCA） media was comparatively analyzed. Results: Among the 101 genera of ART bacteria from cold fresh chicken samples, 30, 5 and 3 genera of T^r bacteria were isolated only on BHI, TSA and PCA media respectively, while the …