Food Science Commons^™

Biological Strategies And Mathematical Approaches For Limiting Bacterial Contaminants And Chemical Pollutants In Bioethanol Fermentations, Alia Chahed Ep Limayem

Graduate Theses and Dissertations

During the most recent decades increased interest in fuel from biomass in the United States and worldwide has emerged each time petroleum derived-gasoline registered well publicized spikes in price. The willingness of the U.S. government to face the issues of more heavily high-priced foreign oil and climate change has led to more investment on plant-derived sustainable biofuel sources. Biomass derived from corn has become one of the primary feedstocks for bioethanol production for the past several years in the U.S. However, the argument of whether to use food as biofuel has led to a search for alternative non-food sources. Consequently, …

Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming

Graduate Theses and Dissertations

Campylobacter spp. has been identified as one of the leading causative agents of food borne diarrheal illness. Epidemiological evidence has shown that poultry is the main source for human infection. Poultry are asymptomatic carriers of Campylobacter within their gastrointestinal tract, with colonization levels reaching 106-108 cfu/g cecal content. Surveys of domestic poultry flocks have estimated that approximately 90% of flocks are positive for Campylobacter colonization. Risk assessment studies have determined that by reducing levels of Campylobacter colonization during rearing, incidences of human infection will be significantly reduced. Currently there are no consistently effective treatments to eliminate Campylobacter from poultry flocks. …

Role Of Lactic Acid Bacteria As A Bio-Sanitizer To Prevent Attachment Of Listeria Monocytogenes On Deli Slicer Contact Surfaces, And The Influence Of Listeria Innocua On The Attachment Of L. Monocytogenes On Selected Materials Located In The Food-Processing Environment, Jean Baptiste Ndahetuye

Graduate Theses and Dissertations

Listeria monocytogenes an important foodborne pathogen that continues to be a serious problem for the food industry. This pathogen contaminates food primarily during post-harvest in the food-processing environment. Its ecology in the food-processing environment is not well understood but previous research has demonstrated the ability of L. monocytogenes to survive on food contact surfaces after cleaning and disinfection. The current study explored the attachment of three lactic acid bacteria (LAB) strains, Lactobacillus animalis , Lb. amylovorus and Pediococcus acidilactici , their combination in a cocktail, and their influence to prevent the attachment of L. monocytogenes at room temperature by examining …

Sanitation Assessment Of Food Contact Surfaces And Lethality Of Moist Heat And A Disinfectant Against Listeria Strains Inoculated On Deli Slicer Components, Sabelo Muzikayise Masuku

Graduate Theses and Dissertations

The overall objectives of this study were to: evaluate the efficacy of different cleaning cloth types and cloth-disinfectant combinations in reducing food contact surface contamination to acceptable levels; determine the optimum moist heat and moist heat + sanitizer treatments that can significantly reduce the number of Listeria strains on deli slicer components; and investigate if the moist heat treatment used in this study induced the viable-but-non-culturable (VBNC) state in Listeria cells. The efficacy of wiping cloths was measured using ATP-bioluminescence and total plate count methods using four different wiping cloths and silver dihydrogen citrate sanitizer on food contact surfaces. The …

Characterization Of Igg And Ige Binding To Parvalbumin Derived From Commercially Important Fish Species, Poi-Wah Lee

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Parvalbumin is a calcium-binding muscle protein that is present in all vertebrates. Despite being a pan-allergen in fish and frog, fish-specific IgE and antiparvalbumin IgG antibodies displayed varying cross-reactivity among fish species. In this research, the parvalbumin-binding characteristics of several antibodies were investigated, including 3 IgG antibodies against frog, carp, and cod parvalbumins, and human IgE.

By immunoassay and IgG-immunoblotting, 3 antiparvalbumin antibodies revealed inconsistent specificity among 29 raw fish muscle extracts, which may be partially attributed to the decreased levels of fish muscle parvalbumin from anterior to posterior positions. Parvalbumin-binding by antibodies was unaffected in 112 days of frozen-stored …

Extraction And Identification Of Volatile Constituents Of Oplopanax Horridus , Gregory Jones

All Theses

O. horridus is an important medicinal and spiritual plant for native peoples where it natively grows. Secondary volatile metabolites which compose the essential oil were isolated by atmospheric simultaneous distillation-solvent extraction (SDE) and headspace solid phase microextraction (HS-SPME) from the bark and leaves of Oplopanax horridus. The differences between the two extraction methods was investigated for their sensitivity, precision and use in separating volatile compounds from sample matrices.The extracts were analyzed by capillary gas chromatography with mass spectrometric detection. Compounds were identified according to their non-isothermal Kovats indexes, mass spectra, or by comparison with analytical standard substances. The bark essential …

Development Of Markets For Local Food Crop To Enhance Incomes And Improve Food Security For Smallholder Farmers In East Africa, Betty Bugusu

Changing World Conference

No abstract provided.

Response Surface Methodology Approach For The Optimization Of Lab Fermentation Using Vegetable Based Substrate, Amit Jaiswal, Nissreen Abu-Ghannam, Juan Valverde

Conference papers

In the present studies, an attempt was made to optimize the conditions for the lactic acid bacteria (LAB) fermentation using Irish York cabbage (Brassica oleracea var. capitata alba subvar. conica) as a substrate. Prior to fermentation with Lactobacillus plantarum, York cabbage was blanched at 95°C for 12 min to inactivate surface microflora. To achieve an optimal fermentation condition which would result in higher release of phytochemicals and antioxidant capacity in the broth, Box-Behnken design integrating a desirability approach was used. The optimized factors (fermentation time 36h, solid/liquid ratio 0.25 g/ml and agitation rate 100 rpm) were used for …

Effect Of Solvents On The Extractability Of Phenolic Constituents And Their Antioxidant Capacity From Irish Seaweed, Gaurav Rajauria, Amit Jaiswal, Nissreen Abu-Ghannam

Conference papers

Seaweeds are being used for human consumption in the Orient and help them live longer with low levels of hypertension, cancer and other ailments. The study aimed at extraction of principle constituents using a wide range of solvents and their mixtures on the basis of polarity, from Irish brown seaweeds namely Himanthalia elongata, Laminaria saccharina and Laminaria digitata. All the extracts were screened for total phenolic content (TPC) and their potential antioxidant capacity, using 2, 2´-diphenyl-1-picrylhydrazyl (DPPH) radical and ferric reducing antioxidant power (FRAP) assay. Among all the solvents tested, 60% methanolic extract and equi-volume mixture of chloroform, …

Bioactive Potential Of Marine And Terrestrial Vegetables: A Comparative Study, Amit Jaiswal, Gaurav Rajauria, Nissreen Abu-Ghannam

Conference papers

Polyphenols are extensively used in food, beverage, pharmaceuticals and nutraceutical industry for their positive effects on human health. Present study was designed to estimate the differences in polyphenols level and antioxidant capacity between marine and terrestrial vegetables. Considerable variations in the total phenolic content (TPC) and total flavonoid content (TFC) were observed, which ranges from 20.4 to 140.2 mg GAE/g of extracts (dw) for TPC and 15.4 to 50.4 mg QE/g of extracts (dw) for TFC. Furthermore, antioxidant capacity was confirmed by all the extracts. Results showed that marine vegetables had significantly higher polyphenols content and antioxidant property compared to …

Analysis Of Zinc And Copper In Foods, Merlly Zavala

Undergraduate Research Conference

Deficiencies in vitamins and calcium have always been known and a remedy for it has always been around. The deficiency of copper (Cu) and zinc (Zn) are not very popular and some people do not understand how important the two metals are for one’s health. If one tends to encounter such deficiency the goal is to give foods that contain these metals in order to add to the diet. In this study, various foods were analyzed for Cu or Zn concentration levels using absorptions spectrophotometer Shimadzu AA-6800 flame. The samples were crushed, digested with nitric acid, and then analyzed through …

Amphiphilic Polyanhydride Nanoparticles Stabilize Bacillus Anthracis Protective Antigen, L. K. Petersen, Y. Phanse, Amanda E. Ramer-Tait, M. J. Wannemueler, B. Narasimhan

Department of Food Science and Technology: Faculty Publications

Advancements towards an improved vaccine against Bacillus anthracis, the causative agent of anthrax, have focused on formulations composed of the protective antigen (PA) adsorbed to aluminum hydroxide. However, due to the labile nature of PA, antigen stability is a primary concern for vaccine development. Thus, there is a need for a delivery system capable of preserving the immunogenicity of PA through all the steps of vaccine fabrication, storage, and administration. In this work, we demonstrate that biodegradable amphiphilic polyanhydride nanoparticles, which have previously been shown to provide controlled antigen delivery, antigen stability, immune modulation, and protection in a single …

Table Of Contents April 2012 Agricultural Research Magazine

Agricultural Research Magazine

Table of Contents

4 A Bit of Gold and Nanotechnology Bring Viruses to Light

6 Digital Detectives Decipher Ingredients

8 SERS: High-Tech Tactic May Newly Expose Stealthy Salmonella

10 What’s in Your Blood? The Ongoing Hunt for Metabolites

12 Protein Biomarkers Identify Disease-Carrying Aphids

13 A New Approach to Molecular Plant Breeding

14 Cotton Gets Nanotech and Biotech Treatment in New Orleans

16 Barcoding Insects To Control Them

18 Metagenomics Offers Insight Into Poultry Diseases

20 Longstanding Erosion Calculator Enters 21st Century

23 Locations Featured in This Magazine Issue

Forum: Innovations Through Biotechnology, Jack Okamuro, Kay Simmons

Agricultural Research Magazine

"We need to put a premium on creating innovative solutions to address our current and future problems."- U.S. Department of Agriculture Secretary Tom Vilsack on priorities for the 2012 Farm Bill.

This month in Agricultural Research, we highlight Agricultural Research Service projects grown from seeds planted on what were once the distant horizons of biotechnology- metagenomics, genomic selection, metabolomics, and more.

Each project represents a fusion of leading-edge science and technological innovation that is helping to shape ARS's response to the growing food, fiber, and fuel needs of a U.S. and world population forecasted to exceed 430 million and 9 …

Back Matter Agricultural Research Magazine 60(4): April 2012

Agricultural Research Magazine

U.S. Department of Agriculture

Agricultural Research Magazine

5601 Sunnyside Ave.

Beltsville, MD 20705-5129

Barcoding Insects To Control Them, Dennis O’Brien

Agricultural Research Magazine

Mention barcodes and it often brings to mind the sales tags and scanners found in supermarkets and other stores. But Agricultural Research Service scientists are using “DNA barcodes” in their search for ways to control and monitor insects that pose the greatest threats to crops as diverse as wheat, barley, and potatoes.

In DNA barcoding, scientists sequence a designated part of an organism’s genome and produce a barcode from it for a systematic comparison with the sequenced DNA of other closely related species. DNA barcodes are being developed on a wide range of plants and animals as part of a …

Cotton Gets Nanotech And Biotech, Jan Suszkiw, Brian Condon

Agricultural Research Magazine

Scientists at the Agricultural Research Service’s Cotton Chemistry and Utilization Research Unit (CCUR) in New Orleans, Louisiana, have a long history of research successes leading to advances in the use, manufacturing, and quality of cotton fiber.

For example, groundbreaking studies led by chemist Ruth Benerito at the Cotton Chemical Reactions Laboratory (CCUR’s predecessor), starting in the 1950s, gave rise to easy-care, permanent-press clothing and other consumer-friendly improvements that helped cotton better compete with synthetic fibers, like polyester and nylon.

New challenges and consumer demands have since emerged, but the ARS lab’s tradition of excellence and innovation in research continues.

High-Tech Tactic May Newly Expose Stealthy Salmonella, Marcia Wood, Bosoon Park

Agricultural Research Magazine

At laboratories of the future, even the smallest quantity of Salmonella bacteria may be easily detected with a technology known as “SERS,” short for “surface-enhanced Raman scattering.”

Agricultural engineer Bosoon Park, in the Agricultural Research Service’s Quality and Safety Assessment Research Unit in Athens, Georgia, is leading exploratory studies of this analytical technique’s potential for quick, easy, and reliable detection of Salmonella and other foodborne pathogens.

According to the U.S. Centers for Disease Control and Prevention, Salmonella causes more than 1 million cases of illness in this country every year.

Long-Standing Erosion Calculator, Don Comis, Robert Sowers

Agricultural Research Magazine

Seth Dabney is busy tweaking a soon-to-be-unveiled update of the Revised Universal Soil Loss Equation, Version 2 (RUSLE2), which moves the original equation ever further from its origins in the age of slide rules to the era of computing. Dabney is research leader of the Watershed Physical Processes Research Unit, at the Agricultural Research Service’s National Sedimentation Laboratory in Oxford, Mississippi.

RUSLE2 has retained the integrity of the original Universal Soil Loss Equation (USLE)—in greatly expanded form—and integrated an updated database with a computer model that reflects both the latest in computer technology and scientific discoveries about erosion processes. It …

Digital Detectives Deciper Ingredients, Rosalie Marion Bliss, James M. Harnly, Pei Chen

Agricultural Research Magazine

Television shows featuring crime scene investigators have been keeping viewers intrigued for years. But the Agricultural Research Service’s intriguing “food composition investigators” are just as innovative at deciphering truth from fiction relating to ingredients of plant-based foods and dietary supplements. The researchers are at the ARS Food Composition and Methods Development Laboratory, which is headed by research leader James Harnly. The laboratory is part of the Beltsville [Maryland] Human Nutrition Research Center (BHNRC).

They’re using new equipment and a metabolomics approach to discover compounds and to accurately identify ingredients in foods and supplements. They are also looking at chemical composition …