Food Science Commons^™

Isolation And Identification Of Antagonistic Bacteria Aganist Vibrio Parahaemolyticus And Its Characteristics Of Antibacterial Substances, Hao Yanli, Wang Liping, Huang Yuliang, Xue Meicui, Cui Yunyun, Zhao Yong, Li Li, Wang Zhengquan

Food and Machinery

In order to screen a probiotic strain against foodborne pathogens and aquatic pathogens, the main zoonotic pathogenic bacteria of mariculture Vibrio parahaemolyticus ATCC17802 was used as indicator bacterium. The target probiotics was screened using the spot-on-lawn method and rescreened by the agar diffusion method, and the diameter of inhibition zone of antibacterial substances reached (17.16±0.28) mm. Through the combination of morphology and 16S rDNA sequence analysis, strain H19 was identified as Bacillus subtilis (GenBank accession number: MG383451). Antimicrobial spectrum of B. subtilis H19 was tested, meanwhile the physical and chemical properties of antimicrobial substances produced by H19 was studied. The …

Effect Of Different Tipping Glue And Tipping Paper On The Permeability Of Glue, Zhang Jing, Ma Xiaowei, Feng Xin, Qu Guofu, Wang Dongfei, Wang Jianmin

Food and Machinery

In order to evaluate the effect of glue penetration on cigarette filter ventilation rate, and provide the basis for tipping glue and tipping paper selection, using the PVC rectangular pore pressure plate control the area of the original coating area, calculate the penetration area of coloring glue using pixel method, estsblished a method to measure the natural permeability of the tipping glue, and the natural permeability of 2 kinds of tipping glue on 12 kinds of tipping paper was measured and analyzed. The results showed that: ① the natural permeability of 2 kinds of tipping glue on 12 kinds of …

Optimization On Preparation Of Osmanthus Extracts By Enzymes Treatment, Zhang Xuesong, Pei Jianjun, Zhao Linguo, Tang Feng, Fang Xianying

Food and Machinery

To improve the yield and quality of Osmanthus extracts, several techniques were applied including the enzymatic hydrolysis by β-glucosidase and pectinase complex along with petroleum ether extraction and GC-MS analysis. The enzymatic hydrolysis process was optimized usingresponse surface methodology, and a reliable multiple quadratic regression model was established. The results showed that the best levels of the factors were as followed: pH 4.7, time 2.6 h, temperature 46 ℃, ratio of material to liquid 19.81 (mL/g), and the amount of enzyme complex 54.4 IU/g with 48.1% β-glucosidase activity, which was confirmed by experiments with an extraction yield of 3.32%, an …

Effect Of Surrounding Vegetation On Microbial Survival Or Die-Off On Watermelon Surface In An Agriculture Setting, Vijay Singh Chhetri, Kathryn Fontenot, Ronald Strahan, Veerachandra K. Yemmireddy, Katheryn J. Parraga Estrada, Achyut Adhikari

School of Earth, Environmental, & Marine Sciences Faculty Publications

Preharvest contamination of produce with food borne pathogens has been a major food safety issue. In this study, we investigated the effect of surrounding vegetation on the survival of natural and inoculated generic Escherichia coli on watermelon rinds in an agricultural field setting. There was no significant difference (p > .05) on the populations of natural generic E. coli (1–1.46 log Most Probable Number (MPN)/sample) and coliforms (E. coli inoculated on watermelon rind discs was variable with the level of vegetation. A significant reduction in generic E. coli count was observed within 12 hr at all vegetation levels. After 108 …

Food Waste Posing A Serious Threat To Sustainability In The Kingdom Of Saudi Arabia – A Systematic Review, Mirza B. Baig, Khodran H. Al-Zahrani, Felicitas Schneider, Gary S. Straquadine, Marie Mourad

Applied Sciences, Technology and Education Faculty Publications

Worldwide, food waste is one of the prime issues threatening food security and the Kingdom of Saudi Arabia (KSA) is not an exception. With 427 kg of food wasted per capita per year, the country ranks among the top food wasters. Ironically, the Kingdom has limited arable lands and scarce water resources to support mass-scale agriculture and to feed its increasing population, KSA relies heavily on imports and subsidized food to meet needs. Yet, food is wasted at restaurants, caterers, cafeterias and, especially, by households such that food waste is the single-largest component of the landfills. The review article is …

Species Substitution And Country Of Origin Mislabeling Of Catfish Products On The U.S. Commercial Market, Shayna A. Bosko, Denise M. Foley, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Catfish belong to the order Siluriformes and include both the Ictaluridae and Pangasiidae families. However, U.S. labeling laws require only species of the family Ictaluridae to be marketed as catfish. The lower production price of Pangasiidae, combined with changes in regulations over time, have resulted in high potential for species substitution and country of origin mislabeling among catfish products. The objective of this study was to conduct a market survey of catfish products sold at the U.S. retail level to examine species mislabeling and compliance with Country of Origin Labeling (COOL) regulations. A total of 80 catfish samples were collected …

Evaluation Of Microbial Safety And Quality Of Louisiana Strawberries After Flooding, Shifa Shiraz

LSU Master's Theses

Rainfall and flooding have impacted the strawberry industry in Louisiana. Floodwater is an ideal medium for microbiological growth and may cause contamination of soil, agricultural water, and fresh produce with foodborne pathogens. This research aimed to evaluate the microbial safety and quality of strawberries after flooding. Three strains of generic Escherichia coli were used to establish a baseline population of approximately 10⁶ CFU/L (high contamination) and 10² CFU/L (low contamination) in floodwater. Five raised beds were filled with water to simulate a flooding event. Simulated floodwater was mixed with cow manure and spiked with generic E. coli then …

Colonization Of Breastfed Infants By Bifidobacterium Longum Subsp. Infantis Evc001 Reduces Virulence Gene Abundance, Giorgio Casaburi, Steven A. Frese

Department of Food Science and Technology: Faculty Publications

The infant gut microbiome is rapidly colonized by bacteria from the environment after birth, and this gut ecosystem can facilitate expansion of potential pathogens. Human milk shapes the infant gut microbiome and has evolved to foster the growth of specific bacteria. Breastfed infants fed the coevolved infant gut symbiont Bifidobacterium longum subsp. infantis EVC001 had significant modifications to their gut metagenome, including a decreased number of virulence factor genes.

Survival And Inactivation Of Salmonella Enterica And Human Norovirus On Consumer Contact Surfaces, Dorra Simmons

LSU Doctoral Dissertations

Salmonella enterica and human norovirus (HuNoV) are the leading cause of bacterial and viral gastroenteritis worldwide, respectively. One of the most important transmission routes of these pathogens is contaminated surfaces. In this study, we investigated the survival and the disinfection of Salmonella enterica serovar Typhimurium (S. Typhimurium) and HuNoV on selected consumer contact surfaces.

In the case of S. Typhimurium, formica laminate, stainless steel, and plastic cutting board surfaces were inoculated with different microbial loads of cells collected at log (6 h), stationary (24 h), or LTS (14 days) phases. The disinfection efficacy of two disinfectants (sodium hypochlorite or …

A Systematic Literature Review For Evidence Of Aphanizomenon Flos-Aquae Toxigenicity In Recreational Waters And Toxicity Of Dietary Supplements: 2000–2017, Amber Lyon-Colbert, Shelley Su, Curtis Cude

Kinesiology & Public Health Education Faculty Publications

Previous studies of recreational waters and blue-green algae supplements (BGAS) demonstrated co-occurrence of Aphanizomenon flos-aquae (AFA) and cyanotoxins, presenting exposure risk. The authors conducted a systematic literature review using a GRADE PRISMA-p 27-item checklist to assess the evidence for toxigenicity of AFA in both fresh waters and BGAS. Studies have shown AFA can produce significant levels of cylindrospermopsin and saxitoxin in fresh waters. Toxicity studies evaluating AFA-based BGAS found some products carried the mcyE gene and tested positive for microcystins at levels ≤ 1 μg microcystin (MC)-LR equivalents/g dry weight. Further analysis discovered BGAS samples had cyanotoxins levels exceeding tolerable …

Stability Of The Human Faecal Microbiome In A Cohort Of Adult Men, Raaj S. Mehta, David A. Drew, Jason Lloyd-Price, Ayshwarya Subramaian, Paul Lochhead, Amit D. Joshi, Kerry L. Ivey, Hamed Khalili, Gordon T. Brown, Casey Dulong, Mingyang Song, Long H. Nguyen, Himel Mallick, Eric B. Rimm, Jacques Izard, Curtis Huttenhower, Andrew T. Chan, Galeb S. Abu-Ali

Department of Food Science and Technology: Faculty Publications

Characterizing the stability of the gut microbiome is important to exploit it as a therapeutic target and diagnostic biomarker. We metagenomically and metatranscriptomically sequenced the faecal microbiomes of 308 participants in the Health Professionals Follow-Up Study. Participants provided four stool samples—one pair collected 24–72 h apart and a second pair ~6 months later. Within-person taxonomic and functional variation was consistently lower than between-person variation over time. In contrast, metatranscriptomic profiles were comparably variable within and between subjects due to higher within-subject longitudinal variation. Metagenomic instability accounted for ~74% of corresponding metatranscriptomic instability. The rest was probably attributable to sources such …

Potential Mechanisms For Nns-Induced Metabolic Deviances: Satiety Hormone Secretion And Alterations In The Gut Microbiota, Kyle J. Decker

DePaul Discoveries

Rising rates of obesity and type 2 diabetes have prompted the usage and recommendation of nonnutritive sweeteners (NNS) as harmless sugar substitutes in attempts to decrease caloric intake. Contrary to the common belief that NNS remain physiologically inert post-consumption, evidence highlights their ability to alter metabolic processes via interactions in the gastrointestinal tract. An extensive review was conducted on the potential NNS-induced metabolic deviances by way of two non-mutually exclusive mechanisms. One possible mechanism involves their ability (or inability) to induce the secretion of GLP-1, a hormone produced in the gut that promotes satiety and accelerates glucose-dependent insulin secretion by …

Effect Of Ultrasounds Ynergistic Glycosylation On The Sensitization And The Structure Of Egg White Powder, Song Qidong, Tu Zongcai, Wang Hui, Yang Wenhua, Liu Guangxian, Lu Jianwei

Food and Machinery

The commercially available egg white powder was taken as the research object. The effects of ultrasound synergistic glycosylation on protein structure and sensitization were studied by electrophoresis, ultraviolet spectrum, fluorescence spectroscopy and ELISA. The results showed that ultrasound combined with glycosylation could reduce the sensitization of egg white powder significantly, and the reduce of hypersensitivity level was the largest when the ultrasound intensity was around 15 min and 600 W. This change was closely related to its structure, and the egg white protein molecular weight increased significantly after combined treatment. Moreover, the three stages of structure change significantly resulted in …

Expression Of Bacillus Acidopullulyticus Pullulanase In Escherichia Coli And Its Optimization Of Fermentation, Yang Xianghui, Chen Sheng, Wu Jing

Food and Machinery

To increase expression and extracellular secretion of pullulanase of the recombinant strain E.coli BL21(DE3)/pET20b(+)-BapulA at 3 L fermentor level. Expression of recombinant pullulanase in E. coli and further optimization of fermentation at the 3 L fermenter Firstly. The effects were investigaged, including the fermentation temperature, pH, IPTG concentration, induction time and glycine concentration on the expression of recombinant pullulanase. The optimum fermentation conditions were showed as followed: the growth temperature and pH of the pre-induction cells were 30 ℃ and 7.0, respectively. When the cell density reached 50 OD₆₀₀, the fermentation temperature was shifted to 25 ℃, and …

Differences Of The Properties In Three Kinds Of Zanba Powder By Layered Grinding And Its Correlation Analysis, Ma Jie, Zhou Yang, Chen Luyao, Peng Xiyu, Lu Ying, Guo Shuntang

Food and Machinery

With three different varieties of highland barley (Zangqing 320, Zangqing 2000, Goumang) as raw material, investigated the β-glucan content, pasting properties and aroma characteristics of cooked highland barley (Zanba) obtained from layered milling method. The results showed that, from outer to inner layer (Zanba powderⅠ～Ⅵ), the samples were exhibited with increasing β-glucan content, as well as improved initial and final viscosity values for the three highland barleys. Furthermore, the total peak area and the flavor in gas chromatography showed a decreasing tendency, which indicated the flavor characteristics of outer layer Zanba powder were better than that of the inner layer …

Preparation Of Crosslinked Welan Gum And Its Properties, Chen Qianwen, Tong Qunyi

Food and Machinery

The cross-link modification of welan gum was studied by single factor experiment with epoxychloropropane (EPC) as cross-linking agent. The best conditions for the preparation of cross-linked welan gum were EPC added amount was 12 μL/g, the reaction at the solution of pH 8, at 40 ℃ for 4 hours. The results showed that welan gum and crosslinked welan gum both had good acid and alkali resistance, but crosslinked welan gum had better viscoelasticity, salt tolerance than welan gum. After high temperature treatment, crosslinked welan gum got better temperature resistance. Then scanning electron microscopy proved that the cross-linking strengthened the network …

Study On Correlation Between Organizational Structure And Puncturing Texture Of Jujube, Chen Kai, Zhou Tong, Xu Mingqiang, Fu Bing, Ma Xin, Li Huanrong

Food and Machinery

In order to analyze the correlation of the organizational structure and texture properties of fresh jujube fruits in Xinjiang,the three cultivars were studied as follows: Jun jujube, Hui jujube and Hami jujube. The results showed that the charateristics of Hui jujube, such as thickness of cuticle（6.34 μm）, epidermis thickness（36.41 μm）, epidermal cells（118.21 μm²）, near epidermis cavity（9 160.98 μm²）, near epidermal cells（892.71 μm²） and central cells（2 766.68 μm²） were smaller than that of Jun jujube and Hami jujube, there were significant differences in organizational structure except epidermal cells among the cultivars. While, skin …

Yield And Quality Of The Sprouts From Different Safflower Varieties (Lines), Zhu Xiaoqing, Du Weiwei, Wang Qing, Hou Kai, Xiao Renfeng, Wu Wei

Food and Machinery

This paper is aimed at studying the relationship between the safflower seeds with different linoleic acid content and its sprouts quality, to provide reference for the scientific production of safflower sprouts. The yield and quality of the safflower sprouts from 8 varieties (lines) with different linoleic acid content were analyzed and compared. The results showed linoleic acid content of PI305192 in seeds was the highest.The hypocotyl length and edible fresh weight of PI305192 sprouts were the highest, its edible fresh weight was significantly higher than that of other varieties (lines) except for PI544040.The contents of linoleic acid of Chuanhong No. …

Determination Of 6 Fluoroquinolones Residues In Aquatic Products By Solid Phase Extraction And Ultra Performance Lquid Chromatography, He Jiang, Li Xiaoyue, Qiu Yujie, Li Boen, Yang Pinhong

Food and Machinery

Based on solid phase extraction (SPE) and ultra high performance liquid chromatography (UPLC) technique, a method has been developed for the simultaneous detection and analysis of 6 fluoroquinolones residues in aquatic products. The optimized process and parameters of this method were as followed: treated aquatic products were extracted with phosphate buffer, the extracting solution were purified by SPE and concentrated by blowing nitrogen, and the residues were then dissolved in 0.2% formic acid solution for UPLC analysis; Chromatographic column BEH C₁₈ (1.7 μm, 2.1 mm×50 mm) were applied, and citric acid (0.05 mol/L)-ammonium acetate (0.1 mol/L) buffer were used …

Design And Research Of Low Temperaturewear Simulation Testing Machine, Shen Peng, Cao Huiqiong, Qiao Yuli

Food and Machinery

On basis of the simulation of the metal contact behavior under low temperature condition, an experimental device for metal simulation testing under low temperature was designed and fabricated; then its designing method, operation, key technological parameters, and points for attention in usage and components such as rolling testing bench, computer control system, cooling system which are introduced in detail. The testing machine has many advantages as follow: Range of -60～0 ℃ ambient temperature can be simulated steadily, which can be offset automatically and precisely through low temperature solenoid valve; The experimental speed of specimenⅠ/ specimenⅡ can be adjusted independently as …