Food Science Commons^™

Spectral Lights Based Treatment Enhanced Biomass Accumulation And Secondary Metabolites Production In Callus Culture Of Citrus Reticulata, Faiza Anum, Naveed Iqbal Raja, Tahira Sultana, Abeer Kazmi, Amir Ali, Bushra Qayyum, Anza Afzal, Aneela Nijibat, Zia-Ur-Rehman Mashwani, Abeer Kazmi

The Philippine Agricultural Scientist

Citrus reticulata is a common fruit, belongs to the family Rutaceae. It contains terpenes, phenolics, flavonoids, and vitamin C in conjugation with sugars, amino acids, minerals, organic acids and other biologically important compounds. Application of light elicitation strategies in in vitro plant cultures is acquiring more interest for their potential in feasible biomass accumulation and therapeutically important secondary metabolites production. The present study was designed to investigate the effects of different monochromatic lights (white, red, blue, green, and yellow) on the proliferation and secondary metabolites accumulation in callus cultures of Citrus reticulata raised in response to treatment with AgNPs and …

An Evaluation Of The Hargreaves-Samani Method For Estimating Evapotranspiration Under Semi-Arid Conditions, Yusuf Aydin

The Philippine Agricultural Scientist

It is suggested by the Food and Agriculture Organization of the United Nations (FAO) to use the FAO56-Penman-Monteith (FAO56-PM) model as the standard model in reference crop water consumption studies. However, the climate parameters required by the standard model cannot be fully and reliably obtained in under developed or developing countries. Hence, the Hargreaves-Samani (HS) equation is used as an alternative to the FAO56-PM model since it can be used for calculations with less climate data. In this study, ET_o-PM and ET_o-HS values calculated at 8 provinces in Southeastern Turkey using the IAM-ET_o software were evaluated and …

Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol

Dissertations, Theses, and Capstone Projects

The hop plant (Humulus L., Cannabaceae) is a dioecious, perennial, twining vine with a long history of human use. Nowadays, hop plants are generally grown for their inflorescences (“cones”), which are used in brewing for their phytochemical metabolites. Many of these metabolites are involved in plant stress response and communication. Genetics and environment are two major factors that affect plant metabolism. In three separate metabolomics studies, this project examined the effects of both genetic and environmental factors on hop phytochemistry.

In the first study, 23 hop genotypes were grown in two different locations in the Pacific Northwest region of …

Sweetpotato Leaves Inhibit Lipopolysaccharide-Induced Inflammation In Raw 264.7 Macrophages Via Suppression Of Nf-Κb Signaling Pathway, Hyun-Dong Cho, Cindi Brownmiller, Harun Sorker, Shahidul Islam, Sun-Ok Lee

Food Science Faculty Publications and Presentations

Limited information is available regarding the health-promoting activities of sweetpotato leaves (SPL). The present study investigated antioxidant and anti-inflammatory effects, and phenolic contents in 29 SPL cultivars harvested in 2018 and 2019. Extracts showed total phenolic contents 9.4–23.1 mg gallic acid equivalent/g, and DPPH radical scavenging activity indicated 36.6–247.3 mM of Trolox equivalent/g. SPL extracts were identified to contain bioactive components such as, chlorogenic acid (11.7–22.1 μg/mg), 3,4-dicaffeoylquinic acid (16.3–59.9 μg/mg), 3,5-dicaffeoylquinic acid (50.9–72.7 μg/mg), chlorophyll B (6.1–12.3 μg/mg), lutein (1.9–4.9 μg/mg), chlorophyll A (2.7–4.3 μg/mg) and β-carotene (0.1 ≤ μg/mg). RAW 264.7 murine macrophage cells were pretreated with 100–200 …

Dha But Not Epa Induces The Trans-Differentiation Of C2c12 Cells Into White-Like Adipocytes Phenotype, Saeed Jebor Ghnaimawi, Lisa Rebello, Jamie Baum, Yan Huang

Food Science Faculty Publications and Presentations

Muscle derived stem cells (MDSCs) and myoblast play an important role in myotube regeneration when muscle tissue is injured. However, these cells can be induced to differentiate into adipocytes once exposed to PPARγ activator like EPA and DHA that are highly suggested during pregnancy. The objective of this study aims at determining the identity of trans-differentiated cells by exploring the effect of EPA and DHA on C2C12 undergoing differentiation into brown and white adipocytes. DHA but not EPA committed C2C12 cells reprograming into white like adipocyte phenotype. Also, DHA promoted the expression of lipolysis regulating genes but had no effect …

Understanding The Influence Of Zinc On Grain Cadmium Accumulation And Bioaccessibility In Rice, Michael A. Tavarez

Dissertations, Theses, and Capstone Projects

The effect of cadmium and zinc on mineral concentrations in three cultivars of rice

Interactions between the essential mineral zinc (Zn) and the toxic heavy metal cadmium (Cd) play an important role in regulating transport of both minerals to rice grains. Understanding these interactions is crucial for limiting cadmium and increasing zinc transfer to the food chain. Previous studies on the matter have had conflicting results suggesting synergistic and antagonistic relationships between the minerals. The goal of this work was to identify the effect of external cadmium and zinc on the uptake and translocation of both minerals from roots to …

Soft Camel Milk Cheese Effects Of Coagulants And Processing Conditions, Mustapha Mbye

Dissertations

In recent years, camel milk (CM) has been acknowledged for several health benefits, including anti-diabetic, hypoallergic, and other effects. Accordingly, CM may provide a complementary or a healthier alternative to bovine milk (BM). This has led to increased interest in processing CM to products like cheese, yogurt, and powders to extend its shelf-life. However, CM is difficult to coagulate into hard gels, affecting its cheese quality and consumer preference. The current research aimed to investigate the impact of different coagulants (chymosin, Withania coagulans, citric and acetic acid) on CM cheese quality and sensory attributes compared to BM cheese. It …

Biocontrol Of Foodborne Pathogens Using Bacteriophages, Dzhuliya Ignatova, Erion Hogan, Simone Dakare, Jean Lu

Symposium of Student Scholars

Biocontrol of Foodborne Pathogens Using Bacteriophages

Dzhuliya Ignatova, Erion Hogan, Simone Dakare, and Jean Lu

Department of Molecular and Cellular Biology

Abstract

Salmonella and Shigella are two important groups of foodborne bacterial pathogens. Salmonella cause an illness called salmonellosis while Shigella cause shigellosis (bacillary dysentery). The most common symptoms of these illnesses are abdominal cramps, diarrhea, and fever. Shigella can also cause bloody diarrhea. It was estimated that each year Salmonella cause 93.8 million cases of gastroenteritis and 155,000 deaths globally. Shigella causes 164.7 million cases and 1.1 million deaths throughout the world yearly. People get these illnesses mainly by …

Bacteriophages Infecting Enterobacter Cloacae To Reduce Bloater Damage In Fermented Cucumbers

Symposium of Student Scholars

Fermented cucumbers are one of the most important fermented vegetables consumed worldwide. During cucumber fermentations, certain undesirable changes may occur. One of such changes is known as bloater defect (hollow cavities in fermented cucumbers), which is primarily caused by gas-producing bacteria including Enterobacter cloacae. Bloater defect lowers product quality and leads to significant economic loss to the pickle industry, and effective preventative methods are needed. Bacteriophages (phages) are highly host-specific bacterial killers. Use of phages to control unwanted bacteria in foods is a promising approach because phages do not change food properties. This research was to isolate, characterize, and …

In Silico Identification Of A Streptococcus Phage From An Unpasteurized Dairy Product

Symposium of Student Scholars

This study aimed to identify novel phages from a West African dairy product, nunu, to better understand the uncharacterized virosphere of a food microbiome. Nunu is a yogurt-like product from spontaneous fermentation of unpasteurized cow milk without a standardized starter culture. Phages and their bacterial hosts make up a microbiome, where their collective metagenome can be studied in silico, or computationally, after next generation sequencing (NGS). The targeted dataset for our study can be found in the Sequence Read Archives under the accession number of ERX2041567. This study utilized several bioinformatics software tools with customized settings: Kraken2, MetaVelvet, Edena, NCBI-BLAST, …

In Silico Isolation Of A Novel Phage For Food Safety Applications Against Pathogenic E. Coli, Daisy Mcgrath

Symposium of Student Scholars

With the advent of next-generation sequencing (NGS) on metagenomes, the elucidation of all genetic material from microbiomes has prompted a renewed interest towards uncultivated members of the virosphere. We describe the discovery of a novel phage from a metagenomic dataset on the West African fermented dairy product, nunu, with a custom bioinformatics workflow to potentially serve as a biocontrol agent against pathogenic E. coli. Initial dataset of ERR2014814 from NCBI was first subjected to Kraken2 to extract novel sequencing reads for further de novo assembly into contigs by MetaVelvet. Resultant contigs served as potential partial phage genomes, then searched …

Effectiveness Of Bacteriophages Against Bloater-Causing Bacteria Enterobacter Cloacae In A Model Food System, Ashley Reed, Dzhuliya Ignatova, Sandra Kopic, Unique Sardeneta

Symposium of Student Scholars

Effectiveness of bacteriophages against bloater-causing bacteria Enterobacter cloacae in a model food system

Department of Molecular and Cellular Biology

Ashley Reed, Dzhuliya Ignatova, Sandra Kopic, Unique Sardeneta, and Jean Lu

Abstract

Cucumber fermentation is one of the most important vegetable fermentations in the United States and Europe. Enterobacter cloacae and other gas-producing bacteria can cause bloater defect (the gas pockets or hollow cavities formed in fermented cucumbers) which lowers the quality and the yield of fermented cucumbers, thereby resulting in significant economic losses to the pickling industry. Cost-effective strategies to control E. cloacae and other microbiota need to be …

Effect Of Ph And Heat Treatment On Physicochemical And Functional Properties Of Spray-Dried Whey Protein Concentrate Powder, Thao M. Ho, Jinlei Zhu, Nidhi Bansal, Mary C. Boyce, Thao T. Le

Research outputs 2014 to 2021

Effects of pH and heating on deamidation of whey protein concentrate (WPC) solution and functional properties of resultant spray-dried WPC powder were investigated. Temperature and heating time affected deamidation rates with the highest reactivities for WPC solutions heated at 120 °C for 15 min and 145 °C for 120 s. Deamidation sites were pH dependent: pH 3 induced more glutamine deamidation; pH 10 induced more asparagine deamidation. The functional properties of spray-dried WPC powders were also pH dependent. WPC solution adjusted to pH 3 and heated at 145 °C for 120 s (prior to spray drying) exhibited a reduction in …

Parameterization Of Food Wastes To Develop An Automatic Recycling System For Livestock And Poultry Feed, Ali Roshanianfard, Samira Nematzadeh, Tarahom Mesri-Gundoshmian

Emirates Journal for Engineering Research

Food wastes are known as one of the big concerns in urban management because of grain consumption gain, environmental pollution, and traditional waste management methods. The reuse of restaurant waste can reduce the cost of producing animal food production. This study attempts to find related parameters to use in the development of an automatic recycling machine and also a suitable method for food waste management (wastes of restaurants in universities and other academic environments) to use in various animal diets. Determination of various parameters including the percentage of dry matter (using a dryer), protein (using Kjeldahl test), fat (using Soxhlet …

Clean Label In Bread, Maite Cristina Alava Vargas, Senay Simsek

Department of Food Science Faculty Publications

Bread is considered a staple food worldwide, and therefore there is much interest in research around the topic. The bread industry is usually looking for ways to improve its formulations. Therefore, other ingredients such as dough conditioners, crumb softeners, emulsifiers, and surfactants can be added to enhance bread quality. These ingredients perform functions such as helping standardize processes in the industry, reducing dough-mixing time, increasing water absorption, improving bread quality, and extending its shelf life. Consumers are concerned about the effect of these ingredients on their health, and this has increased the popularity of clean-label bread formulations. A clean label …

Analysis And Performance Evaluation Of Entropic Thresholding Image Processing Techniques For Electrical Capacitance Tomography Measurement System, Josiah Nombo, Alfred Mwambela, Michael Kisangiri

Tanzania Journal of Science

To improve image quality generated from the electrical capacitance tomography measurement system, the use of entropic thresholding techniques is investigated in this article. Based on the analysis of the principle of Electrical Capacitance Tomography (ECT) image reconstruction and entropic thresholding, various algorithms have been proposed for easy extraction of quantitative information from tomograms generated from the ECT system. Experiments indicate that proposed algorithms can provide high-quality images at no or minimum computational cost. It is easier to implement and integrate with classical algorithms such as Linear Back Projection, Singular value decomposition, Tikhonov regularization, and Landweber. Entropic thresholding techniques present a …

Designing A Computer-Vision Application: A Case Study For Hand-Hygiene Assessment In An Open-Room Environment, Chengzang Zhong, Amy R. Reibman, Hansel A. Mina, Amanda J. Deering

Department of Food Science Faculty Publications

Hand-hygiene is a critical component for safe food handling. In this paper, we apply an iterative engineering process to design a hand-hygiene action detection system to improve food- handling safety. We demonstrate the feasibility of a baseline RGB-only convolutional neural network (CNN) in the restricted case of a single scenario; however, since this baseline system performs poorly across scenarios, we also demonstrate the application of two methods to explore potential reasons for its poor performance. This leads to the development of our hierarchical system that incorporates a variety of modalities (RGB, optical flow, hand masks, and human skeleton joints) for …

Analysis Of The Main Chemical And Volatile Components Of Xianggan Tea, Xiang-Style Ganpu Tea, Li Xiang, Fu Fu-Hua, Pan Zhao-Ping, Wang Chen, He Shuang

Food and Machinery

Objective: Xianggan tea, was made from Hunan Anhua dark tea, i. e. Tianjian tea and the mandarin peel of Yueyang Jinpen pomelo, based on the preparation method of Ganpu tea, and this contributed to ascertaining the quality of Xianggan tea. Methods: The main chemical components, mineral element content and volatile components were analyzed in the Tianjian tea, mandarin peel and Xianggan tea. Results: The moisture content of Tianjian tea, mandarin peel, and Xianggan tea were all less than 20%, and made them easy for storage. The content of crude polysaccharides, total flavonoids, and total phenols was in the order of …

Active Components, Antioxidant Capacity And Quality Evaluation Of Sichuan Dark Tea, Zhu Bo-Yu, Xia Chen, Luo Ke-Bin, Zhu Yong-Qing, Tang Xiao-Bo, Chen Jian

Food and Machinery

Objective: The contents of tea polyphenol monomer, caffeine (CAF), total polyphenols acid (TPC) and total flavonoids (TFC) in 26 kinds of commercial Sichuan dark tea (S1～S26) were analyzed, and the in vitro antioxidant activities were investigated, to provide a theoretical basis for the quality evaluation of Sichuan dark tea. Methods: Gradient elution was used to analyze the content of tea polyphenol monomer and caffeine. The contents of TPC and TFC were determined by the folin phenol method and chemical analysis method, respectively. The antioxidant activity was evaluated by DPPH and ABTS radical scavenging capacity. Results: The content of gallic acid …

Effect Of Jet Milling On The Physical And Chemical Properties Of Tartary Buckwheat Powder, Zhang Xue, Zhang Dong-Jie, Zhang Ai-Wu

Food and Machinery

Objective: The effect of superfine grinding on the physicochemical properties of tartary buckwheat powder in different parts was studied, aiming to provide a certain theoretical reference for the food industry to use superfine grinding to prepare tartary buckwheat food. Methods: Tartary buckwheat skin powder, core powder and whole powder were used as materials to prepare tartary buckwheat ultrafine powder by superfine grinding combined with a fluidized bed jet mill. The superfine powder was studied by measuring the particle size, specific surface area, comprehensive powder characteristics, swelling property, water solubility and other indicators. Results: The particle size of the three kinds …